Article Per Year (5 Year)
p-Index From 2021 - 2026
1.235
P-Index
This Author published in this journals
All Journal Jurnal Veteriner Microbiology Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Biomedical Engineering Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease WMJ (Warmadewa Medical Journal) YARSI Medical Journal Biomolecular and Health Science Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology Jurnal Keperawatan Soedirman (The Soedirman Journal of Nursing)
Retno Handajani
Department Of Biochemistry, 00Faculty Of Medicine, Universitas Airlangga, Surabaya
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

Published : 20 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 20 Documents
Search

 1   2 
The frequency of DISC1 Leu607Phe gene polymorphism in schizophrenia patients at Dr. Soetomo General Hospital Surabaya gwenny Ichsan Prabowo; Margarita Maria Maramis; Erikavitri Yulianti; Afrina Zulaikah; Zain Budi Syulthoni; Hendy Muagiri Margono; Retno Handajani
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 1 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.763 KB) | DOI: 10.19106/JMedSci005001201802

Abstract

Schizophrenia is a common health problem in the world, including in Indonesia.Polymorphism of gene disrupted in schizophrenia 1 (DISC1) Leu607Phe is allegedly relatedto the predisposition to schizophrenia. However, studies on the relationship betweenpolymorphism of DISC1 Leu607Phe and schizophrenia in various etnics provided differentresults. The purpose of this study was to determine the frequency of DISC1 Leu607Phegenepolymorphism and its association with treatment response in patients with schizophreniaat Department of Psychiatry, Dr. Soetomo General Hospital Surabaya. In this study, thenumber of male patients with schizophrenia was more than that of the female patients.The mean age of male patients with schizophenia was lower than that of the femalepatients. Schizophrenia patients were primarily came from Javanese ethnic with positiveand negative symptom score (PANSS) lower in male patients than that in the femalepatients. In conclusion, no DISC1 gene polymorphism at codon 607 is observed inschizophrenia patients at Dr. Soetomo General Hospital Surabaya, but the G nucleotidevariation at the number 196.339 in intron regions is found instead.
The Improvement of Community Knowledge, Attitudes and Practices After Covid-19 Socialization Siti Khaerunnisa; Irmi Syafa'ah; Citrawati Dyah Kencono Wungu; Gwenny Ichsan Prabowo; Retno Handajani; Indri Safitri; Harianto Notopuro; Ema Qurnianingsih; Lina Lukitasari; Ira Humairah; Arief Bakhtiar; Suwandito Suwandito; Susi Wahyuning Asih; Zuhrotul Eka Yulis Anggraeni; Ginanjar Sasmito Adi; Ely Rahmatika Nugrahani; Ayesie Natasha Zulka; Soetjipto Soetjipto
Folia Medica Indonesiana Vol. 57 No. 2 (2021): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.653 KB) | DOI: 10.20473/fmi.v57i2.26262

Abstract

This study determined community knowledge, attitudes, and practices after COVID-19 socialization in Rambipuji and Suci Village, Jember District, East Java, Indonesia. This study used the analytic observational design study. As many as 40 people were given socialization about COVID-19 by gathering and online. The questionnaires were completed in two parts included before-socialization and after-socialization. The questionnaire data were analyzed descriptively by calculating frequency, percentage, and inferential statistics by t-test, Spearman correlation, and chi-square test. The data analysis used IBM SPSS version 23 software. The mean and standard deviation of percentage of knowledge pre-test, knowledge post-test, high attitude, moderate attitude, low attitude, very high practice, high practice, sufficient practice, and low practice were 58.33 ± 30.97, 77.70 ± 22.52, 62.5±21.7, 31±22.2, 6.5±17.1, 65±13.8, 32.3±13.8, 1.25±2.4, 1.25±1.8, respectively. Knowledge pre-test and post-test correlated 0.819 (p=0.001) and a t-test with p=0.003. Attitudes and practices correlated with p=0.001. Socialization of COVID-19 was useful to improve the community knowledge, attitudes, and practices in Rambipuji and Suci Village, Jember District, East Java, Indonesia that could prevent the transmission and inhibit the spread of the COVID-19 pandemic in Indonesia. Furthermore, continuous encouragement of COVID-19 socialization in wide areas was recommended.
The Effect of Roasting Temperature on Ferulic Acid Levels of Robusta Coffee Bean with Thin Layer Chromatography (TLC)-Densitometry Sugiyanta Sugiyanta1,2, Harianto Notopuro3 , Jusak Nugraha4 , Soetjipto Soetjipto3 , Retno Handajani
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12168

Abstract

The purpose of this study was to quantitatively analyze ferulic acid levels of Robusta coffee bean extractsfrom two samples from different regions (Argopuro and Kaliwining, Jember District), with differentroasting temperatures (180 °C and 200 °C for 10 minutes) using Thin Layer Chromatography (TLC)-Densitometry method. The analytical method used was the TLC-Densitometry, which utilized methanolsolvent p.a; stationary phase: Merck silica Gel 60 F254 plate; chloroform mobile phase: methanol: formicacid (v/v/v) = (85:15:1) in a saturated chromatographic chamber; the test concentration of 50 ppm and theplates were analyzed with the Densitometry winCATS Camag scanner using a UV-Vis detector at 254 nmwavelength and blue fluorescence at 366 nm wavelength. The results showed that the standard correlationcoefficient (R) of ferulic acid was 0.9947. Quantitative analysis of ferulic acid levels obtained on theaverage results of Argupuro coffee bean extract at roasting temperatures 180 °C and 200 °C amounted to1.935598±0.020671 mg/g and 1.419963±0.051633 mg/g, while Robusta Kaliwining coffee bean extract at180°C and 200°C roasting temperature were 3.18087±0.06738 mg/g and 2.420237±0.1989 mg/g. Statisticalanalysis with One-Way Anova obtained p value <0.005. Least Significant Different Test (LSD) obtainedp value <0.005 in each group. Based on the statistical analysis it was concluded that there was an effectof roasting temperature on ferulic acid levels in Robusta coffee bean extract, with the highest ferulic acidcontent obtained in Robusta Kaliwining coffee bean extract in Jember Regency at roasting temperature of180 °C was 3.180875±0.020671 mg/g.
Antibacterial Activity Test of Red Ginger Extract (Zingiber officinale var. Rubrum) Against Streptococcus pyogenes In vitro Samira Assegaf; Arthur Pohan Kawilarang; Retno Handajani
Biomolecular and Health Science Journal Vol. 3 No. 1 (2020): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v3i1.19130

Abstract

Introduction: Red ginger (Zingiber officinale var. rubrum) is known as an antibacterial agent. Streptococcus pyogenes is the most common bacterial cause of pharyngitis in children and adults. Over the years, antibiotic resistance case is increasing. Therefore, further research of other substances to find an bactericidal or bacteriostatic agent is needed. The aim of this study is to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Z. officinale var. rubrum against S. pyogenes. Methods: Serial dilution test of red ginger extract in Mueller hinton broth (8 concentrations of red ginger extract : 80%, 40%, 20%, 10%, 5%, 2,5%, 1,25%, and 0,625%) and positive control (1 ml bacterial suspension in Mueller hinton broth) was used to determine MIC. MBC was determined by culturing solutions from the previous dilution test into blood agar plate.lResults: MIC of red ginger extract against S. pyogenes could not be determined because the color of red ginger extract affected the turbidity of the dilution test result. MBC of red ginger extract against S. pyogenes was at concentration 20% of red ginger extract.Conclusion: Z. officinale var. rubrum showed antibacterial activity against S. pyogenes.
ANTI-HIV DAN SUBTIPE HIV PADA PASIEN HEMODIALISIS Retno Handajani; Mochammad Thaha; Mochamad Amin; Citrawati Dyah Kencono Wungu; Edhi Rianto; Pranawa Pranawa
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1124

Abstract

Anti-Human Immunodeficiency Virus (Anti-HIV) was performed from 100 plasma Chronic Kidney Disease (CKD) stage 5 patientswith continuous hemodialysis (HD) at the Hemodialysis Instalation Dr Soetomo hospital, Surabaya, Indonesia, using three (3) kind ofreagents: Tri-line HIV Rapid test Device from Acon for HIV 1/2/O as strips form, Foresight HIV 1/2/O Antibody EIA Test Kit from Aconand Anti-HIV 1+2/Subtype O ELISA from Axiom. HIV RNA and HIV subtype were detected by Reverse Transcription Polymerase ChainReaction (RT-PCR) based on HIV gag region and analysis of DNA result. Seventy three % patients were hemodialysed twice in a week andonly 14% with duration more than five (5) years. Most of the patients (43%) were hemodialysed between 100−300 times. From the 100plasma samples was obtained only one (1%) man patient plasma sample with positive anti-HIV. A weak positive of RT-PCR result wasnot succeed to be sequenced for determining the HIV subtype. This cause was suspected due to low levels of HIV RNA in blood. The resultsof this study was expected can be used as an additional management consideration of hemodialysis patients at the Hemodialysis Unit.
The Prevalence and Subtype Distribution of Hepatitis C Virus Infection among Hemodialysis Patients in a Private Hospital in Surabaya, Indonesia MOCHAMAD AMIN; JUNIASTUTI .; TAKAKO UTSUMI; YOSHIHIKO YANO; MOCHAMAD YUSUF; MOCHAMMAD THAHA; PRIYO BUDI PURWONO; RETNO HANDAJANI; SOETJIPTO .; HAK HOTTA; YOSHITAKE HAYASHI; MARIA INGE LUSIDA
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (837.539 KB) | DOI: 10.5454/mi.6.4.5

Abstract

The prevalence of hepatitis C virus (HCV) infection has been as high as approximately 80% in patients with maintenance hemodialysis in public hospitals in Indonesia. However, the prevalence in private hospitals has not been examined yet. The aim of  this study was  to  investigate  the prevalence of anti-HCV antibody and  the subtype distribution in patients with hemodialysis in a private hospital in Surabaya, Indonesia. Sera were obtained from 41 hemodialysis patients in a private hospital in Surabaya. The positive prevalence of anti-HCV antibody was carried out by  the enzyme-linked  immunosorbent assay (ELISA). Anti-HCV-positive sera were subjected  to  reverse transcription-PCR (RT-PCR) to detect HCV RNA and then direct sequencing. The HCV subtype was examined by phylogenetic analysis. Twenty five patients (61%) out of 41 were positive for anti-HCV antibody, and HCV-RNA was detected in 19 patients. The positive prevalence of anti-HCV antibody was 7.7% (one out of 13 patients) among patients who had undergone hemodialysis  for  less  than one year, whereas  it was 85.7%  (24 out of  28  patients)  among  patients who  had  undergone  hemodialysis  for  over  one  year.  Phylogenetic  analysis revealed HCV-1a  (52.6%) was  the most common  subtype,  followed by 1b  (15.8%), 1c  (15.8%), 2a  (5.3%), and 3k (5.3%). In conclusion,  the prevalence of HCV  infection among hemodialysis patients  in a private hospital was as high as  that  in general hospitals. The predominant subtype was HCV-1a, which  is  in accordance with  the previous studies  in general hospitals  in Surabaya,  Indonesia.
Genotype of Hepatitis B Virus Coinfection in Typhoid Patients SUPIANA DIAN NURTJAHYANI; RETNO HANDAJANI
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.254 KB) | DOI: 10.5454/mi.9.3.6

Abstract

Typhoid fever can cause liver disorder and may result in complication. Studies revealed hepatic involvement in thypoid known as typhoid hepatitis. Our previous paper reported the existance of hepatitis B virus (HBV) coinfection in the serum of patients with typhoid using nested-polymerase chain reaction (PCR). Based on sequence divergence, HBV has been classified into 10 genotypes (A-J), which in prediction response and correlates with clinical outcome of chronic HBV infection. This study was conducted to determine the genotypes of HBV in typhoid patients coinfected with HBV in Tuban. Sera obtained from 5 typhoid patients positive HBV by nested PCR). Study was performed by direct sequencing BigDye V1.1 Terminator Cycle Sequencing kit and ABI Prism 310 Genetic Analyzer. Analys had been using the Genetix version 10 software to create the phylogenetic tree. Phylogenetic analysis showed 3 samples as genotype B and 2 two samples as genotype C.
Prevalence of Hepatitis B Virus Infection in Blood Donors Based on Titer Hepatitis B Surface Antigen Examination (HBsAg) SUPIANA DIAN NURTJAHYANI; RETNO HANDAJANI
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.189 KB) | DOI: 10.5454/mi.13.4.5

Abstract

Hepatitis B remains a global public health problem. Infection from hepatitis B virus (HBV) can be transmitted through a blood test or a blood transfusion. This study was conducted to identify the prevalence of HBV infection in blood donors based on examination of HBsAg titers . Blood donors from Tuban Red Cross used as sample. The method used in this research is HBsAg titers examination performed by ELISA according to the procedureoutlined in the Kit. HBsAg titers positive mostly found in men. In men from 13 samples (8.67%) are HBsAg titers positive of 150 samples while in woman all negative for HBsAg titers from 137 samples. The average titer positive was 3.095 with a standard deviation of 0.187. While HBsAg titers negative have average of 0.03 with a standard deviation of 0.14. This study showed that the prevalence of HBV infection in blood donors is most numerous in men with HBsAg titers positive number of 8.67%.
DETECTION OF TUMOR NECROSIS FACTOR- (TNF- ) GENE PROMOTERS POLYMORPHISM AMONG LIVER CIRRHOSIS PATIENTS WITH CHRONIC HEPATITIS B VIRUS (HBV) INFECTION IN SURABAYA, INDONESIA Wungu, Citrawati Dyah Kencono; Amin, Mochamad; Ruslan, S. Eriaty N.; Purwono, Priyo Budi; Kholili, Ulfa; Maimunah, Ummi; Setiawan, Poernomo Boedi; Lusida, Maria Inge; Soetjipto, Soetjipto; Handajani, Retno
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 5 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1302.693 KB) | DOI: 10.20473/ijtid.v7i5.7275

Abstract

Polymorphisms in TNF-α gene promoter region are known of its role in the production of TNF-α which may influences the pathogenesis of liver disease. SNPs in positions 238 and 308 of TNF-α gene promoters may affect the production of these cytokines. This study was aimed to detect Single Nucleotide Polymorphism (SNP) on -238 and -308 positions in the TNF-α gene promoter among liver cirrhosis patients with HBV infection in Surabaya, Indonesia. This was descriptive exploratory research with cross sectional study design using serum liver cirrhosis patients with HBV infection in Endoscopy Outpatient Clinic Dr. Soetomo General Hospital, Surabaya from April-May 2017. SNPs at -238 and -308 on TNF-α gene promoter (rs361525 and rs1800629 respectively) were detected using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with primers specific for the TNF-α promoter region and restriction enzymes NcoI and MspI. The genotypes of TNF-α gene promoter were assessed according to the length of the fragments produced in RFLP. Serum TNF-α levels was measured by commercial ELISA. In this study, as much as 149 positive HBsAg patients was found in Endoscopy Outpatient Clinic, Dr. Soetomo General Hospital, Surabaya. From those amount, as much as 30 liver cirrhosis patients with positive HBsAg were obtained. From 2/30 (6.7%) patients showed the GA heterozygote SNP either position -238 or -308. No patient had the AA genotype. Median blood TNF-α level in women (38 ng / L) was higher than in men (33 ng / L). TNF-α levels in patients with GA heterozygote genotype at -238 and -308 in this research was not different than wild-type (GG genotype). Among patients with liver cirrhosis due to chronic HBV infection in Surabaya, Indonesia, Surabaya, we found GA polymorphisms the TNF-α promoter gene at positions -238 and -308 in 6.7% patients, and did not find homozygous AA polymorphisms. Further studies including larger numbers of patients from various ethnic backgrounds in Indonesia are needed to provide robust data on TNF-α gene promoter polymorphisms and their role in the pathogenesis of liver cirrhosis with HBV infection in this country.
Risk Factor Profile and Role of Cardiovascular Disease Outreach Program by Experts in Rural Communities: A Pilot Study in Magetan Regency, Indonesia Wungu, Citrawati Dyah Kencono; Elvaretta, Clarissa; Palupi, Dian Eka Roro; Qurnianingsih, Ema; Susilo, Hendri; Lukitasari, Lina; Humairah, Ira; Khaerunnisa, Siti; Soetjipto, Soetjipto; Handajani, Retno; Safitri, Indri; Ummah, Fithriyah Cholifatul; Waskito, Langgeng Agung; Prabowo, Gwenny Ichsan; Wiratama, Bayu Satria; Amin, Indah Binti Mohd
Jurnal Keperawatan Soedirman Vol 19 No 2 (2024): Jurnal Keperawatan Soedirman (JKS)
Publisher : Fakultas Ilmu-ilmu Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jks.2024.19.2.10947

Abstract

Cardiovascular outreach programs and risk factors profiling have been regarded as key strategy in preventing and controlling cardiovascular diseases as the leading global causes of death worldwide, especially when being conducted by a professional. This pilot study aims to evaluate the effectiveness of a cardiovascular outreach program in Plaosan District, Magetan Regency, to improve knowledge and influence changes in the community’s behavior related to cardiovascular risk factors. This study was conducted by providing educational interventions and profiling cardiovascular risk factors on 90 adults using consecutive sampling method. Health education was performed by experts and evaluated by pre-post tests before and after the material delivery. Statistical analysis was used to assess the relationship of risk factors that can contribute to cardiovascular disease. This study showed a significant increase in the participants' knowledge score after the intervention (p = 0.007). There were several risk factors for cardiovascular disease in the participants, with the highest risk prevalence being diabetes mellitus (33.33%). Six participants (6.67%) had abnormal electrocardiography results. In conclusion, our study showed a high prevalence of cardiovascular risk factors in rural communities and the importance of cardiovascular outreach programs by experts to improve the understanding of cardiovascular disease in a specific community.
Co-Authors A Kentjono, Widodo Afrina Zulaikah Amin, Indah Binti Mohd Ayesie Natasha Zulka B Notopuro, Paulus Bakhtiar, Arief Bayu Satria Wiratama David S. Perdanakusuma Edhi Rianto Elvaretta, Clarissa Ely Rahmatika Nugrahani Fis Citra Ariyanto Gatut Suhendro Ginanjar Sasmito Adi HAK HOTTA Harianto Notopuro Hendri Susilo Hendy Muagiri Margono Henydhar Bramastivira Mahdani Hernomo Ontoseno Kusumobroto Humairah, Ira I Gusti Agung Komang Diafari Djuni Hartawan Indri Safitri Indri Safitri, Indri Juniastuti Juniastuti Kawilarang, Arthur Pohan Khaerunnisa, Siti Luki Indriaswati Lukitasari, Lina Margarita Maramis, Margarita MARIA INGE LUSIDA Maria Inge Lusida MOCHAMAD AMIN MOCHAMAD AMIN Mochamad Amin Mochamad Yusuf Alsagaff Mochammad Amin Mochammad Thaha MOCHAMMAD THAHA Nurwasis Nurwasis Nyilo Purnami Palupi, Dian Eka Roro Poernomo Boedi Setiawan Prabowo, Gwenny Ichsan Pranawa Pranawa Priyo Budi Purwono Qurnianingsih, Ema Radias Dwi Padmani Rizaliyana, Sitti Ruby Riana A Ruby Riana Asparini Ruslan, S. Eriaty N. S. Perdanakusuma Samira Assegaf Siti Khaerunnisa SOETJIPTO SOETJIPTO . SOETJIPTO . Soetjipto Soetjipto Sulistyo Mulyo Agustini Supiana Dian Nurtjahyani Susi Wahyuning Asih Suwandito Suwandito Syafa’ah, Irmi TAKAKO UTSUMI Ulfa Kholili Ummah, Fithriyah Cholifatul Ummi Maimunah Waskito, Langgeng Agung Wiwik Misaco Yuniarti Wungu, Citrawati Dyah Kencono YOSHIHIKO YANO YOSHITAKE HAYASHI Yulianti, Erikavitri Zain Budi Syulthoni Zuhrotul Eka Yulis Anggraeni