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All Journal VALENSI Bionatura Indonesian Journal of Cancer Chemoprevention ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Journal of Medicine and Health Chimica et Natura Acta Indonesian Journal of Chemistry Jurnal Agrotek Tropika Molekul: Jurnal Ilmiah Kimia Journal of Health Technology Assessment in Midwifery Odonto dental journal Journal of Medicine and Health
Dikdik Kurnia
Departemen Kimia Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Activity of Superoxide Dismutase Mimic of [Mn(salen)OAc] Complex Compound Non-enzymatically in Vitro Through Riboflavin Photoreduction Yusi Deawati; Djulia Onggo; Irma Mulyani; Iwan Hastiawan; Dikdik Kurnia
Molekul Vol 12, No 1 (2017)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.163 KB) | DOI: 10.20884/1.jm.2017.12.1.294

Abstract

The complex compound of [Mn(salen)OAc] can serve as mSOD and its activity has been determined non-enzymatically in vitro through riboflavin photoreduction. The complex was synthesized from Mn(OAc)2.4H2O and H2salen. Based on the elemental analysis, the C=56.69%; H=4.21%; and N=7.52% content are corresponding to the chemical formula of MnC18H17N2O4. The functional groups and ionic species in the complex have been analyzed by infrared spectroscopy and ESI-MS. SOD activity was determined by mixing complex at various concentrations with riboflavin and nitroblue tetrazolium (NBT), then the mixture was lighted with 20 Watt tungsten lamp for 15 minutes in a closed box. Then the reduced NBT absorptions were measured at λ 560 nm. The difference of absorbance between standard and sample solutions (without and with riboflavin, respectively) was multiplied by 100% to obtain %inhibition of each various sample concentration against NBT. SOD activity was obtained from IC50 data defined as a 50% inhibition of the plot curve of % inhibition to the concentration of the complex. The result obtained for this compound is IC50 = 2.7 ± 0.05 µM as well as enzymatic method. Therefore, this method can be used to determine the SOD activity by giving more stability and accuracy of IC50 value.
The prospects of complementary treatment using Red Piper betle (Piper crocatum) against leucorrhoea: an empirical study Siswina, Tessa; Kurnia, Dikdik; Rustama, Mia Miranti; Sumiarsa, Dadan
Journal of Health Technology Assessment in Midwifery Vol. 6 No. 2 (2023): November
Publisher : Universitas Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31101/jhtam.3232

Abstract

One of the main issues for women in the reproductive age range is leucorrhoea, which causes a woman ongoing distress, and occasionally be the first sign of several of the main gynecological disorders. The widespread use of fluconazole antibiotics has caused Candida sp to become resistant. Alternative non-pharmacological treatments based on natural products are needed, especially P. crocatum which has antioxidant, antifungal, and anti-inflammatory activities. This study analyzed the empirical study of P. crocatum used against leucorrhoea due to C. albicans. The empirical study presented that vaginal discharge was reduced after using P. crocatum formulations. The infection parameter of vaginal pH level, the intensity of pain, and itching were significantly recovered with p values of 0.008, 0.000, and 0.001, respectively, while the reducing C. albicans colonies was 0.001. P. crocatum decoction formulation is effective in reducing vaginal yeast infections in women, close to the results of the positive control, ketoconazole.
Potential Natural Antibacterial Agent for P. gingivalis Periodontitis Infection: A Comprehensive Review of Source, Structure and Mechanism actions Amin, Meiny Faudah; Ariwibowo, Taufiq; Putri, Salsabila Aqila; Kurnia, Dikdik
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.146-158

Abstract

The pathogenic bacteria P. gingivalis grows in the oral cavity. This bacterium could attack immune system which lead to inflammation of most tissues. P. gingivalis can cause a variety of serious and dangerous condition such as periodontitis, Alzheimer, rheumatoid arthritis, diabetes, and pneumonia. Antibiotics have been used for years as a treatment against this bacterium, like metronidazole, amoxicillin, and clindamycin, however, P. gingivalis is reported to be resistant to these antibiotics, thus exploration to discover alternatives has been demanded. Natural product compounds are known to have antibacterial activity and cause fewer side effects. Turmeric, eucalyptus, and several other plants have been reported to have antibacterial activity against P. gingivalis with a MIC of 1g/mL from an ethyl acetate leaf extract of eucalyptus. Decent antibacterial activity could be used as a reference to discover new drugs as alternatives against P. gingivalis
Extraction and Characterization of Phenolic Compounds from the Stem Bark of Sonneratia caseolaris (Lythraceae) and Their Potential Antibacterial Activity Harizon, Harizon; Kurnia, Dikdik; Sumiarsa, Dadan; Herlina, Tati; Sinaga, Siska Elisahbet; Shiono, Yoshihito; Azmi, Mohamad Nurul; Supratman, Unang
Indonesian Journal of Chemistry Vol 25, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.98976

Abstract

The ethyl acetate fraction separated from the stembark of Sonneratia caseolaris retrieved three phenolic compounds, including quercetin-3-O-glucoside (1), quercetin (2), and 1-O-(2,4-dihydroxybenzoyl)-β-D-glucopyranose (3). For the first time, compounds 1 and 3 were discovered from Sonneratia genus. Data from various spectroscopic techniques, including mass spectroscopy and one- and two-dimensional NMR, were used to identify their chemical structures. Antibacterial activity has also been assessed for all compounds against Staphylococcus aureus ATCC 25175 and Streptococcus mutans ATCC 6538. Compounds 1–3 displayed varying levels of antibacterial activity against S. aureus and S. mutans. However, all compounds exhibited lower efficacy compared to the control, with their minimum inhibitory concentration (MIC) values ranging from 71.25 to greater than 100 µg/mL. This study provides a foundation for optimizing S. caseolaris phenolic compounds as antibacterial agents and highlights the need for comparative studies within the Sonneratia genus to identify potent bioactive candidates through structural modification or synergistic approaches.
Antibacterial Effect of Lime (Citrus aurantifolia) Peel Extract in Preventing Biofilm Formation Jeffrey Jeffrey; Mieke H Satari; Dikdik Kurnia
Journal of Medicine and Health Vol 2 No 4 (2019)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28932/jmh.v2i4.1841

Abstract

The routine and long term use of chemicals to maintain oral and dental health have the potency to result in the emergence of side effects; therefore another strategy is needed as an alternative such as using antimicrobial agents extracted from plants. The purpose of this study is to review the effectiveness of lime (Citrus aurantifolia) peel extract as an antibacterial in preventing biofilm formation. Biofilm is a component consisting of bacteria in a self-produced polymeric matrix, attached to an inert surface, alive, and can survive because of its ability to capture nutrients and withstand adverse environmental conditions. Lime peel contains flavonoids which are the largest group of polyphenol compounds that can work as antioxidants and antibacterial by denaturing bacterial cell proteins and damaging bacterial cells. Flavonoids can also inhibit glucosyltransferase (GTF) activity of Streptococcus mutans to prevent biofilm formation. Lime peel extract inhibits the formation of the activity of the enzyme Streptococcus mutans. As a conclusion lime peel extract contains compounds with therapeutic potential and has the effect of inhibiting the formation of the activity of the enzyme Streptococcus mutans so that it can be used to inhibit the formation of biofilms. Keywords: antibacterial, biofilm, Citrus aurantifolia
Antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers towards Streptococcus sanguis ATCC 10566 Fatriadi, Fajar; Kurnia, Dikdik; Satari, Mieke Hemiawati
Padjadjaran Journal of Dentistry Vol 30, No 3 (2018): November 2018
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.849 KB) | DOI: 10.24198/pjd.vol30no3.20002

Abstract

Introduction: Streptococcus sanguis is an initial cause of dental plaque formation which is the initial cause of caries. One of the preventive treatments can be done by using the mouthwash containing antibacterial substances. Along time, natural remedies are proven to be having more antibacterial properties. Ant-plant (Myrmecodia pendens Merr. & Perry) tubers are types of epiphytic plant that grows in many parts of Papua, with many health benefits, and are known to contain flavonoids, tannins, and tocopherols. This study was aimed to determine the antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers on the growth of Streptococcus sanguis ATCC 10566. Methods: The ant-plant tubers were extracted with soxhletation method using the methanol solvent. The fractionation was then performed using ethyl acetate to obtain the ethyl acetate fraction. Result: The phytochemical test showed that the ethyl acetate fraction of the ant-plant contained phenolics, tannins, flavonoids, and terpenoids. The bacterial test in this study was using the microdilution method using the ELISA Reader by measuring the Minimum Inhibitory Concentration (MIC) of ethyl acetate fraction of ant-plant tubers towards Streptococcus sanguis with positive control was using the chlorhexidine gluconate as the golden standard medication. The ethyl acetate fraction of ant-plant tuber had an antibacterial effect towards Streptococcus sanguis in the MIC of 31.25 ppm, while chlorhexidine gluconate was 0.49 ppm. Conclusion: The ethyl acetate fraction of ant-plant had a lower antibacterial activity compared to chlorhexidine gluconate on the growth of Streptococcus sanguis ATCC 10566.Keywords: Ant-plant tubers, Myrmecodia pendens Merr. & Perry, antibacterial activity, Streptococcus sanguis ATCC 10566.
The Effect of Moringa oleifera's antibacterial and antibiofilm properties against Fusobacterium nucleatum and Staphylococcus aureus: an experimental study Fernando, Theodorus Aldo; Amin, Meiny Faudah; Ariwibowo, ⁠Taufiq; Binartha, Ciptadhi Oka; Kurnia, Dikdik
Padjadjaran Journal of Dentistry Vol 37, No 2 (2025): July 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol37no2.59343

Abstract

Introduction: Fusobacterium nucleatum and Staphylococcus aureus, both associated with pulp and periapical diseases, must be effectively eliminated during irrigation. Natural agents are generally more biocompatible and less harmful to bodily tissues, making them a promising alternative to chemical irrigants such as sodium hypochlorite. Fractions of Moringa oleifera have demonstrated potential as an alternative irrigant due to their antibacterial and antibiofilm properties. This study aimed to analyze the antibacterial and antibiofilm efficacy of Moringa oleifera fractions against Fusobacterium nucleatum and Staphylococcus aureus. Methods: The tube dilution method was used for antibacterial tests, while the biofilm assay method measured the optical density (OD) in a 96-well plate to evaluate antibiofilm effects against Fusobacterium nucleatum ATCC 25586 and Staphylococcus aureus ATCC 25923. Moringa oleifera fractions were tested at 20%, 40%, 60%, and 80% concentrations. A one-way ANOVA test was applied for normally distributed data, and the Kruskal Wallis test was used for non-normally distributed data. Post-hoc analyses were conducted to determine significant differences between groups, with the significance level set at 0.05. Results: The antibacterial test revealed statistically significant differences between Moringa oleifera groups in eliminating Fusobacterium nucleatum (p=0.003, p<0.05) and Staphylococcus aureus with p value (p=0.001, p<0.05). The antibiofilm test also showed statistically significant differences among the concentration groups for both bacteria. Conclusion: The antibacterial and antibiofilm effectiveness of Moringa oleifera’s fractions against Fusobacterium nucleatum and Staphylococcus aureus, supporting its potential as an alternative irrigant.
The antibacterial effect of β-pinene derived from Citrus aurantifolia peel against oral Streptococcus mutans ATCC 25175 Julaeha, Euis; Herlina, Tati; Nurzaman, Mohamad; Mayanti, Tri; Kurnia, Dikdik; Sari, Elizabeth Fitriana
Padjadjaran Journal of Dentistry Vol 33, No 1 (2021): March 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no1.29200

Abstract

Introduction: Streptococcus mutans has been known to play a major role in dental caries development. This tooth decay is the most common oral disease affecting people in the world. Hence, discovering the new herbal antibacterial agent seems to become more promising yet challenging. One of natural antibacterial source is the peel of Citrus aurantifolia, as it may contain antibacterial active compounds against pathogenic oral microbes. This study was aimed to isolate antibacterial compound from essential oil of Citrus aurantifolia and to analyse its antibacterial activity against oral Streptococcus mutans ATCC 25175. Methods: Type of research was laboratory experimental. The essential oil was isolated from peel of Citrus aurantifolia by hydro-distillation technique and further isolation of antibacterial compounds was conducted by combination column chromatography using organic solvent, and the structure was determined by UV-Vis, infrared (IR), 1D NMR of 1H-, 13C-NMR and DEPT 135° in CDCl3 spectrometers methods. The antibacterial activity was tested against Streptococcus mutans ATCC 25175 using the Kirby-Bauer method. Results: 0.75% yield was obtained from the essential oil of peel Citrus aurantifolia and after purification, an antibacterial compound was identified as β-pinene with the molecular formula C10H16. Furthermore, the β-pinene from peel of Citrus aurantifolia exerted inhibition growth against Streptococcus mutans at concentration of 2000, 1000, and 500 ppm by showing respectively, inhibition values of 13.0, 11.9, and 11.6 mm on paper disk. Conclusion: In the present study, the essential oil of Citrus aurantifolia peel is proven to contain prospective antibacterial compound (β-pinene) that potentially can be used as natural antibacterial alternative choice to manage oral disease associated with Streptococcus mutans infection.
Antibacterial activity of strawberry fruit extract against Streptococcus sanguinis ATCC 10556) Nurdin, Denny; Dharsono, Hendra Dian Adhita; Fatriadi, Fajar; Sahara, Elvi; Adang, Rahmi Alma Farah; Gondowidjojo, Yolanda Dwiutami; Kurnia, Dikdik
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.37043

Abstract

Introduction: Streptococcus sanguinis is a facultative anaerobic Gram-positive bacteria known as a pioneer that plays a role in creating the biofilm in the oral cavity. Strawberry fruit (Fragaria x ananassa) is an edible fruit widely used for the study as their active compound synergy to improve health. This study aims to analyse the antibacterial activity of strawberry fruit against S. sanguinis (ATCC 10556). Methods: An explorative study was conducted with high concentration methanol extract from strawberry fruit. Antibacterial activity was tested on the methanol extract; afterwards, the extract was fractionated and divided into three fractions: water, ethyl acetate, and hexane. Zone of inhibition was used to assess the most effective fraction among those three, then continued by testing for Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: Inhibitory zone of the ethyl acetate fraction from strawberry fruit 1%, 2%, 3% ,4% and 5% sequentially were 7.3 mm,10.2 mm, 12.3 mm, 16.3 mm and 16.1 mm. Ethyl acetate fraction of 4% was the most effective to create the zone of inhibition with a size of 16.3 mm compared to the others. The Minimum Inhibitory Concentration (MIC) value was 0.25%. It was obtained by diluting a 4% ethyl acetate fraction on a microplate. The Minimum Bactericidal Concentration (MBC) was 2%. Conclusion: Ethyl acetate fraction was an effective fraction from strawberry fruit and had antibacterial activity against S. sanguinis with the inhibitory zone in the concentration of 4%, MIC of 0.25%, and MBC of 2%.
PHENYLALANINE-INDUCED MODULATION OF CALLUS CHARACTERISTICS AND SECONDARY METABOLITE ACCUMULATION IN Ocimum basilicum L. UNDER IN VITRO CONDITIONS Suminar, Erni; Mubarok, Syariful; Budiarto, Rahmat; Yulianto, Fiky; Nuraini, Anne; Yuniarti, Anni; Kusumadewi, Vira; Meliansyah, Rika; Kurnia, Dikdik; Julaeha, Siti
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.11044

Abstract

Basil (Ocimum basilicum L.) is widely used in traditional medicine due to its rich content of phenolic and flavonoid compounds. However, the natural production of these metabolites is limited. Callus culture offers a controlled method to enhance their accumulation, with medium composition, particularly precursor supplementation, playing a critical role. Phenylalanine, an aromatic amino acid, is a key precursor in the biosynthesis of phenolics and flavonoids via the shikimate pathway. This study aimed to evaluate the effect of phenylalanine on callus growth and the accumulation of total phenolic and flavonoid compounds in basil. The experiment was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Universitas Padjadjaran, using Murashige and Skoog (MS) medium with phenylalanine concentrations of 0, 1.3, 1.6, and 2 g.L-1, each replicated six times. Results showed that phenylalanine treatments caused brown coloration, compact callus texture, and inhibited growth, as indicated by reduced fresh and dry weights. The 1.6 g.L-1 treatment produced the highest total phenolic content, while the 2 g.L-1 treatment yielded the highest flavonoid content. These findings suggest that phenylalanine can enhance secondary metabolite accumulation in basil callus, although it may suppress biomass growth.
Co-Authors . Horizon Ambrosius Purba Amir M. Suruwaky ANNE NURAINI Anni Yuniarti Ariwibowo, ⁠Taufiq Azmi, Mohamad Nurul Bashari, Muhammad Hasan Betry Pujiastuti Binartha, Ciptadhi Oka Boima Ramses Situmeang Budi Setiabudiawan Cut Soraya Dadan Sumiarsa Danny Hilmanto Darma, Joshua Denny Nurdin Dharsono, Hendra Dian Adhita Diana Rakhmawaty Djulia Onggo Djulia Onggo Dudi Aripin Elizabeth Fitriana Sari Elline, Elline Erni Suminar Euis Julaeha Fajar Fatriadi Fauzi, Puad Fernando, Theodorus Aldo Gondowidjojo, Yolanda Dwiutami Hafiz, Ersanda Harizon Harizon Hendra Dian Adhita Hendra Dian Adhyta Dharsono Huda, Fathul Indah Permata Yudha Irma Mulyani Irma Mulyani Iwan Hastiawan Iwan Hastiawan Jamaludin Al-Anshori Jeffrey Jeffrey Kadarusman Kiki Maesaroh Komariah Komariah Kusumadewi, Vira Lilis Siti Aisyah, Lilis Siti Meilinah Hidayat - Meiny Faudah Amin Mia Miranti Rustama Mieke H Satari Mieke H Satari Mieke H Satari Mieke H. Satari Mieke Hemiawati Satari Mohamad Nurzaman Muchtan Sujatno - Murtihapsari . Nugraha Sutadipura - Putri, Salsabila Aqila Putri, Tenny Qomarilla, Nurul Rahmat Budiarto Rahmi Alma Farah Adang, Rahmi Alma Farah Ratna Annisa Utami RIKA MELIANSYAH Rismawati Rismawati Sahara, Elvi Sahiratmadja, Edhyana Kusumastuti Santhy Wyantuti Satari, Mieke Hermiawati Setiawan - Shiono, Yoshihito Sinaga, Siska Elisahbet Siswina, Tessa Siti Julaeha, Siti SYARIFUL MUBAROK Tati Herlina Tati Herlina Taufiq Ariwibowo Tri Mayanti Unang Supratman Yoshihito Shiono Yuli Ainun Rosidah Yuliana, Trisna Yulianto, Fiky Yuniarti, Eveline Yusi Deawati Yusi Deawati Yusrin, Yolanda Charmenia Nadine Zein, Muhammad Ihda H. L. Zulfan M. Alibasyah