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All Journal Jurnal Pangan dan Gizi Jurnal Sains dan Seni ITS Biopropal Industri QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Eureka Herba Indonesia JURNAL AGROINDUSTRI HALAL JPM-AKA Jurnal Ilmiah Pangan Halal Karimah Tauhid
Siti Nurhalimah
Food Science Study Program, Graduate Student, IPB University
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Social Sciences Other

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Journal : Biopropal Industri

 1 
AKTIVITAS ANTIOKSIDAN DARI METABOLIT SEKUNDER KAPANG ENDOFIT MANGROVE Aegiceras corniculatum Siti Nurhalimah; Siti Irma Rahmawati; Joko Hermanianto; Siti Nurjanah; Fauzia Nurul Izzati; Eris Septiana; Fauzy Rachman; Bustanussalam Bustanussalam; Yatri Hapsari; Partomuan Simanjuntak; Masteria Yunovilsa Putra
Biopropal Industri Vol 12, No 1 (2021)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36974/jbi.v12i1.6539

Abstract

ABSTRACTMangrove endophytic fungi potentially produce secondary metabolites such as antioxidant. Antioxidants are compounds that are widely applied in the industry as an ingredient in the manufacture of products in the food and health sector. In this study, we investigated the antioxidant  potential of secondary metabolites from fungal endophytic of mangrove species Aegiceras corniculatum. A total of eight endophytic fungi were successfully isolated, two isolates from leaf, three isolates from fruit and three isolates from twig.  Between the isolates obtained, only one isolated was active as the antioxidant with IC50 value 19.28 µL/mL eventhough still lower than the standard ascorbic acid (6.08 µL/mL). The results of chemical identification using GC-MS showed several chemical compounds that have antioxidant activity including phenol,3,5-bis(1,1-dimethylethyl), hexadecanoic acid, hexadecanoic acid methyl ester, malic acid, N-aminopyrrolidine,  9-octadecanoic acid, methyl ester (E), benzeneethanol, 4-hydroxy, 1,2-benzenedicarboxylic acid, d-tyrosine, bis(2-methylpropyl) ester 1-nonadecene dan heneicosane.  The selected fungal endophytic isolated were identified using molecular Internal Transcribed Spacer (ITS) marker and has a high taxonomy similarity with Microdochium sp.Keywords: Aegiceras corniculatum, antioxidant, endophytic fungi, mangrove, Microdochium sp ABSTRAKKapang endofit dari mangrove memiliki potensi dalam menghasilkan metabolit sekunder seperti antioksidan. Antioksidan merupakan senyawa yang banyak diaplikasikan dalam industri sebagai  bahan untuk pembuatan produk di bidang pangan maupun kesehatan. Penelitian ini bertujuan untuk  mengetahui potensi antioksidan kapang endofit dari mangrove species Aegiceras  corniculatum. Sebanyak 8 isolat kapang endofit berhasil diisolasi, yaitu 2 isolat berasal dari daun, 3 isolat berasal dari buah dan 3 isolat berasal dari ranting.  Di antara 8 isolat tersebut, hanya 1 isolat yang dihanyakan aktif sebagai antioksidan dengan nilai IC50  sebesar 19,28 µL/mL, meskipun nilai ini masih lebih rendah dibandingkan vitamin C yaitu sebesar 6,08 µL/mL. Hasil identifikasi kimia dengan menggunakan GC-MS menunjukkan beberapa senyawa kimia yang memiliki aktivitas aktioksidan di antaranya phenol,3,5-bis(1,1-dimethylethyl), hexadecanoic acid, hexadecanoic acid methyl ester, malic acid, N-aminopyrrolidine,  9-octadecanoic acid, methyl ester (E), benzeneethanol, 4-hydroxy, 1,2-benzenedicarboxylic acid, d-tyrosine, bis(2-methylpropyl) ester 1-nonadecene dan heneicosane. Isolat kapang endofit diidentifikasi dengan penanda Internal Transcribed Spacer (ITS) dan memiliki kemiripan tertinggi dengan Microdochium sp.Kata kunci: Aegiceras corniculatum, antioksidan, kapang endofit, mangrove, Microdochium sp
Co-Authors , Sunawan Afiani, Nur Amanda, Ressa Ananda, Dea Dwi Anisyah, Yuni Nur Arbayani, Arbayani Ariestyani, Imelda Arti Hastuti Azzahra, Azzahra Bustanussalam Bustanussalam Delfitriani Depita Nurapni Distya Riski Hapsari Dwi Aryanti Nur’utami Eeb Saifullah Fatah Elva Stiawan Eris Septiana Fadhilah, Syifa Fatma, Reyna Fauzi, Rizki Fauzia Nurul Izzati Fauzy Rachman Febriansyah, Ilham Fina Uzwatania Fitrilia, Tiana Guna Wibawa, Sultan Harefa, Harefa Himyatul Hidayah Husna Mahendra Putra Intan Kusumaningrum Intan Permatasari Irahmah, Siti Izzatu Rahmah, Afifah Joko Hermanianto Kartawiria, Rifky Fauzi Althafry Kharyani, R. Aldini Kurniawan, M. Fakih Lia Amalia Liska Badriansyah maharani, Azmi Marhamah, Irma Siti Masteria Yunovilsa Putra Melati, Risma Mindawati, Erisa Mirna Febi Alfisyah MS, M. Adrian Rezki Anggara Muhammad Taufik Muhibuddin, Anton Muthiatulmillah, Siti Nafisa, Azka Sayyida Navis Febiansyah Novia Putri Dewi Nuraeni, Dinda Nurcahali, Fani Nurlaela, R. Siti Nurlita, Nurlita Nursyawal Nacing P, Alsa Denta Partomuan Simanjuntak Permatahati, Ghina Sri Pratami, Aprilia Qisthina, Dalilah R, Fairuz Irdina Rabiatul Arabiah Raden Siti Nurlaela Rahmawati, Siti Irma Riski Hapsari , Distya Rizkia, Safa Rosy Hutami Rosyid Ridho Rudi Efendi Sadiyatul Islami Salsabila Nurul Izha Saniyya, Ashila Nasyadhiya sapanli, kastana Septian, Willyandi Septiani, Bunga Septiani, Nadia Siti Aminah Siti Aminah Siti Masithoh Siti Nurjanah Siti Solihat Sri Agustina, Sri Sri Nurhatika Sugandi, Sugandi T, Elsya Nahwan Tayon, Felix Titi Rohmayanti Wulandari, Fifi Yulia Yatri Hapsari Yoti, Agatha Dwi Yulia, Fifi Zahro, Halimah Az