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All Journal JURNAL KIMIA SAINS DAN APLIKASI Bioscience AL KAUNIYAH Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Martabe : Jurnal Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN TAMBUSAI Pelita Eksakta Jurnal Biogenerasi Journal of halal product and research (JHPR) 3BIO: Journal of Biological Science, Technology and Management Jurnal Jeumpa Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Promotor: Jurnal Mahasiswa Kesehatan Masyarakat Tropical Genetics Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi
Achyar, Afifatul
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang, Padang Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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The Effect of miR-10b Mimic Transfection on Invasion and Proliferation Gene Expression as the Direct Target of the miR-10b Pathway in T47D Breast Cancer Cell Lines Arisanty, Dessy; Eshak, Zolkapli; Wahyuni, Fatma Sri; Achyar, Afifatul; Khairani, Salsabila Putri
Jurnal Kimia Sains dan Aplikasi Vol 29, No 1 (2026): Volume 29 Issue 1 Year 2026
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.29.1.58-63

Abstract

Breast cancer remains one of the leading causes of death in women worldwide, with high incidence and mortality rates. The abnormal expression of the small non-coding RNA, microRNA-10b (miR-10b), has been associated with breast cancer progression. miR-10b is involved in various biological processes, including proliferation, metastasis, and drug resistance. However, the underlying mechanism by which miR-10b contributes to breast cancer progression remains unclear. This study aimed to investigate the effect of miR-10b on the expression of HOXD10 and PI3K genes, as well as its impact on cell viability and proliferation in breast cancer cells. In this study, T47D breast cancer cells were transfected with mimic miR-10b using the FANA-oligo transfection method at concentrations of 1, 2.5, and 5 µM. Cell viability was assessed using the MTT assay, while HOXD10 and PI3K gene expression levels were measured using RT-qPCR and ELISA. Data were analysed using paired t-tests with SPSS, and results were considered statistically significant at p < 0.05. The results showed that mimic miR-10b induced a dose-dependent increase in cell proliferation. Notably, only HOXD10 gene expression was significantly affected by the transfection, while PI3K gene expression remained unchanged. Cells transfected with 5 µM miR-10b mimic exhibited the highest proliferation rate compared to non-transfected controls (p < 0.05). In conclusion, miR-10b promotes breast cancer cell proliferation by downregulating HOXD10 expression, independent of PI3K, suggesting it is a potential target for further investigation.
EDUKASI JAJANAN SEHAT KEPADA SISWA SD DALAM MENGHADAPI ERA NEW NORMAL Yusni Atifah; Afifatul Achyar; Rijal Satria; Violita Violita; Helsa Rahmatika; Jalilah Azizah
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 6, No 1 (2023): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v6i1.213-217

Abstract

Anak usia sekolah memerlukan asupan energi dan zat gizi untuk mendukung pertumbuhan dan juga prestasi belajar anak di sekolah. Salah satu sumber asupan energi dan zat gizi bagi anak sekolah bersumber dari pangan jajanan. Anak usia sekolah sebagai generasi penerus bangsa merupakan investasi bagi suatu bangsa. Kualitas anak-anak pada masa sekarang akan menentukan kualitas suatu negara di masa depan. Edukasi keamanan pangan jajanan perlu dilakukan sejak dini secara sistematis dan juga berkelanjutan sebagai salah satu upaya untuk peningkatan kualitas sumber daya manusia. Pemberian nutrisi yang berkualitas, sehat dan sesuai dengan kuantitas yang seharusnya penting untuk menunjang pertumbuhan perkembangan anak usia sekolah agar optimal. Oleh karena itu, perlu adanya edukasi kepada siswa SD untuk meningkatkan pengetahuan terkait keamanan pangan jajanan dan mengetahui arti simbol-simbol pada bungkus jajanan. Kegiatan ini dilaksanakan pada bulan Juli 2022, dan diikuti oleh 206 peserta dari kelas 3 dan 6 SDN 02 Payakumbuh. Hasil evaluasi kegiatan menunjukkan adanya peningkatan pengetahuan siswa terhadap keamananan jajanan dan memiliki keterampilan dalam mengidentifikasi simbol-simbol pada bungkus jajanan. Kesimpulan untuk kegiatan ini sudah efektif dilaksanakan dan dapat mengedukasi siswa SD dalam  mengetahui keamanan pangan jajanan.
Analisis Pohon Filogenetik Genus Oryza Berdasarkan Gen matK Marizky Siregar, Yoana; Violita, Violita; Achyar, Afifatul
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/6myncp72

Abstract

The genus Oryza includes both cultivated and wild rice species that play an important role in global food systems and crop improvement. These species also exhibit complex evolutionary patterns, making the study of their phylogenetic relationships essential for understanding domestication and genetic diversity. In this study, phylogenetic relationships within the genus Oryza were analyzed using chloroplast matK gene sequences through a bioinformatics approach. A total of twelve species, representing both cultivated and wild taxa, were selected from the GenBank database. Sequence alignment and tree reconstruction were carried out using MEGA version 11. The phylogenetic tree was generated using the Neighbor-Joining method with the Maximum Composite Likelihood model, and its reliability was assessed through 10,000 bootstrap replications. The results show a clear distinction between cultivated and wild Oryza species. Oryza sativa (Japonica and Indica) is closely associated with O. nivara, supported by high bootstrap values, indicating a strong evolutionary link. Meanwhile, several wild species form separate clades, suggesting earlier divergence within the genus. Overall, the matK gene proves useful for describing phylogenetic relationships at the genus level, although its ability to resolve relationships among closely related species remains limited. These findings contribute to a better understanding of the evolutionary structure of Oryza and highlight the potential importance of wild rice species in future conservation and breeding efforts.
ANALISIS FILOGENETIK GEN atpA PADA GENUS Oryza MENGGUNAKAN PERANGKAT LUNAK MEGA DENGAN PENDEKATAN NEIGHBOR-JOINING Rafila, Zacki; Violita; Achyar, Afifatul
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/1ptac474

Abstract

This study aims to analyze the phylogenetic relationship of the atpA gene in the genus Oryza using Molecular Evolutionary Genetics Analysis (MEGA) software. The atpA gene, which plays a role in ATP synthesis in chloroplasts, was selected because of its relatively conserved nature and relevance in plant molecular evolution studies. The atpA gene sequences from 13 Oryza species were obtained from the NCBI GenBank database and analyzed using the Neighbor-Joining (NJ) method with the Kimura 2-Parameter model to reconstruct the phylogenetic tree. The reliability of the tree branches was tested through a bootstrap analysis of 10,000 repetitions. The results showed a pattern of kinship consistent with existing taxonomic classifications, in which Oryza sativa tended to form a single clade with Oryza rufipogon as its closest ancestor, while wild species such as Oryza punctata and Oryza officinalis formed a separate and more divergent clade. The relatively high bootstrap values at several branches indicate the reliability of the phylogenetic inferences generated. These findings reinforce the hypothesis that rice domestication involved wild species genetically close to cultivated species and suggest that the atpA gene has the potential to be used as an effective molecular marker in phylogenetic studies of the genus Oryza.
Analisis Filogenetik Bakteri Escherichia coli Berdasarkan DNA Genomik Gen BlaTEM Dari Data Sekuens NCBI: Pendekatan Bioinformatika terhadap Variasi Gen BlaTEM pada Escherichia coli Tahun 2016–2025 Indria Palupi; Afifatul Achyar
Tropical Genetics Vol. 5 No. 2 (2025): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v5i2.102

Abstract

Resistensi antibiotik yang disebabkan oleh bakteri penghasil β-laktamase, seperti Escherichia coli, menjadi permasalahan global yang serius. Gen blaTEM merupakan salah satu gen utama yang berkontribusi terhadap resistensi tersebut. Penelitian ini bertujuan untuk menganalisis hubungan filogenetik antar isolat Escherichia coli berdasarkan sekuens DNA gen blaTEM yang diambil dari database NCBI dalam rentang waktu 2016 hingga 2025. Data sekuens diselaraskan menggunakan metode ClustalW dalam perangkat lunak MEGA X, kemudian dianalisis menggunakan metode Neighbor-Joining dan 1000 bootstrap. Hasil analisis menunjukkan pembentukan beberapa klade utama dengan tingkat kedekatan genetik yang bervariasi. Kelompok isolat tahun 2021–2024 memiliki kedekatan genetik tinggi, sedangkan isolat tahun 2020 dan 2025 menunjukkan divergensi yang signifikan. Temuan ini mengindikasikan adanya variasi evolusioner dalam gen blaTEM yang penting untuk dipantau sebagai bagian dari strategi pengendalian resistensi antibiotik.
Pengaruh Penambahan Cabai ( Capsicum annum ) Dan Gula Terhadap Total Bakteri Asam Laktat (BAL) Dari Sauerkraut Vauzia Vauzia; Resti Fevria; Indiastri P Monica; Moralita Chatri; Afifatul Achyar; Edwin Edwin
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.4811

Abstract

Sauerkraut is a typical German food made from thinly sliced cabbage. Sauerkraut is a fermeted product that can give a distinctive taste. Sugar is an ingredient that can be added in, that sugar can trigger the growth of lactic acid bacteria. Indonesian people like food with a spicy sensation from chilies, chillies here contain vitamin C which functions as an antioxidant that is good for the body and is able to increase endurance. This study aims to calculate the total lactic acid bacteria contained in sauerkraut with the addition of chilies and sugar. Dilution of 1 ml sample into 9 ml physiological NaCl solution from 10 -1 to 10 -8 dilutions was carried out, then inoculated into MRS Agar using the pour plate method. Incubated in room temperature for 48 hours. The growing colonies of lactic acid bacteria were then observed based on colony morphology and counting the total growing lactic acid bacteria. The results of the research can be concluded that there was an increase in the number of lactic acid bacteria in sauerkraut with the addition of chili and 10% granulated sugar with a bacterial density average is 88.667 x 108 CFU/mL.
Co-Authors Afionita, Santi Ahmad Hambali, Ahmad Ahmad Wibisana, Ahmad Alvenaya Hindayageni Ananda Putri, Ananda Annisa Irna Putri Annisa Khaira Annisa Khaira Aprilia, Amanda Ara, Farrah Azzahra Ardi Ardi Atifah, Yusni Aura Zahra Nafisah Bintang Fadhil Ramadhan Cici Mustika cynthia perdana putri Dara Suci Amini, Dara Suci Des M Dessy Arisanty Dezi Handayani Dezi Handayani Dina Sukma Dwi Hilda Putri Dwi Hilda Putri Dwi Hilda Putri Edwin Edwin Edwin Edwin Elsa Badriyya Elsa Yuniarti Elviana, Alifah Hazelia Fadhila Humaira Fardilla, Midratul Fatiha, Fathma Dwi Fatma Sri Wahyuni Fevria, Resti Fitri, Afifah Ismu Fronica, Imelda Gilang Amanda Hafizah Fadhilah Hafizh Alza Afra Helsa Rahmatika Herisanti, Dini Indiastri P Monica Indria Palupi Irma Leilani Eka Putri Iyan Robiansyah Iyan Robiansyah Jalilah Azizah Jumatul Hafsah Kardiman, Reki Khairani, Fidia Aura Khairani, Salsabila Putri Linda Advinda Marizky Siregar, Yoana Marten, Threo Wanda Moralita Chatri Muhammad Farikh Muharani, Silvia Mukhlis Mukhlis Mutia Andini, Tri Nabilah, Rezi Nadira Nafisa Arini Nella Fauziah Novita, Yeni Nur Aqsha Nurfadillatun Nisa Wijaya Nurul Hasanah NURUL HIDAYAH Oliv Nurul Kanaya Nurul Kanaya Pinta, Sari Rahma Pradila, Andini Novalia Pramila, Cindy Pratama, Chelsylia Dara Pratama, Sandi Fransisco Pryatna, Muhamad Zacky Putri, Aulia Devani Putri, Irma Leilani Putri, Isna Aryunita Putri Putri, Santi Diana Putrizalda, Hafizhah Rafila, Zacki Rahmad Wanizal Pastha Rahmawati, Atika Ayu Rahmi Holinesti Rezeki Rival Alridho Rezi Nabilah Ria Fernanda, Irma Rijal Satria Rijal Satria Rini Wulandari Rinti Mutiara Sari Riza Umami Roza, Sri Yenica Ruri Fitriyani Ruri Fitriyani S. Syamsurizal safitri, fira Salsabil, Velina Salsabilla, Vishtari Sari Rahma Pinta Sari, Rinti Mutiara Satria, Rijal Sefina, Nadia Selaras, Ganda Hijrah Silvy Annisa Sisca Alicia Farma Suryani, Elisa Vauzia Vauzia Vauzia, Vauzia Vauzia, Vauzia Velina Salsabil Violita Violita Violita Violita Violita Violita Violita Yulita, Nelfi Yuni Ahda Yuni Ahda Zolkapli Eshak Zultsatunni’mah Zultsatunni’mah Zulyusri Zulyusri Zulyusri Zulyusri Zulyusri, Zulyusri Zulzusri, Zulzusri