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All Journal Bioscience Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Martabe : Jurnal Pengabdian Kepada Masyarakat Pelita Eksakta Jurnal Biogenerasi Journal of halal product and research (JHPR) 3BIO: Journal of Biological Science, Technology and Management Jurnal Jeumpa Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Promotor: Jurnal Mahasiswa Kesehatan Masyarakat Tropical Genetics Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi
Achyar, Afifatul
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang, Padang Indonesia
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Dermatoglyphic analysis of asthma sufferers in a population of students at the faculty of mathematics and science, Universitas Negeri Padang Salsabil, Velina; Achyar, Afifatul; Ahda, Yuni
Tropical Genetics Vol. 2 No. 2 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i2.30

Abstract

Asthma is a disease defined as chronic inflammation of the airways which is characterized by recurrent coughing and shortness of breath. Dermatoglyphs are the appearance of tendrils and tendril patterns on the fingers, palms, toes and soles. Dermatoglyph is used as an aid in the detection of a number of diseases that have a strong heritable basis and is also used to detect abnormalities. This study aims to analyze and compare the patterns of dermatoglyphics in asthma sufferers and non-asthmatics on the fingertips. This type of research is descriptive research with cross sectional sampling method. Samples were taken from the student population of the Faculty of Mathematics and Natural Sciences, Padang State University as many as 100 samples of fingerprint patterns. The results obtained showed that there was a significant difference in the number of arch fingerprint patterns of asthmatics compared to normal individuals. Seeing this significant difference in numbers indicates a possible link between fingerprint patterns and asthma phenotypes.
Analysis of VNTRs (variable number tandem repeat) D1S80 in Biology students class Sari Rahma Pinta; Pradila, Andini Novalia; Achyar, Afifatul
Tropical Genetics Vol. 3 No. 1 (2023):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v3i1.39

Abstract

DNA can be obtained from all parts of the body with the same profile in each person. The oral mucosal epithelium is one of the DNA sources that is often used for individual identification because of taking it using a swab method that does not hurt the volunteers. The purpose of this study was to determine DNA polymorphism in Biology students of Class of 2022. The sample used in this study were 49 people with details, 34 female and 15 male students. After cheek mucosa was sampling, the PCR and electrophoresis was performed to see the DNA bands formed. The visible band was measured in length and calculated using a formula to see the repeatability of the D1S80 locus on the DNA band. From the 49 samples which were collected, the DNA bands were seen in 27 samples with DNA band lengths ranging from 400-600 bp. The number of bands in each sample were also different. The highest frequency of locus repetitions was 26 repetitions and the lowest was 14 repetitions.
Dermatoglyphic analysis of left-handed individuals in the student population of the Faculty of Mathematics and Natural Sciences Universitas Negeri Padang Herisanti, Dini; Achyar, Afifatul; Ahda, Yuni
Tropical Genetics Vol. 3 No. 1 (2023):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v3i1.41

Abstract

Dermatoglyphics is a science that studies fingerprint patterns that can be inherited genetically. Fingerprint patterns can be divided into three patterns, arch, loop, and whorl. Observation of fingerprints is used to determine fingerprint patterns and the relationship between these patterns and the fields of health, education, and criminology. Left-handed is a term for people who predominantly use their left hand as their main hand to carry out daily activities. This research aims to determine the relationship between fingerprint patterns and individuals who tend to carry out activities with the left side of their body (left-handed). The type of research carried out was descriptive research with a cross-sectional sample collection method. The sample was taken from the 2019-2022 student population of the Faculty of Mathematics and Natural Sciences, Padang State University, totaling 100 individuals. The samples were taken in November and December. This research was carried out at the Genetics and Biotechnology Laboratory, Faculty of Mathematics and Natural Sciences, Padang State University. The results of this study show that the dominant fingerprint pattern found in the left-handed individual phenotype is 16 fingerprint patterns, while normal individuals have 5 arch fingerprint patterns. This shows that there is a link between the phenotype of left-handed individuals and dermatoglyphics.
Analysis of genetic sequence variation in Formicidae NCBI popset 1871743971 using in silico RFLP Pryatna, Muhamad Zacky; Satria, Rijal; Achyar, Afifatul
Tropical Genetics Vol. 4 No. 1 (2024): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v4i1.43

Abstract

In practically every region of the world, with the exception of the poles, ants are a common form of insect. This one organism lives in colonies and cooperates, therefore these insects are referred to as social insects. In an ant colony, three types of castes have their respective roles and functions, namely, there are queen ants, worker ants, and male ants. In one ant species, many colonies live scattered and each of them has different genes that are influenced by environmental factors and other factors. The purpose of this study was to determine and analyze the genetic variation of ants with the WNT-1 gene sequence in NCBI popset 1871743971. This research was conducted in May-June 2023 using the RFLP method in silico. Based on the results of the study, it was found that there were genetic variations in the WNT-1 gene sequence in Formicidae available at NCBI in PopSet 1871743971.
Analysis of genetics variation of the ndhF gene sequence in Antrophyum sp. NCBI popset 2496377569 using in silico RFLP Hafizah Fadhilah; Afifatul Achyar
Tropical Genetics Vol. 3 No. 2 (2023): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v3i2.44

Abstract

Antrophyum is one type of the largest and most diverse of the vittarioid ferns (Pteridaceae) in tropical Asia and the Pacific Islands. The data found in the Antrophyum study is still relatively sparse because the diversity of the genus is still uncertain. ndhF gene is an inherited gene from chloroplast DNA (NADH dehydrogenase five). Chloroplast DNA controls the production of ribosomal RNA (rRNA), transfer RNA (tRNA), and most of the proteins contained in the organelles chloroplast. Genes are commonly used in molecular studies such as the analysis of genetic variation in plants.. Genetic variation indicates that the greater the genetic variation that occurs in a population, the better its ability to adapt to the environment.. Besides that, determining genetic variation can also prevent extinction of a plant. This study aims to analyze genetic variation in the ndhF gene sequence of Antrophyum sp. NCBI PopSet 2496377569. In this study, the RFLP molecular marker was used which was carried out in silico using two restriction enzymes, PciI and ScaI.. The results of this study showed that there was no genetic variation in digestion with the restriction enzyme PciI, whereas in ScaI there was genetic variation with 3 allele variations from 23 PopSet sequences 2496377569 in NCBI.
Analysis of rbcL gene sequence genetic variation in Dermatophyllum gypsophilum subsp. guadalupense NCBI Popset 2440747772 using RFLP in silico Cici Mustika; Achyar, Afifatul
Tropical Genetics Vol. 3 No. 2 (2023): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v3i2.47

Abstract

Dermatophyllum gypsophilum subsp. guadalupense is a rare species of flowering plant in the legume family known by the common name Guadalupe Mountain necklacepod. This plant is native to New Mexico and Texas in the United States. The rbcL gene encoding the RuBisCO protein is thought to cause this gene sequence to have a low mutation rate compared to other barcode genes in cpDNA so that the level of similarity between species is quite high. This study used restriction enzyme PciI. This study aimed to analyze genetic variation in rbcL gene sequence in Dermatophyllum gypsophilum subsp. guadalupense NCBI with PopSet 2440747772 using RFLP in silico. The results of this study showed genetic variations in the rbcL gene sequence of Dermatophyllum gypsophilum subsp. guadalupense and 8 allele variations contained in 73 gene sequences using the restriction enzyme PciI.
Analysis of Genetic Variation of MatK Gene Sequences in Ammothamnus lehmannii NCBI Popset 2440747918 Using In Silico RFLP Amini, Dara Suci; Afifatul Achyar
Tropical Genetics Vol. 3 No. 2 (2023): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v3i2.49

Abstract

Genetic diversity or genetic variation is variation that occurs in an organism due to differences in the sequence of nucleotide bases (adenine, thymine, guanine and cytosine) that form DNA in cells. Variation genetics can be studied in silico using available gene sequences in the NCBI genbank database. This study used the MatK (Maturase-K) gene sequence with the identity number Popset 2440747918 which was downloaded in fasta format from NCBI . Then screening of restriction enzyme candidates was carried out to determine the restriction enzymes prior to in silico RFLP. The restriction enzyme selected from the screening was the restriction enzyme HindIII which has the recognition site A'AGC_T. The results obtained from 79 samples of DNA sequences, 76 samples were cut and 3 samples were not. And found three allele variations with the percentage of the presence of fragments A1 (86.07%), A2 (10.12%) and A3 (3.79%). The percentage values and frequencies of these A1, A2 and A3 alleles indicate a low level of genetic variation.
Analysis of Genetic Variations in bHLH Protein (Rc) Gene Sequences in Rice (Oryza) NCBI PopSet 2496581476 Using In-Silico RFLP: Analisis Variasi Genetik Sekuen Gen Rc-bHLH Protein pada Padi (Oryza) NCBI PopSet 24596581476 Menggunakan RFLP secara In-Silico Hasanah, Nurul; Violita, Violita; Achyar, Afifatul
Tropical Genetics Vol. 3 No. 2 (2023): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v3i2.50

Abstract

Rice is a plant from the Graminae Family that is spread almost throughout the region. In rice, there is an Rc gene that codes for the formation of the basic loop-helix-loop (bHLH) protein, the Rc gene (Rc-bHLH) functions as a transcription of color pigments in brown rice. This research was conducted computationally from several related sites. The study used restriction enzyme BstUI with a side recognition of 5'-CG'CG-3'. The purpose of this study was to analyze the genetic variation of the Rc gene sequence encoding the bHLH protein in NCBI PopSet rice 2496581476 using RFLP in silico. The results showed that there were genetic variations of the Rc-bHLH gene, 2 allele variations were present in 21 rice sequences using the restriction enzyme BstUI.
Analysis of genetic variation of the voltage-gated sodium chanel gene sequence in Aedes aegypti NCBI popset (2325355280) using in silico RFLP Putrizalda, Hafizhah; Achyar, Afifatul
Tropical Genetics Vol. 4 No. 1 (2024): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v4i1.52

Abstract

Aedes aegypti is a mosquito that belongs to the Culicinae Culicids family, order Diptera, class Insecta. This mosquito has the potential to transmit dengue hemorrhagic fever (DHF). DHF is a disease characterized by sudden fever, bleeding both in the skin and in the body and can cause shock and death. In-silico is a research approach whose use will be discussed with advances in technology and available databases. This approach is very commonly used in medical and other healthcare settings. The method used is to use the NCBI site, screen restriction enzymes with an insilico web and continue with the benchling site. The results obtained were using the NheI enzyme and producing two alleles namely Allele A1 and Allele A2. The A2 allele dominates because it has a fragment percentage of 63,3%.
Isolation and Identification of Lactic Acid Bacteria Using PCR Gene from Tempe Wrapped with Banana Leaves and Plastic Fevria, Resti; Vauzia, Vauzia; Putri, Dwi Hilda; Achyar, Afifatul; Putri, Santi Diana; Edwin, Edwin
Indonesian Food Science and Technology Journal Vol. 7 No. 2 (2024): Volume 7. Number 2, July 2024 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v7i2.32503

Abstract

Tempe is a typical Indonesian food that comes from fermenting soybeans with the fungus Rhizopus sp. Tempe is known to have good nutritional value because of the content contained in soybeans themselves and other microorganisms that appear as a result of the tempe fermentation process. The fermentation process increases the activity of bacteria in tempe which are beneficial for digestion, one of which is lactic acid bacteria. This research aims to see the differences morphological forms  and genomic of lactic acid bacteria produced by tempe wrapped in banana leaves and tempe wrapped in plastic. The differences in fermentation that occurred in tempe wrapped in banana leaves and those wrapped in plastic resulted in differences in lactic acid bacteria type. Based on the isolation of bacteria on Mann de Rogosa Sharpe Agar medium, 15 isolates of lactic acid bacteria were produced, with general morphological forms of bacilli and coccus. Then, genomic identification was carried out using the PCR. The phylogenetic tree built based on the 16S rRNA gene sequence can show the relationship between LAB diversity at the species level, but cannot differentiate LAB from the strain level.
Co-Authors Afionita, Santi Ahmad Hambali, Ahmad Ahmad Wibisana, Ahmad Alvenaya Hindayageni Ananda Putri, Ananda Annisa Irna Putri Annisa Khaira Annisa Khaira Annisa, Silvy Ara, Farrah Azzahra Ardi Ardi Atifah, Yusni Aura Zahra Nafisah Bintang Fadhil Ramadhan Cici Mustika cynthia perdana putri Dara Suci Amini, Dara Suci Des M Dezi Handayani Dwi Hilda Putri Dwi Hilda Putri Dwi Hilda Putri Edwin Edwin Edwin Edwin Elsa Badriyya Elsa Yuniarti Elviana, Alifah Hazelia Fadhila Humaira Fatiha, Fathma Dwi Fevria, Resti Fitri, Afifah Ismu Gilang Amanda Hafizah Fadhilah Hafizh Alza Afra Helsa Rahmatika Herisanti, Dini Indiastri P Monica Irma Leilani Eka Putri Iyan Robiansyah Iyan Robiansyah Jalilah Azizah Jumatul Hafsah Khairani, Fidia Aura Linda Advinda Marten, Threo Wanda Moralita Chatri Muhammad Farikh Muharani, Silvia Mutia Andini, Tri Nadira Nafisa Arini Nella Fauziah Nur Aqsha Nurfadillatun Nisa Wijaya Nurul Hasanah NURUL HIDAYAH Oliv Nurul Kanaya Nurul Kanaya Pinta, Sari Rahma Pradila, Andini Novalia Pramila, Cindy Pratama, Chelsylia Dara Pryatna, Muhamad Zacky Putri, Isna Aryunita Putri Putri, Santi Diana Putrizalda, Hafizhah Rahmad Wanizal Pastha Rahmawati, Atika Ayu Rezeki Rival Alridho Rezi Nabilah Rijal Satria Rijal Satria Rini Wulandari Rinti Mutiara Sari Ruri Fitriyani Ruri Fitriyani S. Syamsurizal safitri, fira Salsabil, Velina Salsabilla, Vishtari Sari Rahma Pinta Sari, Rinti Mutiara Satria, Rijal Sefina, Nadia Sisca Alicia Farma Suryani, Elisa Vauzia Vauzia Vauzia, Vauzia Vauzia, Vauzia Velina Salsabil Violita Violita Violita Violita Violita Violita Violita Yulita, Nelfi Yuni Ahda Yuni Ahda Zultsatunni’mah Zultsatunni’mah Zulyusri Zulyusri Zulyusri Zulyusri Zulyusri, Zulyusri Zulzusri, Zulzusri