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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Berkala Perikanan Terubuk Biogenesis: Jurnal Ilmiah Biologi Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Ilmiah Pertanian Chimica et Natura Acta Akta Kimia Indonesia JURNAL PHOTON Unri Conference Series: Community Engagement Narra J International Journal of Basic and Applied Science PENA ABDIMAS : Jurnal Pengabdian Masyarakat Dinamika Pertanian Jurnal Natur Indonesia
Titania Tjandrawati Nugroho
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Bioremediation, Biosorption, and Biodegradation of the Textile Dye Reactive Black 5 by Life Cultures of Trichoderma asperellum LBKURCC1 Titania Tjandrawati Nugroho; Putri Nabilah; Nuria Wulandari; Yuana Nuralita; Andi Dahliaty; Yanti Yanti
Jurnal Natur Indonesia Vol. 21 No. 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Reactive black 5 (RB5) is an azo dye widely used in the textile industry for dyeing fabrics. It is categorized as a recalcitrant dye that is hard to degrade and an environmental pollutant. Therefore, textile waste effluents containing this dye must be treated to remove or degrade the dye, before being released into the environment. One method that can be used to degrade synthetic dyes such as RB5 is to use biological methods, by directly using live fungal cells or using laccase enzymes. Trichoderma asperellum LBKURCC1 is a filamentous fungus isolated from cacao plantation soil in Riau, Indonesia, and it is a laccase enzyme producer. To be able to determine the ability of T.asperellum LBKURCC1 life cultures to decolorize RB5 dye, several RB5 dye removal tests were carried out. Incubation of 50 ppm RB5 with life cultures of T. asperellum LBKURCC1 at room temperature (30°C, pH 6.5) for 24 hours resulted in 22% bioremediation, 3.2% biosorption and 19.1% biodegradation of the RB5 dye. The results of this study show that the live culture of T.asperellum LBKURCC1 is capable of biodegrading RB5. This is indicated by the degradation of RB5 by extracellular enzymes produced by these filamentous fungi.
Bioremediation, Biosorption, and Biodegradation of the Textile Dye Reactive Black 5 by Life Cultures of Trichoderma asperellum LBKURCC1 Titania Tjandrawati Nugroho; Putri Nabilah; Nuria Wulandari; Yuana Nuralita; Andi Dahliaty; Yanti Yanti
Jurnal Natur Indonesia Vol. 21 No. 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.2.93-99

Abstract

Reactive black 5 (RB5) is an azo dye widely used in the textile industry for dyeing fabrics. It is categorized as a recalcitrant dye that is hard to degrade and an environmental pollutant. Therefore, textile waste effluents containing this dye must be treated to remove or degrade the dye, before being released into the environment. One method that can be used to degrade synthetic dyes such as RB5 is to use biological methods, by directly using live fungal cells or using laccase enzymes. Trichoderma asperellum LBKURCC1 is a filamentous fungus isolated from cacao plantation soil in Riau, Indonesia, and it is a laccase enzyme producer. To be able to determine the ability of T.asperellum LBKURCC1 life cultures to decolorize RB5 dye, several RB5 dye removal tests were carried out. Incubation of 50 ppm RB5 with life cultures of T. asperellum LBKURCC1 at room temperature (30°C, pH 6.5) for 24 hours resulted in 22% bioremediation, 3.2% biosorption and 19.1% biodegradation of the RB5 dye. The results of this study show that the live culture of T.asperellum LBKURCC1 is capable of biodegrading RB5. This is indicated by the degradation of RB5 by extracellular enzymes produced by these filamentous fungi.
Metabolomic profiling and antimicrobial investigation of Aspergillus fumigatus LBKURCC269 and Bacillus paramycoides LBKURCC218 co-culture Octarya, Zona; Nugroho, Titania T.; Nurulita, Yuana; Suraya, Nabella; Saryono, Saryono
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.1647

Abstract

The increasing resistance of pathogenic microbes to antibiotics is a major public health concern, necessitating the discovery of effective antimicrobial compounds. The aim of this study was to assess the bioactive metabolites produced by Aspergillus fumigatus LBKURCC269 and Bacillus paramycoides LBKURCC218 under three fermentation conditions: monoculture of each microorganism and their co-culture. Metabolite analyses initiated with gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) followed with molecular networking–Global Natural Products Social Molecular Networking (GNPS) and molecular docking. Antimicrobial activity of the extracts was then conducted. Metabolite analysis using GC-MS identified key antimicrobial compounds, including 2,6-bis(1,1-dimethylethyl)-4-methylphenol, pentadecanoic acid, cyclopropane pentanoic acid, and 3-piperidinol. LC-HRMS, combined with multivariate analysis and GNPS molecular networking, revealed additional antimicrobial compounds, including novel pyrazine derivatives induced in co-culture fermentation. Molecular docking analysis of 3-(propan-2-yl)-octahydropyrrolo[1,2-a]pyrazine-1,4-dione demonstrated its potential as an antimicrobial agent by inhibiting topoisomerase IV and cytochrome monooxygenase with binding affinity of -5.34 kcal/mol and -5.6 kcal/mol, respectively. The antimicrobial assays showed that the co-culture fermentation extract had the strongest activity, with inhibition zones of 20.33±0.59 mm (Escherichia coli), 14.33±0.59 mm (Staphylococcus aureus), and 25.67±0.59 mm (Candida albicans). This study highlights the advantages of co-culture fermentation in enhancing the discovery of antimicrobial compounds. The findings underscore the potential of this approach to simplify chemical isolation and accelerate the identification of novel antimicrobial agents for pharmaceutical development.
α-glucosidase Inhibitory Activity of Probiotic Isolate LBSU9 Isolated from Traditional Food “Trites”: a Preliminary Study Fachrial, Edy; Ismawati, Ismawati; Nugroho, Titania T; Saryono, Saryono
International Journal of Basic and Applied Science Vol. 12 No. 4 (2024): March: Basic and Applied Science
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/ijobas.v12i4.293

Abstract

As the prevalence of type 2 diabetes mellitus continues to increase over the years, research into antidiabetic drugs needs to continue. Probiotics are non-pathogenic microorganisms that have potential as α-glucosidase inhibitors. This study aimed to characterize and determine the α-glucosidase inhibitor activity of LBSU9 isolate, a probiotic isolated from "trites," a traditional food of North Sumatra. The results showed that LBSU9 had bacillus morphology, Gram positive, negative catalase test, TSIA (Triple Sugar Iron Agar) A/A test and non-hemolysis. LBSU9 had good tolerance to simulated gastric acid and bile salts, with growth percentages of 66.54% and 64.74%. LBSU9 also has potent antimicrobial activity against S.aureus and E.coli, with 20 mm and 17 mm inhibition zones, respectively. The alpha-glucosidase inhibitor activity of LBSU9 was 98.4% greater than that of acarbose, which was 97%. Based on the results of this study, it is concluded that LBSU9 isolate has the potential to be a complementary therapy to prevent or treat type 2 diabetes mellitus
Penerapan Teknik Minibiopori Untuk Tanaman Hortikultura dan Analisis Dampak Ekonomi Masyarakat Dahliaty, Andi; Sophia, Halida; Azlina, Nur; Siregar, Siti Saidah; -, Susilawati; Nugroho, Titania Tjandrawati; Safitri, Selfi Yanti
PENA ABDIMAS : Jurnal Pengabdian Masyarakat Vol 7 No 1 (2026): Januari 2026
Publisher : LPPM Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pengelolaan sampah organik rumah tangga di Desa Sungai Berbari, Kecamatan Pusako, Kabupaten Siak, merupakan permasalahan yang mempengaruhi kondisi ekonomi dan lingkungan. Kegiatan pengabdian Masyarakat ini bertujuan untuk menerapkan teknik minibiopori dalam pengelolaan sampah organik sekaligus meningkatkan produktivitas lahan pekarangan untuk tanaman hoktikultura dan analisis dampak ekonomi terhadap penerapan teknik minibiopori. Metode yang digunakan meliputi sosialisasi, pelatihan pembuatan biopori, dan pendampingan implementasi. Evaluasi kegiatan menunjukkan hasil yang sangat signifikan, diantaranya peningkatan pengetahuan peserta tentang biokompos hingga 90%, pengurangan biaya pupuk sebesar 80%, dan peningkatan pendapatan rumah tangga hingga 75%. Selain itu, program ini dinilai mudah diterapkan dan berpotensi membuka peluang usaha baru serta mendukung ketahanan pangan desa. Hasil ini menunjukkan bahwa teknik minibiopori memberikan manfaat ekonomi dan keberlanjutan lingkungan bagi masyarakat desa.
Co-Authors - Miranti Adja Muhammad Alsan Shaf Andhra Syatapahana Andi Dahliaty Andi Dahliaty Andi Dahliaty Annisa Fitri Ayu Octa Mellani Bustari Hasan Citra Hardiyanti Desmelati Desmelati Dian Iriani Dian Novita Sary Edy Fachrial Feliatra Fifi Puspita Fifi Puspita Fifira Safitri Fitri Rahayu Fitri, Nur Annisa Gaol, Fuji Randa Lumban Gurning, Esther Angelita Halida Sophia Harni Sepriani Hilwan Yuda Teruna Iin Evita Sari Ismawati Ismawati Jeky Sasemar Lumban Khairullinas Khairullinas M Andesba Arifin Miranti Miranti Miranti Miranti Miranti Miranti Miranti, - Narasswati, Nungki Nenci, Nuryani Nova Rianti Putri Novia Sellyna Nur Azlina Nuria Wulandari Nurwijayanti Nuryani Nenci Oktavia, Rani Puja, Krisna Puspita Sari Putri Nabilah Putri, Nova Rianti Ramadhan, Gilang Rani Yuniati Safitri, Selfi Yanti Sakinah, Alifia Andra Saputra, Rachmad Saryono Saryono ' Saryono Saryono Saryono, M.Or. S.Pd. Jas. Senjavi Rakhmana Sepriani, Harni Seprianto, Seprianto Sila Silalahi Siregar, Siti Saidah Siregar, Siti Saidah Sri Helianty suraya, nabella Susilawati - Susilawati, Susilawati Utama, Panca Setia Wahyuningsih Wahyuningsih Yanti Lim Yanti Yanti Yanti Yanti Yuana Nuralita Yuana Nurulita Yuana Nurulita Yuharmen - Yuharmen Yuharmen Yum Eryanti Yuslina Octavia Zona Octarya Zona Octarya