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All Journal Jurnal Ilmiah Farmasi Journal of Biomedicine and Translational Research Biota Jurnal Sains dan Teknologi Pangan Science and Technology Indonesia Jurnal Biologi Tropis Martabe : Jurnal Pengabdian Kepada Masyarakat Hearty : Jurnal Kesehatan Masyarakat Jurnal Kreativitas PKM Jurnal Ilmu Kefarmasian Indonesia Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Jurnal Kesmas dan Gizi (JKG) Majalah Farmasetika CERATA Jurnal Ilmu Farmasi Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ners Jurnal Riset Kefarmasian Indonesia HSG (Health Science Growth) Journal Janaka : Jurnal Pengabdian Masyarakat Lumbung Farmasi : Jurnal Ilmu Kefarmasian Indobiosains EEICT (Electric, Electronic, Instrumentation, Control, Telecommunication) Jurnal Ilmiah Wahana Pendidikan Jurnal Pijar MIPA Jurnal Farmasetis Health Research Journal of Indonesia Journal of Pharmaceutical and Sciences. Parapemikir Jurnal Ilmiah Farmasi Media Farmasi CERATA Jurnal Sains dan Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia PharmaCine: Journal of Pharmacy, Medical and Health Science Jurnal Abdimas Mahakam
Ahsanal Kasasiah
Universitas Singaperbangsa Karawang
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Dentistry Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Comparison of Two PCR Primer Sets for In-House Validation of GHSR Gene Variation Detection Employing Artificial Recombinant Plasmid Approach Ahsanal Kasasiah; Jekmal Malau; Sekar Andjung Tresnawati; Priscinya Christiana Debora; Nur Komala Fitri; Saarah Hamidah Asmara Indratno; Asman Hitopik; Eriyanti Astika; Anisa Aula Rahma; Fikri, Al Mukhlas
Biota Vol 10 No 2 (2024): Jurnal Biota 2024
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v10i2.21166

Abstract

Stunting is a significant global public health problem caused by long-term dietary deficits that affect many children worldwide. Both environmental and genetic factors, including variants in the GHSR gene, play a crucial role in stunted growth. This study used an artificial recombinant plasmid DNA method to evaluate two primer set combinations for identifying DNA variants in the GHSR gene. Selecting suitable primer sets for identifying GHSR genetic variants linked to stunting is essential, as evidenced by PCR and sequencing techniques. The target gene, based on the GHSR reference sequence, consists of eight DNA variations (ΔQ36, G57G, P108L, L118L, R159R, C173R, D246A, and A277P). A recombinant plasmid was created by inserting a 1000 bp fragment of the GHSR gene into the pUC57 backbone. Primer sets were chosen based on their capacity to amplify these eight genetic variations and were optimized and validated using PCR methods. PCR and bi-directional sequencing verified the existence of surrounding DNA and specific single nucleotide variants (SNVs). In our study, we discovered four changes in the DNA sequence (R159R G>A, C173R T>C, D246A A>C, and A277P G>C) using the E1_F2/E1_R3 primer pair. Additionally, a new combination of primers (E1_F1/E1_R3) effectively detected seven DNA sequence mutations (ΔQ36 del CAG, G57G C>T, P108L C>T, L118L C>T, R159R G>A, C173R T>C, and D246A A>C). We have developed a new combination of forward and reverse primers to identify seven SNVs in the GHSR gene, which could serve as a diagnostic tool in clinical laboratory settings.
Marine-Derived Chitosan Biopolymers as Antibacterial Agents: A Review Nurhadis, Nurhadis; Kasasiah, Ahsanal; Hitopik, Asman
Jurnal Pijar Mipa Vol. 19 No. 6 (2024): November 2024
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v19i6.8021

Abstract

The growing issue of bacterial resistance to conventional antibiotics has led to an increasing need for alternative antimicrobial agents. Chitosan, a biopolymer derived from marine organisms such as crustaceans (shrimp, crabs) and mollusks (shellfish), has shown significant antibacterial properties. This systematic review aims to evaluate the antibacterial activity of chitosan extracted from various marine sources against Gram-positive (Staphylococcus epidermidis) and Gram-negative (Escherichia coli) bacteria. A comprehensive search of studies published in the past decade was conducted across multiple databases, using predefined inclusion criteria to identify relevant experimental research that focused on quantitative data, such as inhibition zones. The review analyzes key variables, including chitosan extraction methods, concentrations, and experimental conditions. The results revealed that chitosan exhibited the highest antibacterial activity against E. coli, while S. epidermidis showed moderate susceptibility. Variations in antibacterial effectiveness were attributed to differences in chitosan extraction methods and experimental conditions. Despite these variations, the overall evidence supports the potential of chitosan as an effective antimicrobial agent, demonstrating significant inhibition against a wide range of bacterial strains. The findings suggest that chitosan may serve as a promising natural alternative to combat bacterial infections, particularly those caused by antibiotic-resistant pathogens. However, further research is needed to standardize extraction techniques, explore the antibacterial mechanisms, and conduct in vivo studies to validate its clinical applications. These findings underscore chitosan's potential as a sustainable and effective solution in addressing the global challenge of bacterial resistance.
Tinjauan Literatur Sistematis; Pemanfaatan Teknologi CRISPR-CAS9 Untuk Pengobatan Penyakit Huntington’s Sari, Meliana; Kasasiah, Ahsanal
Media Farmasi Vol 20 No 2 (2024): Media Farmasi Edisi Oktober 2024
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v20i2.704

Abstract

Huntington's Disease (HD) is a neurodegenerative disorder caused by a CAG triple expansion (>36) in the first exon of the HTT gene encoding the huntingtin protein. Advances in gene editing technologies, such as CRISPR-Cas9, provide new hope for correcting the genetic mutations underlying HD. This review article aims to review the potential and effectiveness of CRISPR-Cas9 technology as a therapeutic tool for HD. The writing method used was Systematic Literature Review, with literature searches conducted using three databases: PubMed, ScienceDirect, and SpringerLink. This study reviewed several studies that used in vivo and in vitro models to evaluate the impact of HTT gene editing on mutant huntingtin protein expression and HD symptoms. Results showed that CRISPR-Cas9 can effectively reduce mutant huntingtin protein expression, reduce neuronal toxicity, and improve motor symptoms in mouse models of HD. Although these results are promising, further studies are needed to optimize the safety and effectiveness of using CRISPR-Cas9 in genetic therapy for HD.
Primer Design of Porcine DNA using Mitochondrial DNA (Cyt b Gene) for Halal Authentication using Polymerase Chain Reaction (PCR) Rohmah, Siti; Irwansyah, Silvana Lestari; Kasasiah, Ahsanal; Malau, Jekmal
Jurnal Sains dan Kesehatan Vol. 7 No. 1 (2025): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v7i1.2418

Abstract

The Muslim community has difficulties in determining the halal status of animal-derived items and their derivatives due to their extensive prevalence. Islamic customers are increasingly becoming more selective and are demanding halal certification for culinary goods. Developing a way to identify porcine DNA in goods is of utmost importance. The Cyt b gene is being used to evaluate swine DNA samples by using primer candidates. The process of generating primers utilizing bioinformatics tools, such as NCBI, MegaXI, Primer3Plus, SnapGene Viewer, and Net Primer websites, is utilized to assess and analyze the effectiveness of laboratory research. The study concluded that the primers effectively amplified pig DNA, but they were unsuccessful in amplifying chicken or beef DNA. After conducting in silico experiments, a total of 7 possible primers were generated. The most advantageous pair of primers was identified, which includes the forward primer 5'-AACATCCGAAATCACACCC-3' and the reverse primer 5'-AGAATGATATTTGTCCTCAGGG-3'. The efficacy of these primers was evaluated in a controlled laboratory environment. Results from the laboratory experiments demonstrate that these particular primers have the ability to amplify the Cyt b gene from the Sus scrofa species at a temperature of 58°C, producing a DNA fragment that is 415 base pairs long. DNA sequencing is essential to verify that the amplified DNA band matches to the Sus scrofa Cyt b gene. Keywords:          PCR, Porcine, Primer, Cyt b, Halal
Increasing Understanding of Medicines in the Community Through the Implementation of DAGUSIBU as a Prevention of Antibiotic Resistance in Pasir Jengkol Village, Karawang Regency Zahra, Aliya Azkia; Malau, Jekmal; Kasasiah, Ahsanal; Ratnasari, Devi; Septiani, Dia; Sholih, Mally Ghinan
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 8, No 5 (2025): Volume 8 No 5 (2025)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v8i5.18936

Abstract

ABSTRACT Antibiotic resistance occurs when microorganisms become unresponsive to antibiotic treatment, rendering common therapies ineffective against infections. As a result, the infection becomes increasingly challenging to treat, posing a heightened risk of severe disease progression and increasing the risk of spreading the disease. The causes of the emergence of antibiotic resistance include a number of factors, including excessive use of antibiotics from self-medication, to inappropriate disposal of antibiotic drugs. Karawang Regency, a region in West Java which consists of 10 sub-districts and has a population of more than 2.4 million people. Research conducted in Karawang shows the potential for antibiotic resistance based on use and the environment. It is known that the people of Karawang Regency have poor knowledge and perception regarding antibiotic resistance and use, one of which is Pasir Jengkol village. Therefore, it is necessary to increase understanding of medicines among the people of Karawang Regency, including by socializing the DAGUSIBU movement (Dapatkan, Gunakan, Simpan dan Buang Obat) or Proper Use, Storage and Disposal of Medications and its implementation as a measure to prevent antibiotic resistance. The output of this activity is primarily an increase in understanding of public knowledge through questionnaires which are analyzed using statistics. The results obtained were then tested using a statistical test showing a significant influence (Sig 0.002) increase in knowledge through the difference in pre-test and post-test scores. This outreach activity, which provides educational materials on preventing resistance, has positively impacted the Pasir Jengkol community's knowledge. Therefore, this socialization initiative can be replicated in other areas, particularly in Karawang Regency. Keywords: Antibiotic,  Drug Resistance, DAGUSIBU, Karawang.
UTILIZATION OF CHIMERIC ANTIGEN RECEPTOR-T (CAR-T) CELL THERAPY AS AN INNOVATIVE THERAPY FOR NON-SMALL LUNG CANCER (NSLC) Rusdiawan, Fairuz Octora Aulia; Kasasiah, Ahsanal
HEARTY Vol 13 No 3 (2025): JUNI
Publisher : Fakultas Ilmu Kesehatan, Universitas Ibn Khaldun, Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/hearty.v13i3.19878

Abstract

Non-Small Lung Cancer is one of the types of lung cancer with the highest prevalence in Indonesia. So that treatment is needed in an effort to reduce the prevalence rate. Chimeric Antigen Receptor-T (CAR-T) cell therapy is known to have the potential to be an alternative treatment for these types of cancer cells. This research is made with the hope that in the future this research can be further developed by researchers, especially in Indonesia. This literature study was made using the Systematic Literature Review (SLR) design to determine the effectiveness of CAR-T cell therapy targeted at several different genes and tested on mouse xenograft models. The journals used were obtained with a publication range of 2014-2024 and 7 journals were selected based on the results of inclusion and exclusion. Based on the results of the article review, it is known that CAR-T cell therapy targeting genes has good antitumor activity efficacy obtained from the test results by measuring the volume and weight of tumors in mouse xenograft models using a caliper and Bioluminescence Imaging (BLI) irradiation method.
Advancements in Real-Time PCR Technologies: A Comprehensive Review of Probe-Based and Non-Probe-Based Assays for Molecular Diagnostics Malau, Jekmal; Zahro, Aurora Fatimatuz; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Meilani, Nanda Diva; Damara, Dandy Satria; Lestari, Agatha Nabilla; Saryono; Wahyono, Daniel Joko
Science and Technology Indonesia Vol. 10 No. 3 (2025): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.3.660-677

Abstract

The decision between probe-based and non-probe-based qPCR assays is crucial, influenced by diagnostic goals and sample characteristics. This review provides an in-depth evaluation of these two assay types, analyzing their principles, strengths, drawbacks, and applications. A thorough review of the literature, primarily sourced from PubMed, was undertaken to explore prominent assay systems, including TaqMan, KASP, rhAmp, HRM, and SYBR Green. Probe-based qPCR assays, exemplified by TaqMan and rhAmp, are distinguished by their high specificity, aptitude for multiplex analysis, and reduced risk of false positives, making them highly suitable for SNP genotyping and pathogen detection. However, their elevated costs and intricate design requirements remain significant challenges. Conversely, non-probe-based assays, such as SYBR Green and HRM, present cost-effective alternatives with straightforward designs. HRM, in particular, is effective in identifying genetic variations like SNPs with remarkable sensitivity. Nonetheless, these methods are susceptible to non-specific amplifications, requiring careful optimization to maintain reliability. The selection of a suitable qPCR assay depends on various factors, including precision, affordability, and multiplexing capabilities, with applications spanning infectious disease detection and genetic disorder analysis. This review emphasizes the indispensable role of qPCR in molecular diagnostics while showcasing recent technological advances that aim to mitigate existing constraints and enhance diagnostic precision and accessibility.
Pemanfaatan Beberapa Tanaman sebagai Sumber Antioksidan dan Obat Alternatif untuk Diabetes Melitus Utami, Marsah Rahmawati; Sholih, Mally Ghinan; Kasasiah, Ahsanal
Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Vol 6, No 3 (2023): Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstormin
Publisher : Politeknik Harapan Bersama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30591/japhb.v6i3.4848

Abstract

World Health Organization (WHO) menyatakan bahwa diabetes melitus (DM) termasuk salah satu pembunuh terbesar di Asia tenggara dan Pasifik barat. Salah satu penyebab timbulnya penyakit diabetes melitus adalah Stres oksidatif dan kerusakan oksidatif pada jaringan. Indonesia merupakan negara megabiodiversity yang memiliki jenis tumbuhan yang berkhasiat sebagai obat. diantaranya adalah berkhasiat sebagai sumber antioksidan dan antidiabetes. Namun sayang kekayaan alam tersebut tampaknya masih belum dimanfaatkan secara optimal untuk kesehatan khususnya untuk pengobatan maupun pencegahan penyakit diabetes Mellitus. Desa Sindangmulya merupakan salah satu desa di kabupaten karawang yang tingkat penderita penyakit degenaratifnya cukup tinggi, yang diantaranya adalah diabetes mellitus. Berdasarkan hal itu, kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan masyarakat tentang  pemanfaatan tanaman herbal  sebagai sumber antioksidan dan antidiabetes. Kegiatan dilakukan dengan metode penyuluhan dan pelatihan. Hasil kegiatan pengabdian masyarakat yang telah dilakukan menunjukkan bahwa setelah dilakukan penyuluhan dan pelatihan, pengetahuan para peserta  tentang tanaman herbal dan pemanfaatannya sebagai sumber antioksidan dan antidiabetes serta cara pembuatannya meningkat dengan rata-rata peningkatan sebesar 127,4%.
The Relationship Between COMT and MAO-B Gene Polymorphisms with Levodopa in Parkinson’s Disease Patients; A Review Hasna, Vina Luthfiana; Kasasiah, Ahsanal; Manalu, Rosario Trijuliamos; Malau, Jekmal
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5228

Abstract

Parkinson's disease is a degenerative nervous system disorder caused by the death of dopamine-producing cells in the substantia nigra. Dopaminergic treatment could alleviate motor symptoms for a period. One of the effective dopaminergic medications for symptomatic relief was Levodopa and dopamine agonists. Clinically, Levodopa was always combined with Dopa Decarboxylase (DDC) inhibitors, which redirected Levodopa metabolism towards the COMT pathway, increasing its bioavailability in the central nervous system. The purpose of this article was to investigate the relationship between COMT and MAO-B gene polymorphisms and Levodopa in Parkinson's disease patients, starting by gathering literature on the association between COMT and MAO-B polymorphisms and Levodopa in Parkinson's disease patients using various databases. Reviewed literature revealed that the most frequent polymorphism in the COMT gene was rs4680. Some polymorphisms significantly impacted the treatment of Parkinson's disease patients. However, despite efforts to identify genetic factors influencing the risk of side effects or treatment ineffectiveness, the role of pharmacogenetics in Parkinson's disease has not been fully explored and lacks consistent clinical recommendations. Further research was needed to tailor treatment to individual polymorphisms so that pharmacogenomic approaches could be applied more consistently
DEVELOPMENT OF PLASMID-BASED FOR EXTERNAL CONTROL MATERIALS OF CYP2D6*10 (rs1065852) GENE PCR-BASED DETECTION Malau, Jekmal; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Rahmasari, Ratika; Raekiansyah, Muhareva; Rohmah, Siti; Meilani, Nanda Diva; Septi, Annisa Frastica; Zahro, Aurora Fatimatuz; Annajla, Fathina; Hermosaningtyas, Anastasia Aliesa; Hilmi, Indah Laily
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2557

Abstract

Reliable clinical diagnosis of Single Nucleotide Polymorphisms (SNPs) is necessary for personalizing tamoxifen medication according to CYP2D6*10 genetic variations. Our research aimed to create a recombinant plasmid for external control material with a molecular size of 3812 bp. The recombinant plasmid was achieved by cloning an 838 bp gene insert of CYP2D6*10 rs1065852 into a 2974 bp pJET1.2 plasmid into Escherichia coli DH10B and selection on ampicillin agar medium. Isolated E. coli recombinants provided the plasmid molecules for analysis. Bi-directional sequencing and Real-Time PCR confirmed the presence of wild-type and mutant rs1065852 DNA fragments in the plasmid, namely homozygote CC and TT. The conclusion is that we have successfully introduced a novel recombinant plasmid developed by cloning the SNP rs1065852, which carries the 100C>T mutation, using pJET 1.2/blunt system, which could significantly enhance the accuracy of clinical SNP diagnostics for personalized medicine in breast cancer treatment.
Co-Authors Adi Pancoro Adinda Christianti Suparno Adrian Hartanto, Adrian Afif Tri Nugraha Aida, Fitri Al Mukhlas Fikri Aliya Azkia Zahra Aliza Salsabila Ainaputri Anisa Aula Rahma Anisa Aula Rahma Annajla, Fathina Asman Hitopik Asman Hitopik Cantika Aprillia Cartas Cartas Dadang Sumardi Damara, Dandy Satria Daniel Joko Wahyono Devi - Ratnasari Devi Ratnasari Devi Ratnasari Dewi Pratiwi Purba Siboro Dinda Nugrahan Diniarti, Fuji Ayu Endeh Apriyanti Eriyanti Astika Eriyanti Astika Farhan Aimarsa Latuconsina Fika Setra Rikantara Fikri, Al Mukhlas Fuji Ayu Diniarti Hasna, Vina Luthfiana Hendiana, Siti Nurazizah Aghisna Hermosaningtyas, Anastasia Aliesa Hifdzil Qur’ani Hitopik, Asman Indah Laily Indah Laily Hilmi insani abadi bangsa Irgi, Khaeru Irwansyah, Silvana Lestari Jekmal Malau Jekmal Malau Jekmal Malau Lely Sulfiani Saula Lestari, Agatha Nabilla Mally G Sholih Mally Ghinan Sholih Manalu, Rosario Trijuliamos Marsah Rahmawati Utami Meilani, Nanda Diva Meisya Diffa Amalia Putri Meliana Sari, Meliana Muhammad Naufal Nurhadi Hidayat Muhareva Raekiansyah Nabila Rubinadzari Naila Fathna Nurul Hamidah Nasywa Khoirun Nisa Nur Khafipah Nur Komala Fitri Nuraeni Putri Nurhadis, Nurhadis Nurzamani, Salsabila Pramudya Pratama Priscinya Christiana Debora Rahmasari, Ratika Ratnasari, Devi Reas Najla Anugrah Rivandia Listi Rizky Abdul Majid Rosa Arum Rusdiawan, Fairuz Octora Aulia Rusmayanti, Agustina Saarah Hamidah Asmara Indratno Saarah Hamidah Asmara Indratno Salman Salman Saryono Sekar Andjung Tresnawati Septi, Annisa Frastica Septiani, Dia Sholih, Mally Ghinan Siti Nurazizah Aghisna Hendiana Siti Rohmah Sophy Wulandari Sudarjat, Hadi Sulastri Sulastri Syeli Syawaliyah Urbaningrum, Lestari Mahardika Vesara Ardhe Gatera Vriezka Mierza Zahra, Aliya Azkia Zahro, Aurora Fatimatuz