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<![CDATA[DESAIN PRIMER GEN EDNRB EKSON 4 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG DISEASE ]]>
<![CDATA[Hidayah, Nurul]]>;
<![CDATA[Ahda, Yuni]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Atifah, Yusni]]>
<![CDATA[ Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues) ]]>
Publisher : <![CDATA[Universitas Cokroaminoto Palopo ]]>
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DOI: 10.30605/biogenerasi.v10i2.5954
<![CDATA[EDNRB gene is a gene located on chromosome 13q22, plays a role in the signaling pathway during the development of the ENS intestinal nerves. Mutations in the EDNRB gene in humans are associated with Hirschsprung Disease (HD). Mutations in the EDNRB gene are the second most common cause after the RET gene in cases of HD with a percentage of 5–10%. To detect mutations, PCR products are needed. Primers are one of the basic components of PCR, while optimization of annealing temperature and optimization of primer concentration are factors in the success of primer attachment to the target gene. This study aims to design EDNRB exon 4 gene primers and determine the optimum annealing temperature and primer concentration to amplify the EDNRB gene. Primer design with Geneious Prime, and good primer criteria are analyzed in silico. Optimization of annealing temperature and primer concentration is carried out with gradient PCR and variations in primer concentration. The results of this study obtained the best primers, namely the forward primer 5'- CAGTAAGTGTGGCCTGAAAG-3' and the reverse primer 5'-GTGGAACCGAAGTGACTAGA-3', which amplified the EDNRB gene exon 4. The optimum PCR conditions for amplification of exon 4 of the EDNRB gene were at an annealing temperature of 57.2 ℃ and at a primer concentration of 0.5 μM.]]>
<![CDATA[Optimization of Authentication Methods for Processed Chicken Meat Products Based on ND5 Gene qPCR ]]>
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Safitri, Fira]]>;
<![CDATA[Atifah, Yusni]]>;
<![CDATA[Farma, Siska Alicia]]>;
<![CDATA[Putri, Dwi Hilda]]>;
<![CDATA[Ahda, Yuni]]>
<![CDATA[ Halal Research Vol 5 No 2 (2025): July ]]>
Publisher : <![CDATA[Halal Center ITS ]]>
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DOI: 10.12962/j22759970.v5i2.1823
<![CDATA[Consumers have the right to correct information about the products they consume. One example of a food product that is vulnerable to counterfeiting is chicken nuggets. To overcome this problem, qPCR can be an effective solution. Previous research has designed primers targeting the chicken ND5 gene. However, the PCR conditions for this primer pair have never been optimized. This research aims to optimize qPCR conditions and carry out chicken nugget authentication trials. Genomic DNA was isolated from five weight variations of chicken meat samples as a qPCR standard curve and three chicken nugget samples. Optimizing the annealing temperature with gradient PCR gave the best results at a temperature of 57.6OC. The results of the authentication test showed that the chicken meat content in TD nuggets was 54.7%, C nuggets 43.8%, and D nuggets 37.3%. This value is still in accordance with the Indonesian National Standard (SNI).]]>
<![CDATA[Optimisation of 25-OH D Elisa Kit Usage Using EDTA, Heparin, and Gel Blood Tubes ]]>
<![CDATA[Sefina, Nadia]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Wahyuni, Ira]]>
<![CDATA[ PROMOTOR Vol. 8 No. 5 (2025): OKTOBER ]]>
Publisher : <![CDATA[Universitas Ibn Khaldun Bogor ]]>
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DOI: 10.32832/pro.v8i5.1388
<![CDATA[The use of anticoagulants in blood collection tubes is also an important factor in the measurement of vitamin D and its metabolites. Vitamin D levels in circulating blood can be measured by measuring 25-hydroxyvitamin D levels in blood. This study aims to compare the effectiveness of using EDTA, heparin, and gel blood tubes in maintaining the stability of 25-OH D in blood samples. The type of research used is Analytical Descriptive research. The samples used in this study used venous blood which was determined using the Simple Random Sampling technique. Samples were taken from 5 semester S1 internship students totalling three people to take blood. Where the blood was taken as much as 3 cc and divided into several purple, green and yellow vacuntainer tubes. From the data obtained, it shows that the use of vacutainer tubes in blood storage to obtain serum for the most optimum vitamin D level test is in a tube with a green lid containing lithium heparin anticoagulant. Where the results obtained on lithium heparin anticoagulant are in the range of >60 ng/mL. As strong evidence of no potential adverse effects at high (optimal) levels. Tubes with purple or lavender coloured covers containing EDTA had results <20 ng/mL indicating that vitamin D deficiency may occur due to lack of vitamin D nutrition. On the other hand, the tube with yellow coloured lid containing anticoagulant gel showed >100 ng/mL indicating vitamin D excess.]]>
<![CDATA[Dextran Producing Bacteria: A Literatur Review ]]>
<![CDATA[Salsabilla, Vishtari]]>;
<![CDATA[Putri, Dwi Hilda]]>;
<![CDATA[Advinda, Linda]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Wibisana, Ahmad]]>
<![CDATA[ Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret ]]>
Publisher : <![CDATA[Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia ]]>
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DOI: 10.29303/jbt.v25i1.8370
<![CDATA[This study aims to analyze dextran-producing bacteria, especially from the general Leuconostoc, Weissella, and Lactobacillus, which produce the enzyme dextransucrase for dextran synthesis. This research uses the Systematic Literature Review (SLR) method based on the PRISMA protocol, with data sources from various trusted scientific databases for the 2020–2024 period. The results of the study show that variations in dextran structure, such as molecular weight and degree of branching, are highly dependent on the bacterial strain and fermentation conditions. Optimization of production parameters, including pH, temperature and substrate concentration, can increase dextran production efficiency The conclusions of this study emphasize the need for a multidisciplinary approach to optimize the potential of dextran in industrial and biotechnology applications. Further research is recommended to explore new strains, optimize production processes, and diversify dextran applications.]]>
<![CDATA[Jenis-Jenis Capung (Odonata) Pada Beberapa Tipe Habitat di Korong Asam Pulau, Kabupaten Padang Pariaman, Sumatera Barat ]]>
<![CDATA[Aprilia, Amanda]]>;
<![CDATA[Pratama, Sandi Fransisco]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Satria, Rijal]]>
<![CDATA[ Jurnal Pendidikan Tambusai Vol. 9 No. 1 (2025) ]]>
Publisher : <![CDATA[LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia ]]>
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DOI: 10.31004/jptam.v9i1.26460
<![CDATA[Capung (Odonata) merupakan serangga yang berperan penting sebagai bioindikator kualitas lingkungan. Penelitian ini bertujuan untuk mengidentifikasi jenis capung pada beberapa tipe habitat, yaitu sungai, sawah, hutan, dan lapangan berumput di Korong Asam Pulau, Kabupaten Padang Pariaman, Sumatera Barat. Pengambilan data dilakukan dengan menggunakan metode Visual Encounter Survey (VES) pada plot berukuran 20m x 20m. Hasil penelitian menunjukkan ditemukannya 14 jenis, 11 genera, 6 famili, dan 79 individu. Subordo Anisoptera ditemukan pada semua tipe habitat dan Subordo Zygoptera hanya ditemukan pada habitat hutan dan sungai saja. Kehadiran capung pada suatu tipe habitat dipengaruhi oleh karakteristik dari masing-masing habitat. Penelitian ini juga mengkonfirmasi bahwa spesies subordo Zygoptera lebih sensitif terhadap perubahan lingkungan dibandingkan Anisoptera. Hal ini menunjukkan bahwa capung dapat digunakan sebagai bioindikator kualitas lingkungan dan tingkat gangguan ekosistem akibat aktivitas manusia.]]>
<![CDATA[Analisis pohon filogenetik gen matK Ageratum conyzoides di Beberapa Negara ]]>
<![CDATA[Ria Fernanda, Irma]]>;
<![CDATA[Umami, Riza]]>;
<![CDATA[Achyar, Afifatul]]>
<![CDATA[ Jurnal Pendidikan Tambusai Vol. 9 No. 2 (2025): Agustus ]]>
Publisher : <![CDATA[LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia ]]>
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DOI: 10.31004/jptam.v9i2.31136
<![CDATA[Ageratum conyzoides L. (Asteraceae) adalah herba aromatik yang tersebar di daerah tropis dan subtropis. A. conyzoidestelah memberikan banyak kegunaan etnomedisinal karena telah digunakan untuk menyembuhkan berbagai penyakit yang meliputi kusta, gangguan kulit, penyakit tidur, rematik, sakit kepala, dispnea, sakit gigi, pneumonia dan banyak lagi. Penelitian ini bertujuan untuk mengetahui hubungan kekeraatan spesie Ageratum conyzoides dengan analisis filogenetik berdasarkan sekuen gen matK di berbagai negara. Pengambilan data penelitian menggunakan data sekunder sekuen Ageratum conyzoides dari database GenBank di National Center for Biology Information (NCBI) berupa sekuens nukleotida berupa gene MatK yang diunduh dalam format FASTA. Analisis filogenetik dengan metode Maximum Likelihood. Konstruksi dilakukan dengan perangkat lunak MEGA 11. Hasil analisis filogenetik ditemukan bahwa spesies A. Conyzoides yang berada di Kenya berbeda dengan A. Conyzoides di wilayah Asia. Hal ini dapat disebabkan oleh letak geografis yang luas serta adanya batas wilayah yang menyebabkan spesies tersebut identik dengan daerah masing-masing.]]>
<![CDATA[Primer Design and Optimization of Annealing Temperature for Gene Amplification GSTL2 on Rice ]]>
<![CDATA[Violita, Violita]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Zulyusri, Zulyusri]]>;
<![CDATA[Atifah, Yusni]]>;
<![CDATA[Putri, Dwi Hilda]]>;
<![CDATA[Nabilah, Rezi]]>
<![CDATA[ Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami ]]>
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DOI: 10.15408/kauniyah.v17i2.32859
<![CDATA[AbstractGlutathione S-Transferase is a superfamily enzyme that has many roles for living things, especially in the detoxification of Reactive Oxygen Species (ROS), one of which is rice plants. One gene of this family that has a role for plants is GSTL2 This gene is known to have a contribution to Arabidopsis and Oryza sativa L. against abiotic stress. To find out how this gene expression is requires a primer to specifically amplify this gene, and an optimal annealing temperature to support the success of the primer. This study aims to design primers and determine the optimal annealing temperature for gene amplification GSTL2. Primers were designed using the Primer3, which were then analyzed with Genious Prime based on good primer criteria and optimizing the annealing temperature which was carried out using gradient PCR. The results of this study obtained a primer pair is forward 5’-TTCGAAGGGCCAGCATTACT-3’ primer reverse 5’-CAATGTCCACCAAGCTGAA-3’. The primer pair has a length of 20 nt, with melting temperature (Tm) 59–59,4 °C, and GC content 50%. The primer forward there is a secondary structure in the form of a self-dimer with (Tm) 6.2 °C. The primer pair can amplify gene sequences GSTL2 by producing a PCR product 224 bp. The annealing temperature of 60 °C resulting in a single thick, bright DNA strand.AbstrakGlutathione S-Transferase merupakan enzim superfamily yang memiliki banyak peranan bagi makhluk hidup terutama dalam detoksifikasi Reactive Oxygen Species (ROS), salah satunya tumbuhan. Salah satu gen dari famili ini yang memiliki peranan bagi tanaman adalah GSTL2. Gen ini diketahui memiliki kontribusi bagi tanaman Arabidopsis dan Oryza sativa L. terhadap stres abiotik. Untuk mengetahui bagaimana ekspresi gen GSTL2 ini dibutuhkan primer untuk mengamplifikasi gen ini secara spesifik, di samping itu suhu annealing yang optimal untuk menunjang keberhasilan primer. Penelitian ini bertujuan untuk mendesain primer dan menentukan suhu annealing yang optimal untuk mengamplifikasi gen GSTL2. Primer didesain menggunakan tools Pick Primer dan Geneious Prime, yang kemudian dianalisis secara in silico berdasarkan kriteria primer yang baik. Serta optimasi suhu annealing yang dilakukan menggunakan gradient PCR. Hasil penelitian ini diperoleh sepasang primer dengan panjang masing-masing primer 20 nt, primer forward 5’-TTCGAAGGGCCAGCATTACT-3’ primer reverse 5’-CAATGTCCACCAAGCTGAA-3’. Pasangan primer dapat mengamplifikasi sekuen gen GSTL2 dengan menghasilkan produk PCR sebesar 224 bp pada suhu annealing 60 °C.]]>
<![CDATA[EDUKASI JAJANAN SEHAT KEPADA SISWA SD DALAM MENGHADAPI ERA NEW NORMAL ]]>
<![CDATA[Atifah, Yusni]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Satria, Rijal]]>;
<![CDATA[Violita, Violita]]>;
<![CDATA[Rahmatika, Helsa]]>;
<![CDATA[Azizah, Jalilah]]>
<![CDATA[ Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 6, No 1 (2023): Martabe : Jurnal Pengabdian Kepada Masyarakat ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Tapanuli Selatan ]]>
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DOI: 10.31604/jpm.v6i1.213-217
<![CDATA[Anak usia sekolah memerlukan asupan energi dan zat gizi untuk mendukung pertumbuhan dan juga prestasi belajar anak di sekolah. Salah satu sumber asupan energi dan zat gizi bagi anak sekolah bersumber dari pangan jajanan. Anak usia sekolah sebagai generasi penerus bangsa merupakan investasi bagi suatu bangsa. Kualitas anak-anak pada masa sekarang akan menentukan kualitas suatu negara di masa depan. Edukasi keamanan pangan jajanan perlu dilakukan sejak dini secara sistematis dan juga berkelanjutan sebagai salah satu upaya untuk peningkatan kualitas sumber daya manusia. Pemberian nutrisi yang berkualitas, sehat dan sesuai dengan kuantitas yang seharusnya penting untuk menunjang pertumbuhan perkembangan anak usia sekolah agar optimal. Oleh karena itu, perlu adanya edukasi kepada siswa SD untuk meningkatkan pengetahuan terkait keamanan pangan jajanan dan mengetahui arti simbol-simbol pada bungkus jajanan. Kegiatan ini dilaksanakan pada bulan Juli 2022, dan diikuti oleh 206 peserta dari kelas 3 dan 6 SDN 02 Payakumbuh. Hasil evaluasi kegiatan menunjukkan adanya peningkatan pengetahuan siswa terhadap keamananan jajanan dan memiliki keterampilan dalam mengidentifikasi simbol-simbol pada bungkus jajanan. Kesimpulan untuk kegiatan ini sudah efektif dilaksanakan dan dapat mengedukasi siswa SD dalam mengetahui keamanan pangan jajanan.]]>
<![CDATA[Pengaruh Penambahan Cabai ( Capsicum annum ) Dan Gula Terhadap Total Bakteri Asam Laktat (BAL) Dari Sauerkraut ]]>
<![CDATA[Vauzia, Vauzia]]>;
<![CDATA[Fevria, Resti]]>;
<![CDATA[Monica , Indiastri P]]>;
<![CDATA[Chatri, Moralita]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Edwin, Edwin]]>
<![CDATA[ Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic ]]>
Publisher : <![CDATA[Postgraduate, University of Mataram ]]>
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DOI: 10.29303/jppipa.v9iSpecialIssue.4811
<![CDATA[Sauerkraut is a typical German food made from thinly sliced cabbage. Sauerkraut is a fermeted product that can give a distinctive taste. Sugar is an ingredient that can be added in, that sugar can trigger the growth of lactic acid bacteria. Indonesian people like food with a spicy sensation from chilies, chillies here contain vitamin C which functions as an antioxidant that is good for the body and is able to increase endurance. This study aims to calculate the total lactic acid bacteria contained in sauerkraut with the addition of chilies and sugar. Dilution of 1 ml sample into 9 ml physiological NaCl solution from 10 -1 to 10 -8 dilutions was carried out, then inoculated into MRS Agar using the pour plate method. Incubated in room temperature for 48 hours. The growing colonies of lactic acid bacteria were then observed based on colony morphology and counting the total growing lactic acid bacteria. The results of the research can be concluded that there was an increase in the number of lactic acid bacteria in sauerkraut with the addition of chili and 10% granulated sugar with a bacterial density average is 88.667 x 108 CFU/mL.]]>
<![CDATA[Strengthening the Usaha Tani group Nagari Sungai Abang as organic center in Subdistrict of Lubuk Alung Padang Pariaman ]]>
<![CDATA[Kardiman, Reki]]>;
<![CDATA[Achyar, Afifatul]]>;
<![CDATA[Selaras, Ganda Hijrah]]>;
<![CDATA[Novita, Yeni]]>;
<![CDATA[Fardilla, Midratul]]>;
<![CDATA[Roza, Sri Yenica]]>
<![CDATA[ Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2 ]]>
Publisher : <![CDATA[Fakultas MIPA Universitas Negeri Padang ]]>
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DOI: 10.24036/pelitaeksakta/vol8-iss2/277
<![CDATA[Back to organic farming is necessary but applying this should begin with production of the organic products in a special place called organic center, where the all possible organic products produced from organic waste in a place. This approach has started by the Usaha Tani group last year but failed in fulfilling the goals. This program was proposed to strengthening the organic center with some simple organic projects such as liquid organic fertilizer (LOF), eco-enzyme and maggot. The activities were conducted through establishing the facilities and enhancing the skills. Three of 100 kg buckets were established and were produced about 60 liter LOF, and eco-enzyme is now producing about 1680 liter within 15 of 25 kg buckets, while 450 maggots were ready to be released for making hundred thousand eggs. The farmer group is very antusias with the process and the promissing yields, and be ready for the next steps.]]>
