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Association of Growth Hormone (GH) Gene Diversity with Quantitative Characteristics in KUB Chicken Using PCR-RFLP Method Sidik, Wahid Hasyim; Depison, Depison; Gushariyanto, Gushariyanto
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 12 No. 3 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i3.p236-257

Abstract

The length of the title is recommended to not exceed fifteen words. This study aimed to obtain GH gene diversity and GH gene association. The materials used were 96 KUB chickens and 96 blood samples of 43 males and 53 females. The research method was experimental. The data collected included body weight, weight gain, and body size of male and female KUB chickens as well as the diversity of GH genes and the association of GH genes: T-test, principal component analysis (MCA), T2-Hotelling test, genotype frequency, alleles, Hardy-Weinberg equilibrium, heterozygosity and polymorphic information content (PIC). The results showed that body weight at 2 and 3 months of age, body weight gain at 2 to 3 months, and body size of male KUB chickens at 3 months of age were significantly different (p<0.05) higher than female KUB chickens. Body weight, body weight gain, and body size of male KUB chickens were higher than female KUB chickens. Body size characteristics of male and female KUB chickens were chest circumference, and male body shape characteristics were breast length and upper body length in females. The GH|MspI gene of KUB chickens is polymorphic and has associations with body weight, weight gain and body size with the best genotype being +/+.
Pelatihan Produksi Nugget Berbasis Daging Ayam Kampung pada Kelompok Peternak Ayam di Desa Sidelego-Merangin Azis, Abdul; Afriani, Afriani; Lukman, Haris; Nelwida, Nelwida; Berliana, Berliana; Nurhayati, Nurhayati; Muthalib, R A; Depison, Depison; Alwi, Yun; Insulistyowati, Anie; Handoko, Heru
E-Dimas: Jurnal Pengabdian kepada Masyarakat Vol 15, No 3 (2024): E-DIMAS
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/e-dimas.v15i3.17088

Abstract

The recognition of processing chicken nuggets by the group of farmers in Sidelego village is an alternative to increase added value from the use of meat from the old chicken. This community service aims to increase the knowledge and skills of the farmers in Sidelego village utilizing chicken meat as a chicken nugget product. The community service activity involved 20 participants from a farmer's group in Sidelego village. The procedure for this activity is designed in 4 stages, including the preparation, training, demonstration, and evaluation of the activity. The farmers' first evaluation showed that most had consumed chicken nuggets; however, they had never tried to make chicken nugget products. The farmers' response to this activity was perfect, and they actively participated in the delivery of training materials and the practice of making chicken nuggets. The evaluation at the end of the activity, most of the participants (80-90%) were able to understand the materials of the training, and they could make chicken nugget products with a high level of acquisition. About 70% of participants with a moderate acquisition level could design modified nugget products with various kinds or variants, such as nuggets with additional ingredients, such as various types of vegetables or tempeh. Based on the preference test, most of the participants gave an assessment of chicken nugget products in the range of preference levels from the moderate to like a category on the color (80.00%), aroma (80%), taste (70.00%) and texture (80%). It was concluded that most participants (80-90%) understood the process of producing chicken nuggets. The chicken nuggets product can be received very well with the preference level in all categories (color, aroma, taste, and texture).
The Quantitative Characteristics of Cross-Bred Native Chicken (Sentul X Arab Chicken and Merawang X Arab Chicken) Irmaya. H, Dika; Suyadi, Suyadi; Depison, Depison; Ardiantoro, Ari
Buletin Peternakan Vol 48, No 3 (2024): BULETIN PETERNAKAN VOL. 48 (3) AUGUST 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i3.89400

Abstract

The research aims to analyze the production potential and opportunities to increase the productivity of each cross-bred animal based on its quantitative characteristics. The livestock material used in this research was 50 Sera chickens (Sentul chickens x Arabian chickens) and 50 Mera chickens (Merawang chickens x Arabian chickens). The research was carried out by raising chickens from DOC to 3 months of age. The quantitative data involve body weight, weight gain, and body measurements (Head Length, Head Circumference, Head Height, Neck Length, Neck Circumference, Wing Length, Back Length, Back Height, Chest Length, Chest Width, Shank Length, Shank Circumference, Length Third Finger and Pubic Bone Distance). The quantitative data that has been observed is then analyzed through the t-test. Then, PCA is used to identify body shape and size determinants in Sera and Mera chickens. Statistical analysis was carried out using Minitab software version 21. The results obtained showed that the body weights of Sera and Mera chickens were significantly different (p<0.05) from DOC to 3 months of age. A significant increase in productivity in crosses between Sentul chickens and Arabian chickens and Merawang chickens with Arabian chickens occurs starting at 1-2 months. Sera and Mera chickens' body sizes differed significantly (p<0.05). The total diversity of PC1 in Sera chickens was 79.8%, and in Mera chickens, it was 70.6%. Meanwhile, the total PC2 diversity of Sera chickens was 17.7%, and Mera chickens were 7.9%. In conclusion, Sera chickens have the highest quantitative characteristics compared to Mera chickens, and the highest body weight gain occurs at 1-2 months in both Sera and Mera chickens.  Based on principal component analysis. The body size characteristic of Sera chickens is shank length, while in Mera chickens, it is chest length. The distinct shape of Sera chickens is body height, while in Mera chickens, it is shank length.
Association of Growth Hormone Genes with Performance of Crossbreeds of Sentul Chicken and Arab Chicken Using PCR-RFLP Sinaga, Josua; Depison, Depison; Gushairiyanto, Gushairiyanto; Harahap, Ratna Sholatia
Jurnal Agripet Vol 25, No 2 (2025): Volume 25, No. 2 October 2025
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v25i2.42764

Abstract

This study evaluated the body weight, weight gain, body measurements, and genetic diversity of the Growth Hormone (GH) gene in male and female Sentul-Arab (SeA) crossbred chickens. It also explored the association between the GH gene and these traits. The research involved 30 male and 30 female SeA chickens reared from day-old chicks (DOC) to four months of age. The experimental method used direct observation to collect data, including body weight, weight gain, body measurements, and blood samples. Data were analyzed using t-tests, the Hotellings T test, and Principal Component Analysis (PCA). Molecular analyses assessed genotype and allele frequencies, Hardy-Weinberg equilibrium, heterozygosity levels, and Polymorphic Information Content (PIC). The results indicated that male SeA chickens had significantly higher (P0.05) body weight, weight gain, and body measurements compared to females. GH gene analysis revealed polymorphism, with genotype frequencies of +/+ (0.43), +/- (0.35), and -/- (0.22), consistent with Hardy-Weinberg equilibrium. The heterozygosity levels indicated moderate genetic diversity, while the PIC value fell within the low category. In conclusion, male SeA chickens exhibited superior body weight, weight gain, and body measurements compared to females, with chest circumference and shank length being critical indicators of body size. The polymorphic GH|TaqI gene was associated with body weight, weight gain, and body measurements, with the +/+ genotype showing the most favorable effects.
Characteristics of Mudgrovel Crabs Caught Using Trap Fishing Gear in Lambur Luar Village, Muara Sabak Timur District Destiani, Dinda; Depison; Magwa, Rizky Janatul; Lisna; Ramadan, Fauzan; Leni, Yusyam
Journal of Fish Health Vol. 5 No. 4 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i4.8550

Abstract

Mangrove crabs are a type of crustacean that lives in mangrove and estuary ecosystems. one of the freshwater fishery products that have high economic value that are often caught in Lambur Luar Village using trap fishing gear. Purpose of this study was to describe the characteristics of mangrove crabs caught using traps in Lambur Luar Village, Jambi Province. This study was conducted from February 1-16, 2025. The research method used was a census, with data collection techniques using probability sampling. The data observed included the type of mangrove crab, weight, carapace length, carapace width, and sex. Differences in weight, length, and carapace width of mangrove crabs were analyzed using ANOVA followed by Duncan's test at a significance level of 5%. The results showed that there were three species of mangrove crabs caught, namely Scylla serrata, Scylla olivacea, and Scylla tranquebarica. After ANOVA test and Duncan's further test, it was found that the carapace length between Scylla serrata, Scylla olivacea, and Scylla tranquebarica was significantly different (p≤0.05). while the carapace width and weight were not significantly different (p>0.05). Variations sex ratio in the Scylla serrata species were (0.78:0.22) and Scylla tranquebarica (0.67:0.33), indicating that the male population was more dominant in these two species. While the Scylla olivacea species, the sex ratio was almost balanced, namely (0.51:0.49). The conclusion of this study is that the characteristics of mangrove crabs differ between males and females, and there are significant differences in carapace length between mangrove crab species found in Lambur Luar Village.
Co-Authors ., GUSHARIYANTO A. S. Wilastra Abdul Azis Abdul Azis Ade Octavia Adelia, Siska Adriani Adriani ADRIANI ADRIANI Adzitey, Frederick Afriani Afriani Agus Susilo Ahmad Taofik Almakmum, Husni Amalia Fildzah Ghassani Anak Agung Istri Sri Wiadnyani Anie Insulistyowati, Anie Annisa Rianti Ardi Novra Ardiantoro, Ari Arfiana, BS Monica Asa Bela Sri Reformasi Nala Putri Asa Bela Sri Reformasi Nala Putri Asep Gunawan Avrilliani, Puspita B., SUPRIAWAN Bawah , Juliana Bayu Rosadi Bella Pratiwi Putri Berliana Berliana Berliana Berliana Cece Sumantri Dea Tri Ananda Destiani, Dinda Dika Irmaya E. Wiyanto Eko Wiyanto Eko Wiyanto Eko Wiyanto, Eko Endri Musnandar Erina, S. Ester Restiana Endang Gelis Fauzan Ramadan Fetty Febriana Rahayu Fitra Diansah Gushairiyanto Gushairiyanto Gushariyanto Gushariyanto Gushariyanto Gushariyanto Gushariyanto, Gushariyanto H. Ediyanto H. Suryani Haris Lukman Hariski, M Helmi Ediyanto Heru Handoko Heru Handoko Hidayat, Gradiandri Hosen, Tanjir Hossain, Md. Murad Husni Almakmum Irmaya. H, Dika Islam, Nazmul Jalius Jalius L. H. M. Gultom Leni, Yusyam Lisna Lisna Lisna Lisna Lisna, Lisna Lisna, Lisna M., SAMIUN Mithun, Mahmudul Hasan Monten, Stephen K. Kanten Muhammad Abdu Muhammad, Mus'ab Muqsita Rahmat Nabilah Ika Puteri Nasution, Annio Indah Lestari Nelwida Nelwida Nurhayati Nurhayati Nurul Huda Pakpahan, Roberyo Pratama, Bondan Pratama, Shera Aditia Prayoga Alfano Putri, Asa Bela Sri Reformasi Nala R. A. Muthalib Ratna Sholatia Harahap Redo Prawira Rhaphon Seprian. Ht Riris Roiska Rizky Janatul Magwa S. Erina Salsabila Salsabila Septy Heltria Setiyawan, Khoirudin Anton Shalsabila Novia Al-Sobri Sidik, Wahid Hasyim Silvia Erina Silvia Erina Silvia Erina Silvia Erina Silvia Erina Silvia Erina Sinaga, Josua Sofi Crisdayanti Suyadi Suyadi Talukder, Md. Khyrul Alam Triana Mardiah Utama, Millisani W. P. B. Putra Wahidu Zzaman Wahyuni Wahyuni Widya Pintaka Bayu Putra Wiwaha Anas Sumadja Y. Alwi Yun Alwi Zafitra A.