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Journal : Microbiology Indonesia

Molecular and Physiological Characterization of Copper-Resistant Bacteria Isolated from Activated Sludge in an Industrial Wastewater Treatment Plant in Rungkut-Surabaya, Indonesia WAHYU IRAWATI; TRIWIBOWO YUWONO; JOEDORO SOEDARSONO; HARI HARTIKO
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (850.788 KB) | DOI: 10.5454/mi.6.3.3

Abstract

Copper resistant bacteria can be isolated from environments where copper levels are abundant from mining, industrial, or agricultural activities. The aim of this work was to study the molecular and physiologicalcharacteristics of indigenous copper resistant bacteria isolated from activated sludge in an industrial wastewatertreatment plant in Surabaya, Indonesia. The bacterial isolates were designated as strains IrC1, IrC2, and IrC4. Phylogenetic analysis based on 16S rDNA sequence analysis identified isolates IrC1, IrC2, and IrC4 as Acinetobacter oleivorans (98.41% similarity), Acinetobacter pitii (97.22% similarity), and Cupriavidus pauculus (96.99 similarity), respectively. The addition of 5 mM of CuSO4 in the medium affected morphological 4 appearance of all isolates to green and undulate margin might be due to the survival mechanism of bacteria by absorbing the copper. This studies indicated that copper resistance mechanism of all isolates was facilitated through the bioaccumulation of copper inside the cell, especially on the membrane fraction and inside the cytoplasm, albeit at a limited amount. It was observed that isolates IrC1, IrC2, and IrC4 were capable of accumulating 137.23 , 364.66 , and 272.07 mg L-1 of copper, respectively from the medium containing 8 mM CuSO4. The capability of isolates IrC1, IrC2, and IrC4 to accumulate copper can be exploited in bioremediation 4 process for removing copper from industrial sewage.
Heavy Metals Biosorption by Copper Resistant Bacteria of Acinetobacter sp. IrC2 WAHYU IRAWATI; ADOLF JN PARHUSIP; NIDA SOPIAH
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2817.263 KB) | DOI: 10.5454/mi.9.4.4

Abstract

 Heavy metal pollution is a serious problem because it cannot be degraded by natural processes and persist in soil, water, and sediment. Many indigenous microorganisms isolated from heavy metal contaminated sites had tolerance to heavy metals toxicity and could be used for bioremediation agent because of its capability to biosorb heavy metals. The aim of this research was to study the potency of copper resistant bacteria Acinetobacter sp. IrC2 as a biosorbent of heavy metals. Biosorption was determined by measuring the heavy metals concentration on growing medium by using atomic absorption spectrophotometer. The research showed that Acinetobacter sp. IrC2 was capable of growing in medium containing each of 2 mM of copper, zinc, lead, cadmium, and the mixture of those heavy metals. The addition of copper and lead in the medium changed morphological appearance of colonies to green and brown, respectively, suggesting that the survival mechanism of the isolate was by biosorbing copper or lead inside the cells. The percentage of heavy metals biosorption efficiency using live cells of Acinetobacter sp.IrC2 were up to 64.31% of copper, 24.73% of zinc, 62.79% of lead, and 11.56% of cadmium. Acinetobacter sp. IrC2 also reduced copper, lead, and cadmium concentration up to 24.30, 75.93, and 16.38%, respectively, in medium supplemented with 1 mM of the mixture of these heavy metals. The findings of this study indicated that Acinetobacter sp. IrC2 was a promising bacterium for removal of heavy metals.
Co-Authors . PATRICIA Aaron Hasthosaputro ABYATAR HUGO BASKORO Alvionita Thana Alya Leonita Lamonta Ambarita, Polin P. Ambarita, Polin Parulian Andara Frida Sheilaliany Daely Angelica, Apriliana Anggun Anggun Aninditya Sri Nugraheni Argoby Simanullang Argorby Simanullang Artha, David Dharmawan Ashiong P Munthe Ashiong Parheheon Munthe Ayustin, Yohana Delvia Bellen Mouretta Bermuli, Jessica Elfani Bora, Merliana Ina Cantika Dela Kurnia Zendrato Charles Immanuel Chelvieana A. Christanti Christeven Mevotema Christian, Grace Purnamasari Christian, Salomo Clement Khrisman Laia Clement Khrisman Laia Daely, Andara Frida Sheilaliany Damai Yanti Manalu David L Gultom Debora V. Widyanti Delfania Apang Madao Deokward F. Ruga Desi L. Sihombing Destya Waty Silalahi, Destya Waty Dipakalyano Dipakalyano Dipakalyano, Dipakalyano Djoko Prasetyo Dominica Feni Valentina Dr. Adolf J.N. Parhusip Dwany, Naomi Dwi N. Susilowati Egilia Lucky Elisabeth Tita Ellen Nur Endah Pangesti Elysabeth Simatupang Eunike Bunga Marvella Eunike Bunga Marvella Eunike Bunga Marvella Fernando Christofer Fernando Christofer Fernando Corneles Moniharapon Fiska Ivana Pratami Putri Tokede Floreta D. Icasari Frichilya Intan Lawa Padang Gaol, Riris Lumban Geoffrey Darrien Fideli Georgine Gladis Paula Sulardi Gim Mi Kyong Giri, Mutiara Yaemeliza Venderry Grace Charity Mary Cartir Greisnaningsi Greisnaningsi Harefa, Dellania Yvonne HARI HARTIKO Helen Melenia Sianipar Herna Siahaan Hernawati Siahaan Hutauruk, Kristian I Made Samudra Imanuel Adhitya Wulanata, Imanuel Adhitya Intan Cidarbulan Matita Iren Asima Situmorang Jennie Agatherania Listira JOEDORO SOEDARSONO Josephine Elsa Diani Putranto Karnelasatri, . Keisha Hillary Pangalila Kosi, Ice Frans Krisdarwindah Mardiana Kristin Armis Pasaribu Laia , Clement Khrisman Laia, Clement Khrisman Laoli, Beta Mualiman Lastiar R. Sitompul Lastiar Roselyna Sitompul, Lastiar Roselyna Legesang, Eddowardho Otniel Lucia Kusumawati Lusiana Haris Maha, Paldevina Br Maharaja, Dwi P. Malau, Robeka Risky Manalu, Damai Y. Manalu, Damai Yanti Marcellie, Jessica Margaretha, Cecilia Marvella, Eunike Bunga Melany Djami Meyners, Geraldy John Mia Parhusip Muhamad Ananda Munthe, Ashiong P. Munthe, Sri Wahyu Ningsih Naomi Dwany Nexson Parhusip, Adolf Jan Nicolas Tunggul Adhigandewa Nida Sopiah Nida Sopiah Nida Sopiah Nommensen Pangihutan Ompusunggu Nommensen Pangihutan Ompusunggu Novera, Rusliani S. Novera, Rusliani Sesy Orah, Eklesia Taysa Pahotkon Purba Pangaribuan, Medeliene Christine Patricia Patricia Pebrianti Pebrianti, Pebrianti Pingkan Imelda Wuisan Polin Parulian Ambarita Ponagadi, Merry Adisty Prasetyo, Djoko Purba, Friska Juliana Purwanti, Ester Ayu Sri PutrI, Nadya Aurelia Ratna Rahmawati, Sintia Y. Rahul Simon Situmeang Rani Nurlina Tifen Raudhatunnur Reinhard Pinontoan Resky Megawati Tammu Resti Nopriyanti Rido, Elisabeth M.T. Rimpan, Takashi Reymond Rosiva Betaria Purba Ruben Patrick Adhiwijaya Ruth Y. Purba Sabar Manampin Giawa Salomo Christian Selvi Esther Suwu, Selvi Esther Shaday, Vannesa El Sherina Juliana Sherina Juliana Siane Indriani, Siane Siane Indriyani Sianturi, Golda Santiur Silaban, Grace Niche Agnesa Silalahi, Ramses Silvana Putri Tarigan Simamora, Tanti Marsanda Sindy Friska Siringo-ringo, Sisilya Situmorang, Stefani Situmorang, Widya Srigika Natalia Boru Ginting Stephanus Jordan Jans Steven Anggawinata Sudarsono Siburian Sugata, Marcelia Suhartono, Andreas Valiant Sukri, Urbanus Susi Sulistia Susi Sulistia, Susi Tabitha Grace Larasati Tahya, Candra Tahya, Candra J. Tahya, Candra Yulius Tammu, Reisky Megawati Thesalonika Liony Pangemanan Tinambunan , Christine Febriandini Torreno, Jovan Nathanael Tri P. Priyatno Triwibowo Yuwono Valiant, Andreas Vania Austine Callista Timotius Vannesa El Shaday Ruth Advenita Viona Heryani Arla Waruwu, A'azokhi Waruwu, Aazokhi Wuisan, Pingkan Imelda Xaveria, Jessica Yadi Suryadi Yantra Wijaya YENNY SORAYA Yesica C. Rhenata Yohana Ayustin Yohanes Edi Gunanto Yolanda, Shelomita Feby Yovanka P.P Tumakaka Zebua, Satria Putri Aryani Zega, Yeni Angelina