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Coupling Indonesian indigenous Citrobacter freundii and Chlorella pyrenoidosa strain on the anode of microbial fuel cell with various substrates IRFAN ANWAR FAUZAN; ANJA MERYANDINI; RONI RIDWAN; RUSLI FIDRIYANTO; NI WAYAN SRI AGUSTINI; DWI ANDREAS SANTOSA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 5 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230527

Abstract

Abstract. Fauzan IA, Meryandini A, Ridwan R, Fidriyanto R, Agustini NWS, Santosa DA. 2022. Coupling Indonesian indigenous Citrobacter freundii and Chlorella pyrenoidosa strain on the anode of microbial fuel cell with various substrates. Biodiversitas 23: 2471-2481. Microorganism plays a crucial role in the development of MFC systems. Indigenous to Indonesia, Citrobacter freundii GBH253 is a potential exoelectrogenic bacterium that could be developed into an MFC system. Coupling C. freundii GBH253 with potentially electricity-producing microalgae indigenous to Indonesia, such as Chlorella pyrenoidosa INK, in the anode of an MFC, could result in a more stable and higher electricity output. This study used C. freundii GBH253 and C. pyrenoidosa INK to produce electricity in various substrates. This research was conducted using a Factorial Randomized Block Design and Tukey’s test to determine significant differences between treatments. The result shows that electricity was generated in all treatments. The Bacterium-microalgae combination in acetate substrate can generate power density up to 211,97 mW m-2 and is the most stable compared to others. Bacterium dominates the electricity production in this combination, but the microalgae also play a role in producing electricity and increasing Chemical Oxygen Demand. The pH value of all treatments was higher than 7. Volatile Fatty Acids, like acetate and phenol, were produced in all treatments, whereas butyric acid and propionic acid were produced in several treatments. The Pearson correlation showed that some VFAs are highly correlated with power density.
Selection for representative environment to identify high yielding and sweetness levels of sweet potato (Ipomoea batatas L.) in Indonesia ESO SOLIHIN; DWI ANDREAS SANTOSA; BUDI NUGROHO; PURWONO PURWONO; RIJA SUDIRJA; HARIS MAULANA; NADIA NURANIYA KAMALUDDIN; AGUNG KARUNIAWAN; SYAIFUL ANWAR
Biodiversitas Journal of Biological Diversity Vol. 25 No. 1 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250145

Abstract

Abstract. Solihin E, Santosa DA, Nugroho B, Purwono, Sudirja R, Maulana H, Kamaluddin NN, Karuniawan A, Anwar S. 2024. Selection for representative environment to identify high yield and sweetness levels of sweet potato (Ipomoea batatas) in Indonesia. Biodiversitas 25: 386-391. Sweet potato is one of the important and commercial agricultural commodities in the world. Yield and sweetness levels are important traits that can influence consumer preferences. Environment is an important factor in the development of sweet potatoes. A suitable environment will provide high yields and good quality. The objectives of this study were to examine the significance and relevance of sweet potato yield and sweetness levels in two locations (Sumedang and Bandung District) and to select representative environments for sweet potato yield and sweetness levels. The research was conducted in Sumedang and Bandung districts on lowland paddy field and dry land. The field experiment used a factorial randomized completed block design with three repetitions. The significance and relevance of sweet potato yield and sweetness levels was analysis using T-test. Representative environments were identified by GGE biplot. The results showed that the Bandung and Sumedang environments showed significant differences in yield but not in sweetness. The Bandung paddy field is the most representative environment for yield, while the Sumedang dry field and Bandung dry field are the most representative environments for sweetness. The results of this research can be used by researchers, farmers and industrial users in the sweet potato development program, especially the yield and yield quality.
Isolation and identification of heterotrophic bacteria with methane oxidation potential from various ecosystems as biological agents for reducing methane emissions ERNI WIDIASTUTI; DWI ANDREAS SANTOSA; SYAIFUL ANWAR
Biodiversitas Journal of Biological Diversity Vol. 27 No. 4 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270423

Abstract

Abstract. Widiastuti E, Santosa DA, Anwar S. 2026. Isolation and identification of heterotrophic bacteria with methane oxidation potential from various ecosystems as biological agents for reducing methane emissions. Biodiversitas 27 (4): d270423. https://doi.org/10.13057/biodiv/d270423. Methane is the second-largest greenhouse gas after carbon dioxide, contributing to global warming. Methane-oxidizing bacteria can help reduce methane emissions. However, the availability of methane-oxidizing bacteria isolated from various Indonesian ecosystems remains very limited, leaving a knowledge gap regarding their diversity and functional potential. This study aimed to isolate and identify bacteria from the swamp, river, and cattle manure ecosystems. Sediment samples were collected at 1-10 cm depth from five points in each ecosystem. Bacteria were isolated using a nitrate mineral salt medium supplemented with methane gas as a carbon source during incubation. The bacteria obtained were subjected to further analysis, namely morphological observation, pathogenicity testing through hypersensitivity and hemolysis analysis, indirect testing of Particulate Methane Monooxygenase (pMMO) and Soluble Methane Monooxygenase (sMMO) enzyme activity. Methane oxidation activity was quantified in three replicates for each isolate using gas chromatography. Molecular identification involved Polymerase Chain Reaction (PCR) amplifying the 16S rRNA gene. There are nine potential bacterial isolates with diverse morphological forms. In vitro tests show that all bacteria are non-pathogenic, with methanol production indirectly indicating pMMO enzyme activity, showing an average concentration of 1.80 mM, while sMMO activity is negative. The three best bacteria, Rw3, Rw4, and Sn1, had potential CH4 oxidation capabilities of 66.61, 62.39, and 62.55%, respectively, under laboratory conditions. The three bacteria were identified as Achromobacter sp., Exiguobacterium sp., and Burkholderia sp., with similarity values of 97.41%, 99.88%, and 98.85%, respectively. Although these three genera are not classified as methanotrophs, they exhibit potential methane-oxidizing activity under laboratory conditions. Overall, this study highlights the potential role of diverse heterotrophic bacterial genera in oxidizing methane, making them relevant biological candidates in the methane cycle.
Co-Authors , Giyanto . Giyanto Abdul Hasyim Sodiq Abdul Munif AGUNG KARUNIAWAN Agus Purwito Aisamrotul Hasanah Aksarah Pas, Aris Amanda Kusuma Dewi Anas Miftah Fauzi Anja Meryandini Arum Asiyanti Suhastyo, Arum Asiyanti Arum Asriyanti Suhastyo Atang Sutandi Batara, Lily Noviani Bedah Rupaedah Bibiana W Lay Bibiana W Lay BIBIANA W LAY BUDI NUGROHO Chansyanah Diawati Dedeh H Arief Dedi Widayat Dian Hendrayanti DIAN HENDRAYANTI Didiek Hadjar Goenadi Didy Sopandie Dwi Wulan Pujiriyani Endriatmo Soetarto ERNI WIDIASTUTI ESO SOLIHIN Ester Raisa K. Lase F. Dian Lina Fahrizal Hazra Fahrizal Hazra Falih, Fairuz Annabil Firdaus Alam Hudi Fitriyani, Indri Hapsari Giyanto Giyanto Giyanto Giyanto, Giyanto Gunawan Djajakirana GUNAWAN DJAJAKIRANA Gustav Adolf Wattimena HADI WISA NUGRAHA HAMIM Hamim Hamim HANNA ARTUTI EKAMAWANTI Hari R Harlyadi HARIS MAULANA Harpasis S. Sanusi Harpasis S. Sanusi Haryanti Haryanti Hazrah, Fahrizal Hengki Tornando I Dewa Ketut Sastrawidana Ilyas Jamil IMAM RUSMANA Iman Rusmana Indriyani, Yohanna Anisa IRFAN ANWAR FAUZAN Iskandar Iskandar Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Iswandi Anas Chaniago, Iswandi Anas Ivanovich Agusta Juhari Juhari, Juhari Khoirunnisa, Nur Syafira Kukuh Murtilaksono Kurnia Dewi Sasmita Kusuma Handayani Laksmita Prima Santi Laksono Trisnantoro Lilik Budi Prasetyo Maggy T Suhartono Memen Surahman Mieke Rochimi Setiawati Miftahudin Muchamad Yusron Muhammad Rivai NADIA NURANIYA KAMALUDDIN Nasrul Harahap NI WAYAN SRI AGUSTINI NISA RACHMANIA MUBARIK Noegroho Hadi Noor Fadiawati NUNI GOFAR Nur Syafira Khoirunhisa Nur Syafira Khoirunnisa Nurfadila, Uci Nursaadah . PURWONO PURWONO RAHAYU WIDYASTUTI Rani Nur Fitriani Remaja Sitepu RIJA SUDIRJA RONI RIDWAN RUSLI FIDRIYANTO Rustam, Rustam Ruyitno Nuchsin Ruyitno Ruyitno S Siswanto Sanjaya, Wilhelmus Terang Arga Sari, Retno Purnama Siswanto siswanto Slamet Susanto Sri Ismiani Sri Wilarso Budi Sudarsono Sukmadewi, Desak Ketut Tristiana SULASTRI Suryo Wiyono Susiyanti . SYAIFUL ANWAR SYAIFUL ANWAR Syaiful Anwar SYAIFUL ANWAR SYAIFUL ANWAR T W DARMONO Titik Tri Wahyuni Tommy Kurniawan Tri Prartono Trikoesoemaningtyas Tualar Slmarmata Umi Hidayati Umi Hidayati Untung Sudadi Wahono Sumaryono Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya YADI SETIADI Yeti Darmayati Yeti Darmayati Yohanna Anisa Indriyani YULIN LESTARI Yuni Lisafitri, Yuni Yuyun Sumarni Zulfarina Zulfarina Zulfarina,