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Bioremediation, Biosorption, and Biodegradation of the Textile Dye Reactive Black 5 by Life Cultures of Trichoderma asperellum LBKURCC1 Titania Tjandrawati Nugroho; Putri Nabilah; Nuria Wulandari; Yuana Nuralita; Andi Dahliaty; Yanti Yanti
Jurnal Natur Indonesia Vol. 21 No. 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Reactive black 5 (RB5) is an azo dye widely used in the textile industry for dyeing fabrics. It is categorized as a recalcitrant dye that is hard to degrade and an environmental pollutant. Therefore, textile waste effluents containing this dye must be treated to remove or degrade the dye, before being released into the environment. One method that can be used to degrade synthetic dyes such as RB5 is to use biological methods, by directly using live fungal cells or using laccase enzymes. Trichoderma asperellum LBKURCC1 is a filamentous fungus isolated from cacao plantation soil in Riau, Indonesia, and it is a laccase enzyme producer. To be able to determine the ability of T.asperellum LBKURCC1 life cultures to decolorize RB5 dye, several RB5 dye removal tests were carried out. Incubation of 50 ppm RB5 with life cultures of T. asperellum LBKURCC1 at room temperature (30°C, pH 6.5) for 24 hours resulted in 22% bioremediation, 3.2% biosorption and 19.1% biodegradation of the RB5 dye. The results of this study show that the live culture of T.asperellum LBKURCC1 is capable of biodegrading RB5. This is indicated by the degradation of RB5 by extracellular enzymes produced by these filamentous fungi.

Bioremediation, Biosorption, and Biodegradation of the Textile Dye Reactive Black 5 by Life Cultures of Trichoderma asperellum LBKURCC1 Titania Tjandrawati Nugroho; Putri Nabilah; Nuria Wulandari; Yuana Nuralita; Andi Dahliaty; Yanti Yanti
Jurnal Natur Indonesia Vol. 21 No. 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.2.93-99

Reactive black 5 (RB5) is an azo dye widely used in the textile industry for dyeing fabrics. It is categorized as a recalcitrant dye that is hard to degrade and an environmental pollutant. Therefore, textile waste effluents containing this dye must be treated to remove or degrade the dye, before being released into the environment. One method that can be used to degrade synthetic dyes such as RB5 is to use biological methods, by directly using live fungal cells or using laccase enzymes. Trichoderma asperellum LBKURCC1 is a filamentous fungus isolated from cacao plantation soil in Riau, Indonesia, and it is a laccase enzyme producer. To be able to determine the ability of T.asperellum LBKURCC1 life cultures to decolorize RB5 dye, several RB5 dye removal tests were carried out. Incubation of 50 ppm RB5 with life cultures of T. asperellum LBKURCC1 at room temperature (30°C, pH 6.5) for 24 hours resulted in 22% bioremediation, 3.2% biosorption and 19.1% biodegradation of the RB5 dye. The results of this study show that the live culture of T.asperellum LBKURCC1 is capable of biodegrading RB5. This is indicated by the degradation of RB5 by extracellular enzymes produced by these filamentous fungi.

Metabolomic profiling and antimicrobial investigation of Aspergillus fumigatus LBKURCC269 and Bacillus paramycoides LBKURCC218 co-culture Octarya, Zona; Nugroho, Titania T.; Nurulita, Yuana; Suraya, Nabella; Saryono, Saryono
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.1647

The increasing resistance of pathogenic microbes to antibiotics is a major public health concern, necessitating the discovery of effective antimicrobial compounds. The aim of this study was to assess the bioactive metabolites produced by Aspergillus fumigatus LBKURCC269 and Bacillus paramycoides LBKURCC218 under three fermentation conditions: monoculture of each microorganism and their co-culture. Metabolite analyses initiated with gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) followed with molecular networking–Global Natural Products Social Molecular Networking (GNPS) and molecular docking. Antimicrobial activity of the extracts was then conducted. Metabolite analysis using GC-MS identified key antimicrobial compounds, including 2,6-bis(1,1-dimethylethyl)-4-methylphenol, pentadecanoic acid, cyclopropane pentanoic acid, and 3-piperidinol. LC-HRMS, combined with multivariate analysis and GNPS molecular networking, revealed additional antimicrobial compounds, including novel pyrazine derivatives induced in co-culture fermentation. Molecular docking analysis of 3-(propan-2-yl)-octahydropyrrolo[1,2-a]pyrazine-1,4-dione demonstrated its potential as an antimicrobial agent by inhibiting topoisomerase IV and cytochrome monooxygenase with binding affinity of -5.34 kcal/mol and -5.6 kcal/mol, respectively. The antimicrobial assays showed that the co-culture fermentation extract had the strongest activity, with inhibition zones of 20.33±0.59 mm (Escherichia coli), 14.33±0.59 mm (Staphylococcus aureus), and 25.67±0.59 mm (Candida albicans). This study highlights the advantages of co-culture fermentation in enhancing the discovery of antimicrobial compounds. The findings underscore the potential of this approach to simplify chemical isolation and accelerate the identification of novel antimicrobial agents for pharmaceutical development.

α-glucosidase Inhibitory Activity of Probiotic Isolate LBSU9 Isolated from Traditional Food “Trites”: a Preliminary Study Fachrial, Edy; Ismawati, Ismawati; Nugroho, Titania T; Saryono, Saryono
International Journal of Basic and Applied Science Vol. 12 No. 4 (2024): March: Basic and Applied Science
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/ijobas.v12i4.293

As the prevalence of type 2 diabetes mellitus continues to increase over the years, research into antidiabetic drugs needs to continue. Probiotics are non-pathogenic microorganisms that have potential as α-glucosidase inhibitors. This study aimed to characterize and determine the α-glucosidase inhibitor activity of LBSU9 isolate, a probiotic isolated from "trites," a traditional food of North Sumatra. The results showed that LBSU9 had bacillus morphology, Gram positive, negative catalase test, TSIA (Triple Sugar Iron Agar) A/A test and non-hemolysis. LBSU9 had good tolerance to simulated gastric acid and bile salts, with growth percentages of 66.54% and 64.74%. LBSU9 also has potent antimicrobial activity against S.aureus and E.coli, with 20 mm and 17 mm inhibition zones, respectively. The alpha-glucosidase inhibitor activity of LBSU9 was 98.4% greater than that of acarbose, which was 97%. Based on the results of this study, it is concluded that LBSU9 isolate has the potential to be a complementary therapy to prevent or treat type 2 diabetes mellitus

Penerapan Teknik Minibiopori Untuk Tanaman Hortikultura dan Analisis Dampak Ekonomi Masyarakat Dahliaty, Andi; Sophia, Halida; Azlina, Nur; Siregar, Siti Saidah; -, Susilawati; Nugroho, Titania Tjandrawati; Safitri, Selfi Yanti
PENA ABDIMAS : Jurnal Pengabdian Masyarakat Vol 7 No 1 (2026): Januari 2026
Publisher : LPPM Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Pengelolaan sampah organik rumah tangga di Desa Sungai Berbari, Kecamatan Pusako, Kabupaten Siak, merupakan permasalahan yang mempengaruhi kondisi ekonomi dan lingkungan. Kegiatan pengabdian Masyarakat ini bertujuan untuk menerapkan teknik minibiopori dalam pengelolaan sampah organik sekaligus meningkatkan produktivitas lahan pekarangan untuk tanaman hoktikultura dan analisis dampak ekonomi terhadap penerapan teknik minibiopori. Metode yang digunakan meliputi sosialisasi, pelatihan pembuatan biopori, dan pendampingan implementasi. Evaluasi kegiatan menunjukkan hasil yang sangat signifikan, diantaranya peningkatan pengetahuan peserta tentang biokompos hingga 90%, pengurangan biaya pupuk sebesar 80%, dan peningkatan pendapatan rumah tangga hingga 75%. Selain itu, program ini dinilai mudah diterapkan dan berpotensi membuka peluang usaha baru serta mendukung ketahanan pangan desa. Hasil ini menunjukkan bahwa teknik minibiopori memberikan manfaat ekonomi dan keberlanjutan lingkungan bagi masyarakat desa.

Bioactive peptides from traditional homemade fermented foods as an alternative for treatment Nabella Suraya; Titania Tjandrawati Nugroho; Ismawati Ismawati; Saryono Saryono
Acta Chimica Asiana Vol. 8 No. 2 (2025)
Publisher : The Indonesian Chemical Society, Chapter Nusa Tenggara and The University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/aca.v8i2.252

Bioactive peptides in fermented foods are attracting scientific interest due to their prospective applications as nutraceutical and functional foods. Conventional fermented foods are naturally processed at home through the action of lactic acid bacteria (LAB). These bacteria break down food components and generate lactic acid, various organic acids, hydrogen peroxide, carbon dioxide, and short-chain peptides, which offer numerous health benefits. The bioactive peptides formed during fermentation exhibit diverse biological functions influenced by their structural compositions. Following enzymatic hydrolysis, peptides initially inactive within the native protein matrix are released and exhibit biological activity. This review aims to comprehensively examine the functional properties of bioactive peptides derived from the fermentation of traditional foods, with a particular emphasis on their therapeutic potential, including antidiabetic, antihypertensive, and anticarcinogenic activities. This review examines the health benefits of various bioactive peptides produced by lactic acid bacteria during the fermentation of traditional foods. Microbial bioactivity during the fermentation process can produce hydrolytic enzymes that break down macromolecules in food into smaller compounds, such as bioactive peptides. A wide range of peptides have been identified as improving insulin uptake, decreasing blood glucose concentrations, and inhibiting key enzymes involved in the pathogenesis of diabetes. Specific peptides exert antihypertensive effects by acting as angiotensin-converting enzyme (ACE) inhibitors, thereby preventing the transformation of angiotensin I into angiotensin II. Moreover, some bioactive peptides demonstrate immunomodulatory properties by enhancing immune responses and inducing apoptosis in cancer cells. This article provides a comprehensive overview of the therapeutic and health-promoting potentials of bioactive peptides derived from fermented foods.

HYDROLYTIC ENZYME SYSTEMS OF Trichoderma spp.: FERMENTATION STRATEGIES, STRAIN ENGINEERING, AND SUSTAINABLE LIGNOCELLULOSIC VALORIZATION Sri Helianty; Yuana Nurulita; Panca Setia Utama; Titania Tjandrawati Nugroho
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2026.15450

Hydrolytic enzymes produced by Trichoderma spp. play a central role in lignocellulosic biomass conversion due to their high secretion capacity and catalytic versatility. This review provides a critical and integrated analyzes of enzyme systems, fermentation strategies, and bioprocess engineering approaches governing enzyme production and biomass valorization. A structured literature review was conducted using peer-reviewed publication 2019 to 2025. The analysis highlights synergistic interactions among cellulases, hemicellulases, and oxidative enzymes in overcoming biomass recalcitrance. Key process parameters—including aeration, moisture content, temperature regulation, and bioreactor design—are identified as a primary deteminants of productivity and efficiency. Solid-state fermentation is recognized as an efficient strategy for valorizing agricultural residues such as rice straw, although challenges in heat and mass transfer and scale-up remain. Advances in strain engineering, regulatory pathway optimization, and enzyme cocktail design improve hydrolytic performance, but trade-offs between productivity, stability, and process robustness persist. Integration within circular biorefinery frameworks is essential for scalable and sustainable biomass conversion.

HYDROLYTIC ENZYME SYSTEMS OF Trichoderma spp.: FERMENTATION STRATEGIES, STRAIN ENGINEERING, AND SUSTAINABLE LIGNOCELLULOSIC VALORIZATION Sri Helianty; Yuana Nurulita; Panca Setia Utama; Titania Tjandrawati Nugroho
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2026.15450

Hydrolytic enzymes produced by Trichoderma spp. play a central role in lignocellulosic biomass conversion due to their high secretion capacity and catalytic versatility. This review provides a critical and integrated analyzes of enzyme systems, fermentation strategies, and bioprocess engineering approaches governing enzyme production and biomass valorization. A structured literature review was conducted using peer-reviewed publication 2019 to 2025. The analysis highlights synergistic interactions among cellulases, hemicellulases, and oxidative enzymes in overcoming biomass recalcitrance. Key process parameters—including aeration, moisture content, temperature regulation, and bioreactor design—are identified as a primary deteminants of productivity and efficiency. Solid-state fermentation is recognized as an efficient strategy for valorizing agricultural residues such as rice straw, although challenges in heat and mass transfer and scale-up remain. Advances in strain engineering, regulatory pathway optimization, and enzyme cocktail design improve hydrolytic performance, but trade-offs between productivity, stability, and process robustness persist. Integration within circular biorefinery frameworks is essential for scalable and sustainable biomass conversion.

Profil Sitotoksik ekstrak Lithocarpus bancanus dan Fraksinya Terhadap Lini Sel Kanker Paru-Paru A549 Hilwan Yuda Teruna; Rudi Hendra; Retno Puji Lestari; Titania Tjandrawati Nugroho; Saryono Saryono; Jasril Jasril; Muhammad Almurdani; Rizky Abdulah
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 18 No 1 (2026): Jurnal Farmasi Indonesia
Publisher : Pengurus Pusat Ikatan Apoteker Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfionline.v18i1.128

Lithocarpus bancanus is a medicinal plant used by communities in Riau Province, Indonesia. This plant is a tree locally known in the province as mempening. In this study, the cytotoxic activity profiles of the methanolic extract, n-hexane fraction, dichloromethane fraction, and ethyl acetate fraction from L. bancanus leaves were evaluated against human lung cancer cells (A549 cell line) using the MTT assay. IC₅₀ values were calculated from dose–response curves analyzed using the 4-parameter logistic (4PL) model. The results showed IC₅₀ values of 455.1, 361.9, 132.9, and 304.7 µg/mL for the methanolic extract, n-hexane fraction, dichloromethane fraction, and ethyl acetate fraction, respectively, with the dichloromethane fraction exhibiting relatively higher cytotoxic activity (IC₅₀ = 132.9 µg/mL). Cisplatin was used as the positive control and showed an IC₅₀ of 103.9 µg/mL. Meanwhile, the methanolic extract as well as the n-hexane and ethyl acetate fractions showed low cytotoxic activity, with IC₅₀ values greater than 300 µg/mL. Therefore, the dichloromethane fraction can serve as a basis for further isolation to identify bioactive anticancer compounds.

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