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All Journal PROSIDING SEMINAR NASIONAL BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Pro Food Jurnal Farmasi Sains dan Komunitas Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pengabdian Pada Masyarakat Indonesian Journal of Chemistry Jurnal Kesehatan Prima Gorontalo Journal of Public Health INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Majalah Ilmiah UPI YPTK Research Fair Unisri JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Buletin Pengabdian Prosiding Seminar Nasional Unimus
Stalis Norma Ethica
Universitas Muhammadiyah Semarang
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Secondary Metabolites Production of Bacillus spp. Isolated from Sea Cucumbers (Holothuria scabra) and their Activity against Mycobacterium smegmatis Maya Dian Rakhmawatie; Aida Zakiyatul Fikriyah; Ika Dyah Kurniati; Nanik Marfu'ati; Stalis Norma Ethica
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 20, No 2 (2023)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.004843

Abstract

New anti-tuberculosis agents are very important due to Multidrug-Resistant and Extensively Drug-Resistant Tuberculosis problems. Mycobacterium smegmatis can be used to replace Mycobacterium tuberculosis as bacteria test to increase the velocity of anti-tuberculosis screening. To answer the need for new drugs, exploration of secondary metabolites from Bacillus spp. can be conducted. Bacillus spp. are known to produce antimicrobials, including discovery of iturins, fengycins, and pumilacidins. This study explored the Bacillus spp. isolated from fermented intestines of Holothuria scabra. The production of Bacillus sp. Holothuria scabra Fermented Intestine (HSFI) secondary metabolites was done using culture media containing starch as a carbon source, as well as yeast and peptone as a nitrogen source. Production capacity of secondary metabolites of Bacillus sp. HSFI was calculated, to determine its potential as an antibacterial producer. Inhibition testing of secondary metabolites of Bacillus sp. HSFI against M. smegmatis was performed using the Kirby-Bauer disk diffusion method. Based on the results of the inhibition test, it was concluded that Bacillus sp. HSFI-9 has the greatest potential to inhibit the growth of M. smegmatis, with a moderate inhibition (7.67 mm). Production of secondary metabolites from Bacillus sp. HSFI-9 is exceptionally good with an extract production capacity of 24.6 mg/L.
Penguatan Pemahaman Arti Penting Pemeriksaan Tekanan dan Gula Darah Secara Rutin Bagi Masyarakat Meyliana, Adinda; Pratama, Aji Sukma; Janah, Inayah Qori Nur; Jimat, Resi Tondho; Sukeksi, Andri; Mukaromah, Ana Hidayati; Ethica, Stalis Norma
JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Vol 2 No 4 (2023): Oktober
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jipmi.v2i4.156

Abstract

Latar belakang: Prevalensi penyakit tidak menular termasuk tekanan darah tinggi dan Diabetes Mellitus (DM) terus meningkat di kalangan masyarakat. Namun, kesadaran dan pemahaman mengenai pentingnya pemeriksaan tekanan darah dan gula darah masih rendah di wilayah pedesaan, termasuk di Desa Tambakbulusan di Kabupaten Demak, Provinsi Jawa Tengah. Karena itu, peningkatan kesadaran tentang pentingnya pemeriksaan tekanan dan gula darah bagi masyarakat desa melalui penyuluhan relevan untuk dilakukan. Tujuan: Penyuluhan bertujuan meningkatkan kesadaran masyarakat Desa Tambakbulusan tentang pentingnya pemeriksaan kesehatan untuk menurunkan prevalensi penyakit hipertensi dan DM. Metode: Pelaksanaan kegiatan pengabdian kepada masyarakat dalam platform Pembangunan Kesehatan Masyarakat Desa (PKMD) diawali dengan identifikasi kebutuhan masyarakat akan penyuluhan tekanan darah tinggi dan DM melalui survei pendahuluan. Tahap perencanaan mencakup penentuan tujuan, materi penyuluhan, strategi penyampaian, lokasi, dan waktu kegiatan. Pra-pelaksanaan kegiatan meliputi pembuatan materi penyuluhan (poster). Dalam pelaksanaan kegiatan inti dilakukan diskusi, tanya jawab, studi kasus serta pembagian kuesioner pre- dan post-test. Kegiatan penyuluhan diakhiri dengan evaluasi untuk mendapatkan umpan balik dari peserta mengenai kegiatan yang telah dilakukan. Hasil: Setelah dilakukan kegiatan penyuluhan, pengetahuan masyarakat desa tentang penyakit tekanan darah dan DM meningkat berdasarkan skor pre-dan post-test, masing-masing dari rata-rata 61,7 menjadi 96,2 (55,6%) dan dari rata-rata 60,5 menjadi 95,4 (57,7%). Kesimpulan: Penyuluhan dengan media poster yang melibatkan pengisian kuesioner dapat meningkatkan kesadaran masyarakat Desa Tambakbulusan Kabupaten Demak tentang penyakit tekanan darah dan DM. Hasil penyuluhan ini perlu ditindaklanjuti dengan kegiatan pemeriksaan tekanan darah dan gula darah bagi masyarakat Desa Tambakbulusan. Kata kunci: Desa Tambakbulusan, diabetes mellitus, hipertensi, penyuluhan, poster ___________________________________________________________________________________________ Abstract Background: The prevalence of non-communicable diseases including high blood pressure and Diabetes Mellitus (DM) continues to increase in society. However, awareness and understanding of the importance of checking blood pressure and blood sugar is still low in rural areas, including Tambakbulusan Village in Demak District, Central Java Province. Therefore, increasing awareness about the importance of checking blood pressure and sugar in rural communities through counseling is relevant to do. Objective: Counseling aims to increase the awareness of the Tambakbulusan Village community about the importance of health checks to reduce the prevalence of hypertension and DM. Method: The implementation of community service activities in the Village Community Health Development (PKMD) platform was initiated by identifying community needs for counseling on high blood pressure and DM through a preliminary survey. The planning stage included determining objectives, extension materials, delivery strategy, location, and time of activity. Pre-implementation of activities included making counseling materials (posters). In the core activities discussions, questions and answers, case studies, and distribution of pre-and post-test questionnaires were carried out. The counseling activity ended with an evaluation to get feedback from the participants regarding the activities that had been carried out. Result: After the counseling activities were carried out, the knowledge of the villagers about blood pressure and DM increased based on the pre-and post-test scores, respectively from an average of 61.7 to 96.2 (55.6%) and from an average of 60.5 to 95.4 (57.7%). Conclusion: Counseling with posters involving the use of questionnaires can increase the awareness of the people of Tambakbulusan Village, Demak Regency about blood pressure and DM. The results of this counseling need to be followed up with activities to check blood pressure and blood sugar for the people of Tambakbulusan Village.  Keywords: Tambakbulusan Village, diabetes mellitus, hypertension, counseling, posters
Penyuluhan dan Pemeriksaan Golongan Darah Sistem ABO - Rhesus Bagi Anak-Anak dan Pengelola Panti Asuhan Sonaf Maneka Kupang, Nusa Tenggara Timur Musa, Susan Hasanah; Mutmainnah, Siti; Ayanti, Bio Putri; Dewi, Okta Yosiana; Ernanto, Aditya Rahman; Sultistyaningtyas, Ayu Rahmawati; Ethica, Stalis Norma; Afriansyah, Muhammad Ardi
JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Vol 3 No 1 (2024): Januari
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jipmi.v3i1.294

Abstract

Latar belakang: Golongan darah merupakan informasi yang penting bagi setiap individu, karena menentukan berbagai aspek medis seseorang yang meliputi antara lain keperluan transfusi darah, transplantasi organ dan kehamilan. Keberhasilan dalam berbagai aspek medis tersebut sangat ditentukan oleh kecocokan golongan darah. Hasil survei pendahuluan menunjukkan bahwa Anak-anak dan pengelola  Panti Asuhan Katolik Sonaf Maneka Kupang  banyak yang belum mengetahui manfaat mengetahui golongan darah dan belum memiliki informasi tentang golongan darahnya masing-masing. Tujuan: memberikan pengetahuan kepada anak-anak dan pengelola panti Asuhan Sonaf Manek di Kupang tentang pentingnya mengetahui golongan darah dan memberikan informasi tentang golongan darahnya masing-masing. Metode: Penyuluhan dilakukan oleh tim mahasiswa dan dosen Universitas Muhammadiyah dalam skema Pembangunan Kesehatan Masyarakat Desa (PKMD) dengan metode ceramah yang didukung penggunaan media poster. Evaluasi kegiatan penyuluhan dilakukan dengan kuesioner, sedangkan pemeriksaan golongan darah sistem ABO (A, B, AB dan O) dan Rhesus dilakukan secara langsung oleh mahasiswa Program Diploma Teknologi Laboratorium Medik. Hasil: Hasil analisis yang dilakukan menunjukkan bahwa kegiatan penyuluhan dengan media poster meningkatkan pemahaman peserta akan pentingnya pengetahuan tentang golongan darah. Kesimpulan: hasil pemeriksaan golongan darah menunjukkan bahwa anak-anak dan pengelola panti asuhan yang bergolongan darah A, B, AB, O berturut-turut 23, 43, 7, dan 27% serta seluruhnya memiliki golongan darah Rhesus Positif (Rh+). Kata kunci: media poster, panti asuhan, pemeriksaan golongan darah, pembangunan kesehatan masyarakat desa _______________________________________________________________________________ Abstract Background: Blood type is important information for every individual, as it determines various aspects of a person's medical such as the need for blood transfusions, organ transplants, and pregnancy. The compatibility of blood types largely determines success in various medical aspects. The preliminary survey results show that many children and tenants at the Sonaf Maneka Catholic Orphanage in Kupang did not know the benefits of knowing their blood type and did not yet have information about their respective blood groups. Objective: to provide knowledge to the children of the Sonaf Maneka Orphanage at Kupang about the importance of knowing their blood type and providing information about their respective blood groups. Method: Counseling was carried out by a team of students and lecturers of Muhammadiyah University in the Village Community Health Development (PKMD) scheme with the lecture method supported by media posters. Evaluation of activities carried out by questionnaires, ABO, and Rhesus system blood group examinations were carried out directly by students of the Diploma Program of Medical Laboratory Technology. Result: Results of the analysis showed that the outreach activities with posters increased participants' understanding of the importance of knowledge about blood groups. Conclusion: the results of the blood group examination showed that orphanage tenants with blood types A, B, AB, and O were 23, 43, 7, and 27% respectively and all were Rh-Positive (Rh+). Keywords: poster media, orphanage children, counseling, blood type test, village community health development
In Vitro Anticoagulant Activity of Crude Protease of Bacillus tequilensis HSFI-5 Ethica, Stalis Norma; Raharjo, Tri Joko; Zilda, Dewi Seswita; Hidayati, Nur
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.3791

Abstract

obtained from the fermented intestine of Holothuria scabra (sand sea cucumber). Strain HSFI-5 had been reported to be able to produce proteases, which had shown several characteristics of an antithrombotic agent, i.e., fibrinolytic and clot-lysis activities. However, its anticoagulation activity test is yest to be done. This study aimed to determine the anticoagulant activity of the crude protease HSFI-5 in vitro. The study design was a completely randomized design with a sample size of 90 calculated using the Federer formula. The material used was crude protease from B. tequilensis in skim milk broth. Prothrombin time (PT), activated partial thromboplastin time (aPTT), and plasma recalcification time (PRT) were carried out to test the anticoagulant activity. Citrated platelet poor plasma samples were divided into positive control, normal control, direct examination with crude enzyme in volumes of 50 and 100 µL and pre-incubation at 37ºC for 5, 10, and 15 min with crude enzyme volumes of 50 and 100 µL. The data normality was tested with the Kolmogorov-Smirnov test and the different tests were analyzed by one-way ANOVA with the Post hoc LSD test. The results of one-way ANOVA both on PT, aPTT, and PRT examinations showed that there was a significant difference between the treatment groups (p<0.05). The longest results of PT, aPTT, and PRT are positive controls, and the shortest results are normal controls for PT, and 15’ 50 group for aPTT and PRT. It is clear that crude protease B. tequilensis HSFI-5 exhibits anticoagulant as well as thrombolytic action, raising the possibility that it could function as an antithrombotic drug.
Improvement of Awareness of Diabetes Mellitus Disease Risks and Self-Monitoring Motivation Through Blood Sugar Screening and Counseling for Dian Darat Village Community, Southeast Maluku Meturan, Dessy Marselina; Ethica, Stalis Norma; Sukeksi, Andri
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 10, No 1 (2024): March
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.79774

Abstract

Diabetes Mellitus (DM) disease is among the top 10 causes of death in the world. It is a metabolic disease causing unusual high blood sugar level in the body. Fortunately, controlling risk factors through early detection can prevent the abnormality. Dian Darat is among villages in Southeast Maluku Regency, where DM screening is rarely carried due to its relatively remote location. This community activity aimed to provide DM disease education through blood sugar screening and counselling for village’s community. Methods included early detection of DM and counselling based on blood sugar level screening results supported by poster media. Screening test results showed that 9 out of 26 respondents (35%) had blood sugar level higher than normal. Based on results of pre- and post-test scores before and after the education, there was an increase in participants’ knowledge regarding DM disease by 62 %. (from average score of 56,15 ± 13,88 to 90,77 ± 9,77, n=26, p ≤ 0.01). Future community services are recommended on similar and more sustainable community service activities by cooperation with other relevant stakeholders to optimize results.
Antimicrobial ointment based on Bacillus subtilis subsp. subtilis HSFI-9 isolated from Sea Cucumber of Kodek Bay Lombok Indonesia Rakhmawatie, Maya Dian; Diatri, Devita; Samiroh, Samiroh; Abdillah, Rifqi; Arfiyanti, Mega Pandu; Ethica, Stalis Norma; Zilda, Dewi Seswita
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 20, No 1 (2025): May 2025
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.925

Abstract

The ethyl acetate extract of Bacillus subtilis subsp. subtilis HSFI-9, a bacteria isolated from intestinal fermentation of sea cucumbers (Holothuria scabra) is known to have antimicrobial properties. This research aimed to determine the activity of HSFI-9 extract ointment against Staphylococcus aureus and Candida albicans. Extract ointment is a topical preparation for skin infections made from a mixture of Vaseline Alba and Adeps lanae bases. The extract ointment was prepared into four concentrations of 0.003%, 0.03%, 0.3%, and 1% w/v using HSFI-9 as active ingredient.  Antimicrobial assay was carried out in vitro using the disc diffusion method. The extract ointment was evaluated based on organoleptic, homogeneity, spreadability, adhesion characteristics, and pH tests. The optimal concentration of the extract ointment to inhibit the growth of S. aureus  0.3% (inhibition zone of 11.67±1.26 mm) and against C. albicans is 0.03% (inhibition zone of 10.16±1.50 mm). The activity of the extract ointment was categorized as strong although not as strong as the antibiotic control ointment Mupirocin 2% or Ketoconazole 2%. The extract ointment organoleptic indicated a characteristic odor of ethyl acetate, was yellowish-white ointment, and had a homogeneous and smooth consistency. The extract ointment also had properties such as good spreadability but poor adhesion and tended to have an acidic pH ( 4.5). The HSFI-9 extract can be declared feasible for the development of topical antimicrobials. The ointment still needs to be optimized, especially regarding improving the adhesion characteristic and pH to be safe for the skin and mucosa.
Potential of Crude Protease of Bacillus Sp. HSFI-9 ss Anticoagulant Agent and Meat Tenderizer Izza, Naimatul; Saptaningtyas, Ragil; Stalis Norma Ethica; Kartika, Aprilia Indra
Jurnal Kesehatan Prima Vol. 19 No. 2 (2025): AUGUST
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jkp.v19i2.1663

Abstract

Some proteases act as thrombolytic enzymes to destroy the abnormal blood clot in the body called a thrombus, a leading cause of mortality associated with cardiovascular disease (CVD). In addition, the enzyme may also hydrolyze the meat’s proteins into simple amino acids, causing the meat to become tender. The study's aim was to analyze the potential of the crude protease of Bacillus sp. HSFI-9 is an anticoagulant agent and meat tenderizer. The anticoagulant test of crude protease HSFI-9 on venous blood samples was performed by measuring the clotting time compared to control (10% EDTA) by the Lee-White method. Protein profiles of beef, chicken, and tuna were analyzed before and after immersion in the crude protease of Bacillus sp. HSFI-9 in concentration of 30% v/v for 3 hours based on Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. The anticoagulant test screening showed that the crude protease of Bacillus sp.  HSFI-9 was able to prolong the blood clotting time even though its anticoagulant activity was still less than that of 10% EDTA (a commercial anticoagulant agent used as a positive control). The SDS-PAGE results indicated that soaked beef and chicken using crude protease from Bacillus sp. HSFI-9 could hydrolyze the meats’ proteins by denaturing them into smaller molecules, indicated by the appearance of new minor protein bands and the disappearance of major ones. It can be concluded that the crude protease of Bacillus sp. has potential as an anticoagulant agent and a meat tenderizer.
Co-Authors A.A. Ketut Agung Cahyawan W Abdillah, Rifqi Aditya Rahman Ernanto Aditya Rahman Ernanto Aditya Rahman Ernanto Afriansyah, Muhammad Ardi Agus Sabdono Agus Suyanto Aida Zakiyatul Fikriyah Ainutajriani Ainutajriani Alisha Triwahyuni Alvira Intar Bella Cahya Ana Hidayanti Mukaromah Ana Hidayati Mukaromah Ana Hidayati Mukaromah Andri Sukeksi Aprianti, Nurannisa Fitria Aprilia Indra Kartika, Aprilia Indra Arfiyanti, Mega Pandu Aufit Fahima Aulia Harun Aulia Meirizka Saputra Ayanti, Bio Putri Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Chaerul Arham Dewi Seswita Zilda Dewi, Okta Yosiana Dhea Ayu Lestari Dian Purwo Jatinging Putri Diatri, Devita Dilin Rahayu Nataningtyas Dwi Pamaya Elly Rustanti Ellyka Purwaningrum Endang Semiarti Endang Semiarti Endang Tri Wahyuni Maharani Ernanto, Aditya Rahman Fanda Indriyani Fandhi Adi Wardoyo Feri Feri Fuad, Hayatun Hayatun Fuad Ika Dyah Kurniati Istini Istini Izza, Naimatul JAKA WIDADA Jaka Widada Janah, Inayah Qori Nur Jimat, Resi Tondho Kazi Mohammad Zillur Rahman L. Hartanto Nugroho Maya Dian Rakhmawatie Maya Linda Shafira Meturan, Dessy Marselina Meyliana, Adinda Mudyawati Kamaruddin Muhammad Ardi Afriansyah Muhammad Ardi Afriansyah Mukarromah, Ana Hidayanti Musa, Susan Hasanah Mutia Srikandi Fitria Nanik Marfu&#039;ati Noverson Lidaya Nur Hidayah Nur Hidayah Nur Hidayati Nur Hidayati Nurcahyono Nurcahyono Nurrahman Nurrahman Nurrahman Nurrahman Oedjijono Oedjijono Pratama, Aji Sukma Puji Lestari Radna Safitri Ragil Saptaningtyas Ragil Saptaningtyas Ragil Saptaningtyas, Ragil Richard David Silvestre Rivana Ariyadi Rosyida Azis Rizki Sakti Imam Muchlissin Samiroh Samiroh, Samiroh Setia Iriyanto Silvestre, Richard David Siska Nurprihandayani Siti Aminah Siti mutmainnah Sofyan Yosias Beslar Sri Darmawati Sri Darmawati Sri Darmawati Sri Elvira Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi suardi suardi Sukowiyono Sukowiyono Sulistyaningtyas, Ayu Rahmawati Sultistyaningtyas, Ayu Rahmawati Theresia Liyana Sejati Tri Joko Raharjo Tri Joko Raharjo Vicky Mahendra Nurzhulian Vradea Pramesta Wa Ode Inayatul Wikanastri Hersoelistyorini Wikanastri Hersoelistyorini Yuni Nurkuntari