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Prolonged-heated High-Fat Diet Increase the Serum LDL Cholesterol Level and Induce the Early Atherosclerotic Plaque Development in Wistar Rats Valentina Yurina; Ema Pristi Yunita; Tri Yudani Mardining Raras; Achmad Rudijanto; Kusworini Handono
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rats are one of the most widely used animal models in health research. However, since rats are relatively resistant to atherosclerosis, the transgenic rats often used to study atherosclerosis in rats. Our study suggests a prolonged-heated lipid to induce atherosclerosis in rats, therefore provide a more low-cost option to study atherosclerosis in rats. Aims of this study is to compare the effect of heated high-fat diet (HFD) to the LDL and HDL cholesterol level and foam cell formation in the Wistar rat animal model. Rats were divided into three groups, control group was given with the normal diet, and the other two treated groups received oxidized HFD (heated HFD) and HFD, respectively. The heated HFD contain lard that was previously heated at 190°C for 24 h. Diet was given for 8 weeks. The serum LDL and HDL cholesterol level were measured before and 4th and 8th weeks after treatment with help of colorimetric measurement. The aorta analyzed for the foam cell formation after HE staining using the light microscope.  The prolonged- heated HFD significantly increased the LDL cholesterol from the 4th week of the treatment (p = 0.023). The rats aortic from HFD and heated HFD treated groups showed a mononuclear cells infiltration and the early foam cell formation. The heating process of the lipid caused lipid oxidation which significantly increased the LDL cholesterol level of the animal model and foam cells formation. This study suggests that Wistar rats with heated HFD could demonstrated early atherosclerosis plaque development.
Exploring Systemic Lupus Erythematosus Pathogenesis through Animal Models: A Systematic Review of Humanized and Pristane-Induced Lupus Mice Airlangga, Dimas Ikhsan; Rahmawati, Hanifa Rizky; Susianti, Hani; Handono, Kusworini
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19434

Abstract

Studies involving experimental animals to explore the pathogenesis of systemic lupus erythematosus (SLE) which leads to the selection of optimal therapy have been widely conducted. The well-known model used to study SLE includes the pristane-induced mouse model and the more recently developed humanized mouse model that implants human immune cells into immunodeficient mice. The current state of the research has yet to provide a systematic review that analyzes both model and its contribution to our understanding of SLE pathogenesis. This systematic review-based study aims to provide a comprehensive overview of the development and application of pristane-induced and humanized mouse models. We obtained several relevant article sources include: (1) Search Strategy, on databases such as PubMed, MEDLINE, ScienceDirect, and Cochrane by adjusting the protocols listed in the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA); (2) Eligibility based on exclusion and inclusion criteria; and (3) Data Extraction. The findings show that 30 articles are relevant to the subject matter. Several strains of mice were used in the model of the 0.5 pristane injection method and the humanized mice model. All studies showed similar patterns in the onset and manifestation of SLE in mice models with slight variations. The purpose of using the pristane injection method and humanized mice model is adjusted to the output of each study. A variety of research preferences can be used as a reason for choosing pristane and humanized cells transplanted SLE methods in making lupus model mice.
HUBUNGAN ANTARA PENANDA sCD28 DAN sCTLA-4 TERHADAP PERSENTASE SEL LIMFOSIT T CD8+CD45RA+ PADA PENUAAN IMUN: - Handono, Kusworini; Susanti, Hani; Pratama, Mirza Zaka; Kalim, Handono; Wahono, Caesarius Singgih; Ria Famuji, Siti Roziah; Firdaningrum, Nimas Eka; Sumarta, Norma Hanifah; Rizky Fachry, Ade Wlidan
Majalah Kesehatan Vol. 10 No. 4 (2023): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/majalahkesehatan.2023.010.04.1

Abstract

Penuaan merupakan proses alami dan kompleks yang ditandai dengan penurunan fungsi fisiologis manusia sehingga berakibat pada perubahan sistem imun. Sampai saat ini, deteksi penuaan imun (immunosenescence) masih memiliki kendala yang berarti. Penanda satu-satunya yang diyakini sebagai prediktor penuaan imun adalah immune risk profile (IRP). Namun, IRP hanya dapat diamati dengan pemeriksaan flowsitometri yang membutuhkan alat canggih dan keterampilan khusus. Oleh karena itu, diperlukan penelitian yang menemukan suatu penanda deteksi penuaan imun melalui metode yang lebih mudah. Beberapa marker kostimulator memiliki bentuk terlarut di dalam serum. Penanda CD28 dan CTLA-4 pada membran memainkan peran utama pada regulasi aktivasi limfosit T dan marker diagnostik pada autoimun ataupun penuaan imun. Meskipun demikian, bentuk terlarut dari penanda tersebut masih belum banyak diteliti.  Tujuan dari penelitian ini adalah meneliti keterkaitan antara penanda sCD28 dan sCTLA-4 terhadap persentase IRP sel limfosit T CD8+CD45RA+ pada penuaan imun. Molekul kostimulator sCD28 dan sCTLA4 diukur menggunakan ELISA, sedangkan persentase sel limfosit T CD8+CD45RA+ menggunakan flowsitometri. Pada penelitian ini didapatkan 87 responden terdiri dari 23,0% responden kelompok individu muda, 40,2% kelompok pasien Lupus Eritematosus Sistemik (LES), dan 36,8% responden kelompok lansia. Kadar sCD28 dan sCTLA-4 terhadap persentase sel limfosit T CD8+CD45RA+ memiliki hubungan yang tidak signifikan pada penuaan imun. Pada penuaan imun, fungsi sel limfosit T yang mengekspresikan CD45RA menampilkan cacat proliferatif dan pensinyalan, tetapi tetap polifungsional.
Possible Role of Akt in Mossy Fiber Sprouting: Akt Activity and CA3 Mossy Fiber Sprouting in a Kainate Model of Epilepsy: Akt Activity and CA3 Mossy Fiber Sprouting in Epilepsy husna, Machlusil; Handono, Kusworini; Sujuti, Hidayat; Aulanni'am, Aulanni'am
Journal of Tropical Life Science Vol. 14 No. 3 (2024): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.09

Abstract

The most prevalent pathological phenomenon observed in patients with epilepsy is hippocampal mossy fiber sprouting (MFS), which is thought to be associated with epileptic progression, such as worsening seizure control, cognitive function, and behavior. MFS is discovered in the dentate gyrus and the hippocampal Cornu Ammon 3 (CA3) area. The CA3 area is involved in memory, so disturbances in that area can affect memory impairment in patients with epilepsy. The mammalian target of rapamycin complex-1 (mTORC1) is also associated with MFS. Akt is an upstream activator of mTORC1 and a downstream target of mammalian target of rapamycin complex-2 (mTORC2) and plays a role in cytoskeleton organization. We analyzed Akt activity and MFS in the CA3 zone in an in vitro model of kainate-induced epilepsy. We divided organotypic hippocampal slice cultures (OHSCs) into a kainate (epilepsy) group and a control (untreated) group. On the 10th day in vitro (DIV), the kainate group was exposed to 8 µM kainic acid for 48 h, diluted in the medium. At 32 DIV, we measured Akt activity through western blotting and CA3 MFS through synaptoporin fluorescence intensity observed using confocal laser scanning microscopy. We found that Akt activity increased significantly (p = 0.000) in the kainate group, and the synaptoporin fluorescence intensity also increased in the stratum oriens of the CA3 area (p = 0.049) in the kainate group. Our findings implied that Akt may play a role in MFS development. Because Akt is a main downstream target of mTORC2, mTORC2 may also be involved in MFS development. Further research is required to clarify these findings.
Vitamin D3 induces stem cell activation via Lgr5-Bmi1 expression and improving mouse colitis histology index Wibowo, Satrio; Pramadhani, Almira; Subandiyah, Krisni; Handono, Kusworini; Poeranto, Sri
Narra J Vol. 3 No. 3 (2023): December 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i3.430

Abstract

Conventional therapy for inflammatory bowel disease using long-term anti-inflammatory drugs does not seem to provide optimal results. Adjuvant therapy using vitamin D3 is believed to have an essential role in repairing the colonic mucosa through the activation of colonic stem cells. The aim of this study was to demonstrate the effect of vitamin D3 in mucosal repair through stem cell activation, marked by leucin-rich repeat-containing G protein-coupled receptor 5 (Lgr5) and B lymphoma Mo-MLV insertion region 1 (Bmi1) expression and decrease the mouse colitis histology index (MCHI) score. In this study, 50 Mus musculus strain BALB/c were divided into five groups: negative control group, colitis group, and colitis groups with vitamin D3 administration of 0.2 mcg, 0.4 mcg, and 0.6 mcg per 25 g body weight for seven days. Dextran sulfate sodium (DSS) 5% was used to induce colitis. Lgr5-Bmi1 expression was measured using immunodoublestain fluorescent labeling method. Our data suggested that administration of vitamin D3 significantly increased expression of Lgr5-Bmi1 in the colonic mucosa. The colitis group treated with the highest dose of vitamin D3 (0.6 mcg/25 gram) showed the lowest MCHI score (3.60±0.64) while the lowest dose of vitamin D3 had the highest MCHI score (12.60±1.47). In conclusion, stimulating stem cells, vitamin D3 administration stimulates mucosal regeneration, as demonstrated by upregulated expression of Lgr5-Bmi-1.
Strengthening Moral and Material Support for Lupus Patients and Their Families at the Kupu Parahita Indonesia Foundation Through Cinematherapy Handono, Kusworini; Dewi, Elvira Sari; Desfryda, Elynca Putri; Dwinadella, Sephia; Putri, Choirinnisa Meilia Ayu; Kalim, Handono; Wahono, Cesarius Singgih; Barlianto, Wisnu; Erawati, Dini Rachma; Pratama, Mirza Zaka; Sari, Tita Luthfia; Nurdiana, Nurdiana; Kusuma, Zaenal
Journal of Innovation and Applied Technology Vol 9, No 2 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2023.9.2.13

Abstract

Lupus patients and their families require adequate support to manage the disease's progression. This community service aims to help the Kupu Parahita Indonesia Foundation provide strengthened moral and material support for lupus patients and their families at the Kupu Parahita Indonesia Foundation. Results: The cinematherapy planned by the PKNM and Community Service Team received much positive feedback from patients and families. The sharing session expressed that patients and their families are grateful for the opportunity to enjoy free recreation together. Enthusiasm comes with enthusiasm to meet people with fellow lupus. . In conclusion, cinematherapy can help the Kupu Parahita Indonesia Foundation provide lupus patients and their families with more substantial moral and material support.
Negative Effect of Cigarette Smoke: Black Garlic Opportunities for Prevention of Ovulation Disorders Asih, Sari Wulan; Nurseta, Tatit; Handono, Kusworini; Yudhanto, Hendy Setyo
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.585

Abstract

Women's issues are primarily related to infertility. This study aimed to demonstrate that ovarian healing in wistar rats exposed to cigarette smoke was impacted by the administration of extracted black garlic. This research uses True Experimental methods in vivo in the laboratory to identify the cause and effect of the variables being tested. This method involves replication, randomization, and control. The design chosen was a post-test-only control group design, where the experimental group received treatment and the control group did not, without random selection. The results of the study showed that there was no effect due to exposure to cigarette smoke with an increase in cortisol and a decrease in the number of secondary follicles in the ovaries of female wistar rats as well as the administration of Black Garlic (Allium sativum) extract. Black garlic extract unaffected cortisol levels and the number of secondary follicles or repair of the ovaries due to exposure to cigarette smoke. Further research is needed in dosing black garlic extract or combining it with other ingredients to provide effective results.
Optimalisasi Dukungan Sosial Bagi Pasien Lupus dan Keluarga Pasca Pandemi Covid-19 di Yayasan Kupu Parahita Indonesia Dewi, Elvira Sari; Putri, Choirinnisa Meilia Ayu; Desfryda, Elynca Putri; Dwinadella, Sephia; Wahono, Cesarius Singgih; Sari, Dewi Purnama; Handono, Kusworini; Kalim, Handono
International Journal of Community Service Learning Vol. 7 No. 2 (2023): May 2023
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v7i2.56434

Abstract

Selama pandemi Covid-19, pasien lupus dan keluarga dihadapkan dengan berbagai stresor dan tantangan yang dapat mempengaruhi dukungan sosial. Kegiatan pengabdian pada masyarakat ini bertujuan untuk mengoptimalkan dukungan sosial bagi pasien lupus dan keluarga pasca pandemi Covid-19 di Yayasan Kupu Parahita Indonesia. Metode yang digunakan dalam kegiatan pengabdian pada masyarakat ini adalah dengan melakukan Forum Group Discussion (FGD) untuk menggali kebutuhan pasien lupus dan keluarga akan dukungan sosial; menyelenggarakan pertemuan offline antara pasien lupus, keluarga, dan tenaga kesehatan pemerhati lupus; serta memberikan edukasi intens secara online. Hasil kegiatan menunjukkan bahwa dari kegiatan FGD, didapatkan permasalahan bahwa belum semua pasien lupus dan keluarga di Yayasan Kupu Parahita Indonesia mengenal dan memahami lupus dengan baik. Melalui kegiatan yang diselenggarakan secara offline maupun online, masalah yang didapat saat FGD teratasi, di mana pasien lupus dan keluarga lebih memahami lupus dan dengan sharing sesama pejuang lupus, kebutuhan dukungan sosial pasien lupus dan keluarga terpenuhi. Dapat disimpulkan bahwa pendekatan secara intens baik secara offline maupun online sangat diperlukan untuk mengoptimalkan dukungan sosial bagi pasien lupus dan keluarga.
Possible Role of Akt in Mossy Fiber Sprouting: Akt Activity and CA3 Mossy Fiber Sprouting in a Kainate Model of Epilepsy: Akt Activity and CA3 Mossy Fiber Sprouting in Epilepsy husna, Machlusil; Handono, Kusworini; Sujuti, Hidayat; Aulanni'am, Aulanni'am
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.09

Abstract

The most prevalent pathological phenomenon observed in patients with epilepsy is hippocampal mossy fiber sprouting (MFS), which is thought to be associated with epileptic progression, such as worsening seizure control, cognitive function, and behavior. MFS is discovered in the dentate gyrus and the hippocampal Cornu Ammon 3 (CA3) area. The CA3 area is involved in memory, so disturbances in that area can affect memory impairment in patients with epilepsy. The mammalian target of rapamycin complex-1 (mTORC1) is also associated with MFS. Akt is an upstream activator of mTORC1 and a downstream target of mammalian target of rapamycin complex-2 (mTORC2) and plays a role in cytoskeleton organization. We analyzed Akt activity and MFS in the CA3 zone in an in vitro model of kainate-induced epilepsy. We divided organotypic hippocampal slice cultures (OHSCs) into a kainate (epilepsy) group and a control (untreated) group. On the 10th day in vitro (DIV), the kainate group was exposed to 8 µM kainic acid for 48 h, diluted in the medium. At 32 DIV, we measured Akt activity through western blotting and CA3 MFS through synaptoporin fluorescence intensity observed using confocal laser scanning microscopy. We found that Akt activity increased significantly (p = 0.000) in the kainate group, and the synaptoporin fluorescence intensity also increased in the stratum oriens of the CA3 area (p = 0.049) in the kainate group. Our findings implied that Akt may play a role in MFS development. Because Akt is a main downstream target of mTORC2, mTORC2 may also be involved in MFS development. Further research is required to clarify these findings.
Association of clinical manifestations, disease activity, and medications on premature atherosclerosis in systemic lupus erythematosus Pratama, Mirza Zaka; Kusworini Handono; Cesarius Singgih Wahono; Ahmad Bayhaqi Nasir Aslam; Syahrul Chilmi
Heart Science Journal Vol. 6 No. 1 (2025): Challenges in Managing Acute Heart Failure
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.hsj.2025.006.01.11

Abstract

Background SLE is distinguished by the development of multiple autoantibodies that lead to chronic inflammation and increased risk of cardiovascular diseases, especially atherosclerosis. Objective This examination sought to explore the association between the disease activity, clinical manifestations, and medication with the atherosclerotic lesion from SLE patients. Methods This inquiry investigated forty-two female SLE patients (18-45 years old) who met the 2019 EULAR/ACR assortment decency with matched healthy individuals as control A cross-sectional study was sanctioned at the Rheumatology Clinic of Saiful Anwar General Hospital Malang between July and November 2023. Demographic data, clinical manifestations, and medication history was documented in the medical records. Disease activity was stelled by the SLEDAI-2K score. Carotid Intima-Media Thickness (cIMT) and Flow-Mediated Dilation (FMD) (after brachial BP cuff inflation up to 200 mmHg for five minutes) examinations were used as atherosclerosis marker. Results Markedly higher of cIMT mean was demonstrated in SLE patients compared to healthy individual (0.51±0.11 vs 0.40±0.11 mm, p<0.001). FMD was subtancially curtailed in SLE patients set side to side to control (0.00 (0.00-0.10) vs 0.10 (0.00-0.28) mm, p=0.022). There was no statistical distinctness of the cIMT and FMD among SLE patients according to the presence of clinical manifestations. Neither cIMT nor FMD showed a statistically correlated with the disease activity. Patients who received hydroxychloroquine (0.57±0.02 vs. 0.50±0.09 mm, p=0.043) and cyclophosphamide (0.55±0.09 vs. 0.48 ± 0.10 mm, p=0.031) had higher cIMT. Higher cIMT was demonstrated in subjects who received steroid (p=0.045) and the dosage of steroid was essentially enforced to cIMT (R=0.418, p=0.034). Conclusion Our study unveil that early atherosclerosis was evidenced in patients with SLE and several medications might affect the progressivity of atherosclerosis.
Co-Authors Abd. Rasyid Syamsuri Abdurrachman Abdurrachman Achmad Rifa’i, Achmad Achmad Rudijanto Achmad Rudijanto Agustina T Endharti, Agustina T Agustina Tri E Agustina Tri Endharti Ahmad Bayhaqi Nasir Aslam Airlangga, Dimas Ikhsan Albaar, Thoha M. Alfandy, Tommy N. Asih, Sari Wulan Ati Rastini Atma Gunawan Atma Gunawan Atma Gunawan Aulanni'am, Aulanni'am Balindra, Fredlina Rossa Bambang Rahardjo Benny A Pradana, Benny A Bowo Hery Prasetyo, Bowo Hery BP Putra Suryana BP Putra Suryana Cesarius Singgih Wahono Dalhar, Mochamad Daniel Happy Putra Dantara, Tri W.I. Dany Farida Desfryda, Elynca Putri Dessy Setiawati Dessy Wulandari, Dessy desy wulandari Devi, Nabila Sinta Dewi Purnama Sari Dewi, Elvira S. Dhani, Fauzan K. Dhian E. P. Harnandari Diadjeng Setya Wardani Dian Ayu Wulansari, Dian Ayu Dian Hasanah Dian Sukma Hanggara Dimas Ikhsan Airlangga Djoko W. Soeatmadji Dona Marisa, Dona Dwinadella, Sephia Eko, Mudjiwijono Handaru Elvira Sari Dewi Ema Pristi Yunita Ema Pristi Yunita, Ema Pristi Engli, Katherina Enny Listyawati Erawati, Dini Rachma Erna Amin Faisal Faisal Farida ** Fatchiyah Fatchiyah Firdaningrum, Nimas Eka Gizta, Aura Bella Handono Kalim Hani Susanti, Hani Hani Susianti Hanik Ruliani Haribowo, A S Hidayat Sujuti HMS Chandra Kusuma I Putu A.S Ibrahim Njoto Ihda Dian Kusuma Indah Jayani Ivan A Hartono, Ivan A Janasti, Laksmitha Kedaton, Rafi Andyah Arum Kedaton, Rafi’ Andyah Arum Krisni Subandiyah Kurnianingsih, Nia L Enggar Fitri Laksmi Karunia Tanuwijaya Laksmi Karunia Tanuwijaya Lisyani Suromo Loeki Enggar Fitri M Rasjad Indra Machlusil Husna, Machlusil Maghfirah, Halimi Bidaimi Marsetyawan HNE Soesatyo Muhammad Anshory, Muhammad Muhammad Masyhur Nelly Ismayasih Nurdiana Nurdiana Nurhadi, Pradana Perdana Aditya Rahman Poetri, Levrita Nindya Pramadhani, Almira Prasetyo, Dwi A. Pratama, Mirza Z. Pratama, Mirza Zaka Purnomo, Athaya F. Purwanto Adipireno Putri, Choirinnisa Meilia Ayu Putu A. Herliawati R.I R.I Radhitio A Nugroho, Radhitio A Rahmawati, Hanifa Rizky Ria Famuji, Siti Roziah Rifa’i, A Rizky Fachry, Ade Wlidan Rossy Meilani Rulli Rosandi Safrina Dewi Ratnaningrum, Safrina Dewi Sari, Riana Trinovita Sari, Tita Luthfia Satrio Wibowo Singgih Wahono Solly Aryza Sri Andarini Indreswari Sri Poeranto, Sri Subandi Subandi Subandi Subandi Suharyo Hadisaputro Sulistomo, Hikmawan Wahyu Sulistyorini Sulistyorini Sumarno . Sumarta, Norma Hanifah Suryana, Bagus Putu Putra Sutrisno Sutrisno Suwito, Mat Syahrul Chilmi Syahrul Chilmi, Syahrul Tatit Nurseta Tatit Nurseta Torajasa Achamar Tri Yudani Mardining Raras VALENTINA YURINA VALENTINA YURINA Wahono, Caesarius Singgih Wahono, Cesarius S. Wahono, Cesarius Singgih Wisnu Barlianto Wisnu Barlianto Wisnu Barlianto Yahya Irwanto Yona One Sidarta, Yona One Yudhanto, Hendy Setyo Yuliana Salman Zaenal Kusuma