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All Journal JURNAL KIMIA SAINS DAN APLIKASI Bioscience AL KAUNIYAH Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Martabe : Jurnal Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN TAMBUSAI Pelita Eksakta Jurnal Biogenerasi Journal of halal product and research (JHPR) 3BIO: Journal of Biological Science, Technology and Management Jurnal Jeumpa Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Promotor: Jurnal Mahasiswa Kesehatan Masyarakat Tropical Genetics Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi
Achyar, Afifatul
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang, Padang Indonesia
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Hydroponic Green Vegetable Cultivation Assistance in Support of SD Negeri 12 Padang Panjang’s Program Towards Achieving Adiwiyata School Violita, Violita; Holinesti, Rahmi; Sukma, Dina; Mukhlis, Mukhlis; Fronica, Imelda; Putri, Dwi Hilda; Achyar, Afifatul; Handayani, Dezi; Chatri, Moralita; Advinda, Linda; Farma, Siska Alicia; Nabilah, Rezi
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/291

Abstract

Abstract – SD Negeri 12 Padang Panjang is one of the schools that has made efforts to implement the Adiwiyata program. In 2024, the school represented the city of Padang Panjang in the Adiwiyata competition at the West Sumatra provincial level; however, it did not achieve optimal results. For the 2025 Adiwiyata program, SD Negeri 12 has set a target to become the provincial representative of West Sumatra. Based on the evaluation results from 2024, it was found that the assessment indicator for Participatory-Based Environmental Activities needs improvement. One activity that can support this effort is hydroponic cultivation. A hydroponic cultivation training was conducted by 26 fifth-grade students. The training included both theoretical material and hands-on practice in cultivating green vegetables using a hydroponic system. The training was guided by two experts who are competent in the field. This training successfully increased students' knowledge of hydroponic vegetable cultivation. Moreover, over 90% of the participants demonstrated teamwork and developed a greater awareness of environmental care. This outcome is a significant contribution to supporting SD Negeri 12 Padang Panjang Barat’s efforts toward achieving Adiwiyata School status.
Empowering Tourism Awareness Groups (Pokdarwis) through Biodiversity Education and Photography Training to Promote Conservation-Based Ecotourism in Singgalang Tandikek Nature Park Atifah, Yusni; Putri, Dwi Hilda; Achyar, Afifatul; Chatri, Moralita; Violita, Violita; Pratama, Sandi Fransisco; Vauzia, Vauzia; Putri, Irma Leilani; Putri, Aulia Devani
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/298

Abstract

At the present time, pressures on local ecosystems put many endemic species at risk of extinction if conservation measures are not promptly implemented. This community service program aimed to empower tourism awareness groups (Pokdarwis) in Singgalang Tandikek Nature Park through biodiversity education and photography training to support conservation-based ecotourism. The program targeted local guides, providing knowledge on endemic and protected species, particularly the Sumatran striped rabbit (Nesolagus netscheri), combined with practical photography skills using smartphones and ethical documentation practices. Activities included biodiversity lectures, hands-on photography, species identification, and social media promotion of local biodiversity. Evaluation using pre-test and post-tests showed an increase in participants’ photography knowledge (mean score 7.57 to 8.71), indicating improved awareness and skills in documenting biodiversity. The program successfully enhanced local capacity to promote sustainable ecotourism while fostering conservation ethics.
Analisis Filogenetik Bakteri Escherichia coli Berdasarkan DNA Genomik Gen BlaTEM Dari Data Sekuens NCBI: Pendekatan Bioinformatika terhadap Variasi Gen BlaTEM pada Escherichia coli Tahun 2016–2025 Palupi, Indria; Achyar, Afifatul
Tropical Genetics Vol. 5 No. 2 (2025): Genetics
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v5i2.102

Abstract

Resistensi antibiotik yang disebabkan oleh bakteri penghasil β-laktamase, seperti Escherichia coli, menjadi permasalahan global yang serius. Gen blaTEM merupakan salah satu gen utama yang berkontribusi terhadap resistensi tersebut. Penelitian ini bertujuan untuk menganalisis hubungan filogenetik antar isolat Escherichia coli berdasarkan sekuens DNA gen blaTEM yang diambil dari database NCBI dalam rentang waktu 2016 hingga 2025. Data sekuens diselaraskan menggunakan metode ClustalW dalam perangkat lunak MEGA X, kemudian dianalisis menggunakan metode Neighbor-Joining dan 1000 bootstrap. Hasil analisis menunjukkan pembentukan beberapa klade utama dengan tingkat kedekatan genetik yang bervariasi. Kelompok isolat tahun 2021–2024 memiliki kedekatan genetik tinggi, sedangkan isolat tahun 2020 dan 2025 menunjukkan divergensi yang signifikan. Temuan ini mengindikasikan adanya variasi evolusioner dalam gen blaTEM yang penting untuk dipantau sebagai bagian dari strategi pengendalian resistensi antibiotik.
The Effect of miR-10b Mimic Transfection on Invasion and Proliferation Gene Expression as the Direct Target of the miR-10b Pathway in T47D Breast Cancer Cell Lines Arisanty, Dessy; Eshak, Zolkapli; Wahyuni, Fatma Sri; Achyar, Afifatul; Khairani, Salsabila Putri
Jurnal Kimia Sains dan Aplikasi Vol 29, No 1 (2026): Volume 29 Issue 1 Year 2026
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.29.1.58-63

Abstract

Breast cancer remains one of the leading causes of death in women worldwide, with high incidence and mortality rates. The abnormal expression of the small non-coding RNA, microRNA-10b (miR-10b), has been associated with breast cancer progression. miR-10b is involved in various biological processes, including proliferation, metastasis, and drug resistance. However, the underlying mechanism by which miR-10b contributes to breast cancer progression remains unclear. This study aimed to investigate the effect of miR-10b on the expression of HOXD10 and PI3K genes, as well as its impact on cell viability and proliferation in breast cancer cells. In this study, T47D breast cancer cells were transfected with mimic miR-10b using the FANA-oligo transfection method at concentrations of 1, 2.5, and 5 µM. Cell viability was assessed using the MTT assay, while HOXD10 and PI3K gene expression levels were measured using RT-qPCR and ELISA. Data were analysed using paired t-tests with SPSS, and results were considered statistically significant at p < 0.05. The results showed that mimic miR-10b induced a dose-dependent increase in cell proliferation. Notably, only HOXD10 gene expression was significantly affected by the transfection, while PI3K gene expression remained unchanged. Cells transfected with 5 µM miR-10b mimic exhibited the highest proliferation rate compared to non-transfected controls (p < 0.05). In conclusion, miR-10b promotes breast cancer cell proliferation by downregulating HOXD10 expression, independent of PI3K, suggesting it is a potential target for further investigation.
Co-Authors Afionita, Santi Ahmad Hambali, Ahmad Ahmad Wibisana, Ahmad Alvenaya Hindayageni Ananda Putri, Ananda Annisa Irna Putri Annisa Khaira Annisa Khaira Annisa, Silvy Aprilia, Amanda Ara, Farrah Azzahra Ardi Ardi Atifah, Yusni Aura Zahra Nafisah Azizah, Jalilah Bintang Fadhil Ramadhan Cici Mustika cynthia perdana putri Dara Suci Amini, Dara Suci Des M Dessy Arisanty Dezi Handayani Dezi Handayani Dina Sukma Dwi Hilda Putri Dwi Hilda Putri Dwi Hilda Putri Edwin Edwin Elsa Badriyya Elsa Yuniarti Elviana, Alifah Hazelia Fadhila Humaira Fardilla, Midratul Fatiha, Fathma Dwi Fatma Sri Wahyuni Fevria, Resti Fitri, Afifah Ismu Fronica, Imelda Gilang Amanda Hafizah Fadhilah Hafizh Alza Afra Helsa Rahmatika Herisanti, Dini Irma Leilani Eka Putri Iyan Robiansyah Iyan Robiansyah Jalilah Azizah Jumatul Hafsah Kardiman, Reki Khairani, Fidia Aura Khairani, Salsabila Putri Linda Advinda Marten, Threo Wanda Monica , Indiastri P Moralita Chatri Muhammad Farikh Muharani, Silvia Mukhlis Mukhlis Mutia Andini, Tri Nabilah, Rezi Nadira Nafisa Arini Nella Fauziah Novita, Yeni Nur Aqsha Nurfadillatun Nisa Wijaya Nurul Hasanah NURUL HIDAYAH Oliv Nurul Kanaya Nurul Kanaya Palupi, Indria Pinta, Sari Rahma Pradila, Andini Novalia Pramila, Cindy Pratama, Chelsylia Dara Pratama, Sandi Fransisco Pryatna, Muhamad Zacky Putri, Aulia Devani Putri, Irma Leilani Putri, Isna Aryunita Putri Putri, Santi Diana Putrizalda, Hafizhah Rahmad Wanizal Pastha Rahmawati, Atika Ayu Rahmi Holinesti Rezeki Rival Alridho Rezi Nabilah Ria Fernanda, Irma Rijal Satria Rijal Satria Rini Wulandari Rinti Mutiara Sari Riza Umami Roza, Sri Yenica Ruri Fitriyani Ruri Fitriyani S. Syamsurizal safitri, fira Salsabil, Velina Salsabilla, Vishtari Sari Rahma Pinta Sari, Rinti Mutiara Satria, Rijal Sefina, Nadia Selaras, Ganda Hijrah Sisca Alicia Farma Suryani, Elisa Vauzia Vauzia Vauzia, Vauzia Velina Salsabil Violita Violita Violita Violita Violita Violita Violita Yulita, Nelfi Yuni Ahda Yuni Ahda Zolkapli Eshak Zultsatunni’mah Zultsatunni’mah Zulyusri Zulyusri Zulyusri Zulyusri Zulyusri, Zulyusri Zulzusri, Zulzusri