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Nining Handayani
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Journal : Jurnal Veteriner

 1 
Vitrifikasi Blastosis Mencit dengan Metode Kriolupv ?O I Wayan Batan; I Ketut Suatha; Wahono Esti PrasetyoningtyaserB; Nining Handayani; Ita Djuwita; Arief Boediono
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cryopreservation is an ultra rapid freezing process to preserve tissue or organ. The studywas conducted to identify the effectiveness of cryoloop vitrification method and the viability ofembryos following vitrification. Embryos at blastocyst stage were vitrified by placing them inequilibration medium containing 10% ethylene glycol (EG) and phosphate buffer saline (PBS) wichsupplemented with 20% new born calf serum for 8-10 minutes. The blastocysts were then removedand put in vitrification medium (15% dimethyl sulfoxide, 15% EG, and 0.5M sucrose), and theprocess in the vitrifivcation medium not longer than 25-30 seconds. The blastocysts were immediatelytransferred to the vitrification medium film in the cryoloop and plunged into 100 ml liquid nitrogen.The warming process was done by immersing the cryoloop which carried the vitrified blastocstsinto PBS supplemented with 20% serum and 0.5M sucrose for 1 minute, and then removed to samesolution supplemented with 0.25M sucrose and 0.1M sucrose for 2 minutes respectively. Theblastocysts were washed 4 times in kalium simplex optimized medium (KSOM) and cultured indrops of KSOM in 5% CO2 incubator at 370C. The observations were done every 6 hours for 48hours using inverted microscope ( Olympus IX70 Japan). The viability of embryos was assessed onthe basis of the intact morphology, reexpansion of the blastosul, and the development of embryosinto advance stage. The results showed that 85.71% of vitrified embryos, developed into advancestages and 19% of them hatched. In conclusion the cryoloop can be used to vitrify the embryos.
Co-Authors A. Rohendi Acep Rohendi Achmad Dheni Suwardhani Aisyah Fitri, Nurul Amanah Amanah Angelica Riadi Alim Saputro Angelina Riadi Alim Saputro Anisa Qadarsi Ananda Hamsah Anom Bowo Laksono Apriani, Diah Arief Boediono Arini Estetia Putri Aurelia Demtari Tuah Ayunda Nazli, Selsi Bayu Wahyudi Budiarti, Tresna Clara Riski Amanda Dasrun Hidayat Dasti P, Saparingga Edenita, Helga Irene Efawati, Yen Elly Yanah Arwanih Ellyana, Ellyana Endang Komara Erliany Syaodih Erliany Syaodih Fani Pangabdian, Fani Fatimah, Jardinia Dian Feby Bantoyot Fitaloka, Neng Diana Fitra, Eldeast Jane Abdul Fitriana, Liskanita Nur Gartika Rahmasari, Gartika Habibah, Zahrotul Handayani, Ni Luh Hera Wahyuni Hidayat, Fanny Agustina Hudzaifah Al Fatih I Ketut Suatha I Wayan Batan Ignatius Wiseto P Agung Iis Iskandar, Iis Ita Djuwita Jujur, Utang Khariri Khariri Kusuma, Kevin Rizki Agung M Noor, Chairil Manggiasih Dwiayu Larasati Mardiyanto Mardiyanto Mega Putri Utami Melva Louisa Mery Tania Mochtar, Fransiska Nadya, Putie Neni Nuraeni, Neni Nerissa Arviana, Nerissa Noer Rizky Helga Widowati Nurrahma U, Asrina Nurwansyah, Atikah Nurwijayanti Pamungkas, Tegar Paramita Deniswara Poppy Putri Pratiwi Pratama, Sony Andik Pratama, Willy Danis Pratiwi, Putie Nadya Purwadhi Purwadhi Purwadhi Rahardjo, Franciska Rahmawati, Gartika Rebecca Noerjani Angka Reski Mulia Paramita Rian Andriani, Rian Rinawati Rinawati Rizki Adriansyah Rubini Rohendi, A Rosidawati Wiradirja, Imas Saparingga Dasti Putri Saraswati, Yuni Shafilla Yunilma Andriany Siti Chanifah Sukajie, Bambang Sutisnawati, Yayah Syafei, M. Yani Taufiq, Josefa Vanina Winata Thapjun, Aujchara Ungke Antonjaya Veranita, Mira Vorachat, Chonchanok Wahono Esti PrasetyoningtyaserB Wiryo, Purwadhi Sawal Yani Restiani Widjaja, Yani Restiani Yuliarni, Indah Ziraluo, Eprianto