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Total plate count dalam isi telur ayam ras di pasar tradisional Kecamatan Waru, Kabupaten Sidoarjo Budiarto, Budiarto; Kirana, Sabila Citra; Lestari, Tita Damayanti; Budiarto; Puspitasari, Yulianna; Mutamsari Witaningrum, Adiana; Permatasari, Dian Ayu
Current Biomedicine Vol. 1 No. 2 (2023): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.2.86-94

Abstract

Background: Microbial contamination in consumed chicken eggs can endanger human health. Bacteria can grow and develop in food so that it can reduce the food quality. Determination of food quality can be done by various methods, one of which is Total Plate Count (TPC). Objective: The aim of this study was to determine the level of bacterial contamination in chicken eggs sold at traditional markets in Waru District, Sidoarjo Regency. Methods: Thirty samples were collected from two traditional markets, Waru Market and Wadung Asri Market. This study used the pour plate method of TPC test. Egg contents were diluted and then inoculated in Nutrient Agar. The media inoculated with the sample suspension was incubated at 37°C for 24 hours. The growing bacterial colonies were counted and analyzed using the Standard Plate Count. This study's data are presented descriptively and compared with the Indonesian National Standard (SNI) 7388-2009 maximum requirement of contaminant bacteria in chicken eggs is 1×105 CFU/mL. Results: The results showed that the averages TPC in two traditional markets were 2.1×104 CFU/mL in Waru Market and 3.4×103 CFU/mL in Wadung Asri Market. The average TPC still meets the maximum limit of microbial contamination listed in SNI 7388-2009. Conclusion: The average values of TPC in both markets are not the same, but the qualities of chicken eggs sold in Waru Market and Wadung Asri Market are good and suitable for consumption.
Prevalence and Infestation of Ectoparasite in Dabung Ducks in Some Districts in Bangkalan Madura Prawasa, Ramadhiniyanti Putri Alif; Nunuk Dyah Retno Lastuti; Dadik Raharjo; Poedji Hastutiek; Endang Suprihati; Dian Ayu Permatasari
Journal of Parasite Science Vol. 7 No. 2 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i2.40134

Abstract

Identify the type of ectoparasite prevalence and infestation pattern of ectoparasite on Dabung duck in Bangkalan Madura regency. This research conducted from February to April 2022 with sample of 55 tails and identified in parasitology laboratory of the Veterinary Parasitology Division, Faculty of Veterinary Medicine, Airlangga University, Surabaya with the permanent mounting method without coloring. This research was a descriptive research, study survey, and cross sectional study analytical. The type of ectoparasite that attacked Dabung duck in Geger subdistrict, Dabung village and Galis subdistrict, Pekaan Dajah village were Menacanthus stramineus, Menopon gallinae, Lipeurus caponis with a prevalence of 100% from a sample 55 tails of Dabung duck. The analysis results of infestation pattern of ectoparasite infestation on Dabung ducks used correspondence text on the body regions of Dabung ducks in Geger subdistrict, Dabung village showed that M. stramineus ticks on chest region, M. gallinae in head neck region and L. caponis in wing region. Burneh subdistrict, Burneh village founded M. stramineus ticks in abdomen region, M. gallinae in head-neck region, and L. caponis in wing region. Galis subdistrict, Pakaan Dajah village showed that M stramineus lice in head-neck region, L. caponis in wing region and M. gallinae in spine region.
Prevalence Rate and Infection Degree of Helminthiasis on Pigeon (Columbia Livia Domestica) in North Surabaya Ihda Hanny, Khurun'In Fadia; Djoko Legowo; Mufasirin; Kusnoto; Dian Ayu Permatasari; Poedji Hastutiek
Journal of Parasite Science Vol. 7 No. 2 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i2.48823

Abstract

Pigeon meat is an alternative option to other poultry meat such as chikens. As pigeons are easy to keep and quickly reproduce. Improper hygene practices is a strong factor in helminthiasis transmission. This study aims to know the prevalence and degree of infection of helminthiasis in North Surabaya. 70 samples were taken from pigeon butchers in North Surabaya from September to November 2022. Dissection method was used for prevalence rate count and modified McMaster method was used to count degree of infection. The result shown that 70% of samples had positive worm infection. Types of worms found were R. cesticillus (55.7%), Ascaridia sp. (25.7%), Capillaria sp. (14.2%), Echinostoma sp. (2.8%) and Heterakis sp. (1.4%). Qualitative exam shown helminthiasis was more prevalent in adult pigeon than in squab, but analysis with Chi-square test shown no significant association between helminthiasis infection and age of the pigeons (P>0.05). Quantitative exam with McMaster method shown degree of infection of single Ascaridia infection in adult pigeons is 340 EPG while in Capillaria sp. is 287.5 EPG and 150 EPG in Heterakis. All of them are considered mild infection. Thus, proper loft and feed hygene method should be informed to prevent more transmission.
Prevalence and Degree Trematoda Infection in Dairy Cattle Friesian Holstein at Koperasi Usaha Tani Ternak Suka Makmur Pasuruan Rohmah, Miftahur; Sunarso, Agus; Hermadi, Herry Agoes; Hastutiek, Poedji; Permatasari, Dian Ayu; Kusnoto, Kusnoto
Journal of Parasite Science Vol. 8 No. 2 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i2.60450

Abstract

Trematode worm infections in dairy cattle have not been widely reported in various regions in Indonesia. This study aims to determine the prevalence and degree of trematode worm infection in Friesian Holstein dairy cattle at KUTT Suka Makmur, Pasuruan. The research design used survey study and cross-sectional study. The study was conducted in July-September 2023. The number of samples taken was 100, which were selected from proportional random sampling of four sub-districts, namely Grati, Nguling, Lekok, and Lumbang, with the age ranges under a year, 1-2 years, and above 2 years. The sample were examined using the sedimentation method and Flukenfinder® method. Positive samples were further examined using the McMaster method to determine the number of worm eggs per gram of feces. The results of this study obtained a prevalence of 54% with low and moderate degrees of infection. The trematode worm species found through fecal examination were Fasciola gigantica and Paramphistomum cervi. The results of chi square statistical analysis showed that sample examination method and age were related to the prevalence of trematode worms, yet location was not related to the prevalence of trematode worms. The results of kruskal wallis statistical analysis showed that age and location influence the degree of trematode worm infection.
Identification, Prevalence, and Degree of Digestive Tract Protozoa Infection in Dairy Cows at KUTT Suka Makmur Grati Pasuruan Margaretha, Josephira Intan; Hastutiek, Poedji; Permatasari, Dian Ayu; Sunarso, Agus; Rahardjo, Dadik; Suwanti, Lucia Tri
Journal of Parasite Science Vol. 8 No. 2 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i2.60454

Abstract

The aim of this research is to identify, determine prevalence, and degree infection of protozoa gastrointestinal tract in dairy cow at KUTT Suka Makmur, Pasuruan district. The research was conducted from July until September 2023 by taking 100 dairy cow faeces sample from 4 villages of KUTT Suka Makmur. The samples were examined by sedimentation and floating methods. Species are identified by looking at morphology of protozoa gastrointestinal. There were of 85 (85%) samples positively infected with protozoan. There were 3 species that infect the digestive tract, those are Eimeria sp., Balantidium sp., and Blastocystis sp. The highest infection of protozoa in this research was Balantidium sp. (54%) followed by Eimeria sp. (47%). In this study, we found that 54 samples (54%) infected by one kind of protozoa and 31 samples (31%) infected by mixed protozoa. The result of Chi Square showed no significant difference in the age on the prevalence of digestive tract protozoa. The result of Kruskal Wallis showed no significant difference in the age and degree infection of Eimeria sp.
Prevalence and Infection’s Degree of Gastrointestinal Nematode Worm in Friesian Holstein Dairy Cows at KUTT Suka Makmur Pasuruan Regency Utami, Kinanti Putri; Santoso, Kuncoro Puguh; Suwanti, Lucia Tri; Hastutiek, Poedji; Permatasari, Dian Ayu; Sunarso, Agus
Journal of Parasite Science Vol. 8 No. 2 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i2.60504

Abstract

This study aims to determine the type, prevalence, and infection’s degree of gastrointestinal Nematode worm in Friesian Holstein Dairy Cows at KUTT Suka Makmur, Pasuruan Regency. The study was conducted in July – September 2023. One hundred of feces samples were collected from four districts at KUTT Suka Makmur working area. Samples were examined by sedimentation and floating methods. Positive samples were further examined by McMaster methods to determine the number of worm eggs per gram feces. The type of worms were Oesophagostomum sp. (24%), Haemonchus sp. (20%), Mecistocirrus sp. (13%), Trichuris sp. (8%), Ostertagia sp. (6%), Trichostrongylus sp. (6%), Nematodirus sp. (3%), and Strongyloides sp. (3%). The prevalence of gastrointestinal Nematode worm was 66% with mild and severe degrees of infection. The Chi Square test results showed that age had no effect on the prevalence and infection’s degree of gastrointestinal Nematode worm in Friesian Holstein dairy cows at KUTT Suka Makmur. While location had effect on the prevalence but had no effect on the infection’s degree.
The Quality of Milk Production in Friesian Holstein (FH) Dairy Cattle Experiencing Repeat Breeding at KUD Tani Wilis Sendang, Tulungagung Regency Sintya Kumalasari Wibowo; Aldin Akbar Rahmatullah; Cindy Ercha Aulia Putri; Pudji Srianto; Dian Ayu Permatasari; Dadik Raharjo; Rimayanti; Erma Safitri; Mohammad Auzaie Afandi; Nuurin Ajrin Karim
Journal of Applied Veterinary Science And Technology Vol. 5 No. 2 (2024): October 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I2.2024.135-139

Abstract

Background: Repeat breeding is a notable reproductive problem where cows do not conceive after several insemination attempts, and it is typically defined as three or more unsuccessful attempts. This results in reduced reproductive efficiency, lower economic sustainability of the dairy farm, and decreased milk quality in dairy cattle.  Purpose: The purpose of this study was to investigate changes in milk quality in Friesian Holstein (FH) dairy cows subjected to repeat breeding at KUD Tani Wilis Sendang, Tulungagung Regency, focusing on the milk’s density, as well as fat, Total Solid (TS), and Solid Non-Fat (SNF) content. Method:  Thirty-two milk samples were collected, including 10 from normal cows for comparison. The samples were analyzed using a Lactoscan to examine the density, fat content, total solid (TS), and solid non-fat (SNF) of milk. The data was subjected to Independent T-Test analysis. Results: The results revealed significant differences (p<0.05) between repeat breeding and normal cow milk samples in terms of specific gravity, fat content, and Total Solids (TS), but not in Solid Non-Fat (SNF). Repeat breeding cow milk exhibited a higher specific gravity (1.0282 vs. 1.0260) but lower fat content (1.08% vs. 4.18%) and Total Solids (9.38% vs. 12.73%) compared to normal cow milk. However, there was no significant difference for Solid Non-Fat (8.36% for repeat breeding vs. 8.55% for normal cows). Conclusion: Repeat breeding cow milk showed increased specific gravity but decreased fat content and Total Solids compared to normal cow milk, while Solid Non-Fat remained relatively consistent.
Bruceshield: Internet of things integrated biometric and food detection system to eradicate brucellosis milk contamination Tirtono, Jasa Dwi; Prashanti, Lupita; Saputra, Raphael Abel; Suputra, I Gede Wahyudi; Permatasari, Dian Ayu
Social Agriculture, Food System, and Environmental Sustainability Vol. 2 No. 1: (February) 2025
Publisher : Institute for Advanced Science, Social, and Sustainable Future (IASSSF)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/safses.v2i1.2025.1713

Abstract

Background: Brucellosis is a zoonotic infectious disease caused by the bacteria of the genus Brucella spp. that causes abortion and chronic disease in animals and humans, resulting in economic losses worldwide. Brucellosis remains endemic in ruminant livestock in Indonesia, with a prevalence rate of 40% in ruminants and the highest incidence of cases is in Java Island. Vaccination, livestock movement monitoring, and diagnostic methods such as PCR can prevent this disease, but those methods are challenged by cost and a lack of trained personnel. Methods: The methodology used in this article is a literature review. Design ideas were identified from various international and national journal literature with the main focus on test methods consisting of Multiple Cross Displacement Amplification - Lateral Flow Biosensor (MCDA-LFB) test, Retinal Biometric system, Static QR-Code system, and Internet of Things (IoT). Findings: Bruceshield is a complete entity combining animal retinal biometrics, food detection using MCDA-LFB technology, and IoT for accurate identification, rapid detection, and effective data collection of IoT-ready devices. Some of these methods include MCDA-LFB for DNA analysis, retinal biometric systems for animal identification, and static QR codes that report parasite detection and enable traceability to the consumer in dairy products. Conclusion: Bruceshield presents an innovative solution to support the vision of 'Brucellosis Free Indonesia 2025' by integrating advanced diagnostic and monitoring systems, contributing to Sustainable Development Goal (SDG) number 3 and the One Health paradigm. This system holds the potential to enhance disease prevention, improve livestock traceability, and promote transparency and safety within the dairy and livestock industry. Novelty/Originality of this article: This study introduces Bruceshield, an integrated system combining retinal biometrics, MCDA-LFB technology, and IoT for accurate livestock identification, rapid disease detection, and effective data collection. 
IMPROVEMENT DAIRY CATTLE BARN MANAGEMENT AFTER FOOT AND MOUTH DISEASE (FMD) OUTBREAK IN MEDOWO, KEDIRI Permatasari, Dian Ayu; Raharjo, Dadik; Effendi, Mustofa Helmi; Budiarto, Budiarto
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 2 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i2.2025.260-270

Abstract

The Foot and Mouth Disease (FMD) outbreak that began in 2022 caused significant losses for dairy farmers in Medowo Village, Kandangan District, Kediri Regency—impacting both livestock health and farmers' economic stability. To address these impacts, a community service program was carried out to improve farmers' knowledge and skills in dairy cattle housing management and biosecurity implementation. The program included training sessions, educational outreach, and technical assistance for 31 farmers from KUD Kertajaya. Evaluation was conducted through pre-test and post-test instruments to measure the effectiveness of the intervention. Pre-test results showed that only 6.5% of participants were in the "good–very good" category in terms of understanding FMD and livestock management. After the intervention, post-test results showed a significant increase, with 87% of participants reaching the "good–very good" level. Additionally, in terms of practical application of cattle management practices, 100% of participants demonstrated excellent understanding after the program. This intervention proved that an educational approach combining outreach and hands-on practice can significantly enhance farmers' awareness and skills. The improvement is expected to contribute to better livestock productivity and more effective disease control. The activity also involved undergraduate students as part of the Merdeka Belajar Kampus Merdeka (MBKM) program, fostering collaboration between academia and the farming community to strengthen local capacity.
Efektivitas Model Problem Based Learning dan Make A Match dalam Pembelajaran Tematik Kelas V SD Negeri 2 Gondang Permatasari, Dian Ayu
Malih Peddas (Majalah Ilmiah Pendidikan Dasar) Vol 10, No 1 (2020): Malih Peddas, Volume 10, Nomor 1
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/malihpeddas.v10i1.5259

Abstract

AbstractAbstract: Problem-Based Learning Model and Matching in Thematic Learning at fifth grade students of 3 SD Negeri Bugangan 01 and SD Negeri 2 Gondang viewed from the appearance of critical thinking. The subjects in this study were fifth grade students at SD Negeri Bugangan 1  Elementary School as an experimental class and SD Negeri 2 Gondang Elementary School as a control class. This type of research is a quasi-experimental research type Test-Posttest Control Group Design. The Samples are 72 students. The results showed that the use of Problem Based Learning model can enhance learning about critical problems in Thematic subjects in fifth grade stdents at of 3 SD Negeri Bugangan 01 and SD Negeri 2 Gondang. It can be prove from what is supported by student’s critical thinking from the experimental class 74.21 (quite critical) and the control class 60.82 (uncritical). From the Ancova test on the calculation of Indonesian Language 1,570 level of significance or probability is 0.214. Therefore the probability is 0.214. Because of the probability 0.214 0.05, Ho is accepted, and Ha is rejected. While on the charge of Natural Sciences F count 0.334 level of significance or probability 0.565, then Ho is accepted and Ha is rejected. Related to the effective Problem Based Learning model in the Make A Match model in terms of Thematic critical thinking in fifth grade students of of 3 SD Negeri Bugangan 01 and SD Negeri 2 Gondang..Keywords: Effectiveness,Problem Based Learning and Make a Match Learning Model, identifying the type of work