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All Journal Bioscience Pendidikan Biologi Biocelebes Jurnal Penelitian Pendidikan IPA (JPPIPA) Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Biologi Tropis Jurnal Agrotek Tropika JURNAL PENDIDIKAN TAMBUSAI FONDATIA AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Pelita Eksakta Sainstek : Jurnal Sains dan Teknologi Jurnal Abdi Insani Journal On Teacher Education (Jote) International Journal of Education and Literature (IJEL) MASALIQ: Jurnal Pendidikan dan Sains Jurnal Serambi Biologi
Irdawati Irdawati
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
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Journal : Bioscience

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Starter Concentration of SSA4 Thermophilic Bacteria in Producing Xylanase Irdawati Irdawati; Jannah Koftiah; Dwi Hilda Putri; Linda Advinda
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371117423-0-00

Abstract

Advances in the field of biotechnology have led to the use of enzymes in industry increasingly. The high public awareness of environmental pollution makes enzyme technology as an alternative to replace various chemical processes. Enzymes can be used in various industrial sectors such as textiles, food, detergents, paper and cosmetics, as well as biofuels. One of the enzymes that is often used is the enzyme xylanase. Thermophilic microorganisms that can produce thermostable enzymes are thermophilic bacteria. One of the important factors affecting the success of the manufacturing process of the production of the xylanase enzyme is the concentration of the starter. The production of the thermophilic bacterial xylanase enzyme SSA 4 produced the highest specific enzyme activity at a concentration of 4% which was 0.137 U/mg. This shows that low starter concentrations can overwhelm microbial growth well. The increase in the concentration of the starter causes the enzyme activity to decrease, this happens.
The Potential of Hot Water Sapan Sungai Aro Thermophilic Bacteria Consortium in Producing Bioethanol Inayatul Fatia; Irdawati Irdawati; Linda Advinda; Azwir Anhar
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.123265

Abstract

Biofuel is a potentially renewable alternative fuel in Indonesia. Bioethanol is one example of the most commonly used biofuel. Microorganisms of thermophilic bacteria are known to contribute to the production of bioethanol. Thermophilic bacteria are efficient against high temperature conditions so as to minimize contamination. Production of bioethanol can also use joint culture (consortium). Bioethanol production using a microbial biculture consortium is known to significantly increase the level of bioethanol production. The purpose of this study was to determine the compatibility and to determine the optimum potential of the thermophilic bacterial biculture consortium of Sapan Sungai Aro hot water for bioethanol production. This research is a type of descriptive research. To test the cooperation between consortium isolates of thermophilic bacteria producing bioethanol, a compatibility test was carried out using the disk diffusion method. Then the consortium isolates were fermented in liquid TMM (Thermophilic Minimum Media) medium, the bioethanol content was measured after distillation using a pycnometer. The results of the bacterial compatibility test showed that there was one pair of isolates that were not compatible, namely SSA 8 & SSA 14 due to the presence of a clear zone. On research results. The production of bioethanol by a consortium of thermophilic bacteria gives more optimal results compared to a single isolate. The best thermophilic bacterial biculture consortium from the Sapan Sungai Aro hot spring in producing biofuels is SSA 14 & SSA 16 which is 3.009%. 
PRODUCTION OF SPESIFIC XYLANASE ENZYME BY MUDIAK SAPAN HOT WATER THERMOPHILIC BACTERY Irdawati Irdawati; Muhammad Deedat Ayasy; Azwir Anhar; Linda Advinda; Yusrizal Yusrizal
Bioscience Vol 8, No 1 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i1.122728

Abstract

Xylanase is an extracellular enzyme capable of hydrolyzing hemicellulose so that it can convert xylan into xylose. Thermostable xylanase enzymes can be produced by thermophilic bacteria. Thermophilic bacteria are used because they have advantages such as the ability to increase enzyme production in adjustable catalytic specifications. Bacteria are not only in a single form but also exist in a mixed form called a consortium. Compared with a single isolate, the performance of the consortium is better. Consortium is a mixture of microbial populations in the form of communities that have cooperative, commensal, and mutualistic relationships. This study aims to look at the cooperation between the consortium isolates and the consortium's ability to produce xylanase enzymes. This research is a descriptive study. The bacterial consortium is fermented in beechwood xylan medium. The results of this study were that MSS 11, MS 18, MS 16 consortium produced the highest xylanase enzyme activity, namely 12,887.Xilanase merupakan enzim ekstraseuler yang mampu menghidrolisis hemiselulosa sehingga dapat merubah xilan menjadi xilosa. Enzim xilanase termostabil dapat diproduksi oleh bakteri termofilik. Bakteri termofilik digunakan karena dapat memiliki keunggulan seperti aktivitas dan peningkatan spesifikasi katalisis yang dapat diatur. bakteri tidak hanya dalam bentuk tunggal namun juga ada dalam bentuk campuran yang disebut konsorsium. Dibandingkan dengan isolat tunggal, kinerja konsorsium lebih baik. Konsorsium merupakan campuran populasi mikroba dalambentuk komunitas yang mempunyai hubungan kooperatif, komensal, dan mutualitsik. Penelitian ini bertujuan untuk melihat kerjasama antar isolat konsorsium dan kemampuan konsorsium dalam menghasilkan enzim xylanase. Penelitian ini bersifat deskriptif. Pada penelitian ini, konsorsium bakteri difermentasikan dalam medium beechwood xylan. Hasil penelitian ini adalah konsorsium MSS 11, MS 18, MS 16 menghasilkan aktivitas enzim xylanase spesifik tertinggi yaitu 12,887.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abror Abror Agustien Agustien Ahmad, Nurafni Fajarna Do Andrian, Richard Anggraeni, Novicka Putri Anthoni Agustien Apriansa, Apriansa Ardi Ardi Armiliandi, Rada Aryeni Fatwah, Monika Az-Zahra, Fauziah Azwir Anhar Bob Refianto Deni Kurniati Des M Des Desi Nurmayenti Dezi Handayani Dezi Handayani Dwi Hilda Putri Elfah rahma Elsa Sri Handayani Fahra Fahra Fahra Fahra Fahra, Fahra Fajri, Dilham Hidayatul FAJRINA, SUCI Faradilla Rattriana Fatiha, Fathma Dwi Fauzana Ahmad Feby Yeriska Fevria, Resti Fitri Angraini Fitri Arsih Francantika, Manja Ghiffari, Muhammad Gustina Indriati Heffi Alberida Helpida Helpida Ilsa Septia Putri Inayatul Fatia Indrawani Matondang Irma Leilani Jannah Koftiah Khalif Alfajri Kurnia Wulansari Larashinda, Mentari Linda Advinda Lufri Lufri Luthfi, Afdal Mades Fifendy Marissa Angelina Maulina, Adinda Rizky Mei Niarti Melsi Pratiwi Yusni Monhartini, Monhartini Moralita Chatri Muhamad Sholichin Muhamad Sholichin Muhammad Aulia Rahman Muhammad Deedat Ayasy Muhyiatul Fadilah Mulia Mulia Mulia Mulyadi, Rival Muskhir, Mukhlidi Nada Nafion Nia Ramadhanti Nisa Afifah Nissha, Dwi Khayrun Nur Shofiatun Nisa Nur Vaizi Nurfatihah Z, Zahara Putri Andriani Putri Ardila Sari Putri Rachma Auliya Putri Rahma Padilla Putri Sarinami Rahmi, Elva Refni Syahleli Afni Rizka Putri Alti Roza Yolanda Sasmeri Sasmeri Selaras, Ganda Hijrah Sri Wahyuni Suci Fajrina Suherman, Donny Summaiati, Titi Syahrastani, Syahrastani Syamsuardi Syamsuardi Syamsurizal S Tomi Apra Santosa Uthami, Feranis Nadia Vauzia Vauzia Vestimarta, Aldi Wahyuda Violeta Violita Violita Violita Violita Viona, Alda Wahyuda Vestimarta, Aldi Wardah Hayati, Wardah Wella Antika Weni Yulastri Windi Nopitasari Wirda Taufik Witri Nofita Safitri Wulansari, Kurnia Y Rilda Y Yusrizal Yetria Rilda Yuandry, Syafrina Yusrizal Y Yusrizal Y Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zulyusri, Zulyusri