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Penggunaan Biochar dan Dekomposer dalam Proses Pengomposan Limbah Kulit Buah Kakao serta Pengkayaan Mikrob Pelarut Fosfat (MPF) untuk Meningkatkan Kualitas Pupuk Organik: Application of Biochar and Decomposers to the Composting Process of Cocoa Husk and Enrichment Using Phosphate Solubilizing Microbe to Improve the Quality Organic Fertilizer Haryanti Haryanti; Iswandi Anas; Dwi Andreas Santosa; Kurnia Dewi Sasmita
Jurnal Ilmu Tanah dan Lingkungan Vol 20 No 1 (2018): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.487 KB) | DOI: 10.29244/jitl.20.1.25-32

Abstract

The use of cocoa husk waste by farmers is not optimal. In general, farmers only dispose of or immerse cocoa husk waste without processing it into quality organic fertilizer. This study aims to determine the influence of biochar and decomposers in accelerating the process of composting cocoa husk waste and improving the quality of organic fertilizers, and the effect of adding Phosphate Solubilizing Bacteria (BPF) to organic fertilizer derived from cocoa husk waste on the growth of cocoa seedlings (Theobroma cacao L.). The first phase of the research was carried out a factorial 2 factor design in the Completely Randomized Design i.e. Biochar (without biochar and 10% biochar from the dry weight of organic fertilizer materials), and Decomposers (without decomposers and decomposers of 100 g kg-1 of organic fertilizer). The second phase of the study used a 3-factor factorial design in a Completely Randomized Design i.e. type of organic fertilizer (without organic fertilizer, organic fertilizer, organic fertilizer and biochar), inoculant Phosphate Solubilizing Bacteria (BPF), (without BPF inoculants, BPF A inoculants and BPF B inoculants), and dose of P fertilizer (without P fertilizer, SP-36 fertilizer 1 g P2O5, SP-36 fertilizer 2 g P2O5, and natural phosphate fertilizer 2 g P2O5). The results showed that the administration of biochar and decomposers did not accelerate the composting process. Provision of biochar and decomposers increases levels of humic acid. Giving natural phosphate 2 g P2O5 / 2.2 kg BKM increases the height growth of cocoa seedlings. Application of organic fertilizer with biochar or without biochar on the inoculant treatment factors of BPF A and BPF B significantly increased the diameter of the cocoa seedling stem. Application of organic fertilizer increases the stem diameter, plant height, number of leaves of cocoa seedlings, root dry weight and dry weight of stem of cocoa seedlings.
Evaluasi Penggunaan Pupuk Hayati pada Pertumbuhan Tanaman Alpukat (Persea americana Mill.) di Kebun Superavo, Subang: Evaluation of the Use of Biofertilizer on the Growth of Avocado Plants (Persea americana Mill.) at Superavo Orchard, Subang Fahrizal Hazra; Dwi Andreas Santosa
Jurnal Ilmu Tanah dan Lingkungan Vol 24 No 1 (2022): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.24.1.14-19

Abstract

Increasing the amount of avocado production is influenced by the effectiveness of fertilization. Fertilization is the most important thing in agriculture to improve plant nutrition, achieve high yields, and change the soil environment. The use of biofertilizer is needed to improve the quality and quantity of plants and reduce the chance of land degradation. The experimental design used was a randomized block design (RBD) with a single factor in fertilization. This RAK has nine treatment levels, namely: 1) control (without NPK and biofertilizer), 2) 100% NPK fertilizer, 3) 50% NPK fertilizer, 4) 100% NPK fertilizer + 100% biofertilizer, 5) NPK fertilizer 100% + 50% biofertilizer, 6) 50% NPK fertilizer + 100% biofertilizer, 7) 50% NPK fertilizer + 50% biofertilizer, 8) 100% biofertilizer, 9) 50% biofertilizer. This treatment was repeated five times. The use of NPK as a combination and substitution of biofertilizer. The basic fertilizer used, namely manure as much as 1.25 kg/tree. Overall, the best treatment was a combination of NPK fertilizer and biological fertilizer, especially in the P3 treatment, namely a combination of 100% NPK and 100% biological fertilizer. This can be seen in the vegetative growth of avocado plants and the soil's chemical and biological analysis, which have increased. Significant results occurred in plant height, the number of tertiary branches, total N, and soil invertase activity.
Application of Biological Nitrogen Fixation Cyanobacteria To Paddy Plant Cultivated Under Deep-Water Culture System Dian Hendrayanti; Iman Rusmana; Dwi Andreas Santosa; Hamim Hamim
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.8510

Abstract

 The Biological Nitrogen Fixing (BNF) cyanobacteria can reduce atmospheric nitrogen into ammonium. This ability makes BNF cyanobacteria a potential eco-friendly N-source for soil-planted pad-dy. Apart from a few success stories of BNF cyanobacteria applica-tion in the rice field, its role as an ammonium producer is still an open question. There is also a possibility that indeed cyanobacteria biomass which provides nitrogen through the biological decomposing process. This study aimed to analyze the influence of three strains BNF cyanobacteria on paddy grown in the Deep-Water Culture (DWC) hydroponic system. Yoshida Nutrient Solution was used as a growth medium with the application of 0, 45.7, and 91.4 gL-1 of ammonium nitrate (NH4NO3) with five replications. The result showed that inter-action between cyanobacteria and nitrogen significantly influenced the total nitrogen of paddy plant, but not the paddy plant height, the num-ber of tillers, and biomass. Cyanobacterial filaments were found most-ly attached to the paddy root rather than floated in the DWC system. Paddy treated with cyanobacteria had more lateral root than control. The result suggested that cyanobacteria support paddy growth indi-rectly through nitrogen deposition in plant tissue and root development. 
STUDI MIKROBIOLOGI DAN SIFAT KIMIA MIKROORGANISME LOKAL (MOL) YANG DIGUNAKAN PADA BUDIDAYA PADI METODE SRI (System of Rice Intensification) Arum Asriyanti Suhastyo; Iswandi Anas; Dwi Andreas Santosa; Yulin Lestari
Sainteks Vol 10, No 2 (2013): SAINTEKS
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v10i2.148

Abstract

Penggunaan larutan mikroorganisme lokal (MOL) dalam metode SRI budidaya padi yang dikembangkan di Indonesia dimulai awal sejak persiapan bibit fase vegetatif, pembentukan malai dan pengisian bulir padi. MOL adalah cairan yang dapat dibuat dari bahan yang tersedia di sekitar kita seperti sisa sayuran, rebung, keong mas, buah maja, daun gamal, bonggol pisang, nasi, urine kelinci, dan lain-lain. Cairan umumnya diberikan 10, 20, 30, 40 dan 60 hari setelah tanam (HST ) atau sesuai kebutuhan. Penelitian ini menggunakan larutan MOL yang terbuat dari bonggol pisang, keong mas dan urin kelinci. Tujuan penelitian adalah untuk menentukan mikrobaa, identifikasi mikrobaa dan sifat kimia dalam MOL bonggol pisang, keong mas dan urin kelinci. Penelitian ini dilakukan di Laboratorium Bioteknologi Tanah IPB. Penelitian ini menggunakan rancangan acak lengkap dengan satu faktor (waktu) dan tiga kali pengulangan. Hasil penelitian menunjukkan MOL bonggol pisang memiliki rata-rata nilai pH terendah (4,2-4,5) dan nilai EC tertinggi (10,44-12,82 mikrodetik/cm) selama proses fermentasi. MOL keong emas memiliki rata-rata pH tertinggi (4,5-6,55) dan dan yang paling oksidatif - reduktif nilai Eh [ 269-(-381) mV ] selama proses fermentasi. MOL urin kelinci memiliki nilai rata-rata terendah dari EC (2,18-2,23 mikrodetik/cm) dan mengandung lebih banyak unsur K, Ca, Mg, Cu, Zn, Fe dan Mg dari kedua jenis MOL lainnya. Selanjutnya, Bacillus sp., Aeromonas sp. dan Aspergillus niger diidentifikasi dalam MOL dari bonggol pisang. MOL dari keong mas mengandung Staphylococcus sp. dan Aspergillus niger, sedangkan MOL urin kelinci memiliki Bacillus sp. , Rhizobium sp. , Pseudomonas sp. , Aspergillus niger dan Verticillium sp. Kata kunci : SRI, MOL, sifat kimia MOL, identifikasi mikrobaa
ISOLASI DAN IDENTIFIKASI BAKTERI PEREDUKSI SULFAT PADA AREA PERTAMBANGAN BATU BARA MUARA ENIM, SUMATERA SELATAN Muchamad Yusron; Bibiana W Lay; Anas M Fauzi; Dwi Andreas Santosa
Jurnal Matematika Sains dan Teknologi Vol. 10 No. 1 (2009)
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.733 KB) | DOI: 10.33830/jmst.v10i1.569.2009

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Sulfate reducing bacteria utilize sulfate as their terminal electron acceptor and reduce it to sulphide. Acid mine drainage, by-products of mining activities, is an acidic sulfate-rich wastewater suitable habitat for sulfate reducing bacteria. Isolation and identification of sulfate reducing bacteria collected from Muara Enim coal mining, South Sumatra was carried out at Laboratory of Environmental Biotechnology, Indonesian Center for Biodiversity and Biotechnology (ICBB), Bogor, and Laboratory of Microbiology, Faculty of Veterinary, Bogor Agricultural University. Postgate B liquid media was used for isolation and purification via serial dilution. Physiological and biochemical characterization was done based on Bergeys Manual of Determinative Bacteriology. Fifteen pure isolates have been isolated with diverse characteristics. Eight isolates can sustain at pH 3, while the rest sustain at pH 4 or above. Sulfate reduction efficiency of each isolates were different, but increased as the pH increased. The bacteria are classified as Desulfovibrio sp., which is characterized straight rods, motile, non spore-forming and able to grow in simple organic carbon.
Eksplorasi Bakteri yang Berpotensi sebagai Pengendali Hama Ulat Grayak (Spodoptera litura) (Exploration of Potential Bacteria as Biological Control of Spodoptera litura) Wilhelmus Terang Arga Sanjaya; Desak Ketut Tristiana Sukmadewi; Fahrizal Hazra; Aisamrotul Hasanah; Dwi Andreas Santosa
Jurnal Hortikultura Vol 30, No 2 (2020): Desember 2020
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v30n2.2020.p133-140

Abstract

Usaha pengendalian hama ulat grayak (Spodoptera litura) di tingkat petani masih mengandalkan pestisida sintetik. Tujuan penelitian adalah mengeksplorasi bakteri potensial pengendali hama ulat grayak (S. litura) dan menguji ketahanan bakteri potensial pada bahan pembawa kompos dan zeolit. Isolat tanah diisolasi dari tiga jenis sumber, yaitu sampel tanah daerah rhizosfer (padi, kelapa sawit, terung, jagung), sampel buah busuk (kakao, kelapa sawit, jambu air), dan sampel bangkai serangga (ulat api, belalang, kumbang tahi, kupu-kupu) yang diambil dari kawasan Dramaga dengan metode purposive sampling. Penelitian di laboratorium meliputi isolasi bakteri, uji patogenitas, pewarnaan gram, pengamatan morfologi koloni, uji toksisitas, uji biokimia, dan uji bahan pembawa. Berdasarkan penelitian ini didapatkan dua strain yang berpotensi sebagai agens biokontrol dengan kemampuan membunuh hama yang tinggi pada pengujian toksisitas tahap kedua, yaitu IRJ 10 (tingkat kematian 90%) dan ISU 4 (tingkat kematian 100%). Kedua isolat ini merupakan anggota genus Bacillus. Pada uji bahan pembawa kompos dan zeolit, penurunan jumlah sel bakteri pengendali hama paling tinggi adalah pada bahan pembawa zeolit dibandingkan dengan menggunakan bahan pembawa kompos. Jumlah sel bakteri pengendali hama pada masa penyimpanan 3 minggu masih di atas 108 CFU/g.KeywordsBakteri; Bahan pembawa; Ekplorasi; Agens pengendali hama; Ulat grayak (Spodoptera litura)AbstractThe effort to control the Spodoptera litura at the farm level still used synthetic pesticides. This research aimed to explore potential bacteria as biological control of S. litura and do viability test of potential bacteria on compost and zeolite carrier. Soil potential bacteria had been isolated from three sources, including rhizosphere soil samples (rice, oil palm, eggplant, corn), rotten fruit samples (cocoa, oil palm, water), and insect samples (fireworms, locusts, dung beetles, butterflies) taken from Dramaga area with the purposive sampling method. Stages of laboratory study include isolation of bacterial isolates, pathogenicity tests, gram staining, colony morphology observation, toxicity test, biochemical test, and viability test. Two strains that have potential as biocontrol agents with a high ability to kill pests in the second stage of toxicity testing are IRJ 10 (90% mortality rate) and ISU 4 (100% mortality rate). Both of these isolates are members of the genus Bacillus. The highest number of viability was found in zeolite carriers. The number of bacterial cells in the three-week storage period is still above 108 CFU/g.
ANIMATION MEDIA DEVELOPMENT BASED MULTIPLE REPRESENTATIONS ON MATERIAL FACTORS AFFECTING CHEMICAL EQUILIBRIUM amanda maria meirina; Noor Fadiawati; chansyanah diawati
Jurnal Pendidikan dan Pembelajaran Kimia Vol 1, No 2 (2012): Jurnal Pendidikan dan Pembelajaran Kimia
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.14 KB)

Abstract

This researchaims to developmentchemical equilibriumanimation mediabased multiple representation. The researchbeginsby analyzingthe needsatseveral high schools inthe city ofBandarLampungto determinethe need forthe developmentof learninganimationmedia.Furthermore, the authorsdo product developmentin the form ofanimationmediabased multiplerepresentations. The resulting product isthen validatedby an expertdevelopercontent and designto determine thesuitability ofthe content, readability and attractivenessofdesignmediadevelopedlearninganimation. To test thesuitabilityaspect ofcontent,readability anddesignkemerarikananimatedmediaare developed, thenvalidatedbyexperts.Based on thevalidation ofthesuitability ofthe materialaspects ofanimationmedia-based learning multiplerepesentasion materialfactorsaffectingchemical equilibriumrated excellentwithan averagescore of4.88. As for thevalidation ofthedesignaspects oflegibilityand attractivenessmediaanimationrated excellentwithan averagescore of4.73. To determine theresponsesof teachersandstudents towardslearningdevelopedmediaanimationdonelimited testingat a schoolin the town ofBandarLampung. Based onlimited testingthat has beendonecan be concludedthatlearninganimationmediacan enhancestudent interestandmayassist students inmasteringconceptsin particularon materialfactorsaffectingchemical equilibrium. keywords: animation media, chemical equilibrium, multiplerepresentations
The potential of exopolysaccharide-producing bacteria from rhizosphere of rubber plants for improving soil aggregate Nasrul Harahap; Dwi Andreas Santosa; Nuni Gofar
Journal of Degraded and Mining Lands Management Vol 5, No 3 (2018)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.583 KB) | DOI: 10.15243/jdmlm.2018.053.1275

Abstract

 This study aimed to examine the effect of bacteria found in the rhizosphere of rubber plants in producing exopolysaccharides to improve aggregate stability of sandy soils. Samples of soil have been taken in rhizosphere of rubber plants in West Kalimantan. Serial soil samples were diluted and cultured on ATCC no.14 medium to select potential bacteria to produce exopolysaccharides. Forty-five isolates of exopolysaccharide-producing bacteria isolated from the rhizosphere of rubber plants was inoculated on ATCC no.14 medium. Based on the observations of morphological colony of these isolates, most of them had similarities in colour and shape so that only ten different isolates were obtained based on the morphological colony. Ten isolates were re-grown on MacConcey medium. Three isolates formed thick or slimy mucus when cultured on MacConcey medium. Three isolates grown on the medium of ATCC 14 resulted in dry weight of exopolysaccharide (mg/mL) varying from 0.28 to 7.59 mg/mL with sucrose and glucose as carbon sources. The results of the molecular identification of the three isolates of Klebsiella sp. LW-13, Klebsiella pneumoniae strain DSM 30104 and Burkholderia anthina strain MYSP113 showed that Klebsiella sp. LW-13 and Burkholderia anthina strain MYSP113 with 2% organic matter increased soil aggregate stability from highly unstable (30.67%) to unstable (45.01-48.20%). This aligned with the results by scanning electron microscopy (SEM) on treated soil and without bacteria treatments.  
PENGOLAHAN LIMBAH TEKSTIL SISTEM KOMBINASI ANAEROBIK-AEROBIK MENGGUNAKAN BIOFILM BAKTERI KONSORSIUM DARI LUMPUR LIMBAH TEKSTIL I Dewa K. Sastrawidana; Bibiana W. Lay; Anas Miftah Fauzi; Dwi Andreas Santosa
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 3 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The objective of this research are to assess the eficiency of biodegradation of textile dyes by using bacteriaconsortium biofim on combined anaerobic-aerobic system and also to asses the possibility for applicating this technologyin textile dyeing industry. Textile wastewater that used in this research are azo dyes total concentration 200 mg/L which isconsist of remazol yellow, remazol red, remazol black and remazol blue For anaerobik condition step, by using bacteriaconsortium selected from sludge of textile wastewater plant of Mama & Leon consist of Aeromonas sp ML6., Aeromonassp. ML14, Aeromonas sp. ML24, Pseudomonas sp. ML8 and Flavobacterium Sp ML20 whereas aerobic condition step,using bacteria consortium selected from Badung river sludge consist of Vibrio sp. and Plesiomonas sp. Both bacteriaconsortium is immobilized separatively on volcanous stone for 3 days in each reactor before used to treatment of synteticdyeswater (SDW). Further more, immobilized bacteria consortiom on volcanous stone is determined by four plate countmethod.The result ot this research showed the eficiency process in decreasing color, TDS, TSS, COD and BOD was96,94%,75,73%, 68,03%, 97,68% dan 94,60% respectively. So, wastewater treatment by using combined anaerobicaerobicsystem is potential to applied on wastewater treatment in textile industry.
The Efficacy of Bioaugmentation on Remediating Oil Contaminated Sandy Beach Using Mesocosm Approach (Efikasi Tehnik Bioaugmentasi dalam Memulihkan Pantai Berpasir Tercemar Minyak Menggunakan Pendekatan Mesokosm) Yeti Darmayati; Harpasis S. Sanusi; Tri Prartono; Dwi Andreas Santosa; Ruyitno Ruyitno
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 3 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.012 KB) | DOI: 10.14710/ik.ijms.20.3.143-152

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Bioremediation is basically consists of two approaches, biostimulation and bioaugmentation. The efficacy of bioaugmentation for combating oil pollution in field application is still argued. The purpose of study was to evaluate the efficacy of bioaugmentation and to compare the affectivity of single strain and consortium application in remediating oil polluted sandy beach. Experimental study in a field has been conducted with two (2) treatments and one (1) control in three different plots. The treatmens were introduction of a single strain (Alcanivorax sp TE-9) and a consortium (Alcanivorax sp. TE-9, Pseudomonas balearica st 101 and RCO/B/08-015) cultures into oil contaminated sediment. The experiment in mesocosm approach was taken place in Cilacap coast. Arabian light crude oil was used in the concentration of 100.000 mg.kg-1 sediment. Changes of oil concentration, bacterial density and pore water quality have been monitored periodically for 3 months. The result showed that oil degradation percentage and bacterial growth in both treatments were higher than in control. After 3 months, the percentage of oil degradation experiment in control, single strain and formulated consortium treatments were observed at 60.4%, 74.5% and 73.5%. It proves that bioaugmentation tehnique can enhance significantly oil biodegradation in sandy beach. The applications of bacteria in single or consortium culture give no different impact on their affectivity for bioremediation in Cilacap sandy beach. By data extrapolation it can be predicted that both of treatments able to reduce remediation time from 210 days into 135–137 days. Bioaugmentation can be proposed as a good solution for finalizing oil removing in Cilacap sandy beach when oil spilled occurred in this environment. Keywords: Bioremediation, bioaugmentation, oil, sandy beach, Alcanivorax, mesocosm, Cilacap   Bioremediasi pada dasarnya terdiri dari dua pendekatan yaitu biostimulasi dan bioaugmentasi. Teknik bioaugmentasi  dalam menanggulangi pencemaran minyak di lapangan masih diperdebatkan efektivitasnya. Penelitian ini bertujuan untuk mengevaluasi efikasi tehnik bioaugmentasi serta membandingkan efektivitas kultur tunggal dan konsorsium  dalam memulihkan pantai berpasir tercemar minyak. Studi eksperimental di lapangan telah dilakukan dengan menggunakan dua perlakuan dan satu kontrol di tiga plot berbeda, Perlakuannya adalah penambahan bakteri kultur tunggal (Alcanivorax sp TE-9) dan bakteri konsorsium (Alcanivorax sp. TE-9, Pseudomonas balearica st 101 dan RCO/B/08-015) ke dalam sedimen yang tercemar minyak. Eksperimen dengan pendekatan mesokosm dilakukan di pantai Cilacap. Minyak mentah ringan Arabia dengan konsentrasi 100.000 mg.kg-1 sedimen digunakan sebagai bahan cemaran. Perubahan konsentrasi minyak, kepadatan bakteri dan parameter lingkungan diamati secara periodik selama 3 bulan percobaan. Hasil penelitian menunjukkan bahwa persentase degradasi minyak dan densitas bakteri di sedimen perlakuan lebih tinggi daripada kontrolnya. Setelah 3 bulan eksperimen, persentase degradasi minyak pada kontrol, perlakuan kultur tunggal dan konsorsium masing-masing teramati 60.4%, 74.5% and 73.5%. Hal ini membuktikan bahwa tehnik bioaugmentasi secara signifikan mampu meningkatkan biodegradasi minyak di pantai berpasir. Pemberian mikroba dalam bentuk kultur tunggal ataupun konsorsium mempunyai efektivitas yang tidak berbeda untuk proses bioremediasi di pantai berpasir Cilacap.  Dengan ekstrapolasi data dapat diduga bahwa kedua perlakuan bioaugmentasi ini mampu mempercepat waktu pemulihan lingkungan dari 210 hari ke 135-137 hari. Bioaugmentasi dapat diusulkan sebagai solusi yang cukup baik untuk menghilangkan minyak pada tahap akhir pembersihan pantai berpasir Cilacap, jika terjadi tumpahan minyak di lingkungan ini. Kata kunci: single strain, consortium, bioaugmentation, oil, bioremediation, sandy beach,  Alcanivorax, Cilacap
Co-Authors . Giyanto . Giyanto Abdul Hasyim Sodiq Abdul Munif Agus Purwito Aisamrotul Hasanah Aksarah Pas, Aris Amanda Kusuma Dewi Anas Miftah Fauzi Arum Asiyanti Suhastyo, Arum Asiyanti Arum Asriyanti Suhastyo Atang Sutandi Batara, Lily Noviani Bedah Rupaedah Bibiana W Lay Bibiana W Lay BIBIANA W LAY Chansyanah Diawati Dedeh H Arief Dedi Widayat Dian Hendrayanti Didiek Hadjar Goenadi Didy Sopandie Dwi Wulan Pujiriyani Endriatmo Soetarto F. Dian Lina Fahrizal Hazra Falih, Fairuz Annabil Fitriyani, Indri Hapsari Giyanto, Giyanto Gunawan Djajakirana Gustav Adolf Wattimena Hamim Hamim HANNA ARTUTI EKAMAWANTI Hari R Harlyadi Harpasis S. Sanusi Harpasis S. Sanusi Haryanti Haryanti Hazrah, Fahrizal Hudi, Firdaus Alam I Dewa Ketut Sastrawidana Ilyas Jamil Iman Rusmana Indriyani, Yohanna Anisa Iskandar Iskandar Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Iswandi Anas Chaniago, Iswandi Anas Ivanovich Agusta Juhari Juhari, Juhari Khoirunnisa, Nur Syafira Kukuh Murtilaksono Kurnia Dewi Sasmita Kusuma Handayani Laksmita Prima Santi Laksono Trisnantoro LILIK BUDIPRASETYO Maggy T Suhartono Memen Surahman Mieke Rochimi Setiawati Miftahudin Muchamad Yusron Muhammad Rivai Nasrul Harahap NISA RACHMANIA MUBARIK Noegroho Hadi Noor Fadiawati Nuchsin, Ruyitno NUNI GOFAR Nurfadila, Uci Nursaadah . RAHAYU WIDYASTUTI Rani Nur Fitriani Remaja Sitepu Rustam, Rustam Ruyitno Ruyitno S Siswanto Sanjaya, Wilhelmus Terang Arga Sari, Retno Purnama Siswanto siswanto Slamet Susanto Sri Wilarso Budi Sudarsono Sukmadewi, Desak Ketut Tristiana SULASTRI Suryo Wiyono Susiyanti . Syaiful Anwar T W DARMONO Titik Tri Wahyuni Tommy Kurniawan Tornando, Hengki Tri Prartono Trikoesoemaningtyas Tualar Slmarmata Umi Hidayati Umi Hidayati Untung Sudadi Wahono Sumaryono Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya YADI SETIADI Yeti Darmayati Yeti Darmayati Yohanna Anisa Indriyani YULIN LESTARI Yuni Lisafitri, Yuni Yuyun Sumarni Zulfarina Zulfarina Zulfarina,