Articles
<![CDATA[Optimasi Deteksi Kontaminasi Daging Babi Berbasis Multiplex Real Time Polymerase Chain Reaction (qPCR) pada Produk Makanan Olahan Daging Sapi ]]>
<![CDATA[Pramila, Cindy]]>;
<![CDATA[Achyar, Afifatul]]>
<![CDATA[ Jurnal Serambi Biologi Vol. 8 No. 4 (2023): Jurnal Serambi Biologi ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang ]]>
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DOI: 10.24036/srmb.v8i4.239
<![CDATA[Abstrak. Indonesia merupakan salah satu negara dengan mayoritas penduduk beragama Islam. Mengonsumsi pangan yang halal adalah hak dasar setiap muslim. Sampai saat ini harga daging sapi masih relatif mahal. Konsekuensi dari mahalnya harga daging tersebut yaitu ada oknum yang mencampur atau mengganti daging sapi dengan daging hewan lain seperti daging babi. Produk pangan yang dibuat dengan campuran daging sangat sulit dibedakan dengan mata telanjang. Metode Multiplex Real Time PCR merupakan metode pengujian yang cepat, sensitif, dan spesifik untuk mendeteksi kontaminasi daging babi pada produk pangan olahan daging sapi. Hasil penelitian menunjukkan konsentrasi primer yang optimum untuk pasangan primer Bos dan Sus adalah 0,4 μM. Konsentrasi DNA yang optimum untuk mengamplifikasi DNA Bos taurus dan Sus scrofa yaitu pada konsentrasi 100 ng/ μl yang menghasilkan amplikon dengan nilai peak 86,3 o C untuk Bos taurus dan 84,7 oCpada Sus scrofa. Kata kunci optimasi Multiplex Real Time PCR, gen ND5, babi, daging sapi, halal]]>
<![CDATA[Optimasi Isolasi DNA Bakteri Patogen pada Sampel Air Sungai Berbasis PCR ]]>
<![CDATA[Putri, Ananda]]>;
<![CDATA[Achyar, Afifatul]]>
<![CDATA[ Jurnal Serambi Biologi Vol. 8 No. 4 (2023): Jurnal Serambi Biologi ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang ]]>
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DOI: 10.24036/srmb.v8i4.241
<![CDATA[Abstrak Bakteri patogen adalah mikroorganisme yang menyebabkan penyakit pada inangnya dengan berbagai proses yang secara jelas melalui kerusakan langsung jaringan atau sel selama replikasi, melalui produksi toksin yang memungkinkan patogen mencapai jaringan baru atau keluar dari sel pada tempat bereplikasi. Bakteri patogen seperti E.coli dapat tersebar melalui air sungai karena sungai banyak dijadikan sebagai tempat pembuangan kotoran dan sampah sehingga menjadi penyebaran bakteri patogen. Penelitian ini bertujuan untuk mengetahui optimasi isolasi DNA dan mendeteksi bakteri patogen pada sampel air sungai. Metodologi yang digunakan dalam penelitian ini adalah pengambilan sampel air sungai gangga di sekitar Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Padang dan dilakukan analisis laboratorium. Hasil penelitian dari optimasi sampel air sungai gangga bahwa primer yang optimal untuk mendeteksi bakteri patogen adalah primer ESS. Primer ESS berpotensi mengamplikasi bakteri E.coli, Salmonella, dan, Shigella dengan ukuran amplikon 825 bp. Optimasi isolasi lebih optimal saat sampel dimasukkan kedalam microtube .]]>
<![CDATA[Genetic Variation Analysis of the E6 HPV 16 Gene Using RFLP In Silico ]]>
<![CDATA[Annisa, Silvy]]>;
<![CDATA[Rahmawati, Atika Ayu]]>;
<![CDATA[Nadira]]>;
<![CDATA[Khairani, Fidia Aura]]>;
<![CDATA[Achyar, Afifatul]]>
<![CDATA[ Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang ]]>
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DOI: 10.24036/srmb.v9i1.331
<![CDATA[Cervical cancer is cancer that attacks epithelial cells or the outer surface layer of the cervix. This cancer is most commonly caused by the high risk type of Human Papillomavirus. The RFLP method uses restriction enzymes to cut certain nucleotide sequences at specific positions that produce fragments of different lengths. The purpose of this study was to determine the polymorphism that occurs in the E6 gene of the HPV 16 virus. This research was conducted using RFLP in silico. Virtual descriptive methods are used to analyze data and collect information about the object of study. The restriction enzyme HpaII was used in this study. The results showed that there were two alleles (A1 and A2) out of a total of 15 sequences of the E6 HPV 16 gene in Popset 636528409 indicating that there was a genetic variation in the gene.]]>
<![CDATA[Gambaran Kadar Hemoglobin (Hb) pada Ibu Hamil dengan Menggunakan Alat Hematologi Analyzer di Puskesmas Lubuk Basung ]]>
<![CDATA[Fitri, Afifah Ismu]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Yuniarti, Elsa]]>
<![CDATA[ MASALIQ Vol 4 No 4 (2024): JULI ]]>
Publisher : <![CDATA[Lembaga Yasin AlSys ]]>
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DOI: 10.58578/masaliq.v4i4.3341
<![CDATA[Hematological changes in pregnant women are a response to multifactorial hormonal changes, one of the hematological changes that occur is caused by anemia. Anemia in pregnant women is physiological that can occur in normal pregnancy. Some factors that can be experienced by pregnant women to experience anemia are nutritional knowledge factors, age factors that are too young or too old, and health in the nutritional consumption patterns of pregnant women. The purpose of this study was to see a description of hemoglobin (hb) levels in pregnant women using a hematology analyzer at the Lubuk Basung health center. The implementation of activities carried out at the Lubuk Basung Health Center Health Service Laboratory, Agam Regency, West Sumatra Province by performing venous blood collection procedures and then performing hemoglobin level examination procedures using a hematology analyzer. the conclusions obtained are the results of research conducted on 69 samples in June-July 2023 on the description of hemoglobin levels in pregnant women at the Lubuk Basung Health Center, it was concluded that of the 69 samples there were pregnant women with an age range of 20-35 years totaling 62 people (89.9%), and age> 35 years totaling 7 people (10.1%). This shows that pregnant women who do Hb checks at the Lubuk Basung Health Center tend to be productive age, and of the 69 samples, low Hb levels (<11 gr/dl) were 17 people (24.6%) and normal Hb levels (11-15 gr/dl) were 52 people (75.4%).]]>
<![CDATA[Optimization of Deoxyribonucleic Acid (DNA) isolation methods from several types of cosmetic samples for molecular-based halal tests ]]>
<![CDATA[Nafisa Arini]]>;
<![CDATA[Afifatul Achyar]]>
<![CDATA[ Journal of Halal Product and Research (JHPR) Vol. 6 No. 1 (2023): The Development of Global Halal: Issues and Challenges ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
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DOI: 10.20473/jhpr.vol.6-issue.1.1-10
<![CDATA[Bagi seorang muslim status halal suatu produk pangan maupun non-pangan harus terpenuhi secara mutlak, begitu juga dengan produk kosmetik. Kosmetik terdiri dari berbagai bahan dan yang menjadi titik kritis status kehalalan suatu produk kosmetik adalah kompleksitas komposisi produk kosmetik yang memungkinkan penggunaan bahan dasar yang berasal dari babi seperti gelatin, asam lemak, gliserin dan kolagen yang mana bahan-bahan tersebut adalah bahan yang sangat umum digunakan dalam pembuatan produk kosmetik. Tujuan dilakukannya penelitian ini adalah untuk untuk mengoptimasi metode isolasi DNA pada beberapa jenis sampel kosmetik untuk uji halal berbasis molekuler. Penelitian dilakukan dalam tiga tahap, yaitu ekstraksi DNA, amplifikasi dan analisis rt-PCR, dan tahap terakhir adalah visualisasi dengan media elektroforesis gel agarose. Optimasi isolasi DNA dilakukan dengan tiga metode yang berbeda, yaitu dengan menggunakan kit isolasi DNA QIAamp® DNA Mini Kit (QIAGEN), isolasi dengan laruta phenol-chlororoform GENEzolâ„¢ (Geneaid), dan menggunakan campuran resin Chelex-TE 10%. Hasil menunjukkan bahwa metode isolasi terbaik dari ketiga metode yang dilakukan adalah metode isolasi DNA dengan menggunakan QIAamp® DNA Mini Kit (QIAGEN) yang mana berdasarkan visualisasi elektroforesis metode ini dapat mengisolasi DNA dari semua sampel.]]>
<![CDATA[Genetic Variation Analysis of L1 gene HPV-16 using RFLP in Silico ]]>
<![CDATA[Afionita, Santi]]>;
<![CDATA[Putri, Dwi Hilda]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Yuniarti, Elsa]]>
<![CDATA[ Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni ]]>
Publisher : <![CDATA[Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia ]]>
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DOI: 10.29303/jbt.v25i2.8415
<![CDATA[Human papillomavirus (HPV) is one of the leading causes of cervical cancer, with the L1 gene playing an important role in vaccine development. This study aims to analyze the genetic variation of the L1 gene of HPV types 16, 18, 52, and 58 using the RFLP method in silico by utilizing restriction enzymes. The results showed that. The pattern of genetic conservation is more dominant in HPV 16 and 52 than other types, indicating the potential important role of the L1 gene in vaccine development. This study confirms the importance of analyzing genetic variation to understand HPV genetic diversity. These results may contribute to the development of more specific vaccines and molecular markers for HPV detection.]]>
<![CDATA[ANALISIS POHON FILOGENETIK GEN ITS1 Amanita muscaria DARI BERBAGAI NEGARA ]]>
<![CDATA[Mutia Andini, Tri]]>;
<![CDATA[Achyar, Afifatul]]>
<![CDATA[ Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues) ]]>
Publisher : <![CDATA[Universitas Cokroaminoto Palopo ]]>
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DOI: 10.30605/biogenerasi.v10i2.5799
<![CDATA[The purpose of this study is to determine the genetic diversity of the ITS1 gene from Amanita muscaria originating from various countries and create a phylogenetic tree based on ITS1 gene data to show the evolutionary relationship and distribution history of Amanita muscaria in various countries.The method used in this study is the Neighbor-Joining Method which is used to construct phylogenetic trees quickly and efficiently. Bootstrap tests (10,000 replicates) indicate the confidence level of the tree branches. Evolution was calculated using the Maximum Composite Likelihood method. The analysis involved 13 nucleotide sequences and was performed with MEGA11 software. The results of this study are The phylogenetic tree of Amanita muscaria shows two main clades with bootstrap values of 27 and 39, which reflect the level of confidence in the group of specimens. The first clade with a value of 27 includes specimens AB096048.1, AJ549964.1, AB080778.1, AB081295.1, and LN877747.1, while the second clade with a value of 39 includes AB015700.1, AB081294.1, AB080779.1, and AB080983.1. The branch with a bootstrap value of 63 shows greater genetic diversity. Specimens AB096048.1 and AJ549964.1 and AB080777.1 and AB096052.1 have very close evolutionary relationships.]]>
<![CDATA[DESAIN PRIMER GEN EDNRB EKSON 4 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG DISEASE ]]>
<![CDATA[Hidayah, Nurul]]>;
<![CDATA[Ahda, Yuni]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Atifah, Yusni]]>
<![CDATA[ Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues) ]]>
Publisher : <![CDATA[Universitas Cokroaminoto Palopo ]]>
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DOI: 10.30605/biogenerasi.v10i2.5954
<![CDATA[EDNRB gene is a gene located on chromosome 13q22, plays a role in the signaling pathway during the development of the ENS intestinal nerves. Mutations in the EDNRB gene in humans are associated with Hirschsprung Disease (HD). Mutations in the EDNRB gene are the second most common cause after the RET gene in cases of HD with a percentage of 5–10%. To detect mutations, PCR products are needed. Primers are one of the basic components of PCR, while optimization of annealing temperature and optimization of primer concentration are factors in the success of primer attachment to the target gene. This study aims to design EDNRB exon 4 gene primers and determine the optimum annealing temperature and primer concentration to amplify the EDNRB gene. Primer design with Geneious Prime, and good primer criteria are analyzed in silico. Optimization of annealing temperature and primer concentration is carried out with gradient PCR and variations in primer concentration. The results of this study obtained the best primers, namely the forward primer 5'- CAGTAAGTGTGGCCTGAAAG-3' and the reverse primer 5'-GTGGAACCGAAGTGACTAGA-3', which amplified the EDNRB gene exon 4. The optimum PCR conditions for amplification of exon 4 of the EDNRB gene were at an annealing temperature of 57.2 ℃ and at a primer concentration of 0.5 μM.]]>
<![CDATA[Optimisation of 25-OH D Elisa Kit Usage Using EDTA, Heparin, and Gel Blood Tubes ]]>
<![CDATA[Sefina, Nadia]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Wahyuni, Ira]]>
<![CDATA[ PROMOTOR Vol. 8 No. 5 (2025): OKTOBER ]]>
Publisher : <![CDATA[Universitas Ibn Khaldun Bogor ]]>
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DOI: 10.32832/pro.v8i5.1388
<![CDATA[The use of anticoagulants in blood collection tubes is also an important factor in the measurement of vitamin D and its metabolites. Vitamin D levels in circulating blood can be measured by measuring 25-hydroxyvitamin D levels in blood. This study aims to compare the effectiveness of using EDTA, heparin, and gel blood tubes in maintaining the stability of 25-OH D in blood samples. The type of research used is Analytical Descriptive research. The samples used in this study used venous blood which was determined using the Simple Random Sampling technique. Samples were taken from 5 semester S1 internship students totalling three people to take blood. Where the blood was taken as much as 3 cc and divided into several purple, green and yellow vacuntainer tubes. From the data obtained, it shows that the use of vacutainer tubes in blood storage to obtain serum for the most optimum vitamin D level test is in a tube with a green lid containing lithium heparin anticoagulant. Where the results obtained on lithium heparin anticoagulant are in the range of >60 ng/mL. As strong evidence of no potential adverse effects at high (optimal) levels. Tubes with purple or lavender coloured covers containing EDTA had results <20 ng/mL indicating that vitamin D deficiency may occur due to lack of vitamin D nutrition. On the other hand, the tube with yellow coloured lid containing anticoagulant gel showed >100 ng/mL indicating vitamin D excess.]]>
<![CDATA[Dextran Producing Bacteria: A Literatur Review ]]>
<![CDATA[Salsabilla, Vishtari]]>;
<![CDATA[Putri, Dwi Hilda]]>;
<![CDATA[Advinda, Linda]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Wibisana, Ahmad]]>
<![CDATA[ Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret ]]>
Publisher : <![CDATA[Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia ]]>
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DOI: 10.29303/jbt.v25i1.8370
<![CDATA[This study aims to analyze dextran-producing bacteria, especially from the general Leuconostoc, Weissella, and Lactobacillus, which produce the enzyme dextransucrase for dextran synthesis. This research uses the Systematic Literature Review (SLR) method based on the PRISMA protocol, with data sources from various trusted scientific databases for the 2020–2024 period. The results of the study show that variations in dextran structure, such as molecular weight and degree of branching, are highly dependent on the bacterial strain and fermentation conditions. Optimization of production parameters, including pH, temperature and substrate concentration, can increase dextran production efficiency The conclusions of this study emphasize the need for a multidisciplinary approach to optimize the potential of dextran in industrial and biotechnology applications. Further research is recommended to explore new strains, optimize production processes, and diversify dextran applications.]]>
