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All Journal JURNAL KIMIA SAINS DAN APLIKASI Bioscience AL KAUNIYAH Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Indonesian Food Science and TechnologyJournal Martabe : Jurnal Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN TAMBUSAI Pelita Eksakta Jurnal Biogenerasi Journal of halal product and research (JHPR) 3BIO: Journal of Biological Science, Technology and Management Jurnal Jeumpa Halal Research Journal TROPICAL GENETICS MASALIQ: Jurnal Pendidikan dan Sains Promotor: Jurnal Mahasiswa Kesehatan Masyarakat Tropical Genetics Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi
Achyar, Afifatul
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang, Padang Indonesia
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DESAIN PRIMER GEN EDNRB EKSON 4 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG DISEASE Hidayah, Nurul; Ahda, Yuni; Achyar, Afifatul; Atifah, Yusni
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5954

Abstract

EDNRB gene is a gene located on chromosome 13q22, plays a role in the signaling pathway during the development of the ENS intestinal nerves. Mutations in the EDNRB gene in humans are associated with Hirschsprung Disease (HD). Mutations in the EDNRB gene are the second most common cause after the RET gene in cases of HD with a percentage of 5–10%. To detect mutations, PCR products are needed. Primers are one of the basic components of PCR, while optimization of annealing temperature and optimization of primer concentration are factors in the success of primer attachment to the target gene. This study aims to design EDNRB exon 4 gene primers and determine the optimum annealing temperature and primer concentration to amplify the EDNRB gene. Primer design with Geneious Prime, and good primer criteria are analyzed in silico. Optimization of annealing temperature and primer concentration is carried out with gradient PCR and variations in primer concentration. The results of this study obtained the best primers, namely the forward primer 5'- CAGTAAGTGTGGCCTGAAAG-3' and the reverse primer 5'-GTGGAACCGAAGTGACTAGA-3', which amplified the EDNRB gene exon 4. The optimum PCR conditions for amplification of exon 4 of the EDNRB gene were at an annealing temperature of 57.2 ℃ and at a primer concentration of 0.5 μM.
Optimization of Authentication Methods for Processed Chicken Meat Products Based on ND5 Gene qPCR Achyar, Afifatul; Safitri, Fira; Atifah, Yusni; Farma, Siska Alicia; Putri, Dwi Hilda; Ahda, Yuni
Halal Research Vol 5 No 2 (2025): July
Publisher : Halal Center ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j22759970.v5i2.1823

Abstract

Consumers have the right to correct information about the products they consume. One example of a food product that is vulnerable to counterfeiting is chicken nuggets. To overcome this problem, qPCR can be an effective solution. Previous research has designed primers targeting the chicken ND5 gene. However, the PCR conditions for this primer pair have never been optimized. This research aims to optimize qPCR conditions and carry out chicken nugget authentication trials. Genomic DNA was isolated from five weight variations of chicken meat samples as a qPCR standard curve and three chicken nugget samples. Optimizing the annealing temperature with gradient PCR gave the best results at a temperature of 57.6OC. The results of the authentication test showed that the chicken meat content in TD nuggets was 54.7%, C nuggets 43.8%, and D nuggets 37.3%. This value is still in accordance with the Indonesian National Standard (SNI).
Optimisation of 25-OH D Elisa Kit Usage Using EDTA, Heparin, and Gel Blood Tubes Sefina, Nadia; Achyar, Afifatul; Wahyuni, Ira
PROMOTOR Vol. 8 No. 5 (2025): OKTOBER
Publisher : Universitas Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/pro.v8i5.1388

Abstract

The use of anticoagulants in blood collection tubes is also an important factor in the measurement of vitamin D and its metabolites. Vitamin D levels in circulating blood can be measured by measuring 25-hydroxyvitamin D levels in blood. This study aims to compare the effectiveness of using EDTA, heparin, and gel blood tubes in maintaining the stability of 25-OH D in blood samples. The type of research used is Analytical Descriptive research. The samples used in this study used venous blood which was determined using the Simple Random Sampling technique. Samples were taken from 5 semester S1 internship students totalling three people to take blood. Where the blood was taken as much as 3 cc and divided into several purple, green and yellow vacuntainer tubes. From the data obtained, it shows that the use of vacutainer tubes in blood storage to obtain serum for the most optimum vitamin D level test is in a tube with a green lid containing lithium heparin anticoagulant. Where the results obtained on lithium heparin anticoagulant are in the range of >60 ng/mL. As strong evidence of no potential adverse effects at high (optimal) levels. Tubes with purple or lavender coloured covers containing EDTA had results <20 ng/mL indicating that vitamin D deficiency may occur due to lack of vitamin D nutrition. On the other hand, the tube with yellow coloured lid containing anticoagulant gel showed >100 ng/mL indicating vitamin D excess.
Dextran Producing Bacteria: A Literatur Review Salsabilla, Vishtari; Putri, Dwi Hilda; Advinda, Linda; Achyar, Afifatul; Wibisana, Ahmad
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8370

Abstract

This study aims to analyze dextran-producing bacteria, especially from the general Leuconostoc, Weissella, and Lactobacillus, which produce the enzyme dextransucrase for dextran synthesis. This research uses the Systematic Literature Review (SLR) method based on the PRISMA protocol, with data sources from various trusted scientific databases for the 2020–2024 period. The results of the study show that variations in dextran structure, such as molecular weight and degree of branching, are highly dependent on the bacterial strain and fermentation conditions. Optimization of production parameters, including pH, temperature and substrate concentration, can increase dextran production efficiency The conclusions of this study emphasize the need for a multidisciplinary approach to optimize the potential of dextran in industrial and biotechnology applications. Further research is recommended to explore new strains, optimize production processes, and diversify dextran applications.
Jenis-Jenis Capung (Odonata) Pada Beberapa Tipe Habitat di Korong Asam Pulau, Kabupaten Padang Pariaman, Sumatera Barat Aprilia, Amanda; Pratama, Sandi Fransisco; Achyar, Afifatul; Satria, Rijal
Jurnal Pendidikan Tambusai Vol. 9 No. 1 (2025)
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v9i1.26460

Abstract

Capung (Odonata) merupakan serangga yang berperan penting sebagai bioindikator kualitas lingkungan. Penelitian ini bertujuan untuk mengidentifikasi jenis capung pada beberapa tipe habitat, yaitu sungai, sawah, hutan, dan lapangan berumput di Korong Asam Pulau, Kabupaten Padang Pariaman, Sumatera Barat. Pengambilan data dilakukan dengan menggunakan metode Visual Encounter Survey (VES) pada plot berukuran 20m x 20m. Hasil penelitian menunjukkan ditemukannya 14 jenis, 11 genera, 6 famili, dan 79 individu. Subordo Anisoptera ditemukan pada semua tipe habitat dan Subordo Zygoptera hanya ditemukan pada habitat hutan dan sungai saja. Kehadiran capung pada suatu tipe habitat dipengaruhi oleh karakteristik dari masing-masing habitat. Penelitian ini juga mengkonfirmasi bahwa spesies subordo Zygoptera lebih sensitif terhadap perubahan lingkungan dibandingkan Anisoptera. Hal ini menunjukkan bahwa capung dapat digunakan sebagai bioindikator kualitas lingkungan dan tingkat gangguan ekosistem akibat aktivitas manusia.
Analisis pohon filogenetik gen matK Ageratum conyzoides di Beberapa Negara Ria Fernanda, Irma; Umami, Riza; Achyar, Afifatul
Jurnal Pendidikan Tambusai Vol. 9 No. 2 (2025): Agustus
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v9i2.31136

Abstract

Ageratum conyzoides L. (Asteraceae) adalah herba aromatik yang tersebar di daerah tropis dan subtropis. A. conyzoidestelah memberikan banyak kegunaan etnomedisinal karena telah digunakan untuk menyembuhkan berbagai penyakit yang meliputi kusta, gangguan kulit, penyakit tidur, rematik, sakit kepala, dispnea, sakit gigi, pneumonia dan banyak lagi. Penelitian ini bertujuan untuk mengetahui hubungan kekeraatan spesie Ageratum conyzoides dengan analisis filogenetik berdasarkan sekuen gen matK di berbagai negara. Pengambilan data penelitian menggunakan data sekunder sekuen Ageratum conyzoides dari database GenBank di National Center for Biology Information (NCBI) berupa sekuens nukleotida berupa gene MatK yang diunduh dalam format FASTA. Analisis filogenetik dengan metode Maximum Likelihood. Konstruksi dilakukan dengan perangkat lunak MEGA 11. Hasil analisis filogenetik ditemukan bahwa spesies A. Conyzoides yang berada di Kenya berbeda dengan A. Conyzoides di wilayah Asia. Hal ini dapat disebabkan oleh letak geografis yang luas serta adanya batas wilayah yang menyebabkan spesies tersebut identik dengan daerah masing-masing.
Primer Design and Optimization of Annealing Temperature for Gene Amplification GSTL2 on Rice Violita, Violita; Achyar, Afifatul; Zulyusri, Zulyusri; Atifah, Yusni; Putri, Dwi Hilda; Nabilah, Rezi
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.32859

Abstract

AbstractGlutathione S-Transferase is a superfamily enzyme that has many roles for living things, especially in the detoxification of Reactive Oxygen Species (ROS), one of which is rice plants. One gene of this family that has a role for plants is GSTL2 This gene is known to have a contribution to Arabidopsis and Oryza sativa L. against abiotic stress. To find out how this gene expression is requires a primer to specifically amplify this gene, and an optimal annealing temperature to support the success of the primer. This study aims to design primers and determine the optimal annealing temperature for gene amplification GSTL2. Primers were designed using the Primer3, which were then analyzed with Genious Prime based on good primer criteria and optimizing the annealing temperature which was carried out using gradient PCR. The results of this study obtained a primer pair is forward 5’-TTCGAAGGGCCAGCATTACT-3’ primer reverse 5’-CAATGTCCACCAAGCTGAA-3’. The primer pair has a length of 20 nt, with melting temperature (Tm) 59–59,4 °C, and GC content 50%. The primer forward there is a secondary structure in the form of a self-dimer with (Tm) 6.2 °C. The primer pair can amplify gene sequences GSTL2 by producing a PCR product 224 bp. The annealing temperature of 60 °C resulting in a single thick, bright DNA strand.AbstrakGlutathione S-Transferase merupakan enzim superfamily yang memiliki banyak peranan bagi makhluk hidup terutama dalam detoksifikasi Reactive Oxygen Species (ROS), salah satunya tumbuhan. Salah satu gen dari famili ini yang memiliki peranan bagi tanaman adalah GSTL2. Gen ini diketahui memiliki kontribusi bagi tanaman Arabidopsis dan Oryza sativa L. terhadap stres abiotik. Untuk mengetahui bagaimana ekspresi gen GSTL2 ini dibutuhkan primer untuk mengamplifikasi gen ini secara spesifik, di samping itu suhu annealing yang optimal untuk menunjang keberhasilan primer. Penelitian ini bertujuan untuk mendesain primer dan menentukan suhu annealing yang optimal untuk mengamplifikasi gen GSTL2. Primer didesain menggunakan tools Pick Primer dan Geneious Prime, yang kemudian dianalisis secara in silico berdasarkan kriteria primer yang baik. Serta optimasi suhu annealing yang dilakukan menggunakan gradient PCR. Hasil penelitian ini diperoleh sepasang primer dengan panjang masing-masing primer 20 nt, primer forward 5’-TTCGAAGGGCCAGCATTACT-3’ primer reverse 5’-CAATGTCCACCAAGCTGAA-3’. Pasangan primer dapat mengamplifikasi sekuen gen GSTL2 dengan menghasilkan produk PCR sebesar 224 bp pada suhu annealing 60 °C.
Strengthening the Usaha Tani group Nagari Sungai Abang as organic center in Subdistrict of Lubuk Alung Padang Pariaman Kardiman, Reki; Achyar, Afifatul; Selaras, Ganda Hijrah; Novita, Yeni; Fardilla, Midratul; Roza, Sri Yenica
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/277

Abstract

Back to organic farming is necessary but applying this should begin with production of the organic products in a special place called organic center, where the all possible organic products produced from organic waste in a place. This approach has started by the Usaha Tani group last year but failed in fulfilling the goals. This program was proposed to strengthening the organic center with some simple organic projects such as liquid organic fertilizer (LOF), eco-enzyme and maggot. The activities were conducted through establishing the facilities and enhancing the skills. Three of 100 kg buckets were established and were produced about 60 liter LOF, and eco-enzyme is now producing about 1680 liter within 15 of 25 kg buckets, while 450 maggots were ready to be released for making hundred thousand eggs. The farmer group is very antusias with the process and the promissing yields, and be ready for the next steps.
Hydroponic Green Vegetable Cultivation Assistance in Support of SD Negeri 12 Padang Panjang’s Program Towards Achieving Adiwiyata School Violita, Violita; Holinesti, Rahmi; Sukma, Dina; Mukhlis, Mukhlis; Fronica, Imelda; Putri, Dwi Hilda; Achyar, Afifatul; Handayani, Dezi; Chatri, Moralita; Advinda, Linda; Farma, Siska Alicia; Nabilah, Rezi
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/291

Abstract

Abstract – SD Negeri 12 Padang Panjang is one of the schools that has made efforts to implement the Adiwiyata program. In 2024, the school represented the city of Padang Panjang in the Adiwiyata competition at the West Sumatra provincial level; however, it did not achieve optimal results. For the 2025 Adiwiyata program, SD Negeri 12 has set a target to become the provincial representative of West Sumatra. Based on the evaluation results from 2024, it was found that the assessment indicator for Participatory-Based Environmental Activities needs improvement. One activity that can support this effort is hydroponic cultivation. A hydroponic cultivation training was conducted by 26 fifth-grade students. The training included both theoretical material and hands-on practice in cultivating green vegetables using a hydroponic system. The training was guided by two experts who are competent in the field. This training successfully increased students' knowledge of hydroponic vegetable cultivation. Moreover, over 90% of the participants demonstrated teamwork and developed a greater awareness of environmental care. This outcome is a significant contribution to supporting SD Negeri 12 Padang Panjang Barat’s efforts toward achieving Adiwiyata School status.
Empowering Tourism Awareness Groups (Pokdarwis) through Biodiversity Education and Photography Training to Promote Conservation-Based Ecotourism in Singgalang Tandikek Nature Park Atifah, Yusni; Putri, Dwi Hilda; Achyar, Afifatul; Chatri, Moralita; Violita, Violita; Pratama, Sandi Fransisco; Vauzia, Vauzia; Putri, Irma Leilani; Putri, Aulia Devani
Pelita Eksakta Vol 8 No 2 (2025): Pelita Eksakta, Vol. 8, No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol8-iss2/298

Abstract

At the present time, pressures on local ecosystems put many endemic species at risk of extinction if conservation measures are not promptly implemented. This community service program aimed to empower tourism awareness groups (Pokdarwis) in Singgalang Tandikek Nature Park through biodiversity education and photography training to support conservation-based ecotourism. The program targeted local guides, providing knowledge on endemic and protected species, particularly the Sumatran striped rabbit (Nesolagus netscheri), combined with practical photography skills using smartphones and ethical documentation practices. Activities included biodiversity lectures, hands-on photography, species identification, and social media promotion of local biodiversity. Evaluation using pre-test and post-tests showed an increase in participants’ photography knowledge (mean score 7.57 to 8.71), indicating improved awareness and skills in documenting biodiversity. The program successfully enhanced local capacity to promote sustainable ecotourism while fostering conservation ethics.
Co-Authors Afionita, Santi Ahmad Hambali, Ahmad Ahmad Wibisana, Ahmad Alvenaya Hindayageni Ananda Putri, Ananda Annisa Irna Putri Annisa Khaira Annisa Khaira Aprilia, Amanda Ara, Farrah Azzahra Ardi Ardi Atifah, Yusni Aura Zahra Nafisah Bintang Fadhil Ramadhan Cici Mustika cynthia perdana putri Dara Suci Amini, Dara Suci Des M Dessy Arisanty Dezi Handayani Dezi Handayani Dina Sukma Dwi Hilda Putri Dwi Hilda Putri Dwi Hilda Putri Edwin Edwin Edwin Edwin Elsa Badriyya Elsa Yuniarti Elviana, Alifah Hazelia Fadhila Humaira Fardilla, Midratul Fatiha, Fathma Dwi Fatma Sri Wahyuni Fevria, Resti Fitri, Afifah Ismu Fronica, Imelda Gilang Amanda Hafizah Fadhilah Hafizh Alza Afra Helsa Rahmatika Herisanti, Dini Indiastri P Monica Indria Palupi Irma Leilani Eka Putri Iyan Robiansyah Iyan Robiansyah Jalilah Azizah Jumatul Hafsah Kardiman, Reki Khairani, Fidia Aura Khairani, Salsabila Putri Linda Advinda Marizky Siregar, Yoana Marten, Threo Wanda Moralita Chatri Muhammad Farikh Muharani, Silvia Mukhlis Mukhlis Mutia Andini, Tri Nabilah, Rezi Nadira Nafisa Arini Nella Fauziah Novita, Yeni Nur Aqsha Nurfadillatun Nisa Wijaya Nurul Hasanah NURUL HIDAYAH Oliv Nurul Kanaya Nurul Kanaya Pinta, Sari Rahma Pradila, Andini Novalia Pramila, Cindy Pratama, Chelsylia Dara Pratama, Sandi Fransisco Pryatna, Muhamad Zacky Putri, Aulia Devani Putri, Irma Leilani Putri, Isna Aryunita Putri Putri, Santi Diana Putrizalda, Hafizhah Rafila, Zacki Rahmad Wanizal Pastha Rahmawati, Atika Ayu Rahmi Holinesti Rezeki Rival Alridho Rezi Nabilah Ria Fernanda, Irma Rijal Satria Rijal Satria Rini Wulandari Rinti Mutiara Sari Riza Umami Roza, Sri Yenica Ruri Fitriyani Ruri Fitriyani S. Syamsurizal safitri, fira Salsabil, Velina Salsabilla, Vishtari Sari Rahma Pinta Sari, Rinti Mutiara Satria, Rijal Sefina, Nadia Selaras, Ganda Hijrah Silvy Annisa Sisca Alicia Farma Suryani, Elisa Vauzia Vauzia Vauzia, Vauzia Vauzia, Vauzia Velina Salsabil Violita Violita Violita Violita Violita Violita Violita Yulita, Nelfi Yuni Ahda Yuni Ahda Zolkapli Eshak Zultsatunni’mah Zultsatunni’mah Zulyusri Zulyusri Zulyusri Zulyusri Zulyusri, Zulyusri Zulzusri, Zulzusri