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Fevria, Resti
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Journal : Indonesian Food Science and TechnologyJournal

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Isolation and Identification of Lactic Acid Bacteria Using PCR Gene from Tempe Wrapped with Banana Leaves and Plastic Fevria, Resti; Vauzia, Vauzia; Putri, Dwi Hilda; Achyar, Afifatul; Putri, Santi Diana; Edwin, Edwin
Indonesian Food Science and Technology Journal Vol. 7 No. 2 (2024): Volume 7. Number 2, July 2024 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v7i2.32503

Abstract

Tempe is a typical Indonesian food that comes from fermenting soybeans with the fungus Rhizopus sp. Tempe is known to have good nutritional value because of the content contained in soybeans themselves and other microorganisms that appear as a result of the tempe fermentation process. The fermentation process increases the activity of bacteria in tempe which are beneficial for digestion, one of which is lactic acid bacteria. This research aims to see the differences morphological forms  and genomic of lactic acid bacteria produced by tempe wrapped in banana leaves and tempe wrapped in plastic. The differences in fermentation that occurred in tempe wrapped in banana leaves and those wrapped in plastic resulted in differences in lactic acid bacteria type. Based on the isolation of bacteria on Mann de Rogosa Sharpe Agar medium, 15 isolates of lactic acid bacteria were produced, with general morphological forms of bacilli and coccus. Then, genomic identification was carried out using the PCR. The phylogenetic tree built based on the 16S rRNA gene sequence can show the relationship between LAB diversity at the species level, but cannot differentiate LAB from the strain level.
Co-Authors -, Vauzia - -, Yulkifli - A. Ardi Abdul Razak Abdul Razak Achyar, Afifatul Afiah, Annisa Afrilisia, Lora Afrini Dona Agnes Ramelin Novelina Alda Viona Alqaramah, Rachel Amalia Fildzah Sharfina Andi Alatas, Andi Annisa Annisa Annisa Tulhasanah Annisa, Silvy Ardi Ardi Ardi Ardi Atifah, Yusni Audela Oktavira Aufa Rafiqi cynthia perdana putri Delvia Suarman Des M Des M Des M. M. Dezi Handayani Dhea Sabrina Aprilia Dwi Hilda Putri Edwin Edwin Edwin Edwin Edwin Edwin Edwin Edwin Eliza Octa Khairani Elsa Badriyya elva rahmi Eni Kamal Erwina Simanullang Fadilla - Meidita Fadilla Meidita Fadilla Sonia Putri Fajri Rifyant Fitria Anwar Ghiffari, Muhammad Helendra . Humulusna, Rira Indiastri P Monica Indra Hartanto Indra Hartanto Irdawati Irdawati Irdawati Irdawati Irma Lailani Putri Irma Leilani Eka Putri Isril Berd Kiki Amelia Linda Advinda M Maizirwan Maiyontoni Maiyontoni Marni, Yunis Mitason, Deka Moralita Chatri Muhammad Ghiffari nada wafiq hijriah, Nada wafiq Nia Faradila Niken Maharani Putri Novita Sukawati Nurul Fadilah Oktaviani, Mutia Olan Tri Sinaga Purnamasari, Devi Putri Oktavia Putri, Afriani Amelia Putri, Santi Diana Qurrata A'yuni A'yuni Rahmadhani Fitri Ramadhan Sumarmin Rara Via Jonet REFIKA KOMALA Reki Kardiman Rijal Satria Rika Andima Rini Elisia Ririn Indria Sari Ristiono Ristiono Rizka Meisy Evis Putri Rizki Rahman Putra Roisiah, Qoimatun Roni Jarlis S. Syamsurizal S. Syamsurizal Sania, Hani Selaras, Ganda Hijrah Sintia Delia Syafitri Siska Farma Sri Mutiar, Sri Supriyanti, Juli Susalam, Malikil Kudus Syamsurizal Syamsurizal Syuhada, Fildza Arief Tazri, Mufidah Insani Vauzia Vauzia Vauzia Vauzia, Vauzia Vauzia, Vauzia Violita Violita Violita Violita Violita Violita Viona, Alda Vivi Hendrita Wahyudi Rahmat Wanda Arjulis Wanda Arjulis Wilna Sari Wita Febriani Yovella Trisna Wijaya Yulkifli Yulkifli Yurico Utami Zulfa Febriani