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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Berkala Perikanan Terubuk Biogenesis: Jurnal Ilmiah Biologi Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Ilmiah Pertanian Jurnal Penelitian Pendidikan IPA (JPPIPA) Chimica et Natura Acta Akta Kimia Indonesia JURNAL PHOTON Unri Conference Series: Community Engagement Narra J International Journal of Basic and Applied Science Dinamika Pertanian Jurnal Natur Indonesia
Titania Tjandrawati Nugroho
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health

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Aktivitas Enzim Protease dan Lipase Viscera Ikan Kembung (Restrelliger sp) pada pH Dan Konsentrasi Garam Berbeda Bustari Hasan; Desmelati Desmelati; Dian Iriani; Titania Nugroho; M Andesba Arifin; Andhra Syatapahana
Berkala Perikanan Terubuk Vol 43, No 2 (2015): Juli 2015
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.734 KB) | DOI: 10.31258/terubuk.43.2.68 - 76

Abstract

This research was conducted to measure the activity of visceral protease and lipase of mackerel (Rastrelligersp) at different pH and salt concentration. Mackerel, weighing ± 150 g/each were obtained from a fish market in Pekanbaru. Fish weretransported to the laboratory; and at the laboratory, the fish were eviscerated and their visceral organs (intestines, kidneys, liver, spleen, cord, pancreas and gonad) were blended in Tris-HCl buffer and filtered for their crude enzyme extract. The protease activity was measured at pH 6, 7, 8, 9, 10 and 11; and lipase activity was measured at pH2, 3, 4, 5, 5.5, 6, 7, 8, 9, 10 and 11. The protease and lipase activity werealso measured atsalt concentration 0%, 3%, 6%, 9%, 12% and 15%. The results showed that the protease and lipase activity varies with pH value and salt concentration. Protease activity at pH 11 (1,610µmol/ml)and salt concentration 6%(0,869 µmol/ml)showed the highest activity; and pH 10 (0,031µmol/ml) and salt concentration 15% (0,073µmol/ml) was the lowest activity. Lipase activity at pH 4 (2,428 µmol/ml) and salt concentration 3% (4,131µmol/ml) was the highest activity; and pH 11 (0,042µmol/ml) and salt concentration 15% (2,237µmol/ml) was the lowest activity. The protease and lipase activity were decreased withthe increasing of salt concentration.
EKSTRAKSI DNA DAN AMPLIFIKASI ITS rDNA ISOLAT FUNGI ENDOFIT LBKURCC67 UMBI TANAMAN DAHLIA (DAHLIA VARIABILIS) Senjavi Rakhmana; Saryono '; Titania Tjandrawati Nugroho
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

> Endophyte fungi lives in the plant tissues without causing harm to their host and has known that can produce secondary metabolite and extracellular enzyme. Fungi LBKURCC67 is an endophyte fungi that was isolated from tubers of yellow flowered Dahlia variabilis in Padang Panjang, West Sumatera. Species of fungi LBKURCC67 isolate was not known exactly because the morphology identification has been previously is appropriate for genus level. The accurate identification to known species is molecular with phylogenetic analysis. Before determine species in molecular analysis, it must extraction and amplification DNA. This research aims to optimation of DNA extraction and rDNA amplification in Internal Transcribed Spacer (ITS) regions with Polymerase Chain Reactions (PCR) methods. The DNA of fungi LBKURCC67 isolate was extract with Wizard Genomic DNA Purification kit ex Promega Corp. (Madison, USA) from cultur mycelium. The result shows extraction DNA was success from fourth days mycelia. Optimum condition for rDNA amplification with PCR were used ITS5 and ITS4 primers and 41°C annealing temperature. Electrophoresis analysis shows molecular weight of DNA isolate is 16.951 bp and molecular weight of PCR product is 583 bp.
HUBUNGAN AKTIVITAS ENZIM DAN KONSENTRASI SUBSTRAT PADA POLA DETEKSI SECARA HPLC HASIL TRANSGLIKOSILASI PINOCEMBRIN OLEH ENZIM SELULASE Trichoderma asperellum LBKURCC1 Fifira Safitri; Titania Tjandrawati Nugroho; Hilwan Yuda Teruna
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

"> Enzymatic transglycosylation of pinocembrin was successfully carried out by cellulase from Trichoderma asperellum LBKURCC1. However, the reproducibility of the reaction is low, making detection by reverse phase HPLC (High Performance Liquid Chromatography) difficult when the enzyme activity decreases. It is believed that the initial flavonoid concentration used in the transglycosylation reaction influences the detection pattern of transglycosylation product by HPLC, when the enzyme activity is low. In this research, transglycosylation of pinocembrin was done by a concentrated extract of T. asperellum LBKURCC1 cellulase and using CMC (Carboxymethyl Cellulose) as glycosyl donor. The reaction was carried out for 30 hours at 40oC and 170 rpm shaking. Results of HPLC analysis showed that cellulase with activity of (4.3±0.2) U/mL could only obtain 1.7% conversion of pinocembrin to its glycosylated product when the substrate concentration was 6 mg/mL. At these conditions, a barely detectable HPLC peak was observed at retention time of 8.4 minutes. This peak increased noticeably when the initial concentration of pinocembrin was decreased to 0.6 mg/mL. Percent conversion of pinocembrin to its transglycosylation product was 4.3% when the initial substrate concentration was 0.6 mg/mL and the enzyme activity was (4.3±0.2) U/mL.
UJI AKTIVITAS ENZIM PROTEASE DARI ISOLAT Bacillus sp. GALUR LOKAL RIAU Rani Yuniati; Titania Tjandrawati Nugroho; Fifi Puspita
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Proteolytic bacteria are bacteria that are able to hydrolize proteins into smaller peptides or completely into amino acid units. This study was carried out to determine the proteolytic activity of Bacillus sp. B1 isolated from rhizosphere of mustard plants. The proteolytic activity of the enzymes were determined based on the amount of tyrosin liberated in Unit/mL, while the specific activity of the enzymes were indicated by activity per unit weight of proteins. The protein concentration of enzyme  extracts were determined by the Lowry method. The activity of enzyme was determined at various production times (6, 12, 18, 24, 30, and 36 hours). The results showed that the Bacillus sp. B1 isolate was able to produce protease. Bacillus sp. B1 isolate obtained its maximum activity in 30 hours of production time. The specific activity of protease Bacillus sp. B1 was 0,2523 ± 0,0050 Unit/mg proteins.Proteolytic bacteria are bacteria that are able to hydrolize proteins into smaller peptides or completely into amino acid units. This study was carried out to determine the proteolytic activity of Bacillus sp. B1 isolated from rhizosphere of mustard plants. Theproteolytic activity of the enzymes were determined based on the amount of tyrosin liberated in Unit/mL, while the specific activity of the enzymes were indicated by activity per unit weight of proteins. The protein concentration of enzyme extracts were determined by the Lowry method. The activity of enzyme was determined at various production times (6, 12, 18, 24, 30, and 36 hours). The results showed that the Bacillus sp. B1 isolate was able to produce protease. Bacillus sp. B1 isolate obtained itsmaximum activity in 30 hours of production time. The specific activity of protease Bacillus sp. B1 was 0,2523 ± 0,0050 Unit/mg proteins.
ISOLASI DNA DAN AMPLIFIKASI PCR DAERAH ITS rDNA FUNGI ENDOFIT UMBI TANAMAN DAHLIA (Dahlia variabilis) LBKURCC69 Fitri Rahayu; Saryono '; Titania Tjandrawati Nugroho
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Endophytic fungi lives within healthy plant tissues without causing damage to the host plant. Endophytic fungi LBKURCC69 was one of the endophytic fungi that was isolated from the tubers of dahlia plant (Dahlia variabilis). Morphological identification showed that endophytic fungi LBKURCC69 was Phialophora fastigiata. This identification can’t provide an accurate result because many species of fungi with the same morphological features, that causing misidentification. More accurate species identification can be done by molecular identification using rDNA ITS region. Electrophoresis results indicated that chromosomal DNA of endophytic fungi LBKURCC69 was successfully isolated and has a molecular weight of 6124 bp. DNA amplification of endophytic fungi LBKURCC69 on rDNA ITS regions was successfully performed using ITS4 and ITS5 primers with 45°C annealing temperature and produces DNA fragments with a molecular weight of 537 bp.
HUBUNGAN AKTIVITAS ENZIM DAN KONSENTRASI SUBSTRAT PADA POLA DETEKSI SECARA HPLC HASIL TRANSGLIKOSILASI NARINGENIN OLEH ENZIM SELULASE Penicillium sp. LBKURCC27 Puspita Sari; Titania Tjandrawati Nugroho; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Transglycosylation of naringenin was carried out by concentrated cellulase enzyme of Penicillium sp. LBKURCC27. Enzymatic transglycosylation of naringenin was successfully carried out by cellulase from Penicillium sp. LBKURCC27, however the reproducibility of the reaction is low, making detection by reverse phase HPLC (High Performance Liquid Chromatography) difficult when the enzyme activity decreases. It is believe that the flavonoid concentration used in the transglycosylation naringenin by cellulase Penicillium sp. LBKURCC27 influences the detection pattern of transglycosylation product of HPLC. The reaction was carried out for 30 hours at 40°C with acetate buffer 0.05 M pH 5.5 and 170 rpm shaking. Carboxymethyl Cellulose (CMC) was used as glycosyl donor. Results of HPLC analysis showed that cellulase Penicillium sp. LBKURCC27 with an activity of (0.670±0.023 U/mL) were not able to convert naringenin to a detectable glycosylated product when the initial substrate concentration was 6 mg/mL. In the 0.6 mg/mL of naringenin concentration, the glycosylation product was formed with 100% of percent convertion.
TRANSGLIKOSILASI ENZIMATIK SENYAWA ANTIOKSIDAN PINOCEMBRIN MENGGUNAKAN SELULASE TRICHODERMA RESEEI UNTUK PENINGKATAN BIOAVAILABILITASNYA Nova Rianti Putri; Harni Sepriani; Hilwan Yuda Teruna; Titania Tjandrawati Nugroho
Sistem Informasi Vol 5 No 1 (2014): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v5i1.389

Abstract

Flavonoid merupakan senyawa polifenol yang paling banyak terdapat di alam. Senyawa ini umumnya ditemukan dalam bentuk tidak terikat dengan gula (flavonoid aglikon). Salah satu contohnya adalah flavonoid aglikon pinocembrin. Flavonoid aglikon pinocembrin merupakan senyawa antioksidan, dan terbukti memiliki sifat antiangiogenesis, anti-inflamasi dan anti-tumor. Senyawa ini belum digunakan dalam jumlah banyak dikarenakan kelarutannya dalam air rendah, tidak stabil terhadap pengaruh cahaya, mudah teroksidasi, dan penyerapan di dalam usus rendah, serta memiliki rasa pahit. Sifat bioavailabilitas flavonoid aglikon ini dapat ditingkatkan dengan melakukan reaksi transglikosilasi. Transglikosilasi merupakan reaksi pemindahan unit gula ke akseptor yang memiliki gugus -OH. Flavonoid aglikon pinocembrin dapat bersifat stabil dan kelarutannya dalam air meningkat apabila diubah menjadi bentuk glikosida sebagai flavonoid glikosida melalui reaksi transglikosilasi secara enzimatik. Dalam penelitian ini transglikosilasi enzimatik pinocembrin dilakukan menggunakan bantuan enzim selulase Trichoderma reseei. Reaksi transglikosilasi dilakukan selama 30 jam pada suhu 40oC, menggunakan buffer asetat 0,05M pH 5, dan kecepatan pengocokan 170 rpm. Substrat carboxymethylcellulose (CMC) digunakan sebagai donor glikosil. Flavonoid glikosida hasil reaksi transglikosilasi dianalisis menggunakan High Performance Liquid Chromatography (HPLC). Hasil analisis HPLC menunjukkan enzim T. Reseei mampu melakukan reaksi transglikosilasi terhadap pinocembrin yang dapat dilihat dari adanya perubahan nilai waktu retensi dari produk transglikosilasi dibandingkan sebelum reaksi.
PENENTUAN KADAR TANIN DALAM PELARUT ETANOL 50% DARI KULIT BUAH MANGGIS (Garcinia mangostana L.) DENGAN BANTUAN SELULASE Trichoderma asperellum LBKURCC1 - Miranti; Titania Tjandrawati Nugroho; Hilwan Yuda Teruna
Sistem Informasi Vol 6 No 02 (2016): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v6i02.434

Abstract

Tanin merupakan senyawa turunan polifenol yang memiliki berat molekul 500-3000 yang memiliki gugus hidroksi fenolik. Tanin yang terkandung dalam kulit buah manggis (Garcinia mangostana L.) dapat dimanfaatkan sebagai bahan aditif pada bahan industri cat, tekstil dan penyamak kulit. Penggunaan enzim selulase dalam proses ekstraksi tanin menggunakan alkohol 50% diharapkan mampu meningkatkan ekstrak kadar tanin. Kulit buah manggis diekstraksi dengan dan tanpa enzim selulase Trichoderma asperellum LBKURCC1 menggunakan pelarut bufer Naasetat 0,05 M pH 5,5 dan bufer-etanol dengan konsentrasi etanol 50%. Kandungan total tanin dianalisis menggunakan metoda Folin-Denis. Hasil penelitian menunjukkan kandungan tanin per gram kulit buah manggismeningkat secara signifikan (p<0,05) dengan penambahan etanol 50% dibandingkan tanpa etanol. Sedangkan kandungan tanin per gram kulit buah manggis dengan perlakuan tanpa dan menggunakan enzim selulase tidak memberikan perbedaan hasil yang nyata (p≥0,05).
IDENTIFIKASI ISOLAT FUNGI ENDOFIT LBKURCC43 BERDASAR SEKUENS ITS rDNA DARI UMBI TANAMAN DAHLIA (DAHLIA VARIABILIS) Sefni Hendris; Titania T Nugroho; - Saryono
Sistem Informasi Vol 5 No 2 (2015): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v5i2.578

Abstract

Fungi LBKURCC43 merupakan fungi endofit yang diisolasi dari umbi tanaman dahlia berbunga ungu (Dahlia variabilis) di Padang Panjang, Sumatera Barat. Identifikasi secara morfologi isolat tersebut telah dilakukan dan hanya mengidentifikasi pada tingkat genus. Identifikasi secara molekuler dengan menggunakan DNA adalah identifikasi spesies yang lebih tepat digunakan. Sebelum dilakukan analisis filogenetik secara molekuler berdasarkan sekuens DNA ribosomal pada daerah ITS-1 dan ITS-2, dilakukan ekstraksi DNA dan amplikasi PCR ITS rDNA yang baik untuk sekuensing. DNA kromosomal diisolasi menggunakan kit Wizard Genomic Purification ex Promega Co (Madison, USA) dari sel miselia berumur tiga hari. Hasil penelitian menunjukkan bahwa DNA berhasil diisolasi dari miselia yang berumur tiga hari sebelum terbentuk spora dan jumlah yang cukup tinggi untuk PCR. DNA kromosomal fungi LBKURCC43 memiliki BM (berat molekul) 10.294 pb. ITS rDNA berhasil diamplifikasi dengan PCR menggunakan pasangan primer ITS5 dan ITS4, suhu annealing untuk 440C dan menghasilkan produk PCR dengan berat molekul 455 pb. Hasil analisis filogenetik daerah ITS-1, ITS-2 dan 5,8S rDNA dari genom fungi LBKURCC43 menunjukkan bahwa spesies dari fungi LBKURCC43 adalah Hanseniaspora uvarum dengan kemiripan identitas mencapai 97%.
Penerapan teknologi biopori dalam pencegahan banjir dan kekeringan yang sekaligus pembuatan biokompos di Kelurahan Delima Kecamatan Tampan Pekanbaru Andi Dahliaty; Yuana Nurulita; Titania Tjandrawati Nugroho; Sri Helianty
Unri Conference Series: Community Engagement Vol 1 (2019): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.1.255-261

Abstract

Flooding is a problem that almost every year affects urban and rural areas. Generally, in urban areas, floods are caused more by the lack of smooth flow of water (sewage) due to waste dumped into the water stream and the reduction of water catchment areas. The Delima Subdistrict of Tampan Subdistrict has a simple housing area that has a limited home yard measuring around 7m x13m up to 10m x 15m per house unit. To meet clean water needs, this area uses bore wells which over time will reduce underground water reserves. The housing area is sometimes disemenised, which results in a lack of infiltration of rain water so that it is prone to flooding and drought. To overcome this, the community can apply biopore infiltration technology. This service activity aims to introduce biopore infiltration technology and invites the community to apply it and make biocompost from the infiltration hole. The method of service activities was carried out with material delivery techniques and discussions as well as demonstrations or practices in making biopori infiltration holes as an effort to overcome floods and droughts in settlements around the Delima Village, Tampan District, Pekanbaru. From the results of this activity it is known that the target community seemed enthusiastic about the material presented and there was an increase in the knowledge and insights of the participants about biopori absorption technology by around 80%.
Co-Authors - Miranti Adja Muhammad Alsan Shaf Andhra Syatapahana Andi Dahliaty Andi Dahliaty Andi Dahliaty Annisa Fitri Ayu Octa Mellani Bustari Hasan Citra Hardiyanti Desmelati Desmelati Dian Iriani Dian Novita Sary Edy Fachrial Feliatra Fifi Puspita Fifi Puspita Fifira Safitri Fitri Rahayu Fitri, Nur Annisa Gaol, Fuji Randa Lumban Gurning, Esther Angelita Halida Sophia Harni Sepriani Hilwan Yuda Teruna Iin Evita Sari Ismawati ismawati Ismawati Ismawati Jati, Afif P Jeky Sasemar Lumban Khairullinas Khairullinas M Andesba Arifin Miranti Miranti Miranti Miranti Miranti Miranti Miranti, - Narasswati, Nungki Nenci, Nuryani Nova Rianti Putri Novia Sellyna Nur Azlina Nuria Wulandari Nurwijayanti Nuryani Nenci Oktavia, Rani Puja, Krisna Puspita Sari Putri Nabilah Putri, Nova Rianti Ramadhan, Gilang Rani Yuniati Sakinah, Alifia Andra Saputra, Rachmad Saryono Saryono Saryono &#039; Saryono Saryono Saryono, M.Or. S.Pd. Jas. Senjavi Rakhmana Sepriani, Harni Seprianto, Seprianto Sila Silalahi Siregar, Siti Saidah Sri Helianty suraya, nabella Susilawati, Susilawati Utama, Panca Setia Wahyuningsih Wahyuningsih Yanti Lim Yanti Yanti Yanti Yanti Yuana Nuralita Yuana Nurulita Yuana Nurulita Yuharmen - Yuharmen Yuharmen Yum Eryanti Yuslina Octavia Zona Octarya Zona Octarya