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Journal : JHTS

PENGARUH EKSTRAK DAUN KETEPENG CINA (Cassia alata L.) TERHADAP PERTUMBUHAN JAMUR Malassezia furfur PENYEBAB KETOMBE Moh. Heru Sulistyo P. Yusuf; Sarini Pani; Syam S. Kumaji
Journal of Health, Technology and Science (JHTS) Vol. 1 No. 1 (2020): Journal of Health, Technology and Science (JHTS)
Publisher : LPPM Universitas Bina Mandiri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.537 KB) | DOI: 10.47918/jhts.v1i1.19

Abstract

Daun Ketepeng Cina (Cassia alata L.) sering digunakan sebagai obat tradisional dalam menyembuhkan jamur kulit penyebab bercak putih. Namun sebagian besar masyarakat di perkotaan Manado meng-gunakan daun ketepeng Cina sebagai sampo. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun Ketepeng Cina terhadap pertumbuhan jamur Malassezia furfur penyebab ketombe dan untuk mengetahui konsentrasi optimum daun Ketepeng Cina terhadap per-tumbuhan jamur Malassezia furfur penyebab ketombe. Metode eksperimental laboratorium yang digunakan dalam pene-litian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 6 kali perlakuan dengan 4 kali ulangan. Konsentrasi daun Ketepeng Cina yang digunakan pada penelitian kali ini adalah 0,5%, 1%, 1,5%, dan 2% dengan kontrol positif Ketokonazol 2%. Objek penelitian ini adalah pertumbuhan jamur Malassezia furfur. Hasilnya, setiap konsentrasi ekstrak daun ketepeng cina dan kontrol negatif (metanol) menunjukkan bahwa tidak ada zona hambat yang terbentuk terhadap pertumbuhan jamur Malassezia furfur penyebab ketombe, apalagi kontrol positif (Ketoconazole 2%) menunjukkan ada-nya zona hambat sekitar 17,98 mm. Berdasarkan hasil ini dapat disim-pulkan bahwa ekstrak daun Ketepeng Cina (Cassia alata L.) berpenga-ruh pada pertumbuhan jamur Malassezia furfur penyebab ketombe
IDENTIFICATION OF Escherichia coli BACTERIA IN QUAIL EGGS THAT FOR SALE IN THE CENTRAL MARKET OF THE CITY OF GORONTALO Regitacahyanisauring Gita; Syam S. Kumaji; Erfan AR. Lainjong
Journal of Health, Technology and Science (JHTS) Vol. 2 No. 2 (2021): Journal of Health, Technology and Science (JHTS)
Publisher : LPPM Universitas Bina Mandiri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.296 KB) | DOI: 10.47918/jhts.v2i1.100

Abstract

Eggs are one of the animal products that come from poultry and have been known as a source of high quality protein food. From the many eggs we know, one of the eggs that comes from poultry is quail eggs. Quail eggs have complete nutritional content ranging from protein, fat, vitamins and minerals. Even so, quail eggs are also animal food that is easily decreased in quality due to bacterial contamination, contamination of quail eggs by bacteria can affect the quality of the contents of the eggs, bacteria that cause egg contamination, one of which is Escherichia coli. This study aims to determine the presence of Escherichia coli bacteria in quail eggs that are sold in the central market of Gorontalo city. The research method used is descriptive research type with a quantitative research approach, cross sectional research design, a sampling place for the central market of Gorontalo City and a place for researching samples of the Microbiology Laboratory of Gorontalo State University, the number of samples used was 30 samples of quail eggs. Quail eggs were positive for Escherichia coli bacteria with a percentage of 6.7%, and negative egg yields from Escherichia coli bacteria were 28 or 93.3%. It can be concluded that from 30 samples of quail eggs there were 2 quail eggs that were positive for Escherichia coli bacteria, the bacteria contamination factor in eggs was influenced by external factors. Environmental sanitation conditions were not clean, egg storage temperature, egg storage time,
POTENTIAL OF ENDOPHY BACTERIAL ISOLATE FROM LEAF OF CHINESE TEMPERATURE (Cassia alata L) ON THE GROWTH OF THE BACTERIA OF STAPHYLOCOCCUS AUREUS Enisa Kobandaha; Syam s kumaji; Adnan Malaha
Journal of Health, Technology and Science (JHTS) Vol. 3 No. 1 (2022): Journal of Health, Technology and Science (JHTS)
Publisher : LPPM Universitas Bina Mandiri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.49 KB) | DOI: 10.47918/jhts.v3i1.332

Abstract

ABSTRACT This research aims to To determine the effect of endophytic bacterial isolates from the leaves of the Chinese Ketepeng plant (Cassia Alata L) on the growth of Staphylococcus aureus and to determine the significant difference between each treatment of endophytic bacterial isolates from the leaves of the Ketepeng Cina (Cassia Alata L) plant against Staphylococcus aureus. This research method used a completely randomized design (CRD) with 4 treatments and 6 replications. The treatments were: BEKC 1 (Chinese Ketepeng Endophytic Bacteria 1), BEKC 2 (Chinese Ketepeng Endophytic Bacteria 2), Positive Control (Chloramphenicol), and Negative Control (Aquades). The results obtained two isolates of endophytic bacteria, namely isolates BEKC 1 with an inhibition zone of 9mm included in the medium category, and isolates BEKC 2 with an inhibition zone of 10mm included in the strong category. Analysis resultthat there was an effect of endophytic bacterial isolates from the leaves of the Chinese ketepeng plant (Cassia alata L) on the growth of Staphylococcus aureus bacteria. Then from the results of further tests using Duncan's test, it was found that each treatment, namely the negative control treatment was significantly different from BEKC 1, BEKC 2 and positive control, while there was no significant difference between BEKC 1, BEKC 2 and positive control. Keywords : Endophytic Bacteria, Chinese Ketepeng (Cassia alata L), Staphylococcus aureus
Bahasa Inggris Syarifudin Rahim; Mindy Eka Astuti; Syam S Kumaji
Journal of Health, Technology and Science (JHTS) Vol. 1 No. 2 (2020): Journal of Health, Technology and Science (JHTS)
Publisher : LPPM Universitas Bina Mandiri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47918/jhts.v1i2.404

Abstract

The purpose of this study was to determine the results of the analysis of Coliform bacterial contamination in tomato sauce snacks on Jalan Toto Tengah, Gorontalo City.This research method is descriptive observational with a qualitative approach. The method in this study uses the MPN method which consists of the estimator test, reinforcement test and Gram staining and uses the MPN 333 table according to Thomas's formula.The results showed that all samples of tomato sauce tested positive for Coliform bacteria and did not meet the standards based on BPOM Standard number HK.00.06.1.52.4011 in 2009 concerning the maximum limit of microbial and chemical contamination in food with a maximum APM Coliform of 100 APM/g. estimator test all samples change color to yellow. Meanwhile, in the reinforcement test results, 1 sample had metallic green bacterial colonies and 5 other samples had pink colonies and for Gram staining, rod-shaped Gram negative bacteria were obtained.
Co-Authors Abdul Haris PanaI Abubakar Sidik Katili Abubakar Sidik Katili Adnan Malaha Ahmad Zainuri Ahmad Zainuri Arpin Aryati Abdul Aryati Abdul Aryati Abdul Asep S. Abdurrahmat Asniwati Zainuddin Astin Lukum Astuti, Mindy Eka Aydalin, Regina Valentina Budy Santoso Chairunnisa J. Lamangantjo Chairunnisah J Lamangantjo Citron S Payu Daud Yusuf Daud, Srinianti U Daud Dewi Wahyuni K. Baderan Djuna Lamondo Eka Zahra Solikahan Eman Rahim Enisa Kobandaha Erfan AR. Lainjong Faisal Duengo Farid SM Febrianti Febrianti febriyanti febriyanti Fitryane Lihawa Harun, Nur Risnawati HASAN, ANI M. Hasan, Marwan Hasim Hasim Hasim Hasim Hasim Herinda Mardin Heru Z Thalib Husain, Ilyas Isra, Muhammad Iswan Dunggio Jusna Ahmad La Nane Lamangantjo, Chairunnisa J. Lilan Dama Mabuia, Siti Ainun Magfirahtul Jannah Margaretha Solang Marike Mahmud Marini Susanti Hamidun Masra Latjompoh Misnawaty Wantogia Moh. Heru Sulistyo P. Yusuf Moha, Siti Nurliana Mohamad Haikal K. Djafar Mohamad, Nurdin Muh. Nur Akbar Muhammad Isra Mustaqimah, Nur Nur Risnawati Harun Nur'ain Marton Angio Nurfadila Salam Nurinda Umadji Pinangsi Lalu Pobela, Juliani Pratama, M.Iqbal Liayong Rakhmat Jaya Lahay Ramli Utina Regina Valentine Aydalina Regitacahyanisauring Gita Rini Daud Supu Sadapu, Rifka Salihi, Irvan Abraham Sarini Pani SM, Farid Solang, Margareta Sri Anggriyanti Putri Bohoka Sri Vanrovia Usman Sri Widyastuty Pikoli Sri Wulandari Sri Wulandari Sukirman Rahim Suma, Fani Syarifudin Rahim Thalib, Heru Z Torana, Melanie Uno, Wirnangsi D. Wantogia, Misnawaty Widya Rahmawaty Saman Widya Salandri Kisman Wirnangsi D. Uno Wirnangsi D.Uno Wirnangsih D. Uno Wiwin Kobi Yuliana Retnowati Yulianti Humokor Yulinda L. Ismail Zakaria Husain Zuliyanto Zakaria