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All Journal HAYATI Journal of Biosciences Teknosains: Media Informasi Sains dan Teknologi Folia Medica Indonesiana Biology, Medicine, & Natural Product Chemistry Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Penelitian Pendidikan IPA (JPPIPA) EKONOMIS : Journal of Economics and Business Majalah Farmaseutik JMM (Jurnal Masyarakat Mandiri) SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Indonesian Journal of Biology Education Jurnal Kreativitas PKM Care : Jurnal Ilmiah Ilmu Kesehatan JPBIO (Jurnal Pendidikan Biologi) Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Community Development Journal: Jurnal Pengabdian Masyarakat Indonesia Berdaya Nusantara Science and Technology Proceedings Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat MAHESA : Malahayati Health Student Journal Jurnal Abdimas Sangkabira Molucca Medica Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan Jurnal PADE: Pengabdian & Edukasi
Melda Yunita
Departemen Mikrobiologi Fakultas Kedokteran, Universitas Pattimura
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Journal : JPBIO (Jurnal Pendidikan Biologi)

 1 
Isolation of endophytic bacteria from nutmeg plant as antibacterial agents against pathogenic bacteria Melda Yunita; Yosephina Manse; Myllisa Citra Wulandari; Sukmawati Sukmawati; Morgan Ohiwal
JPBIO (Jurnal Pendidikan Biologi) Vol 7, No 1 (2022): April 2022
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v7i1.1522

Abstract

 Recently, exploration of natural antibiotic alternatives by utilizing medicinal plants is often carried out. Nutmeg plant (Myristica fragrans Houtt) is a tropical plant endemic to the Maluku Island and often used as medicine by local people. This study was aimed to isolate endophytic bacteria from the nutmeg plant and detect its potential as an antibacterial against pathogenic bacteria. The study is a descriptive observational study with a true experimental approach. Endophytic bacteria were isolated from several organs of the nutmeg plant such as leaves, leaf bones, stems, fruit flesh, and seeds and then tested for their inhibition against Staphylococcus aureus, Propionibacterium acne, and Escherichia coli. The results showed that: (1) the highest total population of endophytic bacteria was found in the seeds of nutmeg (7.5x104 CFU/ml), (2) ten isolates of endophytic bacteria were obtained with varying morphological characteristics, and (3) the detection of antibacterial activity showed that of the 10 isolates obtained, 4 isolates were able to inhibit the growth of Escherichia coli, namely isolate BEAD2, BEATD1, BEABJ 2, and BEABJ 1 with the diameter of the inhibition zones of each isolate being 3.4 mm, 3.2 mm, 1.6 mm and 1.5 mm respectively. The conclusion of this study is that 4 isolates of endophytic bacteria were detected as potential antibacterial against Escherichia coli.
The measurement of indole acetic acid from rhizosphere bacteria Sukmawati Sukmawati; Nurul Kusuma Dewi; Melda Yunita
JPBIO (Jurnal Pendidikan Biologi) Vol 6, No 1 (2021): April 2021
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v6i1.872

Abstract

In Indonesia, synthetic chemical fertilizers are generally used to spur plant growth. The use of synthetic chemical fertilizers is known to reduce soil quality, causing resistance to pests and plant diseases. One of the natural potencies that can be used as growth promoters is Indole Acetic Acid (IAA) producing bacteria. The objective of this study was to measure the levels of Indole Acetic Acid (IAA) from the rhizosphere bacterial isolate of green beans which is thought to be able to produce Indole Acetic Acid (IAA). The method used was a descriptive approach, including the isolation of green bean rhizosphere bacteria, measurement of Indole Acetic Acid (IAA) levels in bacterial isolate, and data analysis. Green bean rhizosphere bacterial isolate produced Indole Acetic Acid (IAA) with an indication of a color change after the addition of the Salkowski reagent, and quantitatively the results of calculations in the standard curve equation for Indole Acetic Acid (IAA) obtained a value of Indole Acetic Acid (IAA) levels was 50.91 ppm.Keywords: Indole acetic acid, rhizosphere, bacteria
Analysis of escherichia coli dan salmonella sp. in shrimp fermentation products Sukmawati Sukmawati; Melda Yunita; Sipriyadi Sipriyadi; Erin D Noya; Henok Siboka Onim
JPBIO (Jurnal Pendidikan Biologi) Vol 7, No 2 (2022): November 2022
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v7i2.1785

Abstract

South Sorong, West Papua, is one of the areas that produce rebon shrimp. Rebon shrimp can be made into fermented products such as rebon shrimp paste (terasi) or acidic rebon shrimp paste (cincalok). Terasi is a food additive that functions as a seasoning or flavoring, while cincalok is a fermented food added with salt and rice. The objective of this study was to determine the contamination of E. coli and Salmonella in fermented rebon shrimp products. This study was a descriptive study by describing the presence or absence of contamination by E. coli and Salmonella sp. on rebon shrimp paste (terasi) and acidic rebon shrimp paste (cincalok). The study concluded that the samples of rebon shrimp paste (terasi) and acidic rebon shrimp paste (cincalok) in South Sorong were safe from contamination by Escherichia coli and Salmonella sp. According to the Indonesian National Standards Agency, fermented fish products with or without salt, good and suitable products for consumption are those that are negative for Salmonella, V. cholera, Escherichia coli with a limit of <3 MPN/g and Staphylococcus aureus should be <1 x103 colonies/g. 
Antibiotic susceptibility analysis of Escherichia coli as the cause of urinary tract infection Melda Yunita; Hasna Vuspita Latuconsina; Eka Astuty; Alessandra Flowrence Saija
JPBIO (Jurnal Pendidikan Biologi) Vol 7, No 2 (2022): November 2022
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v7i2.1849

Abstract

The incidence of urinary tract infection (UTI) in Indonesia is still quite high. The UTIs are generally treated with antibiotic. However, the use of antibiotics has recently increased tremendously thus triggering bacterial resistance. This study was to analyze the susceptibility of the Escherichia coli as the cause of the UTI to 15 antibiotics at the UPTD Health Laboratory Center of Maluku Province. The study was an experimental laboratory study using the disk diffusion method. The antibiotic disc was placed on the media and the inhibition zone against E. coli was measured. The results showed that the E. coli was resistant (26.67%) to 4 types of antibiotics, namely amoxilin, ampicillin, sulfametazole dan penicillin. Meanwhile, E. coli was sensitive (46.66%) to 7 types of antibiotics, namely kanamycin, gentamicin, subactam ampicillin, chloramphenicol, amikacin, ceffazedine and azithromycin. Furthermore, E. coli was intermediate (26.67%) against 4 types of antibiotics, namely ciprofloxacin, ceptriaxone, cefoxitin and colistin. This study concluded that of the 15 antibiotics tested, gentamicin, amikacin, and kanamycin were the most effective antibiotics in inhibiting the growth of E. coli that causes UTI. Nevertheless, the appropriate choice of antibiotic needs to be adjusted only by physicians based on local, regional and national patterns of susceptibility.
The potential of endophytic bacteria isolated from atung fruit seeds as antibacterial against skin pathogens Melda Yunita; Adelia Mantong; Halidah Rahawarin
JPBIO (Jurnal Pendidikan Biologi) Vol 8, No 1 (2023): April 2023
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v8i1.2271

Abstract

Antibiotic resistance is one of the biggest threats to global health today. Estimated by 2050, antibiotic resistance will cause around 10 million deaths per year if alternative treatments have not been identified. This study was aimed to determine the potential of endophytic bacterial isolates from the seeds of atung fruit (Parinarium glaberrimum Hassk) as an antibacterial against skin pathogens Cutibacterium acnes and MDR Staphylococcus aureus, and Staphylococcus epidermidis. This research was a descriptive observational research with laboratory experimental methods. This study used isolates of endophytic bacteria isolated from the seeds of atung fruit which were tested with pathogenic bacteria. The results showed that 2 of 7 endophytic bacterial isolates were able to inhibit the growth of C. acnes with clear zone diameter of 4 mm and 9 mm, respectively, yet couldn’t able to inhibit MDR S. aureus and S. epidermidis. This study concluded that the 2 isolates (isolate BBA5 and BBA9) could have potential as antibacterial against C. acnes. While 5 other isolates had not been able to inhibit the whole pathogenic bacteria tested. An optimization is necessary in an effort to develop these isolates as antibacterial.
Biochemical identification of endophytic bacteria isolates from medicinal plant kayu jawa lannea coromandelica (houtt.) merr Eka Astuty; Indas Wari Rahman; Melda Yunita
JPBIO (Jurnal Pendidikan Biologi) Vol 8, No 1 (2023): April 2023
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v8i1.2095

Abstract

Medicinal plants and their endophytes are a source of valuable bioactive compounds and their secondary metabolites which contribute to more than 80% of natural medicines available in the market. A plant that has long been used as a traditional medicinal herb for healing is the Kayu Jawa (Lannea coromandelica (Houtt) Merr). This study aimed to characterization and identification of endophytic bacterial isolates isolated from medicinal plants Kayu Jawa (L. coromandelica (Houtt.) Merr). This research is a laboratory experimental research. In total, 8 isolates were selected as representatives of the bacterial community living in the tissues of Kayu Jawa (L. coromandelica (Houtt.) Merr). The microscopic observation was performed by Gram staining. Biochemical identification was conducted using Vitek 2. The results showed that Bacillus was the dominant genus in this study followed by Staphylococcus and Pantoea. Based on the research that has been done, it can be concluded that the endophytic bacteria isolated from Kayu Jawa Lannea coromandelica (Houtt) Merr, six isolates belonging to Bacillus and another two belonging to Staphylococcus and Pantoea genera. However, for the exploration of this prospective application, it is very important to do a systematic study of the biological and distribution of endophytic bacteria in the host.
Microbiological quality analysis of grouper salted fish (Epinephelus Fuscoguttatus) in several traditional markets in ambon Talakua, Gratia; Yunita, Melda; Husein, Anggun Lestary
JPBIO (Jurnal Pendidikan Biologi) Vol 9, No 2 (2024): November 2024
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v9i2.3709

Abstract

Salted fish is one of the food ingredients that is in demand by the community and has great potential in almost all traditional markets in Maluku, especially in the city of Ambon. One of the salted fish is grouper fish (Epinephelus fuscoguttatus) great potential, so it is necessary to conduct food safety checks from the microbiological aspect. This study aimed to analyze the total plate count of bacteria in grouper salted fish sold in several traditional markets in Ambon City, Indonesia. The research method used was quantitative descriptive with an experimental laboratory approach. The results of the total bacterial plate in the grouper salted fish sample from the Mardika market ranged from 1,55 x 103 CFU/mL to 1,05 x 104 CFU/mL, the origin of the Rumah Tiga market ranged from 3,85 x 103 to 3,5 x 104 and the origin of the Waiheru market ranged from 9,5 x 102 to 4,5 x 103 CFU/mL. In conclusion, the three samples of grouper salted fish do not exceed the maximum limit of the total plate count according to the Indonesian National Standard (INS) of 1x105 CFU/g so considered safe for consumption from a microbiological aspect.
Antibacterial activity in vitro investigation of eucheuma cottonii extract from aru islands against pathogenic bacteria Yunita, Melda; Warella, Juen Carla; Astuty, Eka; Ohiwal, Morgan; Alimudi, Saiful
JPBIO (Jurnal Pendidikan Biologi) Vol 9, No 1 (2024): April 2024
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v9i1.3241

Abstract

Red algae Eucheuma cottonii is one of the potential marine biodiversity that provides added value in the pharmaceutical and cosmetic fields and is known to have antibacterial compounds. Research focusing on the potential of E. cottonii as an antibacterial is still limited, particularly when it comes to the Aru Islands. This study aimed to measure the antibacterial activity of E. cottonii against pathogenic bacteria including E. coli and S. aereus at several concentrations and to determine the best concentration for inhibiting these two pathogenic bacteria. The study was true experimental laboratory research with a post-test-only controlled group design. Antibacterial tests using the disc diffusion method and phytochemical tests were carried out. This study revealed that E. cottonii could be able to inhibit S. aureus ranging from 0.27±0.03 – 2.1±0.14 at a concentration of 50%-100%, and 0.17±0.05 – 0.45 ± 0.03 against E. coli at a concentration of 80-100%. Meanwhile, saponins and flavonoids were two bioactive compounds found through phytochemical testing. The study concluded that the n-hexane extract of E. cottonii was able to inhibit the growth of E. coli and S. aureus, even with a weak inhibition category. This study recommends that improvements are needed in the E. cottonii extraction process to maximize the antibacterial properties of E. cottonii.
Isolation and characterization of phosphate-solubilizing bacteria in land managed with the dusung system Ohiwal, Morgan; Marasabessy, Rendy; Yunita, Melda; Sukmawati, Sukmawati; Qibtiyah, Mariyatul
JPBIO (Jurnal Pendidikan Biologi) Vol 10, No 2 (2025): November 2025
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v10i2.4821

Abstract

Soil microorganisms play a crucial role in nutrient cycling and soil fertility, particularly through their ability to solubilize insoluble phosphate compounds. This study aims to isolate and characterize phosphate-solubilizing bacteria (PSB) on land managed under the dusung agroforestry system. Soil samples were collected from five types of land use: forests, mixed orchards, clove hamlets, nutmeg orchards, and shrubs. PSB isolation was performed using Pikovskaya medium with the spread plate method, followed by characterization of colony morphology (shape, edge, color, elevation) and Gram staining. The results showed that the highest PSB population occurred in forest land (8.3 × 10? CFU/ml), followed by mixed orchards (6.2–6.7 × 10? CFU/ml), clove orchards (5.3–5.4 × 10? CFU/ml), nutmeg orchards (3.2–5.6 × 10? CFU/ml), and the lowest in shrubs (7.1 × 10³ CFU/ml). The characteristics of PSB varied, with isolates from forests and nutmeg orchards predominantly consisting of coccus-shaped Gram-positive bacteria, while those from mixed and clove orchards were dominated by bacillus-shaped Gram-positive bacteria. The population and diversity of PSBs were influenced by the availability of organic matter, soil pH, and land management practices. These findings highlight the ecological importance of the dusung system in sustaining soil fertility and promoting ecosystem resilience.
Co-Authors Abbas, Muthmainnah Ade Nurhayati Kusumadewi Adelia Mantong Alessandra Flowrence Saija Alimudi, Saiful Anggun Lestari Husein Astuti Nur Fadhilah ASTUTI, EKA Astuty, Eka Augie Desvie Klausya Kadun B. Bension, Johan Bandjar, Fitri Kadarsih Bandjar, Fitri Kardasih Cucu Hodijah de Lima, Filda V.I. de Lima, Filda Vionita Irene Dilya Maghfirah Taher DRIANA, Elin Dylan Tamalsir Dylan Tamalsir Effendi Effendi Eka Astuti Eka Astuty Elpira Asmin Elpira Asmin Elpira Asmin Erin D Noya Ernawati Ernawati Esmeraldine, Genevieva Etrin Zulqarnain Eka Saputra Linggar Evangelista Risalia Haurissa Fadila Tri Starlia Farah Christina Noya Filda de Lima Grace Latuheru Halidah Rahawarin Hartono Nurlette Hasibuan, Andrian Bayo Arif Hasna Vuspita Latuconsina Hataul, Is Asma’ul Haq Henok Siboka Onim Hursepuny, Valentine Husein, Anggun Lestary Ilsan, Noor Andryan Indas Wari Rahman Inggrain, Maulin Inggraini, Maulin Intania Riska Putrie, Intania Riska Irwan Ivanmorl Ruspanah Jennifer Vivian Kiriwenno Jepri Agung Priyanto, Jepri Agung Johan B Bension Johan Bruyif Bension Kusnara, Hasti Pramesti Latuheru, Grace Lesbatta, Kezia Josawel Mailoa, Joice Mainase, Josepina Makatita, Miftah H Marasabessy, Rendi Marasabessy, Rendy Mariyatul Qibtiyah Maxwell Landri Vers Malakauseya Metekohy, Meilani Mus, Rosdiana Musaid, Hendra Mutmainnah Abbas Myllisa Citra Wulandari Nahumarury, Salmin Nahumarury, Salmin Safril Noviyanter Siahaya Noya, Farah C Nurdin, Sukmawati Nurul Kusuma Dewi Ohiwal, Morgan Patteilohy, Merry Presly G. Siahaya Rachmawati Dwi Agustin Rahawarin, Halidah Ramanda, Viqih Ramla, Razita Ratau, Asria Reubun, Ririn Jihan S Rosalina, Febrianti Rumatiga, Masdar Ruth Magdalena Lumbantobing Safinah Yulianty Sitania Sipriyadi Siti Nur Azizah Siti Nurfajriah Sofia Mustamu Soumena, Rifah Zafarani Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati, Sukmawati Sulfiana Sulfiana SULFIANA, SULFIANA Tahitu, Ritha Talakua, Gratia Tamtelatihu, Carmila L Titin Agustina Titin Agustina Umban Adi Jaya Usman Umarella Valentine Hursepunny Victor Leo Nolly Kewilaa Vina Z. Latuconsina Warella, Juen Carla Yensy Lolita Salamor Yosephina Manse Yuliawati , Sri Yuniasih Mulyani Jubeliene Taihuttu