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All Journal Jurnal Ilmiah Farmasi Journal of Biomedicine and Translational Research Biota Jurnal Sains dan Teknologi Pangan Science and Technology Indonesia Jurnal Biologi Tropis Martabe : Jurnal Pengabdian Kepada Masyarakat Hearty : Jurnal Kesehatan Masyarakat Jurnal Kreativitas PKM Jurnal Ilmu Kefarmasian Indonesia Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Jurnal Kesmas dan Gizi (JKG) Majalah Farmasetika CERATA Jurnal Ilmu Farmasi Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ners Jurnal Riset Kefarmasian Indonesia HSG (Health Science Growth) Journal Janaka : Jurnal Pengabdian Masyarakat Lumbung Farmasi : Jurnal Ilmu Kefarmasian Indobiosains EEICT (Electric, Electronic, Instrumentation, Control, Telecommunication) Jurnal Ilmiah Wahana Pendidikan Jurnal Pijar MIPA Jurnal Farmasetis Journal of Pharmaceutical and Sciences. Parapemikir Jurnal Ilmiah Farmasi Media Farmasi CERATA Jurnal Sains dan Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia PharmaCine: Journal of Pharmacy, Medical and Health Science Jurnal Abdimas Mahakam
Ahsanal Kasasiah
Universitas Singaperbangsa Karawang
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Dentistry Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Genetic Studies On The GHSR and IGF1R and Their Relationship With Stunting: A Systematic Literature Review Indratno, Saarah Hamidah Asmara; Malau, Jekmal; Kasasiah, Ahsanal
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i2.5731

Abstract

Stunting, also known as short stature, is a condition that occurs in children where children grow too short for their age. It can be influenced by several external and internal factors. This study explored various databases including Pubmed, Science Direct, Sage Journal, Springer Link, with keywords: "stunting OR short stature AND GHSR AND IGF1R AND genetic". There are 114 articles that match the PICOTS elements to be further reviewed using the PRISMA diagram. In this review article, 10 research articles published in 2011-2024 were used as sources of information. This review article discusses that internal factors of stunting can be caused by genetic factors influenced by growth genes, one of which is GHSR and IGF1R. Whole genome sequencing revealed potential genes involved in the growth pathway that showed an association between genetic variation in GHSR and IGF with the risk of the body's production or response to growth hormone that causes delayed child growth. The evidence highlights the possibility that these putative genetic markers could offer more effective treatment by focusing on the pathophysiology associated with development stunting. Due to lack of genetic studies on stunting genes carried out in Asian nations, genetic testing has not been used in clinical practice as a routine evaluation in the current national implementation, especially in Indonesia
Comparison of Two PCR Primer Sets for In-House Validation of GHSR Gene Variation Detection Employing Artificial Recombinant Plasmid Approach Ahsanal Kasasiah; Jekmal Malau; Sekar Andjung Tresnawati; Priscinya Christiana Debora; Nur Komala Fitri; Saarah Hamidah Asmara Indratno; Asman Hitopik; Eriyanti Astika; Anisa Aula Rahma; Fikri, Al Mukhlas
Biota Vol 10 No 2 (2024): Jurnal Biota 2024
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v10i2.21166

Abstract

Stunting is a significant global public health problem caused by long-term dietary deficits that affect many children worldwide. Both environmental and genetic factors, including variants in the GHSR gene, play a crucial role in stunted growth. This study used an artificial recombinant plasmid DNA method to evaluate two primer set combinations for identifying DNA variants in the GHSR gene. Selecting suitable primer sets for identifying GHSR genetic variants linked to stunting is essential, as evidenced by PCR and sequencing techniques. The target gene, based on the GHSR reference sequence, consists of eight DNA variations (ΔQ36, G57G, P108L, L118L, R159R, C173R, D246A, and A277P). A recombinant plasmid was created by inserting a 1000 bp fragment of the GHSR gene into the pUC57 backbone. Primer sets were chosen based on their capacity to amplify these eight genetic variations and were optimized and validated using PCR methods. PCR and bi-directional sequencing verified the existence of surrounding DNA and specific single nucleotide variants (SNVs). In our study, we discovered four changes in the DNA sequence (R159R G>A, C173R T>C, D246A A>C, and A277P G>C) using the E1_F2/E1_R3 primer pair. Additionally, a new combination of primers (E1_F1/E1_R3) effectively detected seven DNA sequence mutations (ΔQ36 del CAG, G57G C>T, P108L C>T, L118L C>T, R159R G>A, C173R T>C, and D246A A>C). We have developed a new combination of forward and reverse primers to identify seven SNVs in the GHSR gene, which could serve as a diagnostic tool in clinical laboratory settings.
Gut Microbiota Types between Well-Grown and Stunted Children: A Systematic Review Astika, Eriyanti; Kasasiah, Ahsanal; Malau, Jekmal; Fikri, Al Mukhlas
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i2.5871

Abstract

Stunting, one of the malnutrition in the prenatal period up to the first two years of life and often exacerbated by recurrent gastrointestinal infections after birth. This study explores from various databases including Pubmed, Scopus, Science Direct, Sage Journal, Springer Link, with keywords: "stunting, gut microbiota, toddler". In this study, 10 open access articles published in 2016-2024 were used as sources of information using PRISMA 2020 guideline. It was found that the presence of gut microbiota plays a role in stunting. Gastrointestinal infections have specific mechanisms that can lead to impaired growth and postnatal growth. Deficiency of non-pathogenic gut microbiota is considered to be the main cause. Changes in gut bacterial composition in children under five are associated with increased gastrointestinal frequency, which in turn leads to chronic growth disorders.
Influence of LEAP2 (Liver Expressed Antimicrobial Peptide-2) and Ghrelin Binding to GHSR Gene Receptor as Factors Obesity Incidence: A Literature Review Hitopik, Asman; Kasasiah, Ahsanal; Malau, Jekmal
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i3.5834

Abstract

Obesity is a serious issue in global health, which requires a more comprehensive understanding for the development of effective therapies. This study explores the role of Liver-Expressed Antimicrobial Peptide 2 (LEAP2) and its interaction with Ghrelin in regulating Growth Hormone Secretagogue Receptor (GHSR) as a potential obesity event. This method used in this review is a systematic literature analysis. The results obtained show that LEAP2, originally known as an antimicrobial, plays a significant role in metabolic regulation and body weight regulation. LEAP2 acts as a Ghrelin antagonist, reduces orexigenic effects and inhibits food intake, and exerts positive effects in tackling the effects of obesity such as hyperlipidemia and inflammation. The study also highlighted the potential of LEAP2 as a therapeutic target in obesity treatment, with palmitoylation modification showing increased stability and effectiveness of LEAP2. However, further understanding and clinical trial studies are needed to validate the preclinical findings and evaluate the long-term effects of LEAP2 regulation on human body weight and metabolic health, thus providing a basis for exploring potential clinical applications in future anti-obesity drug development
Marine-Derived Chitosan Biopolymers as Antibacterial Agents: A Review Nurhadis, Nurhadis; Kasasiah, Ahsanal; Hitopik, Asman
Jurnal Pijar Mipa Vol. 19 No. 6 (2024): November 2024
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v19i6.8021

Abstract

The growing issue of bacterial resistance to conventional antibiotics has led to an increasing need for alternative antimicrobial agents. Chitosan, a biopolymer derived from marine organisms such as crustaceans (shrimp, crabs) and mollusks (shellfish), has shown significant antibacterial properties. This systematic review aims to evaluate the antibacterial activity of chitosan extracted from various marine sources against Gram-positive (Staphylococcus epidermidis) and Gram-negative (Escherichia coli) bacteria. A comprehensive search of studies published in the past decade was conducted across multiple databases, using predefined inclusion criteria to identify relevant experimental research that focused on quantitative data, such as inhibition zones. The review analyzes key variables, including chitosan extraction methods, concentrations, and experimental conditions. The results revealed that chitosan exhibited the highest antibacterial activity against E. coli, while S. epidermidis showed moderate susceptibility. Variations in antibacterial effectiveness were attributed to differences in chitosan extraction methods and experimental conditions. Despite these variations, the overall evidence supports the potential of chitosan as an effective antimicrobial agent, demonstrating significant inhibition against a wide range of bacterial strains. The findings suggest that chitosan may serve as a promising natural alternative to combat bacterial infections, particularly those caused by antibiotic-resistant pathogens. However, further research is needed to standardize extraction techniques, explore the antibacterial mechanisms, and conduct in vivo studies to validate its clinical applications. These findings underscore chitosan's potential as a sustainable and effective solution in addressing the global challenge of bacterial resistance.
Tinjauan Literatur Sistematis; Pemanfaatan Teknologi CRISPR-CAS9 Untuk Pengobatan Penyakit Huntington’s Sari, Meliana; Kasasiah, Ahsanal
Media Farmasi Vol 20 No 2 (2024): Media Farmasi Edisi Oktober 2024
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v20i2.704

Abstract

Huntington's Disease (HD) is a neurodegenerative disorder caused by a CAG triple expansion (>36) in the first exon of the HTT gene encoding the huntingtin protein. Advances in gene editing technologies, such as CRISPR-Cas9, provide new hope for correcting the genetic mutations underlying HD. This review article aims to review the potential and effectiveness of CRISPR-Cas9 technology as a therapeutic tool for HD. The writing method used was Systematic Literature Review, with literature searches conducted using three databases: PubMed, ScienceDirect, and SpringerLink. This study reviewed several studies that used in vivo and in vitro models to evaluate the impact of HTT gene editing on mutant huntingtin protein expression and HD symptoms. Results showed that CRISPR-Cas9 can effectively reduce mutant huntingtin protein expression, reduce neuronal toxicity, and improve motor symptoms in mouse models of HD. Although these results are promising, further studies are needed to optimize the safety and effectiveness of using CRISPR-Cas9 in genetic therapy for HD.
Primer Design of Porcine DNA using Mitochondrial DNA (Cyt b Gene) for Halal Authentication using Polymerase Chain Reaction (PCR) Rohmah, Siti; Irwansyah, Silvana Lestari; Kasasiah, Ahsanal; Malau, Jekmal
Jurnal Sains dan Kesehatan Vol. 7 No. 1 (2025): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v7i1.2418

Abstract

The Muslim community has difficulties in determining the halal status of animal-derived items and their derivatives due to their extensive prevalence. Islamic customers are increasingly becoming more selective and are demanding halal certification for culinary goods. Developing a way to identify porcine DNA in goods is of utmost importance. The Cyt b gene is being used to evaluate swine DNA samples by using primer candidates. The process of generating primers utilizing bioinformatics tools, such as NCBI, MegaXI, Primer3Plus, SnapGene Viewer, and Net Primer websites, is utilized to assess and analyze the effectiveness of laboratory research. The study concluded that the primers effectively amplified pig DNA, but they were unsuccessful in amplifying chicken or beef DNA. After conducting in silico experiments, a total of 7 possible primers were generated. The most advantageous pair of primers was identified, which includes the forward primer 5'-AACATCCGAAATCACACCC-3' and the reverse primer 5'-AGAATGATATTTGTCCTCAGGG-3'. The efficacy of these primers was evaluated in a controlled laboratory environment. Results from the laboratory experiments demonstrate that these particular primers have the ability to amplify the Cyt b gene from the Sus scrofa species at a temperature of 58°C, producing a DNA fragment that is 415 base pairs long. DNA sequencing is essential to verify that the amplified DNA band matches to the Sus scrofa Cyt b gene. Keywords:          PCR, Porcine, Primer, Cyt b, Halal
Increasing Understanding of Medicines in the Community Through the Implementation of DAGUSIBU as a Prevention of Antibiotic Resistance in Pasir Jengkol Village, Karawang Regency Zahra, Aliya Azkia; Malau, Jekmal; Kasasiah, Ahsanal; Ratnasari, Devi; Septiani, Dia; Sholih, Mally Ghinan
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 8, No 5 (2025): Volume 8 No 5 (2025)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v8i5.18936

Abstract

ABSTRACT Antibiotic resistance occurs when microorganisms become unresponsive to antibiotic treatment, rendering common therapies ineffective against infections. As a result, the infection becomes increasingly challenging to treat, posing a heightened risk of severe disease progression and increasing the risk of spreading the disease. The causes of the emergence of antibiotic resistance include a number of factors, including excessive use of antibiotics from self-medication, to inappropriate disposal of antibiotic drugs. Karawang Regency, a region in West Java which consists of 10 sub-districts and has a population of more than 2.4 million people. Research conducted in Karawang shows the potential for antibiotic resistance based on use and the environment. It is known that the people of Karawang Regency have poor knowledge and perception regarding antibiotic resistance and use, one of which is Pasir Jengkol village. Therefore, it is necessary to increase understanding of medicines among the people of Karawang Regency, including by socializing the DAGUSIBU movement (Dapatkan, Gunakan, Simpan dan Buang Obat) or Proper Use, Storage and Disposal of Medications and its implementation as a measure to prevent antibiotic resistance. The output of this activity is primarily an increase in understanding of public knowledge through questionnaires which are analyzed using statistics. The results obtained were then tested using a statistical test showing a significant influence (Sig 0.002) increase in knowledge through the difference in pre-test and post-test scores. This outreach activity, which provides educational materials on preventing resistance, has positively impacted the Pasir Jengkol community's knowledge. Therefore, this socialization initiative can be replicated in other areas, particularly in Karawang Regency. Keywords: Antibiotic,  Drug Resistance, DAGUSIBU, Karawang.
Development of Species-Specific Primers Targeting Mitochondrial Cyt b Gene for Porcine DNA Detection in Halal Authentication via Polymerase Chain Reaction (PCR) Malau, Jekmal; Kasasiah, Ahsanal; Zahra, Aliya Azkia; Irwansyah, Silvana Lestari; Siboro, Dewi Pratiwi Purba
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.6799

Abstract

Background: The detection of porcine DNA is critical to ensuring adherence to halal standards, particularly in food and pharmaceutical products. This study aimed to design and validate species-specific primers targeting the mitochondrial Cyt b gene of Sus scrofa for porcine DNA identification. Using in silico tools such as NCBI, Primer3Plus, SnapGene, Mega and NetPrimer, four primer pairs were designed and assessed for specificity and efficiency. Methodology: Laboratory validation involved PCR amplification and bi-directional Sanger sequencing. Findings: The findings demonstrated that primers 2F/2R and 3F/3R successfully amplified the target DNA, producing amplicons of 514 bp and 456 bp, respectively. The primers exhibited high specificity, with no amplification observed in non-target DNA samples, including chicken and beef. Sanger sequencing confirmed that the amplified products corresponded to the Cyt b gene sequence of Sus scrofa with 100 % similarity, as validated through BLAST analysis. This study presents an accurate and dependable molecular method for detecting porcine DNA, with valuable applications in halal authentication and molecular diagnostics. Contribution: The developed primers offer an effective tool for accurately identifying porcine-derived components, addressing the critical demand for species-specific DNA detection to support halal compliance.
UTILIZATION OF CHIMERIC ANTIGEN RECEPTOR-T (CAR-T) CELL THERAPY AS AN INNOVATIVE THERAPY FOR NON-SMALL LUNG CANCER (NSLC) Rusdiawan, Fairuz Octora Aulia; Kasasiah, Ahsanal
HEARTY Vol 13 No 3 (2025): JUNI
Publisher : Fakultas Ilmu Kesehatan, Universitas Ibn Khaldun, Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/hearty.v13i3.19878

Abstract

Non-Small Lung Cancer is one of the types of lung cancer with the highest prevalence in Indonesia. So that treatment is needed in an effort to reduce the prevalence rate. Chimeric Antigen Receptor-T (CAR-T) cell therapy is known to have the potential to be an alternative treatment for these types of cancer cells. This research is made with the hope that in the future this research can be further developed by researchers, especially in Indonesia. This literature study was made using the Systematic Literature Review (SLR) design to determine the effectiveness of CAR-T cell therapy targeted at several different genes and tested on mouse xenograft models. The journals used were obtained with a publication range of 2014-2024 and 7 journals were selected based on the results of inclusion and exclusion. Based on the results of the article review, it is known that CAR-T cell therapy targeting genes has good antitumor activity efficacy obtained from the test results by measuring the volume and weight of tumors in mouse xenograft models using a caliper and Bioluminescence Imaging (BLI) irradiation method.
Co-Authors Adi Pancoro Adinda Christianti Suparno Adrian Hartanto, Adrian Aida, Fitri Ainaputri, Aliza Salsabila Aliya Azkia Zahra Anisa Aula Rahma Anisa Aula Rahma Annajla, Fathina Aprianti, Endeh Aprillia , Cantika Aprillia, Cantika Apriyanti , Endeh Asman Hitopik Astika, Eriyanti Cartas Cartas Dadang Sumardi Damara, Dandy Satria Daniel Joko Wahyono Devi - Ratnasari Devi Ratnasari Devi Ratnasari Dinda Nugrahan Diniarti, Fuji Ayu Eriyanti Astika Fika Setra Rikantara Fikri, Al Mukhlas Fuji Ayu Diniarti Hasna, Vina Luthfiana Hendiana, Siti Nurazizah Aghisna Hermosaningtyas, Anastasia Aliesa Hifdzil Qur’ani Hitopik, Asman Indah Laily Indah Laily Hilmi Indratno, Saarah Hamidah Asmara insani abadi bangsa Irgi, Khaeru Irwansyah, Silvana Lestari Jekmal Malau Jekmal Malau Jekmal Malau Lely Sulfiani Saula Lestari, Agatha Nabilla Malau , Jekmal Mally G Sholih Mally Ghinan Sholih Manalu, Rosario Trijuliamos Marsah Rahmawati Utami Meilani, Nanda Diva Meisya Diffa Amalia Putri Meliana Sari, Meliana Muhammad Naufal Nurhadi Hidayat Muhareva Raekiansyah Nabila Rubinadzari Nasywa Khoirun Nisa Nugraha , Afif Tri Nugraha, Afif Tri Nur Khafipah Nur Komala Fitri Nuraeni Putri Nurhadis, Nurhadis Nurzamani, Salsabila Pramudya Pratama Priscinya Christiana Debora Rahmasari, Ratika Ratnasari, Devi Reas Najla Anugrah Rivandia Listi Rizky Abdul Majid Rosa Arum Rusdiawan, Fairuz Octora Aulia Rusmayanti, Agustina Saarah Hamidah Asmara Indratno Salman Salman Saryono Sekar Andjung Tresnawati Septi, Annisa Frastica Septiani, Dia Sholih, Mally Ghinan Siboro , Dewi Pratiwi Purba Siboro, Dewi Pratiwi Purba Siti Nurazizah Aghisna Hendiana Siti Rohmah Sophy Wulandari Sudarjat, Hadi Sulastri Sulastri Syeli Syawaliyah Urbaningrum, Lestari Mahardika Vesara Ardhe Gatera Zahra, Aliya Azkia Zahro, Aurora Fatimatuz