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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Microbiology Indonesia Agrovigor: Jurnal Agroekoteknologi Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Buletin Palawija AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Entomologi Indonesia Jurnal Penelitian Tanaman Industri (Littri) Buletin Palawija

TRI ASMIRA DAMAYANTI

Departemen Proteksi Tanaman, Fakultas Pertanian IPB, Jln. Kamper, Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia, 16680

Author-ID : 249190

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

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Preparasi RNA Virus Mosaik Bergaris dari Tanaman Tebu Menggunakan Metode Tabung PCR Tri Asmira Damayanti; Lilik Koesmihartono Putra
Jurnal Fitopatologi Indonesia Vol. 8 No. 1 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Sugarcane streak mosaic virus (SCSMV) is a new emerging virus infecting sugarcane in Indonesia. The virus can be found almost in every sugarcane plantations in Java and it was known as sett-borne virus. Attempt to get virus-free seedling meet difficulties due to lacking an easy, and accurate detection method. SCSMV antiserum is not available yet commercially. Nucleic acid detection by RT-PCR also hampered difficulties in releasing RNA virus from sugarcane tissues. Here we reported the simple direct tube method with minor modification as the convenient way to provide total RNA template from infected sugarcane leaf, stalk and sheath for RT-PCR detection of SCSMV.Key words: RNA extraction, RT-PCR, SCSMV, tube PCR

Evaluasi Tiga Metode Preparasi RNA Total untuk Deteksi Turnip mosaic potyvirus dari Benih Brassica rappa dengan Reverse Transcription-Polymerase Chain Reaction Jati Adiputra; Sri Hendrastuti Hidayat; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Turnip mosaic virus (TuMV) is an important seedborne virus that infects vegetable crops, especially Brassicae group, in Indonesia. Reliable detection method having high accuracy is required to detect the virus from seeds in order to avoid disease incidence as early as possible. A study was conducted to evaluate three total RNA extraction methods from Brassica seeds for TuMV detection using reverse transcription- polymerase chain reaction. The samples used are Brassica rappa seeds and seedling germinated for 3, 5, and 7 days. Total RNA extraction methods evaluated consisted of Wylie method, Randles method, and commercial kit as comparation. Total RNA extracted using Wylie and Randles methods has high concentration but low level of purity, on the other hand total RNA obtained using commercial kit has low concentration but high level of purity. Detection of TuMV from seed was successfully carried out using Wylie and Randles methods, TuMV from 3 - day old seedlings was detected using all three methodes, TuMV from 5-day old seedlings was only detected using Randles method, whereas TuMV from 7-day old seedlings was not detected using all three methodes. Total RNA extraction method using Wylie and Randles methodes is recommended for detection of TuMV from caisin seed using RT-PCR technique. Key words: Brassica rappa seed, reverse transcription- polymerase chain reaction, Total RNA, Turnip mosaic potyvirus

Lima Ekstrak Tumbuhan untuk Menekan Infeksi Bean common mosaic virus pada Tanaman Kacang Panjang Lulu Kurnianingsih; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 8 No 6 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bean common mosaic virus (BCMV) is one of major virus infecting legumes and is difficult to manage. Utilization of plant extracts as systemic resistance inducer against virus is needed to study. The aim of the research is to evaluate the potency of five leaf extracts, i.e. from pagoda flower, spiny amaranth, four o’clock flower, Chenopodium amaranticolor, and herba andrographitis against BCMV. The effectiveness of leaf extracts were tested by spraying yard long bean leaves. Plants treated by spine spinach shown varied symptoms, while other treatments showed mild mosaic up to symptomless. The highest to lowest of disease incidence was showed by crude leaf extract of spine spinach (70%), four o’clock (10%), herba andrographitis (10%), while C. amaranticolor and pagoda are still uninfected. These results had positive correlation to disease severity and virus inhibition. Four of five tested leaf extracts, except spine spinach, showed their potency as systemic resistance inducer against BCMV. Key words: BCMV, plant extract, yard long bean

Deteksi dan Identifikasi Potyvirus pada Ubi Jalar di Tana Toraja, Sulawesi Selatan Laras Anjarsari; Gede Suastika; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Typical viral symptoms including chlorotic and uneven interveinal yellowing on leaves without leaf malformation was observed on sweet potato field in Tana Toraja, South Sulawesi. To identify the causal of the disease, reverse transcription-polymerase chain reaction (RT-PCR) and DNA sequencing were carried out to detect the virus from infected plants. RT-PCR using universal primer for C1 gene of Potyvirus was successfully amplified approximately 683bp DNA fragment. The nucleotide sequences of this C1 gene fragment showed 98% homology to Sweet potato virus G (SPVG). Amplification using specific primer for coat protein (CP) gene of SPVG followed by DNA sequencing confirmed the association of SPVG from infected plants. Further nucleotide analysis shwowed that SPVG isolate from Tana Toraja had 99.2% homology to isolate from Japan. This is the firstt report of SPVG infection on sweet potato in South Sulawesi.

Infeksi Bean common mosaic virus pada Umur Tanaman Kacang Panjang yang Berbeda Hamdayanty Hamdayanty; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 10 No 6 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bean common mosaic virus (BCMV) is one of the most important virus infecting yard long bean because it can decrease yield and seed transmitted. The aims of research were to determine the effect of plant age infected by BCMV on seed transmission efficiency of the virus, as well as its effect on plant growth. BCMV was mechanically inoculated on yard long bean at 1, 2, 3, and 4 weeks after planting (WAP). Observation was conducted for incubation period, disease incidence and severity, seed transmission efficiency of the virus, plant height, and productivity of the plants. Virus infection was detected using indirect ELISA method. The results showed that the earlier infection of BCMV occurred the shortest incubation period, the most severe symptoms, the highest inhibition of plant growth, and productivity. Disease severity was 94.6, 83.8, 81.1, and 69.6% on plants inoculated at 1, 2, 3, and 4 WAP, respectively. Disease incidence and virus titer was not affected by infection on different plant age. Seed transmission of BCMV was 7, 66, 39, and 24% on plants inoculated at 1, 2, 3, and 4 WAP, respectively. Infection on 2 WAP was considered the critical times for BCMV to be seed-borne on yard long bean.

Penyakit Mosaik pada Koro Pedang Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 11 No 1 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Recently, a mosaic disease was observed on jack bean cultivation in Dramaga, Bogor,West Java. The symptom on infected plants, i.e. combination of pale and dark green area was more obvious on young leaves. Vein banding, leaf malformation, and growth inhibition was also observed in infected plants. The symptom was not very obvious in older leaves. Serological detection gave positive reaction to Bean common mosaic virus (BCMV) antiserum, but reaction was negative when detected using specific primer to coat protein (CP) of BCMV strain black eye by reverse transcription polymerase chain reaction. DNA fragments were successfully amplified by RT-PCR using universal primer of CP gene and degenerated primer for cylindrical inclusion gen (CI) of Potyvirus. DNA analysis showed the highest homology (86.4–91.5%) to several isolates of BCMV-infecting different crops from several countries based on partial sequence of CP, whereas based on partial sequence of CI the highest homology (87.3%) was to BCMV-RU1-P. This is the first report of BCMV infection on jack bean in Bogor, West Java, Indonesia.

Deteksi Virus yang Menginfeksi Kedelai di Jawa Yunita Fauziah Rahim; Tri Asmira Damayanti; Munif Ghulamahdi
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Virus infection is an important production constraint for soybean. Research was conducted to detect virus infection from soybean samples collected from several locations in Java. Leave samples from 50 plants was taken randomly from each location in Bogor, Cirebon, Bantul, and Ponorogo. Field symptoms was observed and disease incidence was determined based on serological assay using specific antibodies to Cucumber mosaic virus (CMV), Soybean mosaic virus (SMV), Cowpea mild mottle virus (CPMMV), and Bean pod mottle virus (BPMV). Incidence of CMV, SMV, and CPMMV was 72–84%, 14–24%, and 6–8%, respectively; whereas infection of BPMV was not found. Specific viral DNA of CMV, Potyvirus, and Geminivirus was successfully amplified using specific primer for CMV coat protein, universal primer for Potyvirus and Geminivirus, respectively. Nucleotide sequence analysis showed that isolate CMV from soybean has the highest homology (99%) to CMV strain S, Potyvirus isolates has the highest homology (90%) to BCMV isolate Mungbean from China and BCMV strain Blackeye from Vietnam, and Geminivirus isolates has the highest homology (96%) to Pepper yellow leaf curl virus (PYLCV) from Bogor and Java. Phyllogenetic analysis showed that CMV strain S formed

Deteksi dan Identifikasi Dickeya sp. sebagai Organisme Pengganggu Tumbuhan Karantina A2 pada Tanaman Kentang di Jawa Haerani Haerani; Abdjad Asih Nawangsih; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Erwinia chrysanthemi (currently Dickeya sp.) is one of the A2 quarantine pest that must be concerned of its distribution on potato in Indonesia. The aim of this study is to detect and identify E. chrysanthemi from potato in Java. A total of 400 samples of potato plants showing symptoms of soft rot were obtained from several potato areas in Pangalengan and Garut (West Java), Dieng (Central Java), and Batu-Malang (East Java). Disease incidence was determined by indirect enzyme-linked immunosorbent assay (I-ELISA) using polyclonal antiserum. E.chrysanthemi was isolated from plant samples with positive ELISA results. Furthermore, bacterial isolates were characterized by GEN III OmniLog ID System and PCR using specific primers Ec3F/Ec4R, as well as the universal 16S rRNA primer pair of 27F/1429R. The incidence of E. chrysanthemi based on ELISA was obtained. Based on physiological characters; Gram, catalase, oxidase, and oxidation-fermentation, there were 4 isolates similar to the genus of Erwinia. However, the results of Gen III OmniLog System, PCR, and nucleotide sequences analysis of 16S rRNA confirmed that none of the isolates were identified as E.chrysanthemi. Otherwise, those 4 isolates were identified as Pseudomonas oryzihabitans, Pantoea agglomerans, and Pseudomonas viridiflava. The result of this study indicated that the existence of E. chrysanthemi as an A2 quarantine pest on potato in Java can not be confirmed and remains as an A1 quarantine pest.

Komparasi Metode Isolasi DNA Patogen Antraknosa dan Bulai untuk Deteksi PCR Ade Syahputra; Kikin Hamzah Mutaqin; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Polymerase chain reaction (PCR) is an important tool for detection, identification and monitoring of quarantine pests in Indonesia. DNA isolation method from target organism is an important step to provide adequate DNA template for performing PCR. Objective of the research was to compare conventional, commercial kit, FTA-card and its modification methods of DNA isolation to be used in PCR detection for Colletotrichum acutatum and Peronosclerospora sorghi from chili and maize, respectively. DNA obtained from various isolation methods were measured using UV-vis nanodrop-spectrophotometry. DNA amplification was performed using DNA concentration of 15 ng µL-1 from each isolation method with gradual primer concentrations of 0.4; 0.6; 0.8; and 1.0 mM. The highest concentration of DNA was achieved with conventional methods for C. acutatum from pure culture and P. sorghi from maize leaf. Best DNA purity was obtained from isolation method using commercial kit for C. acutatum from infected fruit (1.94) and from conventional method for C. acutatum from pure culture (1.91). The highest total yield of isolated DNA was achieved by modified FTA-card for C. acutatum from pure culture. In general DNA amplification using various primer concentration gave positive results although DNA bands intensity was varied from faint to very bright. Furthermore PCR optimization using the best primer concentration from previous reaction showed that all DNA templates resulted in thick and bright DNA bands.

Isolasi, Seleksi, dan Identifikasi Bakteri Endofit sebagai Agens Penginduksi Ketahanan Padi terhadap Hawar Daun Bakteri Ida Parida; Tri Asmira Damayanti; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 12 No 6 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae is one of the major problems in rice production in Indonesia. One of the control measures for the disease is by the utilization of endophytic bacteria. This study was aimed to determine the ability of endophytic bacteria isolated from the roots, stems and leaves of rice in inducing plant resistance to bacterial leaf blight on rice. Screening of endophytic bacteria showed that 370 isolates have good viability and have different colony morphology. Among them, 7 isolates were able to induce resistance and 1 isolate was able to promote the growth of rice in the nursery. However, only 8 isolates did not cause hypersensitive reaction on tobacco plants. The selected isolates of endophytic bacteria were further examined for their ability to induce resistance of rice in greenhouse experiments. Observation involved several variables, i.e. PR1 and PBZ1 gene expression, peroxidase enzyme activity, incubation period, and disease progression. Seven isolates of endophytic bacteria were able to induce PR1 and PBZ1 gene expression, 4 isolates were able to increase peroxide enzyme activity, 4 isolates could prolong the incubation period, and 2 isolates can inhibit the development of disease. However, only EB4 451 isolate was consistently able to induce PR1 and PBZ1 gene expression, increased peroxide enzyme activity, prolonged incubation period, and suppressed the progression of the disease. The EB4 451 isolate was identified based on nucleotide sequence as Bacillus subtilis.

Co-Authors . Giyanto . Haryanto Abdjad Asih Nawangsih Ade Syahputra Akhiruddin Akhiruddin Anastasya Hondo Anastasya Hondo Annisa Nur Imamah Cahyati, Iwe Dewa Gede Wiryangga Selangga DEWI SARTIAMI Dita Megasari Dwi Subekti Efendi, Darda Efi Toding Tondok Endah Muliarti Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Farida, Naimatul Gede Suastika Gede Suastika Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Giyanto Giyanto Giyanto Giyanto Gusti Ngurah Alit Susanta Wirya Haerani Haerani Hagia Sophia Khairani Hamdayanty Hamdayanty HENDRA PARDEDE I Dewa Nyoman Nyana Ida Parida Imamah, Annisa Nur Imaniasita, Vidya Irwan Lakani IRWAN LAKANI Islami, Nisa Fadhila Januarsih, Vera Jati Adiputra Kartika Catur Damaiyanti Ketut Ayu Yuliadhi Kikin H Mutaqin L Pebriyeni Ladja, Fausiah T. Laras Anjarsari Lilik Koesmihartono Putra Listihani, Listihani Lulu Kurnianingsih Martha Theresia Panjaitan Melinda . Mhd Rifqi Abdillah Miftakhurohmah . Miftakhurohmah Miftakhurohmah MIFTAKHUROHMAH MIFTAKHUROHMAH MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH Mimi Sutrawati Muhammad Fikri Hafizh Munif Ghulamahdi N Mattjik Ni Putu Pandawani Niken Nur Kasim, Niken Nur Niky Elfa Amanatillah NISA RACHMANIA MUBARIK Nur Unsyah Laili Nurfadillah NURHAJATI MATTJIK OLUFEMI JOSEPH ALABI Pratiwi, Rizky Nomi Purnamawati, Iis Purwono Purwono Putri, Maharani Mustika R Kartika RAUF, AUNU RAYAPATI ADIKESAVULU NAIDU Rita Noveriza Rita Noveriza Rizki Haerunisa S Susanto Slamet Susanto Sofranita Syifa Fitriyati SRI HENDRASTUTI HIDAYAT Sugeng Santoso Sugeng Santoso Supramana Suryo Wiyono Syaiful Khoiri TRISNA AGUNG PHABIOLA Wahyudin, Denih Wieke Mei Dina Yunita Fauziah Rahim Yunita Fauziah Rahim Yusmani Prayogo Zulfi, Fahma Zakiya

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