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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Berkala Perikanan Terubuk Biogenesis: Jurnal Ilmiah Biologi Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Ilmiah Pertanian Jurnal Penelitian Pendidikan IPA (JPPIPA) Chimica et Natura Acta Akta Kimia Indonesia JURNAL PHOTON Unri Conference Series: Community Engagement Narra J International Journal of Basic and Applied Science Dinamika Pertanian Jurnal Natur Indonesia
Titania Tjandrawati Nugroho
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health

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Inovasi produk olahan Singkong menjadi Singkong Frozen untuk meningkatkan pendapatan masyarakat Halida Sophia; Andi Dahliaty; Titania Tjandrawati Nugroho; Sri Helianty
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.488-493

Abstract

The cassava's production arm was quite abundant. Human ratings generally revolved around plantations and in rungs industries, much of which was said in cassava commodities. Most people consume cassava in the simple way of being boiled, fried, made in chips, and so on. In order not to dull the flavor of traditional foods but to be increasingly ogled by the styrofoampurvets, it is possible to make the products of the Singkong processed innovation into cassava frozen. The public's devoted activity is to helping people improve the economy, providing added insight and skill to processing cassava through cassava frozen and helping people form a group of tapioca women (KWT). The method used in this dedicated activity is by counseling between socializing and training and by demonstrations of Singkong frozen production. This activity involves women of family welfare development and housewives in three neighborhood associations is number 01, 02, and 03 at citizens association 12, Mentangor village districts,Tenayan Raya city districts. The result of this activity is that the target public seems enthusiastic about the material presented and has increased participants' knowledge and insight on assava frocenas are 100%.
Teknologi Biokompos dalam Biopori vertikal di Kelurahan Pematang Pudu Kecamatan Mandau Kabupaten Bengkalis Andi Dahliaty; Yuana Nurulita; Titania Tjandrawati Nugroho
Unri Conference Series: Community Engagement Vol 3 (2021): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.3.156-162

Abstract

Pematang Pudu Village is one of the villages in Mandau District, Bengkalis Regency which is approximately 116 km from Pekanbaru city. Based on information from residents of RT 02 RW 10 Km 5 Jalan Rangau, Pematang Pudu Village often floods. Floods in this area can be caused by overflowing river water, reduced rainwater catchment areas due to construction and land clearing. This biopore infiltration hole is a simple technology that does not require expensive and effective rainwater absorption. In addition, through this technology, it can reduce organic waste and make biocompost to be used as organic fertilizer that can fertilize plants. The purpose of this service activity is to provide knowledge and skills to the community about the benefits of vertical biopore technology and increase public awareness to prevent flooding. The method used in this service activity is the delivery of material using powerpoint media and field practice and involving community members in RT 02 RW 10 Pematang Pudu Village. The results of this service activity show that 38% of socialization participants have a score range between 75-80, 13.66% of socialization participants have a range of values between 85-90, and 28% of socialization participants have a range of values between 95-100. It can be stated that the provision of training has an effect on increasing public knowledge about biopore infiltration holes.
Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat. Fifi Puspita; Titania T. Nugroho; Rachmad Saputra
Jurnal Ilmiah Pertanian Vol. 19 No. 2 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i2.9402

Abstract

This research aimed to determine the potential of endophytic Trichoderma sp originated from oil palm plant tissue as a biological control agent and its molecular identification. The potency assay was carried out using a completely randomized design consisting of three isolates of Trichoderma sp. from different plant tissues (TR01 from root tissue, TS01 from stem tissue, and TM01 from midrib tissue) with inhibitory ability parameters. The inhibition of the growth of G. boninense was performed at the concentration of 15% and 20%, and the molecular identification of Trichoderma sp. endophyte isolates was analyzed using BLAST software. The results showed that Trichoderma sp. TM01 isolate had larger inhibitory than the other isolates on days 3 and 4 by 60% and 68%. TM01 isolate also showed inhibition to the growth of the G. boninense at 15% and 20%, with the percentage of inhibition reaching 71.79% and 82.05%. Based on phylogenetic analysis, the three endophytic isolates of Trichoderma sp. were closely related to Trichoderma virens.
Isolation of a High Antioxidant Non-Toxic Polar Fraction from Garcinia mangostana Fruit Pericarp by Reverse Phase Column Chromatography Wahyuningsih Wahyuningsih; Miranti Miranti; Hilwan Yuda Teruna; Titania Tjandrawati Nugroho
Jurnal Kimia Sains dan Aplikasi Vol 24, No 1 (2021): Volume 24 Issue 1 Year 2021
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3020.51 KB) | DOI: 10.14710/jksa.24.1.15-21

Abstract

The crude polar extract of mangosteen fruit pericarp not only has a moderate antioxidant activity of (55±4 μg/mL) but also has high cytotoxicity (16±0.5 μg/mL). The high cytotoxicity presumably is caused by the presence of complex cytotoxic compounds from the mangosteen pericarp. To obtain a non-toxic extract preparation with high antioxidant activity, polar crude 50% ethanol extracts of mangosteen pericarp were partially purified using reverse-phase column chromatography with Silica C18 as the stationary phase and acetonitrile-water gradient elution. Six of the ten fractions collected had high antioxidant activities, with IC50 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging antioxidant levels <50 μg/mL. Three fractions (fractions 3, 5, and 7) with the highest antioxidant activities of (16.4 ± 0.6 µg/mL), (17.8 ± 2 µg/mL) and (17.4 ± 1.8 µg/mL) respectively, were chosen for further cytotoxicity, phenolic content and High-Performance Liquid Chromatography (HPLC) analysis. The cytotoxic tests were conducted with the Brine Shrimp Lethality Assay. Fraction 3 had low cytotoxicity (LC50 485 ± 96 µg/mL) and fraction 5 was non-toxic (LC50 ≥ 1000 µg/mL), while fraction 7 still had high cytotoxicity (LC50 2.8 ± 0.8 µg/mL). The chromatogram profiles of HPLC showed that fractions 3 and 5 contained more polar compounds than the compounds present in fraction 7. It can be concluded that the reverse phase method succeeded in the isolation of a non-toxic polar fraction, that is, fraction 5, with a significantly higher (p<0.05) antioxidant activity than in the original crude polar extracts. This fraction had a high total phenolic content of 43.3 ± 0.3 g GAE per 100 g extract.
Identifikasi Metabolit Sekunder Sekresi Jamur Lokal Tanah Gambut Riau Penicillium sp. LBKURCC34 Sebagai Antimikroba Yuana Nurulita; Yuharmen Yuharmen; Annisa Fitri; Iin Evita Sari; Dian Novita Sary; Titania Tjandrawati Nugroho
Chimica et Natura Acta Vol 10, No 3 (2022)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v10.n3.45994

Abstract

Penicillium sp. LBKURCC34 adalah jamur yang diisolasi dari tanah gambut hutan primer di Giam Siak Kecil Bukit Batu (GSKBB), Cagar Biosfer di Provinsi Riau. Tujuan dari penelitian ini adalah untuk mengeksplorasi potensinya sebagai penghasil antimikroba. Metode dilakukan melalui produksi metabolit sekunder dalam media cair, ekstraksi dalam larutan etil asetat, dan pemisahan ekstrak kasar dengan Kromatografi Kolom (CC). Ekstrak kasar dan fraksi diidentifikasi dengan KLT dan HPLC dan diuji aktivitas antimikrobanya dengan metode difusi cakram. Untuk mengidentifikasi senyawa aktif dilakukan uji fitokimia dan metode KLT-Bioautografi. Produksi tiga liter media menghasilkan 0,4 gram (0,0133% b/v) ekstrak etil asetat yang diidentifikasi sebagai terpenoid dan fenolat; dan mengandung senyawa semipolar berdasarkan uji KLT dan HPLC. Hasil KLT setelah disemprot dengan p-anisaldehida 0,5% menunjukkan adanya senyawa peptaibol (peptida). Ekstrak etil asetat dapat menghambat pertumbuhan kelima mikroba patogen yang diuji namun hanya 11-45% dibandingkan dengan kontrol positif. Uji bioautografi terhadap Escherichia coli menunjukkan noda aktif zona bening pada Rf sebesar 0,67. Sayangnya, dari Fraksi 1-5 yang terdapat noda dengan Rf 0,67 masih belum menunjukkan aktivitas berarti. Penyelidikan lebih lanjut diperlukan untuk mengidentifikasi struktur senyawa aktif dari isolat lokal ini.
Microbial Growth as Determinant of Antibiotic Production with Biotic Elicitors Stimulation Citra Hardiyanti; Khairullinas Khairullinas; Jeky Sasemar Lumban; Titania Tjandrawati Nugroho; Yuana Nurulita
Jurnal Kimia Sains dan Aplikasi Vol 23, No 3 (2020): Volume 23 Issue 3 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2803.553 KB) | DOI: 10.14710/jksa.23.3.89-95

Abstract

An antibiotic-resistant and multidrug-resistant (MDR) issue open the role of researchers to continue to search for natural potential as a source of new antimicrobials. One of the potential fungi isolates that can produce antimicrobial active compounds from Indonesian tropical peat soils is Penicillium sp. LBKURCC34. In this study, the production of antimicrobial compounds from local isolates was carried out by batch fermentation method in liquid media with the addition of biotic elicitors to increase the extraction activity and yield. This study aims to optimize the results based on the time the elicitor is added. Staphylococcus aureus was used as a biotic elicitor, which was added on days 2, 3, and 4 in the production of antibiotics by fermentation incubation of 6-14 days. The antibiotic production media was extracted with ethyl acetate and evaporated. The antimicrobial test was carried out by the disk diffusion method against pathogenic bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Staphylococcus epidermidis using three crude extract contents (19; 38; and 57 µg/disc). Amoxicillin® was used as a positive control (10 µL/disc). The results showed that the addition of S. aureus biotic elicitor extended the log phase growth of the fungus Penicillium sp. LBKURCC34. The optimum condition of production was obtained by adding initiator treatment on the 3rd day for 14 days incubation with the highest yield and could inhibit the growth of all pathogenic microbes.
Bioremediation, Biosorption and Biodegradation of the Textile Dye Reactive Black 5 by Life Cultures of Trichoderma asperellum LBKURCC1 Titania Tjandrawati Nugroho; Putri Nabilah; Nuria Wulandari; Yuana Nurulita; Andi Dahliaty; Yanti Yanti
Jurnal Natur Indonesia Vol 21, No 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.2.93-99

Abstract

Reactive black 5 (RB5) is an azo dye widely used in the textile industry for dyeing fabrics. It is categorized as a recalcitrant dye that is hard to degrade and an environmental pollutant. Therefore, textile waste effluents containing this dye must be treated to remove or degrade the dye, before being released into the environment. One method that can be used to degrade synthetic dyes such as RB5 is to use biological methods, by directly using live fungal cells or using laccase enzymes. Trichoderma asperellum LBKURCC1 is a filamentous fungus isolated from cacao plantation soil in Riau, Indonesia, and it is a laccase enzyme producer. To be able to determine the ability of T. asperellum LBKURCC1 life cultures to decolorize RB5 dye, several RB5 dye removal tests were carried out. Incubation of 50 ppm RB5 with life cultures of T. asperellum LBKURCC1 at room temperature (30°C, pH 6.5) for 24 hours resulted in 22% bioremediation, 3.2% biosorption and 19.1% biodegradation of the RB5 dye.  The results of this study show that the live culture of T.asperellum LBKURCC1 is capable of biodegrading RB5. This is indicated by the degradation of RB5 by extracellular enzymes produced by these filamentous fungi.
UJI FORMULASI BIOFUNGISIDA GRANULAR BERBAHAN AKTIF Trichoderma virens ENDOFIT DALAM MENGENDALIKAN Ganoderma Boninense Pat. PADA BIBIT KELAPA SAWIT (Elaeis guineensis Jacq.) Adja Muhammad Alsan Shaf; Titania Tjandrawati Nugroho; Fifi Puspita
DINAMIKA PERTANIAN Vol. 37 No. 2 (2021): Jurnal Dinamika Pertanian Edisi Agustus 2021
Publisher : UIR Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25299/dp.2021.vol37(2).11854

Abstract

The aim of this work is to find out the effect of granular bio fungicide formulations with the active ingredient of T. virens endophytes and to obtain the best biofungicide preparations to control G. boninense and improve the quality of oil palm seeds. The research was conducted at the experimental farm and plant disease laboratories, Faculty of Agriculture, the University of Riau from February 2017 to September 2018. The research was carried out experimentally using a completely randomized design (CRD). The treatment tested was the Trichoderma virens formulation F0 = Control without treatment, F0- = Control negative (Ganoderma inoculant), F0 + = Positive control (15 ml Trichoderma virens inoculant + Ganoderma inoculant), F1 = 15 ml Trichoderma virens inoculant +100 g sago starch + 25 peat peat +25 g tapioca starch, F2 = 15 ml Trichoderma virens inoculant +100 g palm frond flour + 25 sago dregs + 25 g tapioca flour, F3 = 15 ml Trichoderma virens inoculant + 100 g solid +25 g talc + 25 g flour tapioca, F4 = 15 ml Trichoderma virens inoculant + 100 g cocoa shell flour + 25 g zeolite + 25 g tapioca flour. The parameters observed were the intensity of the attack of the disease, seed height, weevil diameter, number of fronds, root volume, seed dry weight, and sprout ratio. uk root. The obtained data were statistically analyzed using variant fingerprints. The average results of the analysis were continued with the BNJ test at the 5% level. F4 treatment showed the best granular formulation for the growth and development of T. virens endophytes, inhibiting the infection of Ganoderma sp. Attack. on seedlings and better seed growth on observations of seed height, root volume, number of midribs, root crown ratio, and dry weight of oil palm seedlings.
PRODUKSI PIGMEN JINGGA Penicillium sp. LBKURCC27 PADA MEDIA CAIR DENGAN VARIASI pH DAN SUMBER NITROGEN, SERTA POTENSINYA SEBAGAI PEWARNA TEKSTIL Gaol, Fuji Randa Lumban; Ramadhan, Gilang; Sakinah, Alifia Andra; Fitri, Nur Annisa; Gurning, Esther Angelita; Nugroho, Titania Tjandrawati
Akta Kimia Indonesia Vol 9, No 1 (2024)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25493736.v9i1.20626

Abstract

Penggunaan pigmen warna sintetis yang marak di industri tekstil, dikhawatirkan akan menimbulkan dampak lingkungan selama produksi maupun pengaplikasiannya. Limbah yang dihasilkan dari pigmen warna sintesis sulit didegradasi dan cenderung bersifat karsinogenik. Pigmen warna alami yang dihasilkan jamur Penicillium dapat menjadi solusi alternatif untuk mengatasi dampak lingkungan yang dihasilkan dari penggunaan pigmen warna sintesis tersebut. Penelitian ini bertujuan untuk menggali potensi isolat lokal Penicillium sp. LBKURCC27 sebagai sumber pigmen warna jingga alami dengan variasi pH media dan sumber nitrogennya. Penelitian dilakukan dengan memproduksi pigmen menggunakan isolat lokal Penicillium sp. LBKURCC27 pada media PDB dengan variasi pH (pH 4,5 hingga 7,5) dan variasi sumber nitrogennya (urea, tepung kedelai dan ekstrak ragi) pada suasana gelap dan statis selama 40 hari.  Hasil penelitian menunjukkan bahwa media produksi pada pH 5,5 menghasilkan jumlah pigmen yang secara signifikan (p<0,05) lebih tinggi dibandingkan dengan pH lainnya. Sumber Nitrogen berupa urea akan menghasilkan jumlah pigmen yang secara signifikan (p<0,05) lebih tinggi bila dibandingkan dengan sumber nitrogen berupa ekstrak ragi dan tepung kedelai. Hasil ekstraksi diuji menggunakan spektrofotometri UV-Vis untuk mengetahui panjang gelombang optimumnya sehingga diperoleh panjang gelombang 511 nm sebagai panjang gelombang optimum. Uji pewarnaan dilakukan dengan metode mordanting menggunakan mordant besi sulfat dan tawas yang menghasilkan kain berwarna coklat hingga jingga. Analisis kolorimetri juga dilakukan pada kain yang telah diwarnai sehingga diperoleh koordinat kromatis primer berupa RGB. Hasil pewarnaan kain dengan mordant besi sulfat memiliki warna yang lebih merata. Kain sutra memiliki warna yang lebih gelap dan lebih merata dibandingkan pewarnaan pada kain katun.
Produksi Biokompos Melalui Teknik Mini Biopori di dalam Wadah Bekas Ramah Lingkungan Untuk Tanaman Hortikultura Dahliaty, Andi; Sophia, Halida; Azlina, Nur; Siregar, Siti Saidah; Susilawati, Susilawati; Nugroho, Titania Tjandrawati
Unri Conference Series: Community Engagement Vol 6 (2024): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.6.247-253

Abstract

Mini biopori is an innovative and efficient technique for producing biocompost. This technique utilizes used containers that are environmentally friendly, allowing the community to independently process household organic waste. In addition to reducing waste, this technique also produces fertilizer that is beneficial for horticultural plants. The purpose of this community service activity is to provide knowledge and insights to the community about the application of mini biopori technology in used containers for horticultural plant production, enhance the community's skills in applying mini biopori technology, utilize the biocompost produced from biopori infiltration holes for planting horticultural crops, and assist the community from an economic aspect (increasing community income) by using simple and inexpensive materials to produce high-value horticultural plants. This activity is conducted in Sungai Berbari Village, Pusako District, Siak Regency. The methods of this community service consist of several stages, including preparation, training through lectures and discussions, practical implementation of the mini biopori technique, and evaluation, which considers the conditions before and after the training activities. The results of this activity indicate that the participants have a better understanding of the biocompost production process and the benefits of the mini biopori technique. All participants (100%) considered the mini biopore technique training to be very beneficial, indicating that this training has successfully provided added value to the participants. A total of 70% of the participants showed an interest in applying the mini biopore technique in their homes. The mini biopore technique is considered effective in managing organic waste, producing beneficial fertilizer, and has the potential to reduce dependence on chemical fertilizers. Additionally, implementing this technique can lower agricultural production costs, increase crop yields, and contribute to long-term economic well-being in the community.
Co-Authors - Miranti Adja Muhammad Alsan Shaf Andhra Syatapahana Andi Dahliaty Andi Dahliaty Andi Dahliaty Annisa Fitri Ayu Octa Mellani Bustari Hasan Citra Hardiyanti Desmelati Desmelati Dian Iriani Dian Novita Sary Edy Fachrial Feliatra Fifi Puspita Fifi Puspita Fifira Safitri Fitri Rahayu Fitri, Nur Annisa Gaol, Fuji Randa Lumban Gurning, Esther Angelita Halida Sophia Harni Sepriani Hilwan Yuda Teruna Iin Evita Sari Ismawati Ismawati Ismawati ismawati Jati, Afif P Jeky Sasemar Lumban Khairullinas Khairullinas M Andesba Arifin Miranti Miranti Miranti Miranti Miranti Miranti Miranti, - Narasswati, Nungki Nenci, Nuryani Nova Rianti Putri Novia Sellyna Nur Azlina Nuria Wulandari Nurwijayanti Nuryani Nenci Oktavia, Rani Puja, Krisna Puspita Sari Putri Nabilah Putri, Nova Rianti Ramadhan, Gilang Rani Yuniati Sakinah, Alifia Andra Saputra, Rachmad Saryono Saryono Saryono &#039; Saryono Saryono Saryono, M.Or. S.Pd. Jas. Senjavi Rakhmana Sepriani, Harni Seprianto, Seprianto Sila Silalahi Siregar, Siti Saidah Sri Helianty suraya, nabella Susilawati, Susilawati Utama, Panca Setia Wahyuningsih Wahyuningsih Yanti Lim Yanti Yanti Yanti Yanti Yuana Nuralita Yuana Nurulita Yuana Nurulita Yuharmen - Yuharmen Yuharmen Yum Eryanti Yuslina Octavia Zona Octarya Zona Octarya