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Journal : Jurnal Biologi Tropis

ANALISIS HUBUNGAN PANJANG BERAT PADA IKAN HERMAPHRODIT : KERAPU SUNU (Plectropomus leopardus) DAN KERAPU MACAN (Epinephelus fuscoguttatus) Haris Setiawan; Achmad Fahrudin; Mohammad Mukhlis Kamal
Jurnal Biologi Tropis Vol. 19 No. 2 (2019): Juli - Desember
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (427.563 KB) | DOI: 10.29303/jbt.v19i2.1162

Abstract

Abstrak : Penelitian ini bertujuan untuk mengeksplorasi hubungan panjang berat dua spesies ikan kerapu hermafrodit, yaitu kerapu sunu (Plectropomus leopardus) dan kerapu macan (Ephinephelus fuscoguttatus). Sebanyak 1.263 individu ikan yang terdiri dari 609 dan 654 spesies pertama dan kedua, masing-masing dikumpulkan dari perairan Wangi-Wangi dan Karang Kaledupa Taman Nasional Wakatobi selama September 2018 hingga Februari 2019. Ikan dikumpulkan secara acak yang merupakan hasil tangkapan pancing  dan tombak. Hasil  kedua spesies menunjukkan pola pertumbuhan alometrik (p <0,01 untuk keduanya) di mana nilai b masing-masing adalah 2,46 dan 2,73. Berdasarkan koefisien korelasi (R2), ditemukan bahwa variasi berat mampu menjelaskan 90% lebih panjang tubuh ikan. Berdasarkan panjang ikan saat tertangkap dan panjang pertama kali matang gonad, ditemukan bahwa betina P. leopardus sebagian besar ditangkap sebelum matang gonad (> 70%), sedangkan E. fuscoguttatus untuk kategori yang sama berada pada persentase yang lebih rendah (<50%). Langkah-langkah pengelolaan pada spesies hermafrodit dapat difokuskan pada proporsionalitas antara betina dan jantan terhadap keberhasilan reproduksi.Kata Kunci : Panjang Berat, E. fuscoguttatus, P. leopardusAbstract : The present research was aimed to explore the length-weight relationship-based growth of two hermaphrodite groupers, leopard coral grouper (Plectropomus leopardus) and tiger grouper (Ephinephelus fuscoguttatus). A total of 1,263 individual fishes comprising 609 and 654 individuals of these two species respectively, were collected from Wangi-Wangi and Kaledupa reef in Wakatobi National Park from September 2018 to February 2019. Fish were randomly sampled from catches that used hook-and-lines and spears. The results found that both species follow allometric growth patterns (p<0,01 for both) of which b values were, respectively, 2,46 and 2,73. Based on coefficient correlation (R2), it was found that weight variability is able to explain more than 90% of length. By comparing length at catch and length at first maturity, it was found that females of P. leopardus were largely caught at pre-mature stage (>70%), while for E. fuscoguttatus <50% of females were pre-mature. We suggest that management measures for hermaphrodite species should focus on the proportionality between females and males towards their reproductive success.Keywords : Length-weight relationship, E. fuscoguttatus, P. leopardus
AUTENTIKASI SPESIES IKAN KERAPU BERDASARKAN MARKA GEN MT-COI DARI PERAIRAN PEUKAN BADA, ACEH Mohammad Mukhlis Kamal; Agus Alim Hakim; Nurlisa Alias Butet; Yulia Fitrianingsih; Rika Astuti
Jurnal Biologi Tropis Vol. 19 No. 2 (2019): Juli - Desember
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.161 KB) | DOI: 10.29303/jbt.v19i2.1245

Abstract

Abstrak : Variasi bentuk dan pola pewarnaan tubuh ikan kerapu (Famili Serranidae) sangat variatif, sehingga pengenalan spesies secara morfologis sering tidak akurat. Penelitian ini bertujuan untuk mengautentikasi ikan kerapu dengan menggunakan marka gen COI. Contoh ikan kerapu yang diamati berjumlah 29 ekor yang dikumpulkan dari tiga tempat pendaratan ikan di Perairan Peukan Bada, Propinsi Aceh. Secara karakter morfologis, ikan kerapu tersebut teridentifikasi lebih dari 8 spesies. Untuk analisis DNA, sebanyak 30 mg daging sirip dari setiap ikan contoh diambil untuk dilakukan isolasi dan ekstraksi DNA, kemudian visualisasi elektroforesis dan fragmentasi DNA gen COI dengan metode PCR-sekuensing. Setelah diekstraksi, diperoleh 20 sampel DNA yang tervisualisasi dengan baik, yang dari jumlah tersebut terdapat 16 sampel dapat diamplifikasi. Hasilnya menunjukkan terdapat 6 spesies yang terautentikasi. Kelompok pertama adalah Variola albimarginata, Cephalopholis urodeta, dan C. sexmaculata dengan tingkat kemiripan ≥ 97%. Berikutnya C. boenak, Epinephelus merra, dan Scolopsis vosmeri tingkat kemiripannya ≤ 97%. Bila dibandingkan hasil autentikasi DNA, hasil penelitian menunjukkan bahwa 13 sampel atau > 80% tidak teridentifikasi dengan benar secara morfologis. Berdasarkan jarak genetik, pohon filogeni membentuk 2 clade antara Serranidae dan Nemipteridae. Hasil penelitian menunjukkan bahwa penggunaan marka gen COI sangat efektif untuk autentikasi spesies yang dapat dijadikan sebagai instrumen dalam pemanfaatan dan pengelolaan ikan kerapu.Kata kunci : kerapu, variasi morfologi, gen MT-COI, autentikasi.Abstract : The groupers (family Serranidae) show high variability both in body shapes and coloration leads to highly morphological-based misidentification. The research was aimed in autenthication of the grouper species using MT-COI gene. A total of 29 grouper fishes were collected from three fish landing sites of Peukan Bada, Aceh Province. These fishes were morphologically identified from which more than 8 species were obtained. A 30 mg of the fin meat of each sample was taken for DNA extraction, isolation, electrophoresis visualization, and DNA fragmentation of COI gene using PCR-sequenching.  There were 20 DNA samples was clearly visualized of which 16 has been proceeded for amplification. The results showed that V. albimarginata, C. urodeta, and C. sexmaculata showed ≥ 97% similarity, whereas C. boenak, E. merra, dan S. vosmeri with ≤ 97% similarity. Based on phylogenetic tree analysis there was 2 clearly different clades separating family of Serranidae and Nemipteridae. The use of MT-COI gene was effective and accurate tool in species authentication which could be used as an instrument for utilization and management of the grouper species.Keywords : groupers, morphological variation, MT-COI gene, autenthication.
LENGTH AT FIRST MATURITY, SPAWNING TIME, AND REPRODUCTIVE OUTPUT IN THE FEMALES OF HAMILTON’S ANCHOVY (THRYSSA HAMILTONII GRAY, 1835) Mohammad Mukhlis Kamal; Yunizar Ernawati; Nina Nurmalia Dewi
Jurnal Biologi Tropis Vol. 20 No. 1 (2020): Januari - April
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.317 KB) | DOI: 10.29303/jbt.v20i1.1623

Abstract

Abstract: The Hamilton’s anchovy (Thryssa hamiltonii Gray, 1835) is one of main target species in Indonesian small pelagic fishery. This engraulids member is characterized by short lifespan and fast reproductive cycle. The research aims were to explore the reproduction aspects of the females Hamilton’s anchovy in terms of changes in length at first maturity, spawning seasons, and fecundity as well as egg diameter as reproductive output. Fish was collected weekly during 2009 at fish landing site at Gebang Mekar, Cirebon. The fish was length measured, after which ovaries staged for gonadal maturity stage (GMS), fecundity, and eggs diameter. Length frequency data was all linked to this reproductive aspects. The results showed that Lm was 171.5 ± 1.62 mm, comparable to annual calculation of 171.05 ± 0.01 mm, Lc largely tend to smaller than Lm which indicated to unsustainable fishing. Fish spawns monthly with fecundity range between 3,456 – 15,843 (8,261 ± 2,776 eggs), it might be categorized as high fecundity fish relative to its body size. The distribution of eggs diameter showed 2-3 modus, i.e. they are partial spawner fish. The discrepancies in reproductive ouput is suggested to be influenced by seasons which characterized by different environmental conditions. Keywords: Hamilton’s anchovy, females, length, spawning, reproductive output
Sunfish’s (Mola spp.) Habitat Characteristics on their Appearance at Dive Tourism Depths in Nusa Penida Waters, Bali Dewi SLKT; Mohammad Mukhlis Kamal; T Tarsidin; G Yulianto
Jurnal Biologi Tropis Vol. 21 No. 1 (2021): Januari - April
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i1.2442

Abstract

The sunfish or mola fish (Mola spp.) is one of marine tourism’ icon in Nusa Penida waters where they appear seasonally. This study was aimed to observe the habitat characteristics of mola fish at the depths of diving tourism where they are visible. The research was conducted from July to December 2019 using survey and descriptive methods to obtain information of mola fish animal linked to habitat conditions. On its appearance data on depths, temperatures, currents, and the presence of symbiotic fishes were observed directly assisted by dive center operators. By comparing the morphological characteristics shown photographically, it is assumed that mola fish in study area is composed by two species, M. alexandrini and M. mola. The highest individual record was in September and decreasing along the incoming months. Mola fish prefers calm deeper water with lower temperatures coincides with the presence of cleaning reef fishes. 
Heavy metal content of Pb and Cd in bandik grouper (Cephalopholis boenak) in Banten Bay, Indonesia Endang Juniardi; Sulistiono Sulistiono; Sigid Hariyadi; Mohammad Mukhlis Kamal
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.2830

Abstract

Banten Bay is a developing area that is used by various activities such as fishing ports, industry and hotels. Many activities in the coastal areas of Serang City and Cilegon Regency have a serious impact on the quality of waters and fishery resources along Banten Bay. This study aims to analyze and determine maximum tolerance limit for bandik grouper (C. boenak) meat and gills which are safe for consumption by adults and children. The study was conducted at six stations, namely Panjang Island and Pamujaan Besar Island during the period of May, June and July 2019. Measurement of heavy metal content on meat and gills carried out using Atomic Absorption Spectrophotometric (AAS). The content of Pb and Cd in water fluctuates. The metal content of Pb and Cd in the meat and gills of bandik grouper has exceeded the quality standards set by BPOM and FAO/WHO, and is classified as polluted mild to moderate Pb bioconcentration factor values in grouper meat ranged from 6.78-72.00 and Pb bioconcentration in grouper gills 32.86-762.61. The maximum weight of grouper meat that can be tolerated for consumption for adults is 3,5 kg/week and children 1.0 kg/week.
Co-Authors . Zairion Achmad Fahrudin Achmad Fahrudin Achmad Fahrudin Achmad Fahrudin Achmad Fahrudin Aditiyawan Ahmad Agoes Mardiono Jacoeb Agus Alim Hakim Agus Atmadipoera Agus Oman Sudrajat Ahyar Pulungan Ali Mashar Ali Suman Ali Suman Ali Suman Aliati Iswantari Apriana Vinasyiam Ariane Koch-Larrouy Arif Miftahul Aziz, Arif Miftahul Arif Wibowo Arif Wibowo Ario Damar Arlita, Kriswidya Ayu, Inna Puspa Beatrix M. Rehatta Beginer Subhan Booth, Hollie Bosman, Ofan Cahyanti, Wahyulia Dade Jubaedah Dade Jubaedah Darmawan, Aldo Dawi, Mario Raja Sani Deni Irawan, Deni Deni Radona, Deni Dewi SLKT Dewi, Nina Nurmalia Dwi Yuni Wulandari Efin Muttaqin Eko Prianto, Eko Elok Faiqoh Enan M Adiwilaga Endah Sri Rahayu Endang Juniardi Endi Setiadi Kartamihardja Endi Setiadi Kartamihardja Fera Permata Putri, Fera Permata Forcep Rio Indaryanto G Yulianto Gadis Sri Haryani Gadis Sri Haryani Gatot Yulianto Gilang Rusrita Aida Grin Tommy Panggabean Hafizh, Ayesha Harahap, Wini Aafini J Haris Setiawan Helmy Akbar Hendrik Sombo I Nyoman Suyasa Iis Diatin Iman Wahyudin Irin Iriana Kusmini, Irin Iriana Ismudi Muchsin Ismudi Muchsin Ismudi Muchsin Iwan Hasri Iwan Hasri, Iwan Jabbar, Meuthia Aula Jifi Abu Ammar Jojo Subagja Kadarwan Soewardi Kiagus Abdul Azis Kristanto, Anang Hari Kurniawan Kurniawan La Ode Syahlan S Sagala Larasati, Aulia Nur Larashati, Sekar Luky Adrianto Luky Adrianto Ma'mun Ansori Mala Nurilmala Mennofatria Boer Mia Setiawati Mirza Dikari Kusrini Mohamad Jakaria Muhammad Arif Nasution Muhammad Nizar Muhammad Nizar Muhammad Nizar Nabillah, Firja Hasanah NEVIATY PUTRI ZAMANI Niken Tunjung Murti Pratiwi Nugroho, Dwiyoga Nurasiah Riza Nurlisa Alias Butet Nurseptiani, Sifa Okta Simon Oktavia, Nia Otong Zenal Arifin Prakoso, Vitas Atmadi Pratiwi, Niken TM. Prihatiningsih Prihatiningsih Radityani, Fitri Afina Rahmat Kurnia Ramdayani, Kasturi Ridwan Affandi Ridwan Affandi RIDWAN AFFANDI Rika Astuti Risa Tiuria Rizsa Mustika Pertiwi Robin Robin, Robin Rohmah, Lailia Nur Ronny I. Wahju Rudhy Gustiano Rumagia, Faizal Sagala, La Ode Syahlan S Sakina, Edna Samir, Octavianto Sayaza, Mas Davino Shinta Pardede Sifa Nurseptiani Sigid Hariyadi Sigid Hariyadi Singgih Afifa Putra Siti Rahmawati Stuart J Campbell Sukmaraharja Tarigan Sulistiono Sulistiono Sulistiono Sulistiono Surjanto, Didiek Syarifah Nurdawaty T Tarsidin Taryono Taryono, Taryono Tatag Budiardi Thomas Hidayat Tri Hartono, Tjahjo Trie, Triyanto Triyanto Triyanto Triyanto Triyanto Triyanto, Triyanto Utama, Darma Wahidin, Nurhalis Widoretno, Mey Ristanti Wildan, Dudi Muhammad Y. Rahmah Yanti Sinaga Yulia Fitrianingsih Yunizar Ernawati Yunizar Ernawati Yusli Wardiatno Yuspita, Ni Luh Eta