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Journal : Jurnal Pembelajaran dan Biologi Nukleus

Analysis of Amylase Activity in Bacteria Isolated from Hot Spring of Pentadio Resort Nur'ain Marton Angio; Aryati Abdul; Syam S. Kumaji; Wirnangsi Din Uno; Yuliana Retnowati; Magfirahtul Jannah
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 10, No 1 (2024): Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5506

Abstract

The hot spring of Pentadio Resort is one of the geothermal areas likely to be the place of bacteria or microorganisms that can produce amylolytic enzymes. This research aims to analyze bacteria in the Pentadio Resort hot springs that possess the potential to produce amylase enzymes, analyze the characteristics of bacterial isolates that produce amylase enzymes in Pentadio Resort hot springs and determine the types of bacteria that produce amylase enzymes in Pentadio Resort hot springs. The research employs a qualitative and descriptive method. The research involved several methods, including sample collection, media preparation, isolation and selection of thermophilic bacterial isolates, purification of thermophilic bacterial isolates, selection of isolates producing amylase enzymes, identification of selected isolates (motility test, biochemical test, gram staining), and molecular identification. The research yielded seven isolates, and isolate code A6 demonstrated the potential to produce amylase enzymes with a clear zone size of 23,26 mm, bacillus, gram positive, and positive motility. Subsequently, molecular identifidation was carried out in which, based on the results of phylogenetic tree reconstractiopisalate A6 was related to the bacteria Bacillus cereus strain MD 152, which belongs to the Bacillus cereus bacteria
Co-Authors Abdul Haris PanaI Abubakar Sidik Katili Abubakar Sidik Katili Adnan Malaha Ahmad Zainuri Ahmad Zainuri Arpin Aryati Abdul Aryati Abdul Aryati Abdul Asep S. Abdurrahmat Asniwati Zainuddin Astin Lukum Astuti, Mindy Eka Aydalin, Regina Valentina Budy Santoso Chairunnisa J. Lamangantjo Chairunnisah J Lamangantjo Citron S Payu Daud Yusuf Daud, Srinianti U Daud Dewi Wahyuni K. Baderan Djuna Lamondo Eka Zahra Solikahan Eman Rahim Enisa Kobandaha Erfan AR. Lainjong Faisal Duengo Farid SM Febrianti Febrianti febriyanti febriyanti Fitryane Lihawa Harun, Nur Risnawati HASAN, ANI M. Hasan, Marwan Hasim Hasim Hasim Hasim Hasim Herinda Mardin Heru Z Thalib Husain, Ilyas Isra, Muhammad Iswan Dunggio Jusna Ahmad La Nane Lamangantjo, Chairunnisa J. Lilan Dama Mabuia, Siti Ainun Magfirahtul Jannah Margaretha Solang Marike Mahmud Marini Susanti Hamidun Masra Latjompoh Misnawaty Wantogia Moh. Heru Sulistyo P. Yusuf Moha, Siti Nurliana Mohamad Haikal K. Djafar Mohamad, Nurdin Muh. Nur Akbar Muhammad Isra Mustaqimah, Nur Nur Risnawati Harun Nur'ain Marton Angio Nurfadila Salam Nurinda Umadji Pinangsi Lalu Pobela, Juliani Pratama, M.Iqbal Liayong Rakhmat Jaya Lahay Ramli Utina Regina Valentine Aydalina Regitacahyanisauring Gita Rini Daud Supu Sadapu, Rifka Salihi, Irvan Abraham Sarini Pani SM, Farid Solang, Margareta Sri Anggriyanti Putri Bohoka Sri Vanrovia Usman Sri Widyastuty Pikoli Sri Wulandari Sri Wulandari Sukirman Rahim Suma, Fani Syarifudin Rahim Thalib, Heru Z Torana, Melanie Uno, Wirnangsi D. Wantogia, Misnawaty Widya Rahmawaty Saman Widya Salandri Kisman Wirnangsi D. Uno Wirnangsi D.Uno Wirnangsih D. Uno Wiwin Kobi Yuliana Retnowati Yulianti Humokor Yulinda L. Ismail Zakaria Husain Zuliyanto Zakaria