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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Prosiding Seminar Biologi Jurnal Akuntansi dan Pajak Inovasi : Jurnal Ekonomi, Keuangan, dan Manajemen AFEBI Management and Business Review JURNAL MANAJEMEN INDUSTRI DAN LOGISTIK Owner : Riset dan Jurnal Akuntansi Journal on Education JURNAL MANAJEMEN BISNIS Jurnal Ilmiah Edunomika (JIE) Jurnal Informasi dan Teknologi Jurnal Ekobis : Ekonomi Bisnis & Manajemen Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Jurnal Ekonomi dan Bisnis Terapan Quantitative Economics and Management Studies Jurnal Studi Islam Lintas Negara (Journal of Cross-Border Islamic Studies) Jurnal Studi Ekonomi dan Bisnis Islam (SEBI) Daengku: Journal of Humanities and Social Sciences Innovation Jurnal Ekonomi Jurnal Kolaboratif Sains Journal of Social And Economics Research Jurnal Ilmu Pendidikan dan Sosial International Journal of Management and Business Economics Journal of Economic Education and Entrepreneurship Studies Indonesian Journal Of Business And Entrepreneurship Research Nusantara Journal of Multidisciplinary Science Jurnal Pengabdian Masyarakat dan Riset Pendidikan Jurnal Intelek Dan Cendikiawan Nusantara Jurnal Intelek Insan Cendikia International Journal of Social and Education (INJOSEDU) International Journal of Economics, Accounting, and Management journal of social and economic research JAS (Jurnal Akuntansi Syariah) Journal of Digital Business Innovation Atestasi : Jurnal Ilmiah Akuntansi
Irsyad Kamal
Universitas Padjadjaran
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Optimizing DNA Extraction Methods from Leaf and Wood Tissues to Support Dipterocarp Conservation and Sustainable Forest Management Arrofaha, Nawwall; Rachmat, Henti Hendalastuti; Dwiyanti, Fifi Gus; Adinugroho, Wahyu Catur; Siregar, Iskandar Zulkarnaen; Kamal, Irsyad; Syaputra, Dhika; Salamah, Andi
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 16 No 1 (2026): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.16.1.13

Abstract

Dipterocarpaceae are economically important, contributing over 85% of Indonesia's timber exports. However, this crucial resource is increasingly threatened by illegal logging, habitat destruction, and the illegal timber trade, which jeopardize dipterocarp population. Furthermore, conservation efforts utilizing genetic and forensic techniques often encounter substantial challenges due to the complexities in DNA extraction protocol. To address this, the study aimed to enhance the efficiency of DNA extraction methodologies by comparing two methods: the modified cetyltrimethylammonium bromide (CTAB) and the Genomic DNA Mini Kit (Plant) from Geneaid Biotech Ltd. The research focused on leaf and wood samples from two species, spesifically Rubroshorea leprosula (Miq.) P.S.Ashton & J.Heck and Shorea laevis Ridl. For each of these species, five leaf and five wood samples were extracted using both methods. The quality of the DNA extraction was evaluated using electrophoresis and quantified with a Qubit fluorometer. Higher DNA concentrations were obtained with the modified CTAB method compared to the GeneAid kit for both R. leprosula and S. laevis, particularly in leaf tissue. The GeneAid kit consistently exhibited low DNA yield efficiency compared to the modified CTAB method for both species. Additionally, PCR amplification of both leaf and wood samples confirmed that the extracted DNA was suitable formolecular analyses. These findings not only contributed to laboratory applications but also served as practical tools for species identification and genetic conservation for sustainable forest management and law enforcement.
Co-Authors A. Malik Fajar Abdul Rosid Adhadian Akbar Adinugroho, Wahyu Catur Adzka Rosa Sanjayyana Agung Anggara Rudini Agus Santoso Amalis Karisma Amaliya Shofiana Andi Salamah Andreas Recki Prasetyo Anindita Nirkin Maheswa Aprih Santoso Arida Susilowati Iswanto Arif Rahman Ariyanto M Arrofaha, Nawwall Asyifa Anandya Athallah, Muhammad Ariq Baiq Farhatul Wahidah Bastanta Prawira Tarigan Cakranegara, Pandu Adi Carmeline, Benedicta Christina Cattleya Rejito Chitra Indah Sari Choeriyah, Siti CNAWP, Rizal Perlambang Cuk Jaka Purwanggono Darmawanto Darmawanto Deru R Indika (Universitas Padjadjaran - Indonesia) Desty Hapsari Kirana DEWI SARTIKA Dj, Arifhan Ady Etty Susilowati Farhany Richas Fathimah Azzahra Rahadian Fifi Gus Dwiyanti Fitria Sari Hapsari Widayani Hasan Basri Hety Devita ISKANDAR ZULKARNAEN SIREGAR Iwan Setiawan Jamaludin, Endang Johnny Chandra Joval Ifghaniyafi Farras Judijanto, Loso Komalasari, Ika Kurnia Khafidhatur Rafiah Kurnia Khafidhatur Rafiah KUSUMADEWI SRI YULITA Latifa Mischa Saqina Layyinaturrobaniyah Lukman Hakim M. Aqshel Revinzky M. Chairul Ihsan Matthew Olufemi Adio Medeilia Bernike Br Ginting Miradji, Moh. Afrizal Miswari Miswari Muhamad Aqshel Revinzky (Universitas Padjadjaran - Indonesia) Muhammad Aqshel Revinzky Muhammad Aqshel Revinzky Nizar Muhammad Hisyam Nadhir Muhammad Rispan Affandi Muhammad Rizki Perdana Nur Hayati Pandu Adi Cakranegara Prihastuti, Asepma Hygi Putra, Agus Rachmat, Henti Hendalastuti Rahlana Shidiq Azqar Ratih Pratiwi Rini Armin Riri Cornellia Rizkiati Khasanah Rizky Ramadhan Rosari, Renya Safaruddin Saipul Al Sukri Salim Diarra Siska Yuli Anita Sonia Sischa Eka Putri Sulwani Husna Afrizal Sunu Widianto Supriyanto Susila, Susila Susilo Surahman Syaputra, Dhika Tuti Dharmawati Tuti Dharmawati Ubbadul Adzkiya' Yudi Azis Zet Ena