Garuda - Garba Rujukan Digital

Advancements in Real-Time PCR Technologies: A Comprehensive Review of Probe-Based and Non-Probe-Based Assays for Molecular Diagnostics Malau, Jekmal; Zahro, Aurora Fatimatuz; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Meilani, Nanda Diva; Damara, Dandy Satria; Lestari, Agatha Nabilla; Saryono; Wahyono, Daniel Joko
Science and Technology Indonesia Vol. 10 No. 3 (2025): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.3.660-677

The decision between probe-based and non-probe-based qPCR assays is crucial, influenced by diagnostic goals and sample characteristics. This review provides an in-depth evaluation of these two assay types, analyzing their principles, strengths, drawbacks, and applications. A thorough review of the literature, primarily sourced from PubMed, was undertaken to explore prominent assay systems, including TaqMan, KASP, rhAmp, HRM, and SYBR Green. Probe-based qPCR assays, exemplified by TaqMan and rhAmp, are distinguished by their high specificity, aptitude for multiplex analysis, and reduced risk of false positives, making them highly suitable for SNP genotyping and pathogen detection. However, their elevated costs and intricate design requirements remain significant challenges. Conversely, non-probe-based assays, such as SYBR Green and HRM, present cost-effective alternatives with straightforward designs. HRM, in particular, is effective in identifying genetic variations like SNPs with remarkable sensitivity. Nonetheless, these methods are susceptible to non-specific amplifications, requiring careful optimization to maintain reliability. The selection of a suitable qPCR assay depends on various factors, including precision, affordability, and multiplexing capabilities, with applications spanning infectious disease detection and genetic disorder analysis. This review emphasizes the indispensable role of qPCR in molecular diagnostics while showcasing recent technological advances that aim to mitigate existing constraints and enhance diagnostic precision and accessibility.

Pemanfaatan Beberapa Tanaman sebagai Sumber Antioksidan dan Obat Alternatif untuk Diabetes Melitus Utami, Marsah Rahmawati; Sholih, Mally Ghinan; Kasasiah, Ahsanal
Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Vol 6, No 3 (2023): Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstormin
Publisher : Politeknik Harapan Bersama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30591/japhb.v6i3.4848

World Health Organization (WHO) menyatakan bahwa diabetes melitus (DM) termasuk salah satu pembunuh terbesar di Asia tenggara dan Pasifik barat. Salah satu penyebab timbulnya penyakit diabetes melitus adalah Stres oksidatif dan kerusakan oksidatif pada jaringan. Indonesia merupakan negara megabiodiversity yang memiliki jenis tumbuhan yang berkhasiat sebagai obat. diantaranya adalah berkhasiat sebagai sumber antioksidan dan antidiabetes. Namun sayang kekayaan alam tersebut tampaknya masih belum dimanfaatkan secara optimal untuk kesehatan khususnya untuk pengobatan maupun pencegahan penyakit diabetes Mellitus. Desa Sindangmulya merupakan salah satu desa di kabupaten karawang yang tingkat penderita penyakit degenaratifnya cukup tinggi, yang diantaranya adalah diabetes mellitus. Berdasarkan hal itu, kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan masyarakat tentang pemanfaatan tanaman herbal sebagai sumber antioksidan dan antidiabetes. Kegiatan dilakukan dengan metode penyuluhan dan pelatihan. Hasil kegiatan pengabdian masyarakat yang telah dilakukan menunjukkan bahwa setelah dilakukan penyuluhan dan pelatihan, pengetahuan para peserta tentang tanaman herbal dan pemanfaatannya sebagai sumber antioksidan dan antidiabetes serta cara pembuatannya meningkat dengan rata-rata peningkatan sebesar 127,4%.

The Relationship Between COMT and MAO-B Gene Polymorphisms with Levodopa in Parkinson’s Disease Patients; A Review Hasna, Vina Luthfiana; Kasasiah, Ahsanal; Manalu, Rosario Trijuliamos; Malau, Jekmal
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5228

Parkinson's disease is a degenerative nervous system disorder caused by the death of dopamine-producing cells in the substantia nigra. Dopaminergic treatment could alleviate motor symptoms for a period. One of the effective dopaminergic medications for symptomatic relief was Levodopa and dopamine agonists. Clinically, Levodopa was always combined with Dopa Decarboxylase (DDC) inhibitors, which redirected Levodopa metabolism towards the COMT pathway, increasing its bioavailability in the central nervous system. The purpose of this article was to investigate the relationship between COMT and MAO-B gene polymorphisms and Levodopa in Parkinson's disease patients, starting by gathering literature on the association between COMT and MAO-B polymorphisms and Levodopa in Parkinson's disease patients using various databases. Reviewed literature revealed that the most frequent polymorphism in the COMT gene was rs4680. Some polymorphisms significantly impacted the treatment of Parkinson's disease patients. However, despite efforts to identify genetic factors influencing the risk of side effects or treatment ineffectiveness, the role of pharmacogenetics in Parkinson's disease has not been fully explored and lacks consistent clinical recommendations. Further research was needed to tailor treatment to individual polymorphisms so that pharmacogenomic approaches could be applied more consistently

DEVELOPMENT OF PLASMID-BASED FOR EXTERNAL CONTROL MATERIALS OF CYP2D6*10 (rs1065852) GENE PCR-BASED DETECTION Malau, Jekmal; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Rahmasari, Ratika; Raekiansyah, Muhareva; Rohmah, Siti; Meilani, Nanda Diva; Septi, Annisa Frastica; Zahro, Aurora Fatimatuz; Annajla, Fathina; Hermosaningtyas, Anastasia Aliesa; Hilmi, Indah Laily
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2557

Reliable clinical diagnosis of Single Nucleotide Polymorphisms (SNPs) is necessary for personalizing tamoxifen medication according to CYP2D6*10 genetic variations. Our research aimed to create a recombinant plasmid for external control material with a molecular size of 3812 bp. The recombinant plasmid was achieved by cloning an 838 bp gene insert of CYP2D6*10 rs1065852 into a 2974 bp pJET1.2 plasmid into Escherichia coli DH10B and selection on ampicillin agar medium. Isolated E. coli recombinants provided the plasmid molecules for analysis. Bi-directional sequencing and Real-Time PCR confirmed the presence of wild-type and mutant rs1065852 DNA fragments in the plasmid, namely homozygote CC and TT. The conclusion is that we have successfully introduced a novel recombinant plasmid developed by cloning the SNP rs1065852, which carries the 100C>T mutation, using pJET 1.2/blunt system, which could significantly enhance the accuracy of clinical SNP diagnostics for personalized medicine in breast cancer treatment.

DEVELOPMENT OF PLASMID-BASED FOR EXTERNAL CONTROL MATERIALS OF CYP2D6*10 (rs1065852) GENE PCR-BASED DETECTION Malau, Jekmal; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Rahmasari, Ratika; Raekiansyah, Muhareva; Rohmah, Siti; Meilani, Nanda Diva; Septi, Annisa Frastica; Zahro, Aurora Fatimatuz; Annajla, Fathina; Hermosaningtyas, Anastasia Aliesa; Hilmi, Indah Laily
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2557

Reliable clinical diagnosis of Single Nucleotide Polymorphisms (SNPs) is necessary for personalizing tamoxifen medication according to CYP2D6*10 genetic variations. Our research aimed to create a recombinant plasmid for external control material with a molecular size of 3812 bp. The recombinant plasmid was achieved by cloning an 838 bp gene insert of CYP2D6*10 rs1065852 into a 2974 bp pJET1.2 plasmid into Escherichia coli DH10B and selection on ampicillin agar medium. Isolated E. coli recombinants provided the plasmid molecules for analysis. Bi-directional sequencing and Real-Time PCR confirmed the presence of wild-type and mutant rs1065852 DNA fragments in the plasmid, namely homozygote CC and TT. The conclusion is that we have successfully introduced a novel recombinant plasmid developed by cloning the SNP rs1065852, which carries the 100C>T mutation, using pJET 1.2/blunt system, which could significantly enhance the accuracy of clinical SNP diagnostics for personalized medicine in breast cancer treatment.

Green synthesis of silver nanoparticles using Pluchea (Pluchea indica (L.)) leaf extract and antibacterial activity against Staphylococcus aureus and Propionibacterium acnes Ratnasari, Devi; Kasasiah, Ahsanal; Sulastri, Sulastri; Aida, Fitri
JURNAL ILMU KEFARMASIAN INDONESIA Vol 23 No 2 (2025): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v23i2.1648

Nanotechnology is widely used in the biomedical purpose as a drug delivery system, cancer, and tumor biomarkers. Currently, metals are most used as precursor agents to form nanoparticles such as silver, gold, iron, zinc, and metal oxides. Acne is one of the skin problems caused by the growth of S. aureus and P. acnes bacteria. Treatment of acne using inappropriate antibiotics can lead to resistance. Silver nanoparticles are known to have the ability to kill pathogenic microorganisms. Pluchea leaf extract contains flavonoid, polyphenol, and tannin compounds that can work as natural bioreductors in the formation of silver nanoparticles while inhibiting bacterial growth. This study aims to synthesize silver nanoparticles using Pluchea leaf extract and testing antibacterial activity against Staphylococcus aureus and Propionibacterium acnes. Extraction of Pluchea leaves was carried out using the infusion method with the water solvent. The synthesis of silver nanoparticles was used a shaker incubator at a speed of 150 rpm and a temperature of 37 ºC for 48 hours. Characterization of silver nanoparticles using uv-vis spectrophotometry, particle size analyzer (PSA), zeta potential analyzer, and FESEM-EDX. Antibacterial activity test using microdilution method. The characterization results of silver nanoparticles showed a particle size of 20.50 nm, a zeta potential of -38.6 mV, and a spherical morphological shape. Silver nanoparticles have antibacterial activity against S. aureus and P. acnes with a Minimum Inhibitory Concentration (MIC) value of 62.5 ppm and Minimum Bactericidal Concentration (MBC) value >250 ppm.

Probiotic Gummies for Improving Gut Microbiota and Nutritional Status in Stunted Children: A Narrative Review Rusmayanti, Agustina; Kasasiah, Ahsanal; Irgi, Khaeru
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10708

Stunting remains a major nutritional problem in Indonesia and is often linked to gut microbiota imbalance that can limit nutrient absorption and weaken intestinal function. This article reviews evidence on how probiotics can support gut health and examines the potential of gummy formulations that children can consume easily. The review process used Publish or Perish to search scientific articles from 2016 to 2025 in Google Scholar, PubMed and Scopus. Titles, abstracts and full texts were screened to ensure relevance. A total of 17 studies were included in the analysis. The findings show that probiotics can increase SCFA-producing bacteria, improve the gut mucosal barrier and support the absorption of minerals such as calcium and iron. Several studies also report better digestive comfort and improved microbiota diversity after probiotic intake. Probiotic gummies show good stability, maintain viable cells and are well accepted by children, which makes them a practical delivery system. The evidence from these 17 studies suggests that probiotic gummies have potential to support gut health and contribute to stunting prevention. Longer studies are still needed to measure their direct impact on child growth and guide future nutritional interventions.

Green synthesis of silver nanoparticles using Pluchea (Pluchea indica (L.)) leaf extract and antibacterial activity against Staphylococcus aureus and Propionibacterium acnes Ratnasari, Devi; kasasiah, Ahsanal; Sulastri, Sulastri; Aida, Fitri
JURNAL ILMU KEFARMASIAN INDONESIA Vol. 23 No. 2 (2025): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v23i2.1648

Nanotechnology is widely used in the biomedical purpose as a drug delivery system, cancer, and tumor biomarkers. Currently, metals are most used as precursor agents to form nanoparticles such as silver, gold, iron, zinc, and metal oxides. Acne is one of the skin problems caused by the growth of S. aureus and P. acnes bacteria. Treatment of acne using inappropriate antibiotics can lead to resistance. Silver nanoparticles are known to have the ability to kill pathogenic microorganisms. Pluchea leaf extract contains flavonoid, polyphenol, and tannin compounds that can work as natural bioreductors in the formation of silver nanoparticles while inhibiting bacterial growth. This study aims to synthesize silver nanoparticles using Pluchea leaf extract and testing antibacterial activity against Staphylococcus aureus and Propionibacterium acnes. Extraction of Pluchea leaves was carried out using the infusion method with the water solvent. The synthesis of silver nanoparticles was used a shaker incubator at a speed of 150 rpm and a temperature of 37 ºC for 48 hours. Characterization of silver nanoparticles using uv-vis spectrophotometry, particle size analyzer (PSA), zeta potential analyzer, and FESEM-EDX. Antibacterial activity test using microdilution method. The characterization results of silver nanoparticles showed a particle size of 20.50 nm, a zeta potential of -38.6 mV, and a spherical morphological shape. Silver nanoparticles have antibacterial activity against S. aureus and P. acnes with a Minimum Inhibitory Concentration (MIC) value of 62.5 ppm and Minimum Bactericidal Concentration (MBC) value >250 ppm.

Factors Associated with FMT Failure in Clostridioides difficile Infection and Age-Related Considerations: A Review Irgi, Khaeru; Kasasiah, Ahsanal; Rusmayanti, Agustina
Jurnal Pijar Mipa Vol. 20 No. 8 (2025): Special Issue
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v20i8.10558

Clostridioides difficile infection (CDI) is a major cause of nosocomial diarrhea worldwide, and limitations of standard antibiotic therapy have driven the adoption of fecal microbiota transplantation (FMT) as an alternative therapeutic approach. Although FMT achieves clinical success in most patients, treatment failure still occurs in a considerable proportion of cases. Existing literature has examined overall FMT efficacy, yet no review has comprehensively synthesized the multifactorial determinants of FMT failure while incorporating age-related considerations. This narrative review provides an integrative overview of factors contributing to FMT failure in adults with CDI. A literature search of PubMed and Google Scholar identified original research articles published between 2015 and 2025. Evidence indicates that FMT failure is influenced by host-related factors such as comorbidities, immune function, and nutritional status; disease-related characteristics, including CDI severity and recurrence history; technical aspects, such as bowel preparation, donor type, and route of administration; and pharmacological factors, including antibiotic exposure before or after the procedure. These factors collectively affect donor microbiota engraftment and increase the risk of therapeutic failure. Age-related physiological and microbiome changes may additionally modify treatment responses. This review highlights the need for comprehensive patient assessment, standardized procedural protocols, and careful post-FMT monitoring. Addressing current evidence gaps and improving clinical guidance will be essential for optimizing the safety and effectiveness of FMT across different adult age groups.

Narrative Review: The Effect of Inulin on the Growth of Bifidobacterium in the Human Intestine Nurzamani, Salsabila; Kasasiah, Ahsanal
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.10754

Bifidobacterium is an important probiotic bacterium that plays a key role in maintaining gut microbiota balance and supporting digestive health. Inulin has been widely studied as a prebiotic due to its ability to stimulate the growth of Bifidobacterium through fermentation in the colon. This study aims to summarize recent scientific evidence regarding the effects of inulin supplementation on the increase of Bifidobacterium populations in humans. This study aims to summarize recent scientific evidence regarding the effects of inulin supplementation on the increase of Bifidobacterium populations in humans. This article was prepared using a narrative review method based on literature searches conducted in PubMed, ScienceDirect, ClinicalKey, and Google Scholar. A total of nine original research articles that directly reported the effects of inulin supplementation were included. The findings consistently show that inulin increases the abundance of Bifidobacterium, improves gut microbiota balance, and enhances the production of short-chain fatty acids (SCFAs) such as acetate, butyrate, and propionate. These changes are also associated with improvements in metabolic markers among several populations, including obese children, prediabetic patients, and individuals with PCOS. Overall, inulin demonstrates strong bifidogenic effects, although individual responses may vary depending on dosage, duration of intervention, and physiological conditions.

Title Search

Found 50 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 50 Documents
Search