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All Journal Bioscience AL KAUNIYAH Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Martabe : Jurnal Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN TAMBUSAI Pelita Eksakta Jurnal Biogenerasi Journal of halal product and research (JHPR) 3BIO: Journal of Biological Science, Technology and Management Jurnal Jeumpa Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Promotor: Jurnal Mahasiswa Kesehatan Masyarakat Tropical Genetics Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi
Achyar, Afifatul
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang, Padang Indonesia
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DETEKSI PENCEMARAN AIR DANAU TALANG DAN DANAU TAMBAU NAGARI KAMPUNG BATU DALAM KECAMATAN DANAU KEMBAR KABUPATEN SOLOK SECARA SEDERHANA Yusni Atifah; Afifatul Achyar; Gilang Amanda; Hafizh Alza Afra; Threo Wanda Marten
Eksakta : Jurnal Penelitian dan Pembelajaran MIPA Vol 8, No 1 (2023): Eksakta : Jurnal Penelitian dan Pembelajaran MIPA
Publisher : Fakultas Keguruan Dan Ilmu Pendidikan, UM-Tapsel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/eksakta.v8i1.105-111

Abstract

Danau Tambau dan Danau Talang merupakan danau yang berada di Jorong Air Tawa Utara, Nagari Kampung Batu Dalam, Kecamatan Danau Kembar, Kabupaten Solok. Kedua danau ini dikelola oleh masyarakat setempat dalam Kelompok Sadar Wisata (Pokdarwis). Begitu pentingnya kedua danau ini bagi masyarakat sekitar, maka penting dilakukan konservasi dan perlindungan agar kualitas danau ini tetap terjaga. Sebagai upaya awal dalam langkah konservasi maka perlu dilakukan deteksi awal terjadinya pencemaran. Deteksi pencemaran secara sederhana penting untuk dilakukan oleh masyarakat dalam mendeteksi adanya pencemaran sedini mungkin sebagai langkah preventif dalam konservasi perairan. Metode yang digunakan adalah observasi dan eksperimen. Prosedur deteksi pencemaran dilakukan melalui tiga jenis analisis secara fisika, biologi dan kimia sederhana. Hasil penelitian menunjukkan bahwa air danau tambau terindikasi mengalami pencemaran melalui uji fisika, kimia dan biologi sederhana sehingga air danau tambau tidak bisa digunakan sebagai sumber air minum. Air danau talang dan kontrol menunjukkan hasil yang relatif sama dengan kualitas air yang cukup baik dan belum terindikasi adanya pencemaran.
EDUKASI JAJANAN SEHAT KEPADA SISWA SD DALAM MENGHADAPI ERA NEW NORMAL Yusni Atifah; Afifatul Achyar; Rijal Satria; Violita Violita; Helsa Rahmatika; Jalilah Azizah
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 6, No 1 (2023): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v6i1.213-217

Abstract

Anak usia sekolah memerlukan asupan energi dan zat gizi untuk mendukung pertumbuhan dan juga prestasi belajar anak di sekolah. Salah satu sumber asupan energi dan zat gizi bagi anak sekolah bersumber dari pangan jajanan. Anak usia sekolah sebagai generasi penerus bangsa merupakan investasi bagi suatu bangsa. Kualitas anak-anak pada masa sekarang akan menentukan kualitas suatu negara di masa depan. Edukasi keamanan pangan jajanan perlu dilakukan sejak dini secara sistematis dan juga berkelanjutan sebagai salah satu upaya untuk peningkatan kualitas sumber daya manusia. Pemberian nutrisi yang berkualitas, sehat dan sesuai dengan kuantitas yang seharusnya penting untuk menunjang pertumbuhan perkembangan anak usia sekolah agar optimal. Oleh karena itu, perlu adanya edukasi kepada siswa SD untuk meningkatkan pengetahuan terkait keamanan pangan jajanan dan mengetahui arti simbol-simbol pada bungkus jajanan. Kegiatan ini dilaksanakan pada bulan Juli 2022, dan diikuti oleh 206 peserta dari kelas 3 dan 6 SDN 02 Payakumbuh. Hasil evaluasi kegiatan menunjukkan adanya peningkatan pengetahuan siswa terhadap keamananan jajanan dan memiliki keterampilan dalam mengidentifikasi simbol-simbol pada bungkus jajanan. Kesimpulan untuk kegiatan ini sudah efektif dilaksanakan dan dapat mengedukasi siswa SD dalam  mengetahui keamanan pangan jajanan.
Polymerase chain reaction (PCR) primer design to identify SNP rs7901695 transcription factor 7 like 2 (TCF7L2) Elsa Badriyya; Afifatul Achyar
Bioscience Vol 7, No 1 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202371122575-0-00

Abstract

ABSTRACT. Transcription Factor 7 Like 2 (TCF7L2) is a gene that produces a protein that controls the expression and function of several incretin hormones. One of the primary activities of incretin hormones is to increase insulin production, which is important in glucose and energy homeostasis. Single Nucleotide Polymorphism (SNP) in the TCF7L2 gene is reported to be association with Type II Diabetes Mellitus (T2DM). T2DM is featured by persistent hyperglycemia because of the decrease in insulin production, insulin resistance, or both. In patients with diabetes mellitus, chronic hyperglycemia can damage organ systems and cause metabolic abnormalities. SNP rs7901695 TCF7L2 gene has been linked to T2DM in a number of places, including South Asia, Iceland, and the United States. The polymorphism at location 112.994.329 from nucleotide Timine (T) to Cytosine (C) enables the recognition of SNP rs7901695. Polymerase Chain Reaction (PCR) was used to identify the polymorphism. The objective of this research was to generate a precise primer for the PCR recognition of the SNP rs7901695 in the TCF7L2 gene. The research's methodology comprises DNA isolation, primer designing with Geneious, target amplification with PCR, and DNA sequencing for bioinformatic analysis. As a result of the study, four primers for the SNP rs7901695 TCF7L2 gene have been developed. The reaction obtained two fragments, sized 177 and 367 bp. The primers used were rs7901695-F, rs7901695-R, rs7901695-F(C), and rs7901695-R(T), which were used to detect the T allele.  ABSTRAK. Transcription Factor 7 Like 2 (TCF7L2) adalah gen yang menghasilkan protein yang mengontrol ekspresi dan fungsi beberapa hormon inkretin. Salah satu aktivitas utama hormon inkretin adalah meningkatkan produksi insulin, yang penting dalam homeostasis glukosa dan energi. Single Nucleotide Polymorphism (SNP) pada TCF7L2 gene dilaporkan adanya hubungan dengan Diabetes Mellitus Tipe II (DMT2). DMT2 ditandai dengan hiperglikemia persisten karena penurunan produksi insulin, resistensi insulin, atau keduanya. Pada penderita diabetes melitus, hiperglikemia kronis dapat merusak sistem organ dan kelainan metabolisme. Gen SNP rs7901695 TCF7L2 dilaporkan memiliki asosiasi dengan DMT2 di sejumlah daerah, termasuk Asia Selatan, Islandia, dan Amerika Serikat. SNP rs7901695 ditandai dengan polimorfisme pada lokasi 112.994.329 dari nukleotida Timin (T) menjadi Sitosin (C). Metode yang digunakan untuk mengidentifikasi polimorfisme adalah Polymerase Chain Reaction (PCR). Penelitian ini bertujuan untuk menghasilkan primer yang tepat untuk pengenalan PCR terhadap SNP rs7901695 pada gen TCF7L2. Metodologi penelitian meliputi isolasi DNA, perancangan primer dengan aplikasi Geneious, amplifikasi target dengan PCR, dan sekuensing DNA untuk analisis bioinformatik. Sebagai hasil penelitian, empat primer untuk deteksi gen SNP rs7901695 TCF7L2 telah dikembangkan. Dua fragmen, berukuran 177 dan 367 bp, diperoleh dari reaksi tersebut. Primer yang digunakan adalah rs7901695-F, rs7901695-R, rs7901695-F(C), dan rs7901695-R(T), yang digunakan untuk mendeteksi alel T.
Specific primer design and optimization of annealing temperature for amplification gene peroxidase (POD) in Oryza sativa L. Nella Fauziah; Afifatul Achyar; Zulyusri Zulyusri; Yusni Atifah; Linda Advinda; Violita Violita
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.122972

Abstract

Peroksidase (POD) merupakan enzim antioksidan yang memiliki beragam fungsi dalam siklus hidup tanaman, salah satunya adalah sebagai pertahanan dalam melawan ROS dengan mengkatalisis konversi H2O2 menjadi udara dan O2 . Kemampuan aktifitas enzim POD dalam mengatur kandungan H2O2 memungkinkan enzim tersebut dapat mempertahankan tanaman dari cekaman. Metode yang dapat digunakan untuk amplifikasi gen POD salah satunya yaitu kuantitatif reverse transcription- PCR (qRT-PCR). Metode ini memerlukan beberapa komponen penting salah satunya yaitu primer ( forward dan reverse ). Primer yang digunakan dalam amplifikasi gen harus spesifik terhadap gen target sehingga dapat mengenali dan menempel pada gen target yang diinginkan. Penelitian ini bertujuan untuk mendesain primer yang sesuai untuk amplifikasi gen POD menggunakan teknik qRT-PCR. Primer dirancang menggunakan perangkat PrimerQuest. Primer yang telah dirancang kemudian dianalisis untuk spesifikasinya dengan geneious prime . Kemudian spesifikasi primer di cek menggugakan primer BLAST. Hasil desain primer dengan kriteria terbaik untuk amplifikasi gen POD yaitu Forward POD 5'-AAATGCGTCGATCTACTGTACCT-3' dan Reverse POD 5'-GTGTTGAAAATGGCAATAAACCGG-3'
Rice Growth Response to Drought Simulation Treatment Using PEG Annisa Khaira; Zulyusri Zulyusri; Afifatul Achyar; Dwi Hilda Putri; Yusni Atifah; Violita Violita
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.122674

Abstract

Rice is the main food source for Indonesians. The demand for rice to meet people's needs increases every year due to population growth and efforts to improve nutrition by the community. One of the problems that can affect rice production is drought. Drought is an environmental condition when plants do not get enough water to grow and develop optimally, which can cause a decrease in rice production. To find out how rice growth responds to drought, a study was carried out by giving drought simulation treatments using polyethylene glycol (PEG) on several rice varieties. This study used a completely randomized design that was arranged in a factorial manner with two factors. The first factor was the rice varieties (Harum, Situbagendit, Rosna) the second factor was 0% and 20% PEG concentration. The data obtained were then analyzed statistically using a two-way ANOVA test, and if the results were significantly different, then proceed with Duncan's test at the 5% level.The results showed that the drought simulation treatment had a negative effect on rice growth. Drought simulation treatment using 20% PEG resulted in a decrease in Kadar air relatif (KAR), root length, plant height, and root dry weight of rice. The highest decrease in KAR was found in the sensitive rice variety (Rosna), which was 43.42%. The highest average root length (7.99 cm) was on the sitabaendit variety, and the lowest (5.61) was on the rosna variety. The highest average crown height (17.32 cm) and the lowest (6.61) were on the rosna variety.
Multiple Alignment and Primer Design for Groups of Barnacle Organisms and Amphibalanus amphitrite as Biofouling Markers Muhammad Farikh; Rahmad Wanizal Pastha; Rezeki Rival Alridho; Aura Zahra Nafisah; Bintang Fadhil Ramadhan; Afifatul Achyar
Bioscience Vol 7, No 2 (2023): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v7i2.125392

Abstract

Biota pengotor atau biofouling adalah organisme hidup yang menempel pada suatu substrat. Dampak yang ditimbulkan oleh aktivitas biofouling biota adalah oleh karena sifatnya yang korosif sehingga dapat menyebabkan kelumpuhan pada bangunan instalasi. Untuk memfasilitasi instalasi dan kestabilan biodiversitas, peneliti mencoba menghadirkan environmental DNA (e-DNA) sebagai sampel lingkungan tanpa membunuh dan mengisolasi suatu organisme tersebut dengan memanfaatkan sampel lingkungan. Penelitian ini bertujuan untuk memperoleh primer universal dan spesifik dari COX1 yang dapat digunakan untuk mengidentifikasi biofouling kelompok Barnacle yang dirancang secara in silico. Sekuen organisme target yang digunakan dalam penelitian ini adalah DNA mitokondria dari Amphibalanus amphitrite, Amphibalanus reticulatus dan Semibalanus balanoides dengan nomor aksesi NC_024525, NC_071896 dan NC_039849.. Sekuen gen COX1 yang diperoleh disimpan dalam format FASTA untuk digunakan lebih lanjut di dalam proses desain primer menggunakan Geneious Prime. Penentuan daerah lestari ditentukan dengan Geneious Prime dalam tahapan multiple alignment. Sekuen dengan kriteria primer spesifik terbaik untuk COX1 A.amphitrite didapatkan dengan panjang 20-22 basa dan ukuran amplikon 910 bp. Urutan basa primer foward dan reverse adalah 5'-GAGCTGAACTTGGTCAACCG-3' dan 5'-GCTCAAAGAAGAGGAGGGCTAT-3'. Sekuen dengan kriteria primer universal kelompok barnacle terbaik untuk COX1 didapatkan dengan panjang masing-masing 24 basa dan ukuran amplikon 1.270 bp. Urutan basa primer foward dan reverse adalah 5'-GACTTCTACCGTTAATGTTAGGAG-3''dan 5'-CTATGGTAATGAGGAGGAGTAGTG-3'. Hasil desain memenuhi syarat kriteria primer yang baik sehingga kandidat primer hasil desain dapat digunakan untuk proses PCR.
Design of primer Ipomoea batatas chloroplast gene matK S. Syamsurizal; Ardi Ardi; Des M; Resti Fevria; Yusni Atifah; Elsa Badriyya; Afifatul Achyar
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sweet potato varieties (Ipomoea batatas) Pucuk Hitam Panyalaian and Madu Pucuk Hitam Panyalaian have the potential to be a superior commodity in West Sumatra, but the information of genetic diversity is very limited. Data on the diversity of sweet potato germplasm is beneficial in the selection of plants to obtain superior cultivars in plant breeding. Aim: The aim of the study was to find specific primers for the study of sweet potato genetic markers using Ipomoea batatas chloroplast genes matK. Methods: DNA extraction from sweet potato young leaves; designing forward and reverse primers Ipomoea batatas chloroplast genes matK; PCR; sequencing; bioinformatics analysis and species identification by comparing the NCBI database. Results: Plant DNA barcoding PCR using designed primers matK were successfully resulting single DNA band in different amplicon size in some samples. This indicating that the designed primers used were able to distinguish variation in one species.
Primer design, in silico PCR and optimum annealing temperature for Escherichia coli detection in refillable drinking water samples Afifatul Achyar; Annisa Irna Putri; Dwi Hilda Putri; Yuni Ahda
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Refill Drinking Water Depots (DAMIU) in the community are easy to find at affordable prices, which is a concern in the feasibility of refill drinking water quality. E.coli is one of the pathogenic bacteria present in drinking water that has poor quality. When it enters the body it can cause symptoms of diarrhea, fever, vomiting and others. The purpose of this study was to create a specific primer for E.coli that can be used to detect E.coli and determine the optimum temperature of primer annealing. Researchers do a new primer design because the existing design does not necessarily produce the same results due to various factors of different experimental conditions. The primer design was carried out on an in silico-based and had to meet the criteria for a good primer because it would be used in vitro. E.coli gene sequences was aligned with Shigella sp. using Pairwise Alignment. Primer candidates were analyzed using NCBI's Primer3 and Geneious Prime tools. The result is that the first primer pair is forward 5'–ATGCAGTGGTTCCTTATCTCACA-3' reverse 5'- ATCCTTAATGGCACTGCGCT-3', amplifying the amplicons along 417 bp in the yraJ gene. The second primer pair, forward 5'-CAGAACGTTTTTCATTCAGCAGG-3' reverse 5'-GCCACTACCAGATCGAGTCA-3' amplifies the 573 bp amplicon in the rne gene. The optimum annealing temperature for both pairs of primers was 59.5oC.
Optimization of Specific PCR Conditions for Cows (Bos taurus) in Rendang Samples for Molecular-Based Halal Tests Nurfadillatun Nisa Wijaya; Sisca Alicia Farma; Dwi Hilda Putri; Afifatul Achyar
Tropical Genetics Vol. 2 No. 1 (2022):
Publisher : Genetikawan Muda Indonesia

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Abstract

Rendang is one of the popular foods in Indonesia made from beef as the main ingredient, which has great potential in tourism development. As a country that has a Muslim majority population, this is very influential for the halal tourism industry. Halal tourism is the parent of tourism in accordance with Islamic principles. The issue of halal food is a sensitive issue for the community. The rise of mixing of non-halal ingredients such as pork in processed food products has worried the public, especially for adherents of the Islamic religion. However, this can be detected molecularly using the Polymerase Chain Reaction (PCR) technique which will amplify the DNA of the target gene of the species to be identified. Each gene has a primer with a different concentration and annealing temperature, so that prior to PCR with research samples, it is necessary to optimize the primer concentration and annealing temperature in order to obtain the appropriate PCR composition and conditions so as to obtain optimal PCR results. In this study, optimization of bovine-specific BOS primers that amplify the ND5 gene in mitochondrial DNA was carried out, namely ND5 (NADH dehydrogenase subunit 5). The results showed that the optimum annealing temperature was 48.2ºC, the optimum primary concentration was 0.5 μM.
Pengaruh Penambahan Gula Terhadap Waktu Fermentasi Sauerkraut Dari Kol (Brassica Oleracea L.) cynthia perdana putri; Resti Fevria; Moralita Chatri; Afifatul Achyar
Symbiotic: Journal of Biological Education and Science Vol. 1 No. 2 (2020)
Publisher : Fakultas Tarbiyah dan Ilmu Keguruan, Institut Agama Islam Negeri Kerinci

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32939/symbiotic.v1i2.7

Abstract

Cabbage (Brassica oleracea L) is a type of vegetable that is widely grown in high altitude areas, and has good benefits for health and can ward off disease. Cabbage is easily wilted, damaged and rotten. Fermentation is a food preservation process, which plays a role in improving the nutritional content, extending the shelf life of food. Fermentation of cabbage with the addition of salt is called sauerkraut. Fresh cabbage fermented into sauerkraut uses salt with a certain concentration, so no need to add other microorganisms as a starter (inoculum) or yeast, because lactic acid bacteria already exist in cabbage. This research is an experimental study carried out in December 2019 until January 2020, at the Biology Research Laboratory, FMIPA UNP. The design used in this study was a complete random design (CRD) with four treatments and five repetitions. In the treatment, 2.5% salt content and 2.5%, 5%, 7.5% and 10% sugar variation were used. The main observational parameters included fermentation time. Data were analyzed using the Analysis of Variance (ANOVA) test and further tested with DNMRT at the 0.05 level. Based on research results found that the addition of sugar in the fermentation process of sauerkraut affects the fermentation time.
Co-Authors Afionita, Santi Ahmad Hambali, Ahmad Ahmad Wibisana, Ahmad Alvenaya Hindayageni Ananda Putri, Ananda Annisa Irna Putri Annisa Khaira Annisa Khaira Annisa, Silvy Aprilia, Amanda Ara, Farrah Azzahra Ardi Ardi Atifah, Yusni Aura Zahra Nafisah Azizah, Jalilah Bintang Fadhil Ramadhan Cici Mustika cynthia perdana putri Dara Suci Amini, Dara Suci Des M Dezi Handayani Dezi Handayani Dina Sukma Dwi Hilda Putri Dwi Hilda Putri Dwi Hilda Putri Edwin Edwin Elsa Badriyya Elsa Yuniarti Elviana, Alifah Hazelia Fadhila Humaira Fardilla, Midratul Fatiha, Fathma Dwi Fevria, Resti Fitri, Afifah Ismu Fronica, Imelda Gilang Amanda Hafizah Fadhilah Hafizh Alza Afra Helsa Rahmatika Herisanti, Dini Irma Leilani Eka Putri Iyan Robiansyah Iyan Robiansyah Jalilah Azizah Jumatul Hafsah Kardiman, Reki Khairani, Fidia Aura Linda Advinda Marten, Threo Wanda Monica , Indiastri P Moralita Chatri Muhammad Farikh Muharani, Silvia Mukhlis Mukhlis Mutia Andini, Tri Nabilah, Rezi Nadira Nafisa Arini Nella Fauziah Novita, Yeni Nur Aqsha Nurfadillatun Nisa Wijaya Nurul Hasanah NURUL HIDAYAH Oliv Nurul Kanaya Nurul Kanaya Palupi, Indria Pinta, Sari Rahma Pradila, Andini Novalia Pramila, Cindy Pratama, Chelsylia Dara Pratama, Sandi Fransisco Pryatna, Muhamad Zacky Putri, Aulia Devani Putri, Irma Leilani Putri, Isna Aryunita Putri Putri, Santi Diana Putrizalda, Hafizhah Rahmad Wanizal Pastha Rahmawati, Atika Ayu Rahmi Holinesti Rezeki Rival Alridho Rezi Nabilah Ria Fernanda, Irma Rijal Satria Rijal Satria Rini Wulandari Rinti Mutiara Sari Riza Umami Roza, Sri Yenica Ruri Fitriyani Ruri Fitriyani S. Syamsurizal safitri, fira Salsabil, Velina Salsabilla, Vishtari Sari Rahma Pinta Sari, Rinti Mutiara Satria, Rijal Sefina, Nadia Selaras, Ganda Hijrah Sisca Alicia Farma Suryani, Elisa Vauzia Vauzia Vauzia, Vauzia Velina Salsabil Violita Violita Violita Violita Violita Violita Violita Yulita, Nelfi Yuni Ahda Yuni Ahda Zultsatunni’mah Zultsatunni’mah Zulyusri Zulyusri Zulyusri Zulyusri Zulyusri, Zulyusri Zulzusri, Zulzusri