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All Journal Agrotrop : Journal on Agriculture Science SOCA: Jurnal Sosial Ekonomi Pertanian Jurnal Kimia (Journal of Chemistry) International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Jurnal SPEKTRUM Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Ekonomi dan Bisnis Jagaditha Agrovigor: Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA AGROMIX Jurnal Entomologi Indonesia Jurnal Widya Biologi Agrica: Journal of Sustainable Dryland Agriculture SEAS (Sustainable Environment Agricultural Science) Journal of Sustainable Development Science
I Putu Sudiarta
Magister Of Agricultural Biotechnology, Faculty Of Agriculture Of Udayana University, Jl. PB. Sudirman, Denpasar 80231 Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health

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The Effect of media types on the growth of insect pathogenic fungi (Aschersonia placenta) Sudiarta, I Putu; Hartha , I Komang Gede Suweca; Yuliadhi , Ketut Ayu; Wirya, Gusti Ngurah Alit Susanta; Selangga, Dewa Gede Wiryangga; Suputra , I Putu Wirya; Listihani, Listihani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12554-62

Abstract

Bali has favorable conditions for the production and expansion of citrus plantations. However, citrus cultivation in Bali often faces challenges, including reduced yield caused by pest infestations and pathogenic diseases. One significant pest affecting citrus plants is the whitefly. A potential method for controlling whiteflies is the use of natural enemies. The entomopathogenic fungus Aschersonia placenta is one of natural enemy that can effectively manage whitefly infestations. However, the succesful utilization of A. placenta requires a specialized approach, particularly in selecting an appropriate growth medium. This study aimed to investigate the impact of different growth media on the development of the entomopathogenic fungus A. placenta. The research began with field sampling, followed by the isolation and morphological identification of A. placenta to obtain a pure culture. Subsequently, media tests were conducted to evaluate fungal growth. Nine treatments were implemented to examine the impact of different media: (1) Water Agar (WA), (2) Potato Dextrose Agar (PDA), (3) Potato Sucrose Agar (PSA), (4) Water Agar + Weaver Ant Eggs Flour (WA-WAEF), (5) Potato Dextrose Agar + Weaver Ant Eggs Flour (PDA -WAEF), (6) Potato Sucrose Agar + Weaver Ant Eggs Flour (PSA-WAEF), (7) Water Agar + Cricket Flour (WA-CF), (8) Potato Dextrose Agar + Cricket Flour (PDA-CF), and (9) Potato Sucrose Agar + Cricket Flour (PSA-CF). The results demonstrated that incorporating cricket insect flour (Gryllidae: Orthoptera) or weaver ant egg flour (Oecophylla smaragdina) into potato sucrose media provided the most optimal growth environment for A. placenta.
Detection and molecular identification of citrus stem rot disease in Bali Province Wirya, Gusti Ngurah Alit Susanta; Agung, I Dewa Agung Putra; Sudiarta, I Putu

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v16i1.4075

Abstract

One major disease of citrus is basal stem rot which may cause significant constraint on citrus production in Indonesia. Basically the diseasecan be caused by fungi Phytophthora spp., Diplodia sp., and Botryodiplodia theobromae or Lasiodiplodia theobromae. The purpose of this study wasto provide information on the main pathogens and characterization of BPB disease, especially in citrus in Bali, which ultimately leads to early warning for protection against BPB disease and enriches academic evidence about pre-existing BPB disease.The method used is observation andisolation, pathogenicity test, and in vitro inhibitory test. Research activities include (1) sampling, (2) isolation of pathogens from symptomatic citrusplants, (3) test of pathogenicity, (4) identification of pathogen morphology and molecular, (5) DNA amplification, (6) DNA electrophoresis (7) analysis of DNA sequences. The results of this research successfully identified Lasiodiplodia theobromae, as a pathogen of citrus stem rot disease in Bali Province.
IDENTIFIKASI JAMUR PENYEBAB PENYAKIT BUSUK BATANG ANGGREK DAN UJI PATOGENISITASNYA PADA BEBERAPA GENUS ANGGREK Putra, I Gusti Putu Semara; Wirya, Gusti Ngurah Alit Susanta; Sudiarta, I Putu
AGRICA Vol. 18 No. 1 (2025): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v18i1.5041

Abstract

Plant pests and diseases are barriers to orchid cultivation. These pests and diseases can harm and even destroy cultivated orchids, resulting in losses for producers. This study aims to identify and test fungal infections that cause stem rot in Dendrobium sp., Vanda sp., and Phalaenopsis sp. orchids. The study was carried out at Udayana University's Plant Disease Laboratory, Plant Protection Concentration, Agroecotechnology Study Program, and Faculty of Agriculture. The Fusarium fungus was discovered to be the primary cause of stem rot in Dendrobium sp. marcophyllum orchids after sampling them. The application of Fusarium fungus to three orchid genera, Dendrobium sp., Vanda sp., and Phalaenopsis sp., revealed that Phalaenopsis sp. orchids experienced a significant increase in infection percentage, beginning at 20% in the first week and peaking at 70% in the fifth week, indicating a high susceptibility to the disease. Meanwhile, Dendrobium sp. showed a steadier infection rate, beginning at 10% in the first week and progressively growing to 40% by the fifth week. Vanda sp. exhibited the lowest infection rate, rising from 10% in the first week to 20% by the fifth week, demonstrating a significantly greater resistance than the other two species.
Detection and molecular identification of citrus stem rot disease in Bali Province Wirya, Gusti Ngurah Alit Susanta; Agung, I Dewa Agung Putra; Sudiarta, I Putu
AGROMIX Vol 16 No 1 (2025)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v16i1.4075

Abstract

One major disease of citrus is basal stem rot which may cause significant constraint on citrus production in Indonesia. Basically the diseasecan be caused by fungi Phytophthora spp., Diplodia sp., and Botryodiplodia theobromae or Lasiodiplodia theobromae. The purpose of this study wasto provide information on the main pathogens and characterization of BPB disease, especially in citrus in Bali, which ultimately leads to early warning for protection against BPB disease and enriches academic evidence about pre-existing BPB disease.The method used is observation andisolation, pathogenicity test, and in vitro inhibitory test. Research activities include (1) sampling, (2) isolation of pathogens from symptomatic citrusplants, (3) test of pathogenicity, (4) identification of pathogen morphology and molecular, (5) DNA amplification, (6) DNA electrophoresis (7) analysis of DNA sequences. The results of this research successfully identified Lasiodiplodia theobromae, as a pathogen of citrus stem rot disease in Bali Province.
Eksplorasi Jamur Entomopatogen, Dari Beberapa Rizosfer Tanah, Dengan Insect Bait Method Sudiarta, I Putu; Syahbana , Raditya Darmawan; Yuliadhi, Ketut Ayu; Suputra, I Putu Wirya; Gargita, I Wayan Diksa
AGRICA Vol. 17 No. 1 (2024): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v17i1.4146

Abstract

This study aims to identify the types of entomopathogenic fungi obtained from the exploration results of the insect bait method in rhizosphere soil samples from several locations in the Bedugul area, Bali. The exploration results showed differences among the three soil samples, where the Pakcoy cultivation soil sample showed infected insect baits with Metarhizium sp. fungi. In contrast, in the Danau Buyan and Bedugul Botanical Garden soil samples, symptoms of infestation were not infected by fungi. Koch's postulate test confirmed the presence of entomopathogenic fungi in the Pakcoy cultivation soil sample, which was later identified morphologically and microscopically as Metarhizium. This study contributes to understanding the ecology of entomopathogenic fungi in the context of biological pest control, especially in sustainable agriculture.
Effect of Papaya ringspot virus watermelon strain on growth, yield and quality of melon Selangga, Dewa Gede Wiryangga; Temaja, I Gede Rai Maya; Wirya, Gusti Ngurah Alit Susanta; Sudiarta, I Putu; Listihani, Listihani; Hanifah, Wafa’ Nur; Maulinda, Restiana
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 1 (2026): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.126180-189

Abstract

Papaya ringspot virus watermelon strain (PRSV-W) is a member of the genus Potyvirus that infects Cucurbitaceae crops. The first occurrence of PRSV-W in Indonesia was reported in Bali in 2022. However, information regarding yield loss caused by PRSV-W infection in melon has not yet been reported. This study aimed to analyze yield loss and changes in fruit quality of melon resulting from PRSV-W infection. The research methods included individual and population level disease observations, assessment of agronomic variables and disease severity, yield loss estimation, and data analysis. Observations were conducted at the Pegok Experimental Farm, Faculty of Agriculture, Udayana University, covering an area of 1000 m². The observed plant ages ranged from 6 to 66 days after planting (dap). Mosaic disease development was observed from 18 dap until before harvest. The highest disease incidence and disease severity recorded at the end of the observation period were 58.4% and 61.8%, respectively. Actual yield loss, calculated by comparing mosaic symptomatic and asymptomatic melon plants, reached 49.45%. The most prominent quality defects were changes in fruit color and shape and fruit malformation, with values of 14.06% and 17.5%, respectively. The greatest economic loss occurred in plots with an AUDPC value of 751, resulting in a total yield loss of IDR 15,721,500. Yield loss showed strong correlations with disease incidence and disease severity, with correlation values of 95.41% and 96.19%, respectively. PRSV-W infection altered fruit skin color from yellow-orange to pale yellow with ringspot symptoms, and PRSV-W was detected in the skin tissues of infected fruits. In addition, PRSV-W infection reduced fruit sweetness to 9–12 °Brix, whereas healthy fruits exhibited sweetness levels of 14–17 °Brix.
EKSPLORASI JAMUR ENTOMOPATOGEN DARI TANAH DATARAN RENDAH DENGAN INSECT BAIT METHOD UNTUK MENGENDALIKAN HAMA Spodoptera frugiperda JE. Smith (Lepidoptera:Noctuidae) Anu Watu, Getrud Novia; Sudiarta, I Putu; Darmiati, Ni Nengah; Yuliadhi, Ketut Ayu; Singarsa, I Dewa Putu; Gargita, I Wayan Diksa
AGRICA Vol. 18 No. 2 (2025): December
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v18i2.6233

Abstract

Corn (Zea mays L.) is one of the leading food commodities in Indonesia whose availability is often not optimally met. The decline in productivity in agricultural land caused by pest attacks is a very damaging and detrimental factor. Biological control using biological agents in the form of entomopathogenic fungi is an alternative choice for controlling corn pests that is quite cheap but environmentally friendly so as to maintain the balance of the environmental agro-ecosystem. The purpose of this research is to identify the types of entomopathogenic fungi from exploration using the insect bait method in several lowland soil samples that have great potential as biological agents to control Spodoptera frugiperda pests that attack corn plants. The results of the study showed that the lowland soil samples of Tojan, Klungkung and Saba, Blahbatuh, Gianyar did not find symptoms of entomopathogenic fungi that infect the insect pest S. Frugiperda, while the lowland soil samples of Padanggalak, Sanur found symptoms of entomopathogenic fungi that infect the insect pest S. Frugiperda. The results of the pathogenicity test and macroscopic and microscopic identification showed that the characteristics of the isolates refer to the fungus Aspergillus flavus.
Co-Authors - Hamid Agung, I Dewa Agung Putra AGUS PUTRA SEDANA Ali Nurmasyah Americo Alves Brito Anak Agung Ayu Agung Sri Sunari Anak Agung Sagung Kendran ANANDA RIZKI NURANI Anu Watu, Getrud Novia ARIYANTA, I PUTU BAWA Chiharu Hongo Damastra, Garda Bagus Desak Putu Sudarmini Devi, Komang Saraswati Devi, Putu Shinta DEWA AYU DIAH PUSPITASARI Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga DINA RAHMAWATI Dinarkaya, Shah Mahapati Dwi Martiningsia Dwi Martiningsia DWI SUGIARTA DWI WIDANINGSIH Eka Wijayanti, Febri G.A.M.K. Dewi GARGITA, I WAYAN DIKSA Gede Suastika Gede Suastika GREG LUTHER GREGORY C. LUTHER Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Gusti Ngurah Alit Susanta Wirya GUSTI NGURAH GEDE DHARMA PUTRA PUTRA Hamid, - Hanifah, Wafa’ Nur Harianja, Shara Yulita Hartha , I Komang Gede Suweca I DEWA MADE PUTRA WIRATAMA I Dewa Nyoman Nyana I DEWA PUTU SINGARSA I DEWA PUTU SINGARSA, I DEWA PUTU I G. R. M. TEMAJA I G. Wijana I GEDE PUTU WIRAWAN I GEDE SILA ADNYANA I Gst Agung Putu Raka Agung I GUSTI AYU DEVI VALENIA SARI I Gusti Ngurah Bagus I Gusti Ngurah Kade Mahardika I Ketut Mangku Budiasa I KETUT PURNA YASA I KETUT SUMIARTHA I Komang Candra Giri Prayoga I KOMANG VIDIA DHARMA TARO PUTRA I Made Arimbawa I Made Dira Swantara I Made Sudana I MADE SUDARMA I Made Sukewijaya I MADE SUPARTHA UTAMA I MADE WINANTARA I MADE YESTA SANTIATMA I NYOMAN DARMA YASA I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I NYOMAN WIJAYA I PUTU BAWA ARIYANTA I PUTU DHARMA I PUTU WIRYA SUPUTRA I Wayan Arta Wijaya I WAYAN RUSMAN I Wayan Suardana I Wayan Supartha I Wayan Susila I.A.P. Apsari I.B.K. Suardana Ida Ayu Pasti Apsari IDA BAGUS GDE PRANATAYANA Ida Bagus Kade Suardana JOKO MARIYONO JOKO MARIYONO KETUT AYU YULIADHI Klett, Katrina Listihani, Listihani Luciana Delavega LUTFI SURYAWAN M SUDANA Made Getas Pudak Wangi MADE MIKA MEGA ASTUTHI Made Mika Mega Astuthi Made Satya Andrayuga MARIA ULFA ELLA ELLA Masahiro Shishido Maulinda, Restiana MOCH BINTANG RAMADHAN NI KADEK NINA ARI SUCI Ni Kadek Nita Karlina Astriyani Ni Luh Gede Sudaryati NI LUH WAHYU SUTARINI, NI LUH WAHYU NI MADE DWI ANTARI Ni Made Dwi Desiyanti Ni Made Puspawati NI MADE SAVITA RASJMAN NI MADE WINDA UTARI NI NENGAH DARMIATI Ni Putu Merthaningsih Ni Putu Noviyanti Ni Wayan Korniasih NI WAYAN SUNITI NYOMAN SEMADI ANTARA Putra, I Gusti Putu Semara Putu Perdana Kusuma Wiguna R. SRINIVASAN RAMADHAN, MOCH BINTANG S.K. Widyastuti SANJAYA, I GUSTI NGURAH PRABU WIRA Selangga, Dewa Gede Wiryangga SONIA ASHA HASARI Sri Kayati Widyastuti SUGIARTA, DWI Sumartayasa, I Wayan Andi SUNARI, ANAK AGUNG AYU AGUNG SRI Suputra , I Putu Wirya SUPUTRA, I PUTU WIRYA Syahbana , Raditya Darmawan TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Yuliadhi , Ketut Ayu Zhao, Tiejun