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2. Characterization Of Lactic Acid Bacteria (LAB) Origin Sumatran Orangutan (Pongo abelii) In Zoo Bukittinggi Based On Analysis 16 S rRNA Siti Hajar; Safika Safika; Darmawi Darmawi; Wenny Novita Sari; Erdiansyah Rahmi; Yandi Syahputra; Nurliana Nurliana; Rinidar Rinidar
The International Journal of Tropical Veterinary and Biomedical Research Vol 1, No 2 (2016): Vol. 1 (2) November 2016
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.484 KB) | DOI: 10.21157/ijtvbr.v1i2.5944

Abstract

The purpose of this study was to characterize the bacterial 16S rRNA gene of Sumatran orangutan (Pongo abelii) Bukittinggi West Sumatera zoo. The sample used in this study are lactic acid bacteria of the Sumatran orangutan (Pongo abelii) derived from zoo Bukittinggi West Sumatra Indonesia. This study was an exploratory study that conducted at the Laboratory through several stages. The first stage was the isolation of LAB from faeces of Sumatran orangutans using MRS agar medium and then cultured in a liquid medium NB. The next stage was the isolation of total DNA, and then, the third stage was the amplification of the 16S rRNA gene and agarose gel electrophoresis. Then,in the fourth stage of determining the DNA sequence and analysis of DNA sequence homology. The final stage was the computational analysis of 16S rRNA gene Sumatran orangutan (Pongo abelii). The results showed that lactic acid bacteria (LAB) from the Sumatran orangutan (Pongo abelii) is close to lactic acid bacteria Lactobacillus helveticus strain IMAU50151 with the levelof similarity of 89%. It is possible that these bacteria is a new species or the species that LAB has not been reported in Genbank.
Study of Knowledge, Attitude and Action of Buffalo Breeders in the Villages Around Nagan Raya Power Plant on the Impact of Fly Ash Exposure Ira Susanti; Rinidar Rinidar; Sugito Sugito
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 2 (2018): Vol. 3 (2) November 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.422 KB) | DOI: 10.21157/ijtvbr.v3i2.12334

Abstract

One of the biggest contributors of air pollution is the production of coal fly ash from power plant industry. Fly ash has an immediate impact on human and animal health around the power plant. Many buffaloes in Nagan Raya usually grazing near the 110-megawatt steam power plant that using low rank coal. This action raises concerns about buffalo’s health due to exposure to fly ash. This study aimed to examine the relationship of knowledge, attitude and action of buffalo breeders to the impact of fly ash exposure on the buffaloes. This research used analytical survey with cross sectional design. This study was conducted in February 2018 with 30 respondents from four villages around Nagan Raya Power Plant to evaluate their knowledge, attitudes and actions by using questionnaires. The data were analyzed statistically with Chi Square Test with SPSS for Windows version 17.0. The results showed that there was no significant correlation between the knowledge of buffalo breeders and the impact of fly ash exposure (P 0.05), while the attitude and actions of buffalo breeders had a significant correlation with the impact of fly ash exposure (P 0.05). These results concluded that the buffalo breeders in the villages around the power plant did not realize the impact of fly ash arising from coal of power plant. It is expected that the associated institution would conduct a counseling about the impact of fly ash exposure as well as good management of buffaloes.
The Effect Of Cellulase Content On Phytoestrogens Formation (Genistein) Materials On Fermented Soybean Waste Aspergillus niger Vara Tassa Sutari; Nurliana Nurliana; Sugito Sugito; Darmawi Darmawi; Rinidar Rinidar
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.805 KB) | DOI: 10.21157/ijtvbr.v3i1.11362

Abstract

The activity of the cellulase enzyme and the content of phytoestrogen (genistein) forming is determined during the fermentation of soybean waste with Aspergullus niger. The analysis is performed at fermentation incubation time of 0, 48, 96, 144 and 192 hours and treatment with addition of micro nutrient (V2) and without micro nutrient (V1). Data analysis on cellulase enzyme activity on phytoestrogen forming material is performed using T test. The average of cellulase content of fermented soybean waste increases from 0.032-0.139 IU / ml (V1) and 0.061-0.158 IU / ml (V2) after 48 hours into 0.238 IU / ml (V1) and 0.245 IU / ml (V2) after 192 hours The average of genestein levels in the fermented soybean waste increases as well from 0.101 - 0.573 mg / g (V1) and 0.114 - 0.587 mg / g (V2) after 48 hours and increase into 0.722 mg / g (V1) and 0.699 mg / g (V2) after 192 hours. Statistical analysis indicates that the treatment of V1 and V2 has an effect on the increase of cellulase enzyme content and genistein level (P 0.05). The correlation between enzyme activity of cellulase and genistein on soybean waste fermented with Aspergillus niger is significant (R2 = 0,949). The relationship between the enzyme performance and its formation material is running synergistic, cellulase enzyme activity and genistein level experience significant increase during the fermentation process.
15. Potency of Methanolic Extract of Sernai Stems (Wedelia biflora) as Analgesic on Mice (Mus musculus) Annisa Rizka Rahmatia; Rinidar Rinidar; T. Armansyah TR; Rosmaidar Rosmaidar; Abdul Harris; Yudha Fahrimal
Jurnal Medika Veterinaria Vol 12, No 2 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i2.4230

Abstract

The purpose of this research was to determine the potency of methanolic extract of sernai stem (Wedelia bilflora) as analgesic to mice (Mus muculus) compared to ibuprofen. This research use split-plot method with 5 treatments and 5 replications. Group P0 as negative control was given aquadest, P1 as positive control was given ibuprofen, while P2, P3, and P4 were given the extract with dosage of 40, 50, and 60 mg/kg bw, respectively. The pain analysis of mice was conducted using hotplate method and the observation was carried out at 0 minute, 30 minutes, 60 minutes, 90 minutes, and 120 minutes. The data was analyzed using analysis of varians (Anova) followed by Duncan’s test. The results of this research showed  that P1, P2, P3 and P4 had significant difference (P0,05) from P0. P1 did not have significant  difference (P0,05) from P2 but it was different from P3 and P4 (P0,05). From this result we can conclude that methanolic extract of sernai stem was potential as analgesic, dosage of 40 mg/kg bw was equal to ibuprofen, while 50 mg/kg bw and 60 mg/kg bw showed better effect than ibuprofen.
4. Study of Knowledge, Attitude and Action of Buffalo Ranchers in the VillagesAround Nagan Raya Power Plant on the Impact of Fly Ash Exposure Ira Susanti; Rinidar Rinidar; Sugito Sugito
Jurnal Medika Veterinaria Vol 14, No 1 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i1.11158

Abstract

One of the biggest contributors to air pollution is the production of coal fly-ash from the power plant industry. Fly-ash has an immediate impact on human and animal health around the power plant. Many buffaloes are maintained by Nagan Raya people and are often consumed by the community. The buffalo ranchers in this area release their livestock around the 110 MW Steam Power Plant that using low-rank coal. This ranchers’s behavior raises concerns about buffalo health due to exposure to fly ash. This study aims to examine the relationship between knowledge, attitude, and action of buffalo ranchers to the impact of fly ash exposure on the buffalo they maintain. This research used a cross-sectional design. The research time was done in February 2018 by taking 30 samples as respondents in four villages around Nagan Raya Power Plant. The tools used to assess knowledge, attitudes, and actions were using questionnaires. Data were analyzed statistically with the Chi-Square Test through SPSS for Windows version 17.0. The results showed that there was no significant relationship between the knowledge of buffalo ranchers and the impact of fly-ash exposure (P 0.05) while the attitude and actions of buffalo ranchers had a significant relationship with the impact of fly-ash exposure (P 0.05, P 0 , 05). These results conclude that the buffalo ranchers in the villages around the plant have not understood the impact of fly-ash arising from coal in the Nagan Raya power plant. It is expected that the relevant parties to conduct counseling about the impact of fly-ash exposure and the way of good buffalo management.
8. The Total of Leukocytes Mice (Mus musculus) Exposed To Secondhand Smoke Extract And Given Watermelon (Citrullus vulgaris) Dewi Mauliza; Rusli Rusli; Roslizawaty Roslizawaty; Rosmaidar Rosmaidar; Rinidar Rinidar; Dian Masyitha
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i1.4269

Abstract

This study aimed to determine the effect of the red watermelon extract can affect the number of leukocytes in mice exposed to cigarette smoke. This study was designed with a completely randomized design using a unidirectional pattern 20 male mice (Mus muscullus) were divided into 4 groups. K1 negative controls were given 0.5 ml of distilled water, K2 positive control by exposure to cigarette smoke and 0.5 ml of distilled water, K3 by exposure to cigarette smoke and red watermelon extract were given a dose of 22 mg / mouse and K4 by exposure to cigarette smoke and were given extracts of red watermelon a dose of 44 mg / mouse. Exposure to cigarette smoke and red watermelon extract conducted for 30 days, making blood on day 31. Blood sampling performed at Plexus Retroorbitalis. Furthermore, counting the number of leukocytes. Data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. The results of this study showed that the extract of watermelon for 30 days showed their real influence (P 0.05) to the number of leukocytes in mice. It is concluded that free radicals contained in cigarette can cause a leukocytosis, an inflammatory, oxidative stress and red watermelon extract can minimize cell damage.
4. The Value of Erythrocytes, Hemoglobin, and Hematocrit of Mice (Mus musculus) Exposed to Cigarette Smoke and Given Red Watermelon Extract (Citrullus vulgaris) Yola Heryanita; Rusli Rusli; Rosmaidar Rosmaidar; Zuraidawati Zuraidawati; Rinidar Rinidar; Nuzul Asmilia; Muhammad Jalaluddin
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i1.4106

Abstract

The aim of this research was to investigate the effect of red watermelon (Citrullus vulgaris) extract to the value of erythrocytes, hemoglobin, and hematocrit of mice (Mus muscullus) exposed to cigarette smoke. This study followed direct complete randomized design by using 12 males’ mice which divided into 4 groups. The treatment group is divided by the negative control group which given 0,5 ml of aquadest, the positive control group which exposed to cigarette smoke and 0,5 ml of aquadest. The treatment group I was exposed to cigarette smoke and given red watermelon extract dose 22 mg/Bw mice. The treatment group II was exposed to cigarette smoke and given red with watermelon extract dose 44 mg/Bw mice. The exposure to cigarette smoke and the given of red watermelon extract were conducted for 30 days. The blood taking was performed on day 31 in the Plexus retroorbital.  Furthermore, the number of erythrocytes, hemoglobin and hematocrit were calculated. The data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. The results of this study showed that the extract of red watermelon for 30 days showed a highly significant effect (P 0.01) to the number of erythrocytes, hemoglobin, and hematocrit of mice. It is concluded that free radicals contained in the cigarette can cause a hemoglobin desaturation, increased blood viscosity, oxidative stress and red watermelon extract can minimize the damage.
14. The Potency of Sernai (Wedelia biflora) Leaf N-hexan Extract as Analgesic Compared to Ibuprofen on Mice (Mus musculus) Zulkarnaini Zulkarnaini; Rinidar Rinidar; Rosmaidar Rosmaidar; T. Armansyah TR; Abdul Harris; Muhammad Isa
Jurnal Medika Veterinaria Vol 12, No 2 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i2.4144

Abstract

This study aimed to determine the analgesic potential of sernai leaves n-Hexan extract on 24 male mice, aged 2-3 month with 20-25 g weighed. This study used split-plot design consisted of 6 groups. P0 as negative control was given aquades and P1 as positive control was given ibuprofen (10 mg/kg Body Weight). Treatment groups including P2, P3, P4, and P5 were given n-Hexan extract of sernai leaves with dosage of 15, 30, 45, and 60 mg/kgBW, respectively. Pain assessment was conducted using Hot Plate method. Observation of mice behaviour including licking it's front and back leg, or by jumping. This study showed there was significant difference (P0,05) between dosage and time. Duncan’s test showed P1 was significantly different (P0,05) from P2, but P3 and P5 were not significantly different from P1. P3 and P4 were significantly different (P0,05) from P2 and not significantly different from P1, while P5 was not significantly different from P3 and significantly different from P1. We can conclude that dosage of 30 and 45 mg/kgBW of n-Hexan extract of sernai leaves had analgesic potential on mice, and 60 mg/kgBW was comparable to ibuprofen as pain relieve in mice
16. The Number Of Leukocyte And Leukocyte Differential In Broilers That Infected With Eimeria tenella And Given Neem Leaf Extract And Jaloh Extract Rizki Aulia; Sugito Sugito; M. Hasan; T. Fadrial Karmil; Gholib Gholib; Rinidar Rinidar
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i2.4667

Abstract

The aim of this research was to determine the effect of giving neem leaf extract combined with jaloh towards the total of leukocytes and differential leukocyte in broilers that was infected with Eimeria tenella. This research samples were 20 Cobb stain broilers 14 days old. Design the research was complete randomized design with five treatments and each treatment consists of four repetitions. First treatment (P1) as negative control which was only given mineral water; second treatment (P2) as positive control was is given 5 mg/L anti-stress commercial in drinking water; Third treatment (P3) was given 1000 mg/L Jaloh extract; Fourth treatment (P4)was given 250mg/L neem extract; and fifth treatment (P5)was given 1000mg/L jaloh extract combined with 250mg/L neem extract. Anti-stress commercial and extract treatment dissolved in drinking water. The treatment start from 08.00 until 18.00 for ten days. Next in the 11th day (chicken 25 days old)was inoculated Eimeria tanella sporulatif as much as 1 x 104 ookista/ml orally. Then the blood was taken sampling when the chicken 30 days old (five days after infected). Blood sampling was done in the brachial vein. Furthermore, the number of leukocytes and differential leukocyte.Was calculated data were analyzed using complete randomized design with the help of SPSS for Windows 1.8 program. The result of this research showed that giving jaloh and neem leaf extract were not giving significant effect (P0,05) towards the number of leukocyte as well as differential leukocyte of broilers that was infected with Eimeria tenella. The conclusion of this research were giving jaloh leaf extract and neem for 10 days was not giving significant effect towards number of leukocytes and differential leukocyte broilers that infected with Eimeria tenella