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Qurnianingsih, Ema
Department Of Biochemistry, Faculty Of Medicine, Airlangga University, Surabaya, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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PRELIMINARY LABORATORY EVALUATION OF RAPID DIAGNOSTIC TEST PROTOTYPE TO DETECT HBsAg IN WHOLE BLOOD AND SERUM Qurnianingsih, Ema; Wungu, Citrawati Dyah Kencono; Amin, Mochammad; Setyawati, Dewi; Yasan, Yanna Debby Restifanny; Lukitasari, Lina; Lusida, Maria Inge; Soetjipto, Soetjipto
Folia Medica Indonesiana Vol. 61, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Chronic hepatitis B virus (HBV) remains a major global health issue,[-10pc]AU: Please provide ORCID ID for all authors.[-7pc]AU: Please provide affiliation’s for all authors. with over 250 million people affected worldwide. In Indonesia, HBV prevalence has declined from high to moderate endemicity, but challenges persist due to regional disparities, risk of mother-to-child transmission, and the predominance of B3 and C1 sub-genotype. Despite available preventive measures and diagnostic tools, approximately 90% of cases remain undiagnosed, particularly in low-resource settings, indicating the need for improved screening and control strategies. Therefore, this study aims to conduct preliminary performance tests of KODC hepatitis B surface antigen (HBsAg) (rapid diagnostic test (RDT) prototype) produced by Konimex on serum and whole blood samples. Performance was assessed through sensitivity, precision, specificity, and comparative testing against commercial RDT. The results obtained were visually interpreted by 2 independent readers. KODC HBsAg RDT demonstrated a detection limit of 50 IU/ml in serum and 400 IU/ml in whole blood. Precision testing showed high reproducibility (RSD <3.02%), with no cross-reactivity against other pathogens. Compared with a commercial RDT, it showed equivalent performance in serum and superior specificity in whole blood. In addition, predictive value analysis confirmed excellent accuracy, with Positive predictive value (PPV) and negative predictive value (NPV) >99% in both sample types. Therefore, KODC HBsAg RDT prototype showed comparable performance to commercial tests with good sensitivity, precision, and specificity, indicating its potential as a practical tool for HBV screening in resource-limited settings.
Effects of Ethanol Extract of Rosa damascene on HbA1c Level and NF-κB Expression in Diabetic Rats Choirotussanijjah, Choirotussanijjah; Notopuro, Harianto; Qurnianingsih, Ema
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.2151

Abstract

Diabetes mellitus (DM) is a chronic hyperglycemic that can cause complications in several organs. It could lead glycosylated hemoglobin or HbA1c formation which has ability undergo further changes into Advanced Glycation End Products (AGEs) and stimulate activation of transcription factors such as nuclear factor kappa B (NF-κB). Some studies showed that anthocyanin has antioxidant and anti-inflammatory activity and it has been found Rosa damascene contain high level of anthocyanin. Total anthocyanin content was 0.459 ± 0.003 mg/L. The aim of this study was to investigate inhibitory effect of R. damascena extract on HbA1c levels and NF-κB activation in diabetic rats. Male wistar rats (n=24) were divided into 6 groups as normal control (KN), streptozotocin-induced diabetic control (KP), diabetic treated with R. damascene (P1, P2, P3; 250, 500 and 1000 mg/kg/d respectively) and diabetic treated with metformin (KM; 500 mg/kg/d). This was carried out for 28 days. The inhibition mode of R. damascene extract was examined by measuring HbA1c levels and expression of NFκB by immunohistochemistry. The results showed p values ​​> 0.05 for HbA1c and p < 0.05 for NFκB. From immunohistochemical staining, it seen the expression of NFκB was low in treated group (P1, P2, P3 and KM) compared with KP. Thus, oral administration of R. damascene extract for 28 days could not reduce HbA1c levels, but can supress NFκB expression.
Improving Posyandu Cadres knowledge through stunting education and training on sea fish–based MPASI and PMT preparation in Darungan Village Ira Humairah; Patmawati Patmawati; Ema Qurnianingsih; Lina Lukitasari; St. Khaerunnisa; Fithriyah Cholifatul Ummah; Langgeng Agung Waskito; Citrawati Dyah Kencono Wungu; Imanta Alifia Oktavira; Novita Nur Ramadhani; Dede Sayyidatunnisa; Muhammad Hidayat; Gwenny Ichsan Prabowo
Buletin Pengabdian Bulletin of Community Services Vol. 6 No. 1 (2026): Bull. Community. Serv.
Publisher : LPPM Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v6i1.2

Abstract

Background and Aim: Stunting remains a major public health problem that affects child growth and development. Community-based nutritional interventions utilizing local food resources are important strategies to support stunting prevention. This community service activity aimed to improve the knowledge and skills of Posyandu cadres regarding stunting prevention through education and training on the preparation of sea fish–based MPASI and PMT in Darungan Village. Methods: The program was conducted using educational counseling, practical training, and mentoring activities for Posyandu cadres. Participants received materials on stunting prevention, balanced nutrition, and practical demonstrations of preparing sea fish–based MPASI and PMT. Knowledge improvement was evaluated using pre-test and post-test assessments. Results: The results showed a significant increase in participants’ knowledge regarding stunting prevention and balanced nutrition, with a p-value of 0.025 (p < 0.05). In addition, participants demonstrated improved practical skills in preparing nutritious sea fish–based complementary and supplementary foods for children. Conclusion: Education and training using locally available sea fish as nutritional resources effectively improved Posyandu cadres’ knowledge and practical skills in stunting prevention. This program has the potential to support sustainable community-based nutrition interventions and contribute to achieving Sustainable Development Goal (SDG) 3, namely Good Health and Well-being.
Co-Authors . Kholik Adi, Ginanjar Sasmito Alda Dini Wijayanti Amin, Indah Binti Mohd Amin, Mochammad Anggraeni, Zuhrotul Eka Yulis Arifa Mustika Audi Salman Faza Ayesie Natasha Zulka Bakhtiar, Arief Bayu Satria Wiratama Brahmana, Alisha Princessa Choirotussanijjah, Choirotussanijjah Damayanti Damayanti Damayanti Dede Sayyidatunnisa Eka Arum Cahyaning Putri Elvaretta, Clarissa Ely Rahmatika Nugrahani Ely Rahmatika Nugrahani, Ely Rahmatika Fajar Shamsudin Fajar Syamsudin Fithriyah Cholifatul Ummah Ginanjar Sasmito Adi Gosy Endra Vigriawan Hamzah, Arshq Mirza Harianto Notopuro Hendri Susilo Hera, Renata Evania HERAWATI, LILIK Hermina Novida, Hermina Humairah, Ira I'tishom, Reny Imanta Alifia Oktavira Indri Safitri Indri Safitri, Indri Jefferson Caesario Jemima Lewi Santoso Kadek Rachmawati Khaerunnisa, Siti Lilia Putri Pratama Lilik Djuari Linda Dewanti Lukitasari, Lina Mangestuti Agil Maria Inge Lusida Maridsky, Aldilla Afifie Putri Miranda Jemyma Mas&#039;ulun Muhammad Hidayat Muhammad Hidayat Novita Nur Ramadhani Palupi, Dian Eka Roro Patmawati Patmawati Prabowo, Gwenny Ichsan Pradana, Munawer Purwanta, Marijam Purwitasari, Neny Putri Brillian Betrista Viorizka Ramadhani, Rizqi Nabila Rejeki, Purwo Sri Retno Handajani Rias Gesang Kinanti Riwu, Katty Hendriana Priscilia Rusyda, Aqila sabila Setyawati, Dewi Siti Khaerunnisa Siti Khaerunnisa Soebagijo Adi SOETJIPTO . St. Khaerunnisa Sudibyo, Devi Ariani Suhartati Sulistiawati Sulistiawati Susi Wahyuning Asih Susi Wahyuning Asih, Susi Wahyuning Suwandito Suwandito Suwandito, Suwandito Syafa'ah, Irmi Syafa’ah, Irmi Trisniartami Setyaningrum Ummah, Fithriyah Cholifatul Ummah, Fithriyah Cholifatul Ummah Vania Islamey Kusuma Waskito, Langgeng Agung Wungu, Citrawati Dyah Kencono Yasan, Yanna Debby Restifanny Yuri Widia, Yuri Zachawerus, Anjeli Cristina Zuhrotul Eka Yulis Anggraeni Zulka, Ayesie Natasha