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IDENTIFIKASI BAKTERI Staphylococcus aureus PADA PREPUTIUM DAN VAGINA KUDA (Equus caballus) (Identification of Staphylococcus aureus in Preputium and Vagina of Horses (Equus caballus)) Yulina Rahmi; Darmawi Darmawi; Mahdi Abrar; Faisal Jamin; Fakhrurrazi Fakhrurrazi; Yudha Fahrimal
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3805

Abstract

This research aims to identify the Staphylococcus aureus (S. aureus) bacteria from preputium and vagina of horses. Five swabs preputium and 5 swabs vagina samples from 3-9 years olds horse in the village of Bebangka, Gelelungi, Reloop, and Kelapeng, Pegasing Aceh Tengah District were used in this study. Preputium and vagina swabs of horses were dipped into reaction tube containing Nutrient Broth (NB). Staphylococcus aureus was identified on manitol salt agar (MSA). Separated colony colored bright yellow on the media were tested for Gram staining, hemolysis, catalase, and sugar test (glucose and manitol). The result showed that the isolates growth on MSA were colored bright yellow, Gram positive, β hemolisis, and catalase positive. The isolates fermented manitol and glucose. Isolates identified from horses preputium and vagina showed the characteristic of S. aureus. In conclusion S. aureus bacteria were positively identified from all of swabs samples, both in preputium and vagina of horses.Key words: Staphylococcus aureus, horse, preputium, vagina
11. Diagnostic surra (Trypanosoma evansi) on Buffalo Slaughtered in Banda Aceh and Aceh Besar Slaughterhouses Based on Card Agglutination Test for Trypanosoma evansi (CATT) Farida Athaillah; kartika Amira; Yudha Fahrimal
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.10164

Abstract

This study aims to detect the presence of parasites indirectly by looking at antibodies to Trypanosoma evansi by using the method of Card Agglutination Test for Trypanosoma evansi (CATT). A total of 50 heads of buffaloes cut at Banda Aceh and Aceh Besar Slaughterhouses were taken blood for examination at the Parasitology and Research Laboratory of the Faculty of Veterinary Medicine of Syiah Kuala University. The principle of the CATT method is to look at the bonds between antibodies and antigens. Positive results are characterized by the formation of agglutination on the CATT paperboard. The score of agglutination reactions is divided into 5 categories, positive triple (+++) indicates agglutination is very strong, which means the possibility of newly infected animals, positive two (++) signifies strong agglutination, which means the animal may have been infected long enough, one (+) denotes moderate agglutination, meaning the animal has already been infected with T. evansi, dubious (±) denotes weak or dubious agglutination and, negative (-) signifies no agglutination. The results showed that from 50 samples examined, 14% (7/50) positive samples of agglutination were very strong, 24% (12/50) positive samples of strong agglutination, 48% (24/50) positive samples of moderate agglutination, and 0 negative samples T. evansi. The CATT method is very sensitive because 86% of the 50 buffaloes are tested positive or infected with T. evansi.
1. Diversity of Parasites on the Garing Fish (Tor tambroides) in Jorong Ikan Banyak River, Gunuang Omeh Subdistrict, Lima Puluh Kota Regency, West Sumatera Province Zulhamezi Nofila; Yudha Fahrimal; Razali Daud
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.9933

Abstract

The study aims to determine diversity of parasites that infect garing fish and determine their prevalence. A total of 50 garing fish samples were taken from three places, 17 fishes from the forbidden pool of Lubuk Sati, 16 fishes from the forbidden pool of Batu Subalah and 17 fishes from the forbidden pool of Lubuk Pinang along the Jorong Ikan Banyak River, Gunuang Omeh Subdistricts, Lima Puluh Kota Regency West Sumatra Province. Garing fish sample were examined in the parasitology laboratory of Bukittinggi Veterinary institute for examination of ectoparasites and endoparasites, using the natif method. The result research found that five ectoparasites that infect the garing fish is Dactylogyrus sp. was found on the gills with 38.1% prevalence, Gyrodactylus sp. was found in fins and mucus with a prevalence of 14%, Ichtyopthirius multifiliis was found in gill, mucus and fins with 36.7% prevalence, Trichodina sp. was found in the fins and mucus with a prevalence of 5.9%, Argulus sp. found on fins and mucus with a prevalence of  7.% and there are two endoparasites that infect the garing fish they are Camallanus sp. in the intestine with a prevalence of 7.9%  and Capillaria sp. in intestine with  prevalence of 10.1%.
TINGKAT PARASITEMIA MENCIT PUTIH (Mus musculus L.) JANTAN GALUR DDY DIINFEKSI Trypanosoma evansi ISOLAT PIDIE DAN PEMALANG (Parasitemia Level of Male White Mice (Mus musculus L.) DDY Strain Infected with Trypanosoma evansi of Pidie and Pemalang Isolate) Febiola Rama Sari; Yudha Fahrimal; Ummu Balqis; Aprilia Wardana; Muhammad Hambal
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3947

Abstract

This study aimed to observe parasitemia of DDY white mice infected with T. evansi of Pidie and Pemalang isolates obtained from Balai Besar Penelitian Veteriner (BBPV) Bogor. Twelve DDY mice were divided into 3 groups, each group consisted of 4 mice. Mice in the first group were not treated with any treatment. The second group was inoculated with 104 T. evansi Pidie isolate and the third group was inoculated with 104 T. evansi of Pemalang isolate. Mice blood were collected every two days from tail vein for parasitemia examination. Parasitemia examination was conducted until all mice die. The results indicate that there was difference in parasitemia level between the two isolates. Parasitemia of mice infected with Pidie isolate characterized by rapid rise of parasitemia in blood (107-108/mL of blood) in a short time (2-4 days) since first parasitemia was detected and followed by death at day 4. While the parasitemia of mice infected with Pemalang isolate increased in the blood (108-109/mL of blood) on day 4 and maintained for a few more days and then fluctuated for a few more days before the animal died.Key words: Trypanosoma evansi, parasitemia, Pidie isolate, Pemalang isolate
Parasitemia of Male White Mice (Mus musculus L.) DDY Strain Infected with Trypanosoma evansi Pidie and Pemalang Isolate Febiola Rama Sari; Yudha Fahrimal; Ummu Balqis; Didik T Subekti; Aprilia Wardana
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i2.3317

Abstract

This study aimed to find out the parasitemia of DDY white mice infected with T. evansi of Pidie and Pemalang isolates obtained from Bbalitvet Bogor. A total of 12 mice were divided into 3 treatment groups. Group 1 (K1) without any treatment, group 2 (K2) was inoculated with 104 T. evansi Pidie isolate, and group 3 (K3) was inoculated with 104 T. evansi Pemalang isolate. Parasitemia examination was carried out every two days and the level of parasitemia was observed as well. Parasitemia examination was conducted until all mice died. Parasitemia of mice infected with Pidie isolate was characterized by rapid rise of parasitemia in blood (107-108/mL of blood) in a short time (2-4 days) since first parasitemia was detected and followed by death at day 4. The parasitemia of mice infected with Pemalang isolate increased in the blood (108-109/mL of blood) on day 4 and maintained for a few more days and then fluctuated for a few more days before the animal was dead. In conclusion, there was the difference in parasitemia level between Pidie and Pemalang isolates. Key words: Trypanosoma evansi, parasitemia, Pidie isolate, Pemalang isolate
5. Hematological Changes in Rattus Norvegicus infected with Trypanosoma evansi after Administration of Ethanol Extract of Phyllanthus Emblica Dina Maulidya Siregar; Nuzul Asmilia; Yudha Fahrimal; Abdullah Hamzah; M. Hasan; Razali Daud; M. Isa; Dasrul Dasrul; Cut Nila Thasmi; Priyo Sambodo
Jurnal Medika Veterinaria Vol 15, No 2 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i2.23917

Abstract

This research aims to determine the value of erythrocyte, hemoglobin, and hematocrit of white rats (Rattus norvegicus) infected with Trypanosoma evansi and given ethanol extract of Malacca leaves (Phyllanthus emblica). The animals used in this study were 20 male rats. All rats were randomly divided into 5 treatment groups, each group consisting of 4 rats. The negative control group (K0) without T. evansi infection and without malacca leaf ethanol extract, the positive control group (K1) was infected with T. evansi without giving malacca leaf ethanol extract, the treatment group K2, K3, K4 were infected with T.evansi and were given ethanol extract Malacca leaf with doses of 300mg/kg BW, 600mg/kg BW, and 900mg/kg BW, respectively. The extract was given orally for 3 consecutive days. On the fourth day, blood was drawn for all groups through the orbital vein with a hematocrit pipette after administration of 0,2 ml Ketamin. Then the erythrocyte, hemoglobin, and hematocrit values were calculated using a hematology analyzer. The mean (± SD) erythrocyte values (106 / µl) were 7.66 ± 0.10, respectively; 2.65 ± 0.24; 4.56 ± 0.91; 6.58 ± 0.10; 5.86 ± 0.27; hemoglobin (g / dl) were 13.64 ± 0.26, respectively; 4.67 ± 0.13; 8.38 ± 0.72; 11.25 ± 0.58; 9.55 ± 0.56, the hematocrit (%) were 40.97 ± 0.67, respectively; 28.27 ± 0.76; 30.44 ± 0.48; 37.70 ± 0.40; 34.06 ± 0.17. The results showed that administration of ethanol extract of malacca leaves at a dose of 600mg/kg BB had a significant effect in maintaining the value of erythrocytes, hemoglobin, and hematocrit of white rats that had been infected with Trypanosoma evansi.
PERKEMBANGAN DAN GAMBARAN ANATOMIS LARVA INFEKTIF (L3) Haemonchus contortus YANG DIBIAKKAN DENGAN VERMICULLITE (Development and Anatomical Description of Infektive Larvae (L3) Haemonchus contortus Cultured with Vermicullite) Muhammad Hambal; Rahmi Sulastri Mukhtar; Muhammad Hanafiah; Yudha Fahrimal; Winaruddin Winaruddin; Zakiah Heryawati Manaf
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i1.4043

Abstract

This study was aimed at obtaining basic information about bioecology of Haemonchus contortus, related to larval development and anatomical description of infective larvae (L3). In this study, the ova of Haemonchus contortus were harvested out of the intestines of goats, those were slaughtered in municipal slaughterhouse in Kampung Jawa Banda Aceh. The ova of Haemonchus contortus were then cultured using vermiculite as media, and observed daily until the larvae reached 3rd stage larvae. The result revealed that the larvae need 7 days to reach from eggs into infective larvae. Larvae obtained has uniform morphology, with a body diameter of 22 μm, tail length up to 55 μm hyaline, esophagus posterior to the tail of 592 μm, length of esophagus anterior to posterior esophagus 145 μm, and a total body length of larvae 737 μm.
PENGARUH PEMBERIAN EKSTRAK KULIT BATANG JALOH TERHADAP GAMBARAN MIKROSKOPIS GINJAL TIKUS PUTIH (Rattus novergicus) YANG DIINFEKSI (Trypanosoma evansi The Effect of Wilow Bark Ekstract Administration on Microscopic Feature of Male Rats (Rattus novergicus) Kidney Infected with Trypanosoma evansi) Nurdiniyah Nurdiniyah; Nazaruddin Nazaruddin; Sugito Sugito; M Nur Salim; Yudha Fahrimal; Siti Aisyah
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3949

Abstract

The aims of this study was to examine the ability of willow bark extract to prevent kidney tissue damage from T. evansi infection. This study used 25 rats which were randomly divided into 5 treatment groups, each groups consisted of 5 rats. Rats in control group (K0) were not infected with T. evansi and without administration of willow bark extract. Group 1 (P1) was infected with 103/0,3 ml of T. evansi without willow bark extract administration. Group 2, 3, and 4 were infected with 103/0.3 ml T. evansi and received willow bark extract at dose of 30, 45, and 60 mg/kg bw, respectively. Willow bark extract were administered for 3 days consecutively. On day 4, the rats were sacrificed using chloroform and the kidneys were collected for histopathological examination. Data were analyzed descriptively. The results showed that the administration of willow bark extract at doses of 30 and 45 mg/kg bw were able to prevent damage of tubule cells and glomerular renal, while the administration of 60 mg/kg bw resulted in kidney damage, indicated by necrosis in tubular glomerular cells. It could be concluded that willow bark extract at doses of 30 and 45 mg/kg bw were effective to prevent tissue damage in kidneys.Key words: kidneys, willow, Trypanosoma evansi
23. IDENTIFIKASI PARASIT PADA IKAN LELE DUMBO (Clarias gariepinus) DI DESA LAMBRO DEYAH KECAMATAN KUTA BARO KABUPATEN ACEH BESAR Identification of Parasites in Catfish (Clarias gariepinus) in Lambro Deyah Kuta Baro Aceh Besar Adistya Putra Rizki; Yudha Fahrimal; Razali Daud; T. Fadrial Karmil; Muhammad Hambal; Zuhrawati Z
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i2.4390

Abstract

The study aimed at identifying the types of parasites that infect catfish as well as to determine the prevalence of the parasite species. A total of 30 catfish were collected using nets then examined at Parasitology Laboratory, Veterinary Medicine Faculty, Syiah Kuala University Banda Aceh. Ectoparasites examination was carried out by directly observed the outer surface of fish body and by mucus smear examination microscopically. While endoparasites examination was done by observation on the inner surface of the digestive tract. The study revealed that five types of parasites infected catfish were Dactylogyrus (43.33%), Gyrodactylus (20%), Tricodina (3.33%), Camallanus sp (3.33%), and unidentified worm (3.33%). ____________________________________________________________________________________________________________________ Key words: catfish, ectoparasite, endoparasite
Pengaruh Sirih (Areca catechu) Sebagai Intelmentika Terhadap Nematode Gastrointesninal Pada Kambing Yudha Fahrimal
Jurnal Agripet Vol 3, No 1 (2002): Volume 3, No. 1, April 2002
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v3i1.3817

Abstract

ABSTRACT.The efficacy and effective dose of bettlenut (Areca catechu) powder against gastrointestinal nematode worms was studied. Twenty five local goats at the age of 1 1.5 years of age weighing 8 to 12 kg were used in this study. All goat were naturally infected with gastrointestinal nematodes and they all had fecal egg count 3000/gram. The goats were randomly divided into 5 group: group I as a non treated control and group I, II, III, IV, and V were given bettlenut powder 5 gram, 10, 15 and 20 gram, respectively. The nematode worm were identified and grouped into family of Trichostrongylidae (Trichostrongylus sp. and Haemonchus sp.) and strongylidae (Bunostomum sp., Oesophagostomum sp. and Strongylus sp.). in general, bettlenut powder in all doses is very effective against gastrointestinal nematode worms from both families compare to control group (P 0.01) and no significant different among administered doses (P 0.05). Bettlenut powder in all doses given is vey effective (P 0.01) against Trichostrongylus sp. and Haemonchus sp. from family of Trichostrongylidae. Bettlenut powder in all doses given is also very effective (P 0.01) against Bunostomum sp., Oesophagustomum sp. and effective (P 0.05) against Strongylus sp. from family of Strongylidae. The dose of 5 gram of bettlenut powder per goat is recommended for treating goat invested with gastrointestinal nematode worms because it is saver and can be given more than once.