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PUSPITA LISDIYANTI
Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Journal : Jurnal Riset Biologi dan Aplikasinya

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Beta-Glucosidase 1 (bgl1) Gene Analysis in Mutant and Wild-type of Penicillium sp. ID10-T065 Syafriana, Vilya; Nuswantara, Sukma; Mangunwardoyo, Wibowo; Lisdiyanti, Puspita
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 1 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n1.p1-8

Abstract

In the previous study, Penicillium sp. ID10-T065 has been mutated using ultraviolet (UV), ethyl methyl sulfonate (EMS) and the combination of UV-EMS to increase Beta-glucosidase (bgl) activity. There were three mutants selected, UV13 (UV mutant), EM31 (EMS mutant), and UM23 (UV-EMS mutant). This study examined the mutations in the bgl gene encoding (bgl1) as well as sequence differences between mutants and wild-type of Penicillium sp. ID10-T065. The gene analysis was performed by PCR amplification and sequencing of the bgl1 gene. The results of bgl1 gene sequences (600 bp) from mutants were aligned with the wild-type, it was discovered that there were base alterations from position 2025 to 2050. Mutant UV13 showed the highest base alterations (7 bases) which occurred at position 2027 (T?C); 2036 (T?G); 2040 (T?G); 2047 (G?C); and 2048-2050 (TTG?GGA). Mutant EM31 showed alterations in five bases at positions 2034 (G?A), 2036 (T?G), 2037 (G?C), 2044 (G?C), and 2047 (G?T). Mutant UM23 showed two base alterations at position 2025 (T?A) and 2037 (G?C). UV irradiation and EMS mutation resulted in transition and transversion DNA, whereas the combination of UV-EMS mutation resulted in transversion mutations. Base alterations in UV13 and EM31 mutants, causing missense and silent mutation, while in UM23 mutant only silent mutations occur. The bgl1 gene analysis showed that mutation using UV light was more effective than using EMS or a combination of UV-EMS in Penicillium sp. ID10-T065.
Co-Authors - Yopi Abd. Rasyid Syamsuri ADE ANDRIANI Adi Santoso Agustinus Robert Uria Ahmad Thontowi Akhmad Rivai ANDI UTAMA Andi Utama ANDO KATSUHIKO Apriliana, Pamella Ariani Hatmanti Ario Betha Juanssilfero Ashadi Sasongko Atikana, Akhirta Caniago, Asnany Caniago, Asnany Desriani Desriani Desriani Desriani Dewi Seswita Zilda dinihari indah kusumawati Dwi Wulandari Elvi Yetti Endang Saepudin, Endang Endang Sukara Erdiansyah Rezamela, Erdiansyah Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi GINA KARTINA Herman Suryadi JAKA WIDADA KATSUHIKO ANDO Krause, Janina Kusharyoto, Wien Kusharyoto, Wien Kusumawardhani, Dinihari Indah Kusumawati, Dinihari Indah Kusumawati, Dinihari Indah Lina Elfita Lina Elfita LINDA SUKMARINI M. Iqbal Prawira-Atmaja, M. Iqbal Mangunwidjaja, Wibowo MARIA BINTANG Maulidia, Faiqoh Nur Meliah, Siti Meliah, Siti Mia Kusmiati Miranti Nurindah Sari, Miranti Nurindah MISA OTOGURO Mochammad Imron Awalludin Mulyorini Rahayuningsih Nadhifah, Hana NANIK RAHMANI Nanik Rahmani Neneng Hasanah Neng Herawati, Neng Ni Luh Putu Vidya Paramita Nurul Fitri Sari Nuryana, Isa Prasetyoputri, Anggia Pratama, Rendy Eka Prayoga, Muhammad Bagas Putra, Filemon Jalu Nusantara Putra, Masteria Yunovilsa Putri, Ade Lia Putri, Ade Lia Restu Wulansari, Restu Riyona Desvy Pratiwi, Riyona Desvy ROHMATUSSOLIHAT ROHMATUSSOLIHAT RONI RIDWAN Seprianto, Seprianto Shanti Ratnakomala SHANTI RATNAKOMALA Shanti Ratnakomala Siti Meliah Subagus Wahyuono Suharsono Suharsono Sukma Nuswantara Susanti, Agustina Eko Syafriana, Vilya Tisnadjaja, Djadjat Tisnadjaja, Djadjat Titik Nuryastuti TOMOHIKO TAMURA Toto Iswanto TRI RATNA SULISTIYANI Triana Hertiani Trisanti Anindyawati, Trisanti Tutik Murniasih Ukhradia Maharaniq Safira Untari, Febriana WIBOWO MANGUNWARDOYO Yana Rubiyana, Yana Yantyati Widyastuti Yopi Y YOPI YOPI Yopi Yopi Yopi, YULIN LESTARI Yusro Nuri Fawzya