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37% Phosphoric Acid Induced Stronger Matrix Metalloproteinase-8 Expression of the Dental Pulp than 19% Ethylene Diamine Tetraacetic Acid Fatimatuzzahro, Nadie; Haniastuti, Tetiana; Handajani, Juni
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

37% Phosphoric Acid Induced Stronger Matrix Metalloproteinase-8 Expression of the Dental Pulp than 19% Ethylene Diamine Tetraacetic Acid Nadie Fatimatuzzahro; Tetiana Haniastuti; Juni Handajani
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Etching agents such as ethylene diamine tetraacetic acid (EDTA) and phosphoric acid which are widely used in adhesive restoration system aimed to increase for retention of restorative materials, may act a chemical irritant that induce inflammation of dental pulp. Inflammation is a body response against irritant and infectious agents. Matrix metalloproteinase-8, the major collagenolytic enzyme, degrades collagen type 1. This enzyme is expressed in low level in normal condition, however, the expression will increase during inflammation. The purpose of the present research was to study the effect of 19% EDTA and 37% phosphoric acid application as an etching agents on the MMP-8 expression of dental pulp. Forty-five male Sprague Dawley rats were divided into 3 groups. Cavity preparation was made on the occlusal surface of maxillary first molar using a round diamond bur. 19% EDTA, 37% phosphoric acid, and distilled water were applied on the surface of the cavity of the teeth in group I, II, and III subsequently. The cavity then filed by glass ionomer cements. The rats were sacrified at 1, 3, 5, 7, and 14 days after the treatment (n=3 for each day). The specimens were then processed histologically. Immunohistochemical (IHC) analysis was performed using rabbit anti rat MMP-8 polyclonal antibody to examine MMP-8 expression and HE (Hematoxylen Eosin) staining to observe the number of macrophages. The results showed 37% phos­phoric acid application induced stronger expression of MMP-8 and higher number of macrophages than 19% EDTA. The strongest expression of MMP-8 seems on 5 days after the treatment where the highest number of macrophages were also found.
Co-Authors Afifah Rufaida Alma Linggar Jonarta Annisa Rahmi Fauzia Antinah Latif, Antinah Anugerah Pekerti Astamurtiningrum Ardiny Andriani Aurita Siwi R Aurita Siwi R, Aurita Siwi Bambang Priyono Chynintia Dewi Aqmarina Dea Cahyani, Yessica Dhika Paramita Prameswari Dhika Paramita Prameswari, Dhika Paramita Dhinintya Hyta N Dhinintya Hyta N, Dhinintya Hyta Dhinintya Hyta Narissi Dyah Irnawati Ema Mulyawati Endah Kusumastuti Enggardipta, Raras Ajeng Evy Tri Utami Evy Tri Utami Evy Tri Utami Failasofia Failasofia Fery Setiawan Fery Setiawan Finsa Tisna Sari Finsa Tisna Sari, Finsa Tisna Fitri Setia Rahayu Haniastuti, Tetiana Hardono Jaya Lauson Heni Susilowati Heni Susilowati Heni Susilowati Icha Nofikasari Indah Fatikarini Islamy Rahma Hutami, Islamy Rahma Isti Endah Kurniwati Isti Endah Kurniwati, Isti Endah Juwita Raditya Ningsih Kristiyani Dwi M Kristiyani Dwi M, Kristiyani Dwi Lanagusti, Alfin Layung Sekar Prabarayi Lisdrianto Hanindriyo Lisdrianto Hanindriyo Lisna Kurnia Rezky Lukis, Prima Agusti Mahadna Aulia Rahmah Marsetyawan HNES Mochammad Imron Awalludin Moh. Khafid Mohammad Khafid Mufidana Azis Mufidana Azis, Mufidana Muhammad Nabil Pratama Nadia Rully Auliawati Nadie Fatimatuzzahro Norma Dias L Norma Dias L, Norma Dias Nuryono Nuryono Orienty, Fauzia Nilam Prakosa, Basma Rosandi Pramana Pananja Putra Pratama, I Wayan Gita Pribadi Santosa Rais Aliffandy Damroni Rebecca Azary Kuncoro Rebecca Azary Kuncoro, Rebecca Azary Regina TC. Tandelilin Regina TC. Tandelilin, Regina TC. Regina Titi Christinawati Tandelilin Rini Maya Puspita Ristini Asih Rizki Amelia Rizki Amelia Rizki Amelia Rizki Fadlilah Santoso, Aloysia Supartinah Sifra Kristina Hartono Singh, Malreen Kaur Harban Siti Fatimah Siti Sunarintyas Sri Widiati, Sri Stefany Elan Saktiyawardani Swastiana Eka Yunita Tri Ayu Hidayani Tri Ayu Hidayani Tri Endra Untara, Tri Endra Urfa Tabtila Virta Devi Kartika Putri Volanda Kusumaningsari Widya Asmara Widya Asmara Yoga, I Gede Krisna Merta Yosaphat Bayu Rosanto Yulita Kristanti