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All Journal Sodality: Jurnal Sosiologi Pedesaan Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Sainteks Ecotrophic, Journal of Environmental Science ILMU KELAUTAN: Indonesian Journal of Marine Sciences Agrikultura Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Soils Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati KOMUNITAS: INTERNATIONAL JOURNAL OF INDONESIAN SOCIETY AND CULTURE Jurnal Hortikultura Jurnal Pendidikan dan Pembelajaran Kimia (JPPK) Journal of Degraded and Mining Lands Management Jurnal Agroekoteknologi Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) BERITA BIOLOGI Jurnal Penelitian Karet Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Jurnal Matematika Sains dan Teknologi SAINS TANAH - Journal of Soil Science and Agroclimatology Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Kimia Terapan Indonesia Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotech Journal of Tropical Crop Science BHUMI: Jurnal Agraria dan Pertanahan Menara Perkebunan JURNAL PANGAN Jurnal Tanah dan Iklim International Journal on Food, Agriculture and Natural Resources (IJFANRES) Makara Journal of Science

DWI ANDREAS SANTOSA

Department Of Soil Sciences And Land Resource, Faculty Of Agriculture, IPB University, IPB Darmaga Campus, Bogor 16680, Indonesia

Author-ID : 256195

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Social Sciences Veterinary Other

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Pengaruh pemberian inokulan Burkholderia cenocepacia dan bahan organik terhadap sifat fisik tanah berpasir Effect of the inoculation of Burkholderia cenocepacia and organic materials on physical properties of a sandy soil Laksmita Prima SANTI; . SUDARSONO; Didiek Hadjar GOENADI; Kukuh MURTILAKSONO; Dwi Andreas SANTOSA
E-Journal Menara Perkebunan Vol 78, No 1: Juni 2010
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractSoil aggregation is a dynamic and very important factor for the development of agricultural soil functions. Unstable soil aggregation in a sandy soil type is a limiting factor for plant growth. The aim of this study was to investigate theinfluence of a exopolysaccharide-producing bacterium and organic material on some physical properties of a sandy soil. A highly potential bacterium for exopolysaccharides production, i.e Burkholderia cenocepacia, was isolated froma sandy soil located at Kotawaringin Barat, Central Kalimantan. The isolated bacterium is capable on improving aggregate’s stability of a soil with about 60% sand fraction [medium sand fraction (MSF)]. Interaction between available water and electric conductivity with aggregate stability index has significant correlation in medium sand fraction inoculated by 109 CFU of B. cenocepacia suspension, i.e. r = 0.806* and r=0.966**, respectively. Organic materials and its combination with the addition of 108 CFU B. cenocepacia suspension treatments could increase the aggregate stability index but have no effects on bulk density and total pore space values in an artificial condition of medium sand fraction. AbstrakAgregasi tanah bersifat dinamis dan merupakan faktor penting untuk pengembangan fungsi tanah pertanian. Ketidakstabilan agregat tanah pada jenis tanah berpasir merupakan faktor pembatas untuk pertumbuhan tanaman.Penelitian ini bertujuan untuk mengetahui pengaruh eksopolisakarida bakteri dan bahan organik terhadap beberapa sifat fisik bahan tanah berpasir. Satu bakteri potensial penghasil eksopolisakarida yaitu Burkholderia cenocepacia telah berhasil diisolasi dari bahan tanah berpasir asal Kotawaringin Barat, Kalimantan Tengah. Bakteri ini dapat meningkatkan kemantapan agregat bahan tanah dengan kandungan fraksi pasir sekitar 60% [fraksi pasir sedang (FPS)]. Hubungan antara air tersedia dan konduktivitas elektrik terhadap indeks kemantapan agregat sangat nyata pada bahan tanah FPS yang diinokulasi dengan 109 CFU suspensi B. cenocepacia masing-masing r = 0,806* dan = 0,966**. Perlakuan bahan organik dan kombinasinya dengan penambahan 109CFU suspensi B. cenocepacia dapat meningkatkan indeks kemantapan agregat tetapi tidak mempengaruhi nilai kerapatan lindak dan total ruang pori di dalam fraksi pasir sedang.

Pengembangan penanda molekuler untuk deteksi Phytophthora palmivora pada tanaman kakao Development of molecular marker for the detection of Phytophthora palmivora in cacao T W DARMONO; Ilyas JAMIL; Dwi Andreas SANTOSA
E-Journal Menara Perkebunan Vol 74, No 2: Desember 2006
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Summary Pod rot is one of the most important diseases in cacao. This disease could be incited by Phytophthora palmivora, P. megakarya, P. capsici or P. citrophthora. The causal agent of pod rot disease in cacao in Indonesia is known to be P. palmivora. The success of pod rot disease management is partly depend on the success of efforts in reducing the quantity and quality of the disease inoculum above and below soil surface. Provision of molecular-based detection system would improve the accuracy of determination of these two parameters. The objective of this experiment was to develop a pair of primers that could be used to specifically amplify rDNA fragments of P. palmivora associated with pod rot disease in cacao. Design of these primers was made based on the DNA sequence of rDNA fragment amplified using a pair of universal primers ITS4/ITS5. Regions showing high degree of dissimilarity among species of Phytophthora and high degree of similarity within the same species of P. palmivora were determined through DNA alignment. Specific forward primer (DTF) 5¢-CTT AGT TGG GGG TCT CTT TC-3¢ and reverse primer (Ilyas1R) 5¢-GTT CAC CAA TCA TAC CAC C-3¢ were obtained. This pair of primers had been proven to specifically amplify only rDNA fragment, approximately 650 bp, of P. palmivora associated with pod rot disease and stem canker in cacao.Ringkasan Penyakit busuk buah merupakan salah satu penyakit terpenting pada tanaman kakao. Penyakit ini dapat disebabkan oleh Phytoph-thora palmivora, P. megakarya, P. capsici atau P. citrophthora. Di Indonesia busuk buah disebabkan oleh P. palmivora. Keberhasilan pengendalian penyakit busuk buah salah satunya tergantung kepada keberhasilan penekanan kuantitas dan kualitas inokulum baik yang berada di atas maupun di bawah permukaan tanah. Tersedianya perangkat deteksi molekuler akan sangat membantu dalam upaya penetapan kedua parameter ter-sebut. Penelitian ini bertujuan untuk mengem-bangkan satu pasang primer yang secara spesifik mampu mengamplifikasi hanya fragmen rDNA P. palmivora yang berkaitan dengan busuk buah kakao. Desain primer dilakukan dengan mengacu kepada sekuen rDNA yang diamplifikasi dengan pasangan primer universal ITS4/ITS5. Daerah yang menunjukkan urutan basa dengan tingkat keragaman yang tinggi antar spesies Phytoph-thora dan yang menunjukkan tingkat kesamaan tinggi dalam satu spesies P. palmivora yang sama ditelusuri melalui penjajaran DNA. Hasil desain primer diperoleh primer forward (DTF) 5¢-CTT AGT TGG GGG TCT CTT TC-3¢ dan reverse (Ilyas1R) 5¢-GTT CAC CAA TCA TAC CAC C-3¢. Pasangan primer DTF dan Ilyas1R ini hanya mampu mengamplifikasi fragmen rDNA berukuran 650 bp dari P. palmivora penyebab penyakit buah dan kanker batang kakao.

Aplikasi Konsorsium Mikrob Filosfer dan Rizosfer Untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Padi Application of Phyllosphere and Rhizosphere Microbial Consortium to Improve Rice Growth and Production Aris Aksarah Pas; Didy Sopandie; Trikoesoemaningtyas Trikoesoemaningtyas; Dwi Andreas Santosa Santosa
JURNAL PANGAN Vol. 24 No. 1 (2015): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v24i1.39

Pendekatan secara biologi, memanfaatkan konsorsium mikrob filosfer dan mikrob rizosfer merupakan langkah alternatif mengurangi dampak negatif penggunaan pupuk sintetik, untuk mencapai produksi padi yang tinggi. Penelitian ini bertujuan untuk mempelajari peran konsorsium mikrob filosfer dan mikrob rizosfer terhadap pertumbuhan dan hasil tanaman padi. Penelitian dilakukan mulai bulan Januari hingga Mei 2014, di Green House Indonesian Centre For Biodiversity and Biotecnology, Bogor. Dalam penelitian ini digunakan konsorsium mikrob terbaik hasil seleksi, yaitu konsorsium mikrob filosfer Fm48 dari daun tumbuhan Emmerrilia ovalis Miq Dandy dan konsorsium mikrob rizosfer R15 dari rizosfer tumbuhan Physalis angulata L. Penelitian menggunakan Rancangan Acak Kelompok (RAK) satu faktor, yang terdiri atas tiga perlakuan, yaitu : pemberian pupuk N sintetik setengah dosis anjuran yaitu 30 N/ha, pemberian pupuk N sintetik sesuai dosis anjuran, yaitu 60 kg N/ha dan pemberian kombinasi konsorsium mikrob filosfer Fm48 dan mikrob rizosfer R15 dengan diberi pupuk N sintetik setengah dosis anjuran. Hasil penelitian menunjukkan bahwa, aplikasi kombinasi konsorsium mikrob dengan pemberian pupuk N sintetik setengah dosis anjuran, meningkatkan jumlah anakan, bobot kering tanaman, jumlah anakan produktif dan bobot segar malai setara dengan pemberian pupuk N sintetik sesuai dosis anjuran. Biological approach, by utilizing phyllosphere and rhizosphere microbial consortium, offers n alternative method to avoid the negative impact of synthetic fertilizer to the environment to achieve higher rice production. This research aims to study the role of phyllosphere and rhizosphere microbial consortium on the growth and yield of rice plants. The study was conducted from January to May 2014 in Green House Indonesian Centre For Biodiversity and Biotechnology, Bogor. Microbial consortium of phyllosphere Fm48 from plant leaves Emmerrilia ovalis Miq Dandy and microbial consortium of rhizosphere R15 from plant rhizosphere Physalis angulata L. are selected for this study. This research is designed in randomized block design with one factor, which consists of three treatments, namely: half recommended dose of synthetic N fertilizer (30 kg N/ha), recommended dose of Synthetic N fertilizer (60 kg N/ha), and a combination of microbial consortium of phyllosphere and rhizosphere microbes plus half recommended dose of synthetic N fertilizer. The results show that the applications of microbial consortium combined with half of recommended dose of synthetic N fertilizer, increases the number of tillers, plant dry weight, number of productive tillers, and panicles fresh weight equivalent to the use of recommended dosage of synthetic N fertilizer.

Molecular identification of isolates from local microorganisms as potential biofertilizer Abdul Hasyim Sodiq; Mieke Rochimi Setiawati; Dwi Andreas Santosa; Dedi Widayat
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 18, No 2 (2021): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v18i2.44476

Local microorganisms (MOL) are liquid fertilizers commonly used by farmers to help increase crop production. Beneficial microbes in MOL need to characterize their interactions and ability to produce growth drive compounds. The purpose of this research is to identify the superior microbial isolates from MOL made by farmers from Cibodas Lembang Bandung, Indonesia that can produce phytohormones as biofertilizers. The results of the microbial selection of MOL derived from three best microbes are 1A-2 NFB, 4A-1 NFB, and 4B-1 NFB with the ability to produce auxin, i.e., 19.41 ppm, 17.18 ppm, and 10.59 ppm, respectively. The compatibility test between the three isolates showed negative results so that it was possible to apply three microbes as a consortium. The results of a molecular identification with a 16S rRNA analysis indicate strain microbe 1A-2 NFB: Bacillus cereus (99.88% homology), 4A-1 NFB: Bacillus cereus (99.76% homology), and 4B-1 NFB: Lysinibacillus sp. (99.88% homology).

TEKNIK APLIKASI KONSORSIUM BAKTERI ENDOFITIK PENAMBAT N2 ASAL TUMBUHAN EKOSISTEM AIR HITAM KALIMANTAN TENGAH DALAM MENINGKATKAN PERTUMBUHAN DAN PENAMBATAN N2 TANAMAN PADI GOGO Mieke R Setiawati; Tualar Slmarmata; Yuyun Sumarni; Dedeh H Arief; Dwi Andreas Santosa; Hari R Harlyadi
Jurnal Kimia Terapan Indonesia Vol 12, No 1 (2010)
Publisher : Research Center for Chemistry - LIPI

This study was intended to derive an application technique of bacterial consortium obtained from plantation black water ecosystem of CentraKalimantan, and therefore to increase the nitrogen fixation and growth of the upland rice. The experiments were conducted in the Biology and Biotechnology Laboratory, Faculty of Agriculture, PadjadjaranUniversity, Jatinangor-West Java. The preliminary selection of inoculum source from the plantation ecosystem at black water ecosystem of Central Kalimantan produced 12 consortia of the endophytic bacterium that increase the growth of upland rice. Among of the 12 consortia, 2 consortia (did contribute nitrogen fixation consistently and so increase the growth of upland rice. The two consortia were a bacterial consortium (H) isolatedfrom the leaves of Waru plant (Dillenia pulchella) and a bacterial consortium (I) isolatedfrom Gemor plant (Alceodaphde sp.). Threefactorial of Complete-Random Design and triplo experiment was selected for the experimentaldesign of this study. First factor was consortium of endophytic nitrogen fixation bacterial with two stages (i1 = consortium H and i2 = consortium I). The second factor was the technique of endophytic nitrogen fixationbacteria application with two stages (aI = rice seed soaked in the bacterial suspension for 24 hours and a2= 1 MST old rice plant sprayed the bacterial suspension). The third factor was turbidity (population) of endophytic nitrogen fixation bacterial inoculum with 4 stages (Po=no inoculum control, PI=107 CFU.mtI, P2=109 CFU/mtI, P3= 1011CFU/ml). The CFU is a Colony-Forming Unit indicating microbes in aform of colony, initially, grew from single cell. The results of this experiment showed that the application technique of endophytic nitrogen fixation bacteria by soaking the upland rice seed in bacterial suspension containing 1011 CFU.mrI for 24 hours wasable to increase the growth and nitrogen fixation of the upland rice plant. Dry weight of the plant inoculated with consortia Hand I was respectively 0.084 and 0.097g while the nitrogenase activity of the upland rice plantinoculated with consortia Hand I was 254.0 and 225.0 nmol g"1 DWhou,-I, respectively. With Microbact 24 E (12E/12A + 12B) and API-20 NE methods, member of endophytic nitrogen fixation bacteriafrom consortium H was indentified as Klebsiella pneumoniae, Pseudomonas stutzeri, Brevundimonas vesicularis, and Burkholderia cepacia while from consortium I was Klebsiella pneumoniae, Pseudomonas fluorescens, Burkholderia cepacia,and Acinetobacter baumanii.Kata Kunci : Endophytic bacteria.Nitrogen, Plantation blackwaterecosystem, Uplandriceplant.

The Abundance of Nitrogen Fixing, Nitrifying, Denitrifying and Ammonifying Bacteria in the Soil of Tropical Rainforests and Oil Palm Plantations in Jambi Zulfarina,; Rusmana, Iman; Mubarik, Nisa Rachmania; Santosa, Dwi Andreas
Makara Journal of Science Vol. 21, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Alterations in the use of land for oil palm plantations can change the domination and activity of soil bacteria. More specifically, alteration in soil microbial communities can directly affect soil ecosystem functioning, particularly with respect to carbon and nitrogen cycles. Nitrogen can be a limiting nutrient, and the availability of nitrogen in the soil environment becomes a major factor in controlling the production of biomass. This research project aimed at studying the abundance of nitrogen-fixing, nitrogen-oxidizing, nitrogen-reducing, and ammonifying bacteria based on their functional genes in the tropical rain forests of Taman Nasional Bukit Duabelas (TNBD) and the oil palm plantations in Sarolangun Jambi. Samples were collected in November 2015. Soil sampling was performed randomly at three points representing each area of the tropical rainforests of TNBD and the seven- to eight-year-old oil palm plantations. Soil samples were collected using a soil sample core from 0–15 cm below the surface with depth strata of 0–5 cm, 5–10 cm, and 10–15 cm. Composite assessment was conducted on samples from each point corresponding to each respective depth strata. Soil samples were stored at -20ºC prior to testing. Microbial abundance was measured using the most probable number (MPN) method. The abundance of microbes that play a role in nitrogen metabolism between strata of 5–10 cm and 10–15 cm does not appear to be different. The highest abundance of microbes in oil palm plantation land in Jambi was found in samples with nitrifying bacteria, later followed by denitrifying, nitrogen-fixing, and ammonifying bacteria. Ultimately, it was found that microbial abundance in oil palm plantations was higher than the corresponding rates in samples from tropical rainforests.

Efektivitas Pupuk Hayati Cair pada Tanaman Padi Sawah (Oryza Sativa) Serta Analisis Usaha Taninya: The Effectiveness of Liquid Bio-Fertilizer on Rice Plants (Oryza sativa) and the Analysis of Farming Business Fahrizal Hazra; Dwi Andreas Santosa
Jurnal Ilmu Tanah dan Lingkungan Vol 24 No 2 (2022): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.24.2.39-46

Petani di Indonesia mulai memahami pentingnya pupuk hayati dengan standar kualitas dan efektivitas yang dipersyaratkan oleh Kementerian Pertanian RI. Sehingga banyak pupuk hayati yang beredar di pasaran perlu dilakukan penelitian untuk menguji kualitas dan efektivitasnya. Tujuan penelitian ini adalah untuk menguji kualitas dan efektivitas, serta analisis usahatani pupuk hayati cair pada padi sawah (Oriza sativa). Perlakuan disusun dalam 5 taraf pemupukan yaitu: tanpa pemupukan atau kontrol (P0), 1,00 dosis NPK (P1), 1,00 dosis NPK + 1,00 dosis pupuk hayati (P2), 0,75 dosis NPK + 1,00 dosis pupuk hayati (P3), 0,50 dosis NPK + 1,00 dosis pupuk hayati (P4). Percobaan dilakukan dengan 4 ulangan sehingga terdapat 20 satuan percobaan. Setiap satuan percobaan merupakan sebidang tanah dengan luas 25 m2. Hasil penelitian menunjukkan bahwa uji mutu memenuhi kriteria Permentan No. 1 Tahun 2019, serta penerapan pupuk hayati di lapangan secara umum menghasilkan pertumbuhan dan hasil tanaman padi sawah yang sama dengan pembanding, dan lebih baik dari perlakuan kontrol. Perlakuan 0,75 dosis NPK + 1,00 dosis pupuk hayati merupakan perlakuan yang paling efektif dan menguntungkan secara agronomis dengan nilai RAE 100%, dan secara ekonomis dengan nilai R/C 1,83.

Kontribusi Sifat Biokimia Tanah sebagai Determinan Produktivitas Lahan Perkebunan Nanas (Ananas comosus [L.] Merr.) di Kabupaten Lampung Tengah Kusuma handayani; Nisa Rachmania Mubarik; Atang Sutandi; Dwi Andreas Santosa; Untung Sudadi
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 12 No 4 (2022): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.12.4.729-739

One of the management challenges in pineapple cultivation at plantation scale is the still occurrence of disparity in land productivity across the cultivated area although the cropping practices have been implemented for many years. In the case of a pineapple plantation in Central Lampung Regency, contribution of soil biochemical properties in terms of various soil enzyme activities as a determinant factor of land productivity has never been elaborated. This research was aimed to study the relationships among biochemical and other soil properties with land productivity or pineapple yield at plantation scale. Rhizosphere soil-composite samples were taken purposively from 4 stations at the largest Indonesian pineapple plantation representing blocks with high and low yield and growth at vegetative and generative phase. Relationships amongst the studied parameters were evaluated using PCA and linear multiple regression analysis. The results showed significant contributions of the rhizosphere soil properties on the pineapple yield according to equation: Yield = 64.895 – 6.546 PCA1 +13.057 PCA2 – 7.722 PCA4 (R2= 0.612), where PCA1 consisted of soil available-P, available-K, and CEC; PCA2 was of soil base saturation, total microbe population, enzyme activities of cellulase and invertase, while PCA4 was of soil organic C, silt fraction, and phosphatase activities.

Deagrarianization and Local Food Security Strategy for Peasant Communities in Rural Central Java Pujiriyani, Dwi Wulan; Soetarto, Endriatmo; Santosa, Dwi Andreas; Agusta, Ivanovich
Komunitas Vol 11, No 2 (2019): September 2019
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/komunitas.v11i2.20209

Food security is one of the important issues in the study of deagrarianization. In macro level, deagrarianization can weaken food security which is indicated by the ability of food production which continues to decline due to the structural transformation of the economy from agriculture to non-agriculture. The purpose of this study is to determine food security at the community level when the symptoms of macro deagrarianization have occurred. This research is conducted by qualitative methods. An ethnosociological approach is used by combining community case study methods and ethnographic methods. The results show that food security at the community level is still well-maintained. Deagrarianization has not diminished the ability of the community to meet their food needs. Communities have internal mechanisms that secure their food sufficiency through food strategies and non-food strategies. The food strategy is carried out through saving yields for grain stores (family food barns) and reduce the amount of consumption. Meanwhile the non-food strategy is carried out through diversification of livelihoods by relying on available extractive landscapes. Swamps (balongan) are used as a food source that provides various types of local fish, crabs and snails as a source of protein for family consumption and also for sale to local collectors.

Harvesting Bioelectricity from Microbial Fuel Cells (MFCs) Powered by Electroactive Microbes Yohanna Anisa Indriyani; Iman Rusmana; Syaiful Anwar; Gunawan Djajakirana; Dwi Andreas Santosa
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 12, No 3 (2023): September 2023
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v12i3.583-596

The application of microbial fuel cells is still facing some challenges due to its low power output and high internal resistance. It is desirable to obtain a stable and consistent power output from an MFC to support practical real-world applications. Five electroactive bacteria (isolate LGf1, LGf11, LGf15, LGf20, and LGf22) isolated from the sediment of Waduk Saguling were exploited as the potential anodic biocatalyst for MFC, and the performance of these MFCs were studied in terms of voltage generation (open and close circuit), power density and the losses (polarization technique), and efficiencies (coulombic and energy). MFC biocatalyst by isolate LGf11 performed the best electrochemical performances, including highest OCV (open circuit voltage) value (804 mV) and power output (0.043 W/m2), lowest ohmic resistance (475 Ω), and highest coulombic efficiency (75.79%) and energy efficiency (88.36%) among all anodic biocatalysts. Nevertheless, all the five isolates were potential to be exploited as active biocatalyst for MFC due to their high OCV values and the stability of voltage generations, both in open circuit and close circuit mode. The development of system configuration and the use of more suitable substrate for different electroactive microbes in order to harvest more power output was recommended for further study. Utilization of these potential microbes for other applications in MFC (such as wastewater treatment etc.) was also suggested for further research. Keywords: Bio-electrochemical system, Biofuel, Efficiency, Electro-microbiology, Power output

Co-Authors . Giyanto . Giyanto Abdul Hasyim Sodiq Abdul Munif Agus Purwito Aisamrotul Hasanah Aksarah Pas, Aris Amanda Kusuma Dewi Anas Miftah Fauzi Arum Asiyanti Suhastyo, Arum Asiyanti Arum Asriyanti Suhastyo Atang Sutandi Batara, Lily Noviani Bedah Rupaedah Bibiana W Lay Bibiana W Lay BIBIANA W LAY Chansyanah Diawati Dedeh H Arief Dedi Widayat Dian Hendrayanti Didiek Hadjar Goenadi Didy Sopandie Dwi Wulan Pujiriyani Endriatmo Soetarto F. Dian Lina Fahrizal Hazra Falih, Fairuz Annabil Fitriyani, Indri Hapsari Giyanto, Giyanto Gunawan Djajakirana Gustav Adolf Wattimena Hamim Hamim HANNA ARTUTI EKAMAWANTI Hari R Harlyadi Harpasis S. Sanusi Harpasis S. Sanusi Haryanti Haryanti Hazrah, Fahrizal Hudi, Firdaus Alam I Dewa Ketut Sastrawidana Ilyas Jamil Iman Rusmana Indriyani, Yohanna Anisa Iskandar Iskandar Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Iswandi Anas Chaniago, Iswandi Anas Ivanovich Agusta Juhari Juhari, Juhari Khoirunnisa, Nur Syafira Kukuh Murtilaksono Kurnia Dewi Sasmita Kusuma Handayani Laksmita Prima Santi Laksono Trisnantoro LILIK BUDIPRASETYO Maggy T Suhartono Memen Surahman Mieke Rochimi Setiawati Miftahudin Muchamad Yusron Muhammad Rivai Nasrul Harahap NISA RACHMANIA MUBARIK Noegroho Hadi Noor Fadiawati Nuchsin, Ruyitno NUNI GOFAR Nurfadila, Uci Nursaadah . RAHAYU WIDYASTUTI Rani Nur Fitriani Remaja Sitepu Rustam, Rustam Ruyitno Ruyitno S Siswanto Sanjaya, Wilhelmus Terang Arga Sari, Retno Purnama Siswanto siswanto Slamet Susanto Sri Wilarso Budi Sudarsono Sukmadewi, Desak Ketut Tristiana SULASTRI Suryo Wiyono Susiyanti . Syaiful Anwar T W DARMONO Titik Tri Wahyuni Tommy Kurniawan Tornando, Hengki Tri Prartono Trikoesoemaningtyas Tualar Slmarmata Umi Hidayati Umi Hidayati Untung Sudadi Wahono Sumaryono Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya YADI SETIADI Yeti Darmayati Yeti Darmayati Yohanna Anisa Indriyani YULIN LESTARI Yuni Lisafitri, Yuni Yuyun Sumarni Zulfarina Zulfarina Zulfarina,

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