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All Journal HAYATI Journal of Biosciences Teknosains: Media Informasi Sains dan Teknologi Folia Medica Indonesiana Biology, Medicine, & Natural Product Chemistry Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Penelitian Pendidikan IPA (JPPIPA) EKONOMIS : Journal of Economics and Business Majalah Farmaseutik JMM (Jurnal Masyarakat Mandiri) SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Indonesian Journal of Biology Education Jurnal Kreativitas PKM Care : Jurnal Ilmiah Ilmu Kesehatan JPBIO (Jurnal Pendidikan Biologi) Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Community Development Journal: Jurnal Pengabdian Masyarakat Indonesia Berdaya Nusantara Science and Technology Proceedings Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat MAHESA : Malahayati Health Student Journal Jurnal Abdimas Sangkabira Molucca Medica Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan Jurnal PADE: Pengabdian & Edukasi
Melda Yunita
Departemen Mikrobiologi Fakultas Kedokteran, Universitas Pattimura
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Journal : Folia Medica Indonesiana

 1 
Antifungal Activity of Endophytic Bacteria Isolated from Miana Plants (Coleus scutellarioides (L.) Benth.) against Candida albicans Melda Yunita; Ruth Magdalena Lumbantobing; Ritha Tahitu
Folia Medica Indonesiana Vol. 59 No. 3 (2023): September
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v59i3.43989

Abstract

Highlights: 1.Due to the adverse effects associated with current antifungal drugs, research on traditional medicine is necessary to explore other options for candidiasis treatment.2.Following the findings of this study, it is recommended to conduct further research by incorporating Coleus scuttellarioides filtrate into the growth medium, since this may improve bacterial growth by producing optimal secondary metabolites.   Abstract Candida albicans is the most common organism responsible for both mucosal and systemic infections, accounting for approximately 70% of fungal infections worldwide. Miana, scientifically known as Coleus scuttellarioides (L.) Benth., is recognized for its use in traditional medicinal practices. Miana plants contain endophytic bacteria that possess the ability to produce secondary metabolites with potential antifungal agents. The objective of this study was to assess the antifungal activity of nine endophytic bacteria isolates derived from Coleus scuttellarioides against Candida albicans. This study was laboratory-based qualitative experimental research that applied the Kirby-Bauer diffusion method and several modifications. The Candida albicans specimens were spread throughout the entire potato dextrose agar medium. Afterwards, paper discs that had been soaked in a liquid culture of endophytic bacterial isolates were carefully placed on the surface of the medium. The complete setup was then incubated for 1–2 days. The potential antifungal activity of endophytic bacteria was assessed by observing the emergence of a clear zone surrounding their growth, which would indicate inhibition. An additional observation was performed in the follow-up test, involving the use of Sabouraud dextrose agar medium to confirm the initial test result. The results from the inhibitory test revealed that none of the bacterial isolates exhibited any inhibition zone. Conversely, ketoconazole as the positive control showed an inhibition zone with an average diameter of 28.5 mm. In conclusion, endophytic bacterial isolates obtained from Coleus scuttellarioides have no discernible antifungal properties against Candida albicans. This study implies that ketoconazole remains effective in treating infections caused by Candida albicans.
Total Plate Count Analysis and Food-Contaminating Bacterial Identification of Smoked Tatihu (Thunnus albacares) Sold in Several Traditional Markets in Ambon, Indonesia Evangelista Risalia Haurissa; Melda Yunita; Sulfiana, Sulfiana
Folia Medica Indonesiana Vol. 60 No. 3 (2024): September
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v60i3.62231

Abstract

Highlights:1. This research provides important information regarding the food safety of smoked tatihu (yellowfin tuna) sold in several traditional markets in Ambon, Indonesia.2. We find that smoked tatihu fish samples examined at 2 of 3 traditional markets in Ambon city are unsafe for consumption according to the Indonesian National Standard due to bacterial contamination.3. The findings indicate that smoked fish can be contaminated by Gram-positive bacteria, such as Staphylococcus gallinarum, Staphylococcus sciuri, Rothia kristinae, and Staphylococcus pseudintermedius.   Abstract The large marine area and abundant fish resources of Maluku Province, Indonesia, are in contrast to the poor hygiene of its traditional markets, which can cause microbial contamination and taint processed products, such as smoked tatihu (yellowfin tuna). In Ambon city, Maluku, Indonesia, no research had been conducted concerning total plate count analysis and food-contaminating bacterial identification that could guarantee the microbiological safety of smoked tatihu. Therefore, this study aimed to assess the microbiological quality of smoked tatihu according to Indonesian National Standards (INS 2725:2013) and to identify any presence of food-contaminating bacteria. This research was a quantitative descriptive study with a true experimental laboratory approach. The samples used were smoked tatihu collected from three traditional markets in Ambon, Indonesia. The spread plate method was used in the isolation process, while the total plate count analysis was performed to estimate the quantity of colonies on each petri dish. Bacterial identification was carried out macroscopically and microscopically. The microscopic identification involved Gram staining to determine the shape and color of the bacteria. Additionally, the bioMérieux VITEK 2 Compact system was utilized for biochemical identification to ascertain the species of bacteria present. The results revealed that the colony counts in smoked tatihu from the Mardika market and Hative Kecil market were 1.1 x 104 CFU/g and 8.2 x 106 CFU/g, respectively. However, smoked tatihu from the Batu Meja market had an excessive number of colonies that were difficult to quantify. The contaminating bacteria were identified as Staphylococcus gallinarum, Staphylococcus sciuri, Rothia kristinae, and Staphylococcus pseudintermedius. In conclusion, smoked tatihu fish from the Mardika market are considered safe for consumption as the microbiological parameters do not exceed the Indonesian National Standards, whereas those obtained from the Hative Kecil and Batu Meja markets are unsafe for consumption due to the excessive presence of food-contaminating bacteria.
Assessment and Identification of Bacteria in Sagu Lempeng Sold in Ambon City, Indonesia Dilya Maghfirah Taher; Melda Yunita; Yuniasih Mulyani Jubeliene Taihuttu; Siti Nur Azizah
Folia Medica Indonesiana ON PROGRESS
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Highlights:1. This study is the first to analyze the microbiological quality of sagu lempeng in traditional markets around Ambon, Indonesia, providing a new reference for the food safety assessment of regional traditional foods.2. The research identified Klebsiella pneumoniae ssp. pneumoniae and Staphylococcus arlettae as bacterial contaminants, highlighting potential risks from both environmental and human-related sources.3. The findings emphasize the role of packaging materials and market hygiene in bacterial contamination, offering practical recommendations for safer food handling and storage. AbstractMaluku Province in Indonesia is known for its diverse traditional foods, including sagu lempeng, which is widely consumed across different social groups. However, poor hygiene during its preparation, packaging, and sale in traditional markets can serve as a medium for microbial contamination, posing potential health risks. In Ambon City, the capital of Maluku, no research had been conducted concerning the total plate count (TPC) analysis and identification of food-contaminating bacteria in sagu lempeng, which are crucial for ensuring its microbiological safety. Therefore, this study aimed to assess the microbiological quality of sagu lempeng according to Indonesian National Standards (INS 7388:2009) and to identify any presence of food-contaminating bacteria. This research employed a quantitative descriptive methodology with a true experimental laboratory approach. The samples used were sagu lempeng collected from three traditional markets in Ambon, Indonesia. The TPC analysis was conducted to estimate the number of bacterial colonies per gram of samples, while Gram staining and biochemical testing were carried out for macroscopic and microscopic bacterial identification. The results showed that the highest TPC was found in sagu lempeng from Waiheru Market at 8.1 × 10⁵ CFU/g, while the lowest was from Mardika Market at 5.95 × 10³ CFU/g. The identified bacteria included Klebsiella pneumoniae ssp. pneumoniae and Staphylococcus arlettae. Although bacterial contamination was detected, the TPC of sagu lempeng from all sampled markets remained under the maximum allowable limit set by Indonesian National Standards (1 × 10⁶ CFU/g). In conclusion, sagu lempeng sold in traditional markets around Ambon complies with microbiological safety standards and is considered safe for consumption.
Co-Authors Abbas, Muthmainnah Ade Nurhayati Kusumadewi Adelia Mantong Alessandra Flowrence Saija Alimudi, Saiful Anggun Lestari Husein Astuti Nur Fadhilah ASTUTI, EKA Astuty, Eka Augie Desvie Klausya Kadun B. Bension, Johan Bandjar, Fitri Kadarsih Bandjar, Fitri Kardasih Cucu Hodijah de Lima, Filda V.I. de Lima, Filda Vionita Irene Dilya Maghfirah Taher DRIANA, Elin Dylan Tamalsir Dylan Tamalsir Effendi Effendi Eka Astuti Eka Astuty Elpira Asmin Elpira Asmin Elpira Asmin Erin D Noya Ernawati Ernawati Esmeraldine, Genevieva Etrin Zulqarnain Eka Saputra Linggar Evangelista Risalia Haurissa Fadila Tri Starlia Farah Christina Noya Filda de Lima Grace Latuheru Halidah Rahawarin Hartono Nurlette Hasibuan, Andrian Bayo Arif Hasna Vuspita Latuconsina Hataul, Is Asma’ul Haq Henok Siboka Onim Hursepuny, Valentine Husein, Anggun Lestary Ilsan, Noor Andryan Indas Wari Rahman Inggrain, Maulin Inggraini, Maulin Intania Riska Putrie, Intania Riska Irwan Ivanmorl Ruspanah Jennifer Vivian Kiriwenno Jepri Agung Priyanto, Jepri Agung Johan B Bension Johan Bruyif Bension Kusnara, Hasti Pramesti Latuheru, Grace Lesbatta, Kezia Josawel Mailoa, Joice Mainase, Josepina Makatita, Miftah H Marasabessy, Rendi Marasabessy, Rendy Mariyatul Qibtiyah Maxwell Landri Vers Malakauseya Metekohy, Meilani Mus, Rosdiana Musaid, Hendra Mutmainnah Abbas Myllisa Citra Wulandari Nahumarury, Salmin Nahumarury, Salmin Safril Noviyanter Siahaya Noya, Farah C Nurdin, Sukmawati Nurul Kusuma Dewi Ohiwal, Morgan Patteilohy, Merry Presly G. Siahaya Rachmawati Dwi Agustin Rahawarin, Halidah Ramanda, Viqih Ramla, Razita Ratau, Asria Reubun, Ririn Jihan S Rosalina, Febrianti Rumatiga, Masdar Ruth Magdalena Lumbantobing Safinah Yulianty Sitania Sipriyadi Siti Nur Azizah Siti Nurfajriah Sofia Mustamu Soumena, Rifah Zafarani Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati, Sukmawati Sulfiana Sulfiana SULFIANA, SULFIANA Tahitu, Ritha Talakua, Gratia Tamtelatihu, Carmila L Titin Agustina Titin Agustina Umban Adi Jaya Usman Umarella Valentine Hursepunny Victor Leo Nolly Kewilaa Vina Z. Latuconsina Warella, Juen Carla Yensy Lolita Salamor Yosephina Manse Yuliawati , Sri Yuniasih Mulyani Jubeliene Taihuttu