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All Journal Jurnal Kesehatan Prima Journal of Maternal and Child Health Jurnal Analis Medika Biosains (JAMBS) Jurnal Keperawatan Terpadu (Integrated Nursing Journal) Poltekita : Jurnal Ilmu Kesehatan Hydrogen: Jurnal Kependidikan Kimia Jurnal Pengabdian Masyarakat Sasambo Jurnal Keperawatan Indonesia Timur (East Indonesian Nursing Journal) Jurnal Borneo Cendekia The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Media Penelitian dan Pengembangan Kesehatan Journal of Indonesia Laboratory Students (JILTS) Thrive Health Science Journal

Yunan Jiwintarum

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Author-ID : 292555

Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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GOLD IMMUNOCHROMATOGRAPHIC ASSAY (GICA) SEBAGAI IMUNOSENSOR MENDETEKSI ANTIBODI Bacillus Anthracis PENYEBAB PENYAKIT ZOONOSIS Gunarti Gunarti; Yunan Jiwintarum; Awan Dramawan; Lale Budi Kusuma Dewi
Jurnal Kesehatan Prima Vol 9, No 1 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: The purpose of this study was to identify patterns of protein antigens full / secretory antigens may be antigenic protein (PA) of Bacillus anthracis by the use of SDS-PAGE method (Laemmli) which can be used as bioreseptor on the test device fabrication method for detecting antibodies GICA of Bacillus anthracis , identify patterns of proteins that are antigenic protein (PA) or indicate the nature of the reactivity of Bacillus anthracis by using Western blotting and Determining the specificity and sensitivity of the test method to detect antibodies GICA Bacillus anthracis when using the results of the ELISA method as a reference. This study is a descriptive observational study. Research variable antigenic protein of Bacillus anthracis as bioreseptor and Antibodies from Bacillus anthracis as imunosenssor. The test prototype test device made using 20 sample with ELISA as a reference for calculating the sensitivity and specificity. The results of this study were identified band – band of 92 kDa, 68 kDa, 54 kDa, 43 kDa, 29 kDa and 18 kDa that can be used as bioreseptor on GICA, identified a pattern of proteins that are antigenic or indicate the nature of the reactivity of the Bacillus anthracis 68 kDa by Western blotting using the method, extract secretory antigen (SA) Bacillus anthracis can be used as a test device bioreseptor on GICA method for the detection of IgG antibodies against Bacillus anthracis and sensitivity and specificity of the test method to detect antibodies GICA Bacillus anthracis when using the results of the ELISA method as a reference each is 100% and 87.5%.

PENGARUH PERBEDAAN SUHU TERHADAP POTENSI TABLET SIPROFLOKSASIN (EKSPERIMEN DENGAN BAKTERI Escherichia coli) Rauhul Akbar Kurniawan; Yunan Jiwintarum; Liana Dwihartati
Jurnal Kesehatan Prima Vol 9, No 2 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: It has been conducted the determination of potency of Ciprofloxacin 500 tablets was storage in temperature 0°C, 10°C, 20°C, 30°C, 40°C for 20 hour. The aim of research is to find out the effect of different temperature for potency of Ciprofloxacin tablets. The method of determination applied is the measure diameter zone inhibition Escherichia coli in MHA. The result indicated that was negative interaction temperature storage for potency of Ciprofloxacin tablets.

DRAGON FRUIT (Hylocereus polyrhizus) AS NATURAL DYES STAINING FOR BACTERIA Yunan Jiwintarum; Rohmi Rohmi; I Dewa Putu Martha Prayuda
Jurnal Kesehatan Prima Vol 10, No 2 (2016): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract : the bacteria is difficult to be perceived with a light microscope because it can not adsorb or refract the light, consequently the dye applies for staining the bacteria or their background. Synthetic dye has a fairly expensive price than natural dye. This study aims to determine the usage of dragon fruit (Hylocereus polyrhizus) as a natural dye for staining the bacteria. This research is quasi experimental, while the data will be analysed by descriptive analysis with 3 treatments, in each uses eight samples. The study showed that on the quality of stains, bacterium Staphylococcus aureus and E. coli by using the juice of the dragon is obtained a result, as many as 4 slides preparations of bacteria Staphylococcus aureus (33.33%) and 4 slides preparations of bacteria E .coli (33.33%) were not stained. Therefore, the result on the research can be concluded that the dragon fruit (Hylocereus polyrhizus) can not be used as a natural dye for staining bacteria.

NATURAL MEDIA FOR THE GROWTH OF CANDIDA ALBICANS CAUSES OF CANDIDIASIS BY ARTOCARPUS COMMUNIS Yunan Jiwintarum; Urip Urip; Anas Fadli Wijaya; Maruni Wiwin Diarti
Jurnal Kesehatan Prima Vol 11, No 2 (2017): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

a candida albicans requires a source of nutrients especially carbohydrates that are sufficient for its growth. Semi-synthesis media is the most common medium for growing Candida albicans. Semi-synthesized media composes of natural and synthesis materials. An abundance of biological sources in nature with higher carbohydrate content than natural ingredients on semi-synthesized media encourage to find the materials of alternative media from different carbohydrate sources, particularly grains, one of them is breadnut seed. This study aimed to find out a potential of Artocarpus communis as an alternative media for the growth of Candida albicans causes of candidiasis. This study was true experiment, used 6 replications and 4 treatments with PDA as control media, the media of Artocarpus communis with concentration of 10%, 20%, and 30%. The data of Candida albicans growth was tested statistically used One Way Anova with 95% confidence level or α = 0.05. The result indicated that a number of Candida albicans growth on PDA media was 13.7 colonies, on alternative media of Artocarpus communis with the concentration of 10% was an average of 13.5 colonies, the concentration of 20% was an average of 16.3 colonies and the concentration of 30% was an average of 24 colonies. The conclusion: the Artocarpus communis can be employed as an alternative natural media of Candida albicans growth.

TOTAL KOLESTEROL CONDITIONS ON CONSUMPTION OF BLACK POWDER COFFEE (Traditional) WITH PATIENT PREPARATION Maruni Wiwin Diarti; Yunan Jiwintarum; Ida Ayu Reka
Jurnal Kesehatan Prima Vol 12, No 1 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: A traditional black coffee powder is one of the beverages that excessively consumed by communities. The increasing of total cholesterol level in blood is influenced by the habit of consuming coffee. Coffee has chemicals that can cause some health problems, namely cafestol and kahweol. The objective study was to determine the effect of patient preparation against the result of total cholesterol levels examination on the consumers of traditional black coffee powder. This research was analytic observational. The sample was capillary blood of consumers’ a traditional black coffee as many as 30 people. Statistical analysis utilized Mann-Whitney U Test with 95% confidence level (α = 0,05). The findings indicated the average deviation of total cholesterol level after fasting 12,86 mg / dL and mean difference after giving coffee 7 cups 18,46 mg / dL. Mann Whitney Test found significant value 0.001 <α = 0.05 which means there is an influence of patient preparation against the result of total cholesterol levels examination on the consumers of traditional black coffee powder.Keywords: Total Cholesterol Level; Consumers of Traditional Black Coffee Powder.

A CHARACTERISTIC OF MORPHOLOGY, COLONY AND BIOCHEMISTRY OF BACTERIA THAT ISOLATED FROM SEDIMENTS OF MOSQUITO BREEDING LAGOON Maruni wiwin Diarti; Rohmi rohmi; Yuri Syaffitri Kinanti Achmad; Yunan Jiwintarum
Jurnal Kesehatan Prima Vol 11, No 2 (2017): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

The lagoon is a form of semi-enclosed bay which is one type of an estuary. In lagoon water there is a meeting and mixing of freshwater and seawater thus it causes free relationship between the sea and the source of freshwater. The effect of tides that mixed with freshwater will cause a change of the aquatic character instantly. It will certainly affect the diversity of organism species, including bacteria. Some types of bacteria, one of them is Bacillus, it is basically soil bacteria, but commonly found in fresh and brackish waters. The purpose of this study was to determine the characteristics of morphology, colonies and biochemistry of bacteria that isolated from sediments of mosquito breeding lagoon in the coast of Medana village, Tanjung district, North Lombok regency. This research was descriptive observational, used non random purposive sampling with 15 samples. Data collected in the form of types of bacteria found in 15 points from sediments of mosquito breeding lagoon. The data analysis used descriptive analysis. The results of 15 points of lagoon sediments that isolated, from 15 bacteria colonies that was identified as morphological, colonies and biochemical characteristics found three types of bacteria: Aeramonas schubertii, Bacillus subtilis, and Enterbacter agglomerans. The conclusion is the sediments of mosquito breeding lagoon in the coast of Medana Village, Tanjung district, North Lombok regency is obtained the bacteria, for instace: Aeramonas schubertii, Bacillus subtilis, and Enterobacter agglomerans.

THE EFFECT OF MELON SEEDS FLOUR (Cucumis Melo L.) TOWARD TOTAL CHOLESTEROL LEVEL IN WHITE MALE WISTAR STRAIN RATS (Rattus Norvegicus) Maruni Wiwin Diarti; Erlin Yustin Tatontos; Yunan Jiwintarum; Anggun Sari Mianti
Jurnal Kesehatan Prima Vol 12, No 2 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Cholesterol is an important element in the body, but in high quantities, it can cause atherosclerosis that will eventually possess an impact on coronary heart disease. One of the foods that can reduce cholesterol is a food contains flavonoids which is found in melon seeds (Cucumis melo l.). It contains high fiber that can reduce either of excessive insulin or blood fat levels. The objective study was to determine the effect of Melon seeds flour Toward Total Cholesterol Level in White Male Wistar Strain Rats. This research was carried out with pre-expriment research design, used One group pretest-postest with 5 male wistar strain rats. Data collected in the form of data examination results of total cholesterol level before and after introducing Melon seeds flour. The mean result of total cholesterol test in white male rats before giving Melon seeds flour was 261 mg / dL, whereas the result of total cholesterol test after giving Melon seeds flour was 190.2 mg/dL. Cholesterol levels decreased by 70.8 mg/dl. In short, presenting Melon seeds flour (Cucumis melo l.) for 14 days can decrease total cholesterol level in white male wistar rats. Keywords: Melon Seeds, wistar strain, total cholesterol.

RESISTENSI PRIMER MYCOBACTERIUM TUBERCULOSIS DENGAN TARGET Gen rpoB Yunan Jiwintarum; Maruni Wiwin Diarti; Awan Dramawan
Jurnal Kesehatan Prima Vol 8, No 1 (2014): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Detection of primary resistance in sputum samples of patients with pulmonary tuberculosis AFB (+) with molecular analysis on the target rpoB gene causes anti-tuberculosis drug resistance (OAT) rifampicin can be beneficial to the holder of a policy program to look for tuberculosis control efforts, one of attempt to overcome these problems is to deliver information about the resistance report in this interval. This research observasional descriptive study, the research variables Mycobacterium tuberculosis rpoB gene mutations and patients with pulmonary TB sputum smear (+), with the number of 50 sputum samples with acidental sampling technique. This study results showed all the samples (50 samples) microscopic examination of smear and PCR diagnostics with primary genes and gene Tb1-Tb2 results are positive (+) tuberculosis (100%). By nested PCR amplification techniques to prove that the primary gene rpoB1 negative results of 23 samples (46%), with the primary gene rpoB2 negative results in 13 samples (26%). Conclusion: A total of 13 samples (26%) occurred mutation in the gene rpoB / is resistant to the antibiotic rifampin.

SENSITIVITAS MEDIA OGAWA DAN MEDIA LOWENSTEIN JENSEN TERHADAP HASIL PERTUMBUHAN KUMAN MYCOBACTERIUM TUBERCULOSIS Pancawati Ariami; Maruni Wiwin Diarti; Yunan Jiwintarum
Jurnal Kesehatan Prima Vol 8, No 2 (2014): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Referring to the national TB control program, the diagnosis made by microscopic examination of sputum by direct and definitive diagnosis by culture examination. Direct sputum examination is the gold standard method of microscopic examination of a recognized WHO TB and confirmation done by the method of culture. Research "Sensitivity Test Using Ogawa Medium and Lowenstein Jensen Medium Againts Micobacterium tuberculosis Bacteria Growth Results ", performed to identify and compare the results of Ogawa medium and culture on Lowenstein-Jensen medium and analyze the sensitivity between the two medium. A descriptive observational study was conducted. Study sample is a sample saturated with a consecutive sampling. Sample size retrieval technique based on sample collection time, which is June 8 through July 18, 2011, a total of 53 samples. Research is to test the sensitivity variables as independent variables and the growth of Mycobacterium tuberculosis bacteria on the Ogawa medium and LJ medium as a bound variable. The results of the study, of 53 samples, 21 (39.6%) samples were negative, so that 32 (60.4%) samples tested positive for M tuberculosis and the total growth of 36 samples (67.9%). Lowenstein Jensen (LJ) medium is more sensitive than the Ogawa medium, both in terms of speed of growth and in the number of colonies that grow. For the determination of Mycobacterium species needs to be done other tests to confirm. Culture results can also be used for comparison using the PCR method, OAT resistance by culture methods.

PERBEDAAN HASIL UJI KOAGULASE MENGGUNAKAN PLASMA SITRAT MANUSIA 3,8%, PLASMA SITRAT DOMBA 3,8%, DAN PLASMA SITRAT KELINCI 3,8% PADA BAKTERI Staphylococcus aureus Yunan Jiwintarum; Lalu Srigede; Auliya Rahmawati
Jurnal Kesehatan Prima Vol 9, No 2 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Coagulase test is a bacterial examination performed to detect the formation of coagulase enzyme bound to the bacterial cell wall. This study aims to determine the difference in the form of a coagulase test results clot formation by using several types of plasma citrate in bacteria Staphylococcus aureus. This type of research is true experimental replication using 9 and 3 human citrate plasma treatment is 3.8%, 3.8% sheep plasma citrate, and 3.8% rabbit citrate plasma. The results show the average time of the formation of clots in the coagulase test using 3.8% human citrate plasma was 1:29 minutes, using sheep plasma citrate 3.8% was 00:54 minutes, and using 3.8% rabbit citrate plasma is 1:40 minutes. Results of One Way Anova statistical test obtained p value count (0,00) <p α (0.05), meaning that there are significant differences in the results of coagulase test using human plasma citrate 3.8%, 3.8% sheep plasma citrate, and rabbit plasma citrate 3.8% in Staphylococcus aureus.

Co-Authors AA Sudharmawan, AA Achmad Fathoni Adawiyah, Luluil Aji Kresnapati, I Nyoman Bagus Anas Fadli Wijaya Anggun Sari Mianti Aprilla, Annisa Suci Ari Khusuma Asyhaer, Rifki Khalidi Atmojo, Joko Tri Auliya Rahmawati Awan Dramawan Ayu Anulus Ayu Rosita Ã¢â‚¬â„¢diah, Halimatussa Dektra, I Made Putra Pramaditya Dewi, Lale Budi Kusuma Duarsa , Artha Budi Susila Eliza, Ida Eliza, Ida Erdianti Nursahar Erlin Yustin Tatontos Ershandi Resnhaleksmana Fachrudi Hanafi Fihiruddin, Fihiruddin Fitria Ernawati Getas, I Wayan Gunarti Gunarti Gunarti, Gunarti Habib Sasiwimbe Hapielda, Ririn Hardiyanti, Uci Haris Widita Herawati, Lale Heny I Dewa Putu Martha Prayuda I Gede Angga Adnyana I Putu Bayu Agus Saputra Ida Ayu Reka Iswari Pauzi Iswidhani, Iswidhani Khairunnisa Kresnapati, I Nyoman Bagus Aji Lalu Srigede Lalu Srigede, Lalu Liana Dwihartati Mardiatuz Zahra Marnia, Marnia Maruni Wiwin Diarti Maruni Wiwin Diarti Nurhidayati Nurhidayati Pancawati Ariami Pauzi, Iswari Putra, I Gede Ari Permana Rahmani, Putri Ziddanur RAI WIADNYA, IDA BAGUS Rauhul Akbar Kurniawan Resnhaleksmana, Dr.Ersandhi Resnhaleksmana, Ersandhi Retno Handayani Retno Handayani Rohmi Rohmi Rohmi, Rohmi saadaty, nila uthari Sahputri, Maulidia Rohmiati Salsabila, Nadira Shohifatul Wahyuni Sintia Dewi, Ni Kadek Siti Zaetun, Siti Soewignjo Soemohardjo Suda Adnyana Yoga, I Gede Sukma, Al Hadawiyah Pertiwi Sukmawati, Sulvani Sulaimah, Rabi'unnisa Sulvani Sukmawati Troef Sumarno Urip Urip Urip Urip Urip, Urip Wahyuni, Shohifatul Wayan Getas, Drs.I Weny Astuti Widianto, Aris Yuliana Wardani Yuri Syaffitri Kinanti Achmad Zaeniati, Baiq Lely Zainal Fikri Zainal Fikri Zainal Fikri, Zainal Zainul Mutaqin ’diah, Halimatussa

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