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Journal : Pro-Life

AKTIVITAS PROTEOLITIK BAKTERI LOKAL YANG DI ISOLASI DARI TEMPAT PENANGKARAN BUAYA Jendri Mamangkey; Dwi Suryanto
Jurnal Pro-Life Vol. 8 No. 2 (2021): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Indonesia has an ecological diversity which can be used as significantly potential habitat for local bacteria. One of such environments is crocodile breeding where research on proteolytic bacteria is still minimal. Proteolytic bacteria are a type of bacteria which can produce protease enzymes. This research was performed aiming to find selected proteolytic bacteria that can produce protease enzymes. Pour plate method using skim milk agar media was employed to isolate the bacteria. Bacteria isolates were selected based on qualitative and quantitative tests. Current research results discovered 6 bacteria isolates which had proteolytic ability after the qualitative test. Among those 6 isolates, 3 (FB2, THB1b, and THB6a) were able to hydrolyze skim milk media with the fastest incubation time of 18 hours. Meanwhile, based on quantitative test, 1 isolate produced the highest protease activity, which is FB2 isolate with 958 U/mL having a total of 12.05 cells/mL. Based on this research report, FB2 isolate collected from crocodile feces (FB) has the potential to be utilized in protease enzymes application in the environment.
Aktivitas Mikrobiologis Endofit dari Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) Jendri Mamangkey; Lukas Pardosi; Riska Septia Wahyuningtyas
Jurnal Pro-Life Vol. 9 No. 1 (2022): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Endophytic bacteria and fungi inhibite the tisues of living plant. Both of them are significant components of plant microecosystems. Current research was performed to explain several microbiological activities (production of extracellular enzymes, antioxidants, and antibacterials) of endophytic bacteria and fungi from binahong leaves extract (Anredera cordifolia (Ten.) Steenis). In this case, the research was conducted by applying a review of several literature reviews published online on Google Scholar and several journals listed on the ScienceDirect site. Based on the results obtained from the research reports from several scientific journal sources, there are four types of extracellular enzymes produced by the endophytic bacteria of the Anredera cordifolia plant, including proteinase, amylase, esterase, and cellulase. In addition, the endophytic bacteria of Pseudomonas aeruginosa from the leaves extract of Anredera cordifolia has antioxidant activity, which is volatile compounds including cis-9-Octadecenoic acid, 2-dodecenoic acid, and 9 octadecenamide produced by endophytic bacteria of Pseudomonas sp. Furthermore, the endophytic bacteria isolated from Anredera cordifolia leaves extract inhibits the growth of E. coli ATCC 35216 and S. aureus ATCC 29523. Meanwhile, the endophytic fungi from Anredera cordifolia leaves extract inhibite the growth of E.coli and S. aureus bacteria. Therefore, understanding the relationship between bacteria and endophytic fungi from the leaves extract of binahong (Anredera cordifolia (Ten.) Steenis) is essential. This knowledge can be utilized and applied well for the future production of higher quality and more medicinal plants from binahong plant (Anredera cordifolia (Ten.) Steenis).
Gambaran Kejadian Ikutan Pasca Imunisasi Covid-19 pada Mahasiswa Fakultas Kedokteran UKI Penyintas Covid-19 dan Non Penyintas Covid-19 Desy Ria Simanjuntak; Jendri Mamangkey; Nive Winda Sari Lumban Tungkup; Anggi Izdihar Mahaswari; Talitha Amalia; Bethaniel Roy Matthew
Jurnal Pro-Life Vol. 9 No. 1 (2022): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Adverse Events Following Immunization (AEFI) is defined as 'any adverse medical event that occurs after immunization, but which does not necessarily have a cause-and-effect relationship with vaccine use. Research conducted by krammer, et al said that vaccine recipients with pre-existing immunity (seropositive) experienced systemic side effects with a much higher frequency than antibody-nave vaccines. UKI Medical Faculty student environment class 2018 and 2019. The research design used is descriptive research with a sample of primary data taken using google form as a media for filling out questionnaires with a sampling method with simple random sampling , then the data is collected and will be processed with the Microsoft program excel. The number of respondents in this study amounted to 229 respondents, consisting of 115 students of class 2018 and 114 students of class 2019. The percentage of students who were non-survivors of COVID-19 was 77.73% and survivors of COVID-19 was 27.73%. Types of vaccines 1,2, and 3 received by the two groups included Sinovac, Astrazeneca, Moderna, and Pfizer. Symptoms of AEFI in non-survivors of COVID-19 are dominated by local symptoms in the form of pain at the injection site (vaccine 1; 88.3%, vaccine 2: 92.6%, vaccine 3: 93.8%), followed by other systemic symptoms such as myalgia., fever, and headache (vaccine 1: 11.7%, vaccine 2: 7.4%, vaccine 3: 6.2%). Symptoms of AEFI in COVID-19 survivors are dominated by local symptoms in the form of pain at the injection site (vaccine 1; 69.05%, vaccine 2: 93.55%, in vaccines 3 respondents experienced mixed local and systemic symptoms. Adverse Event Following Immunization (AEFI) which dominated in both groups in the form of local symptoms such as pain at the injection site, followed by systemic symptoms such as fever, myalgia, and headache Keywords: COVID-19, COVID-19 Vaccine, Adverse Events Following Immunization (AEFI)
Gambaran Pedagogical Content Knowledge (PCK) pada Calon Guru Biologi Di Universitas Kristen Indonesia Riska Septia Wahyuningtyas; Janed Lauren Soplantila; Jendri Mamangkey
Jurnal Pro-Life Vol. 9 No. 1 (2022): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This research aimed to determine the ability level of Pedagogical Content Knowledge (PCK) students of biology education at the Christian University of Indonesia. This research was a qualitative descriptive research. Data collection was carried out in two ways, namely by conducting a survey of respondents about pedagogic knowledge and content knowledge, then observing the learning implementation plan document which was assessed by two panelists. The population in this study were students of the Biology Education study program at the Christian University of Indonesia. The conclusion of this study was 1) The level of Pedagogical Content Knowledge (PCK) ability of biology education students was sufficient in terms of the results of written tests regarding Pedagogical Content Knowledge (PCK) knowledge, 2) Biology education students' Pedagogical Content Knowledge (PCK) ability level entry into the criteria is sufficient in terms of the results of the observation of the learning implementation plan (RPP) document
Co-Authors Abdillah, Latifah Ningrum Adinugraha, Fajar Adisti Ratnapuri Adrian Hartanto, Adrian Ai Hertati, Ai Akmaliyah, Rizna Amani, Febriyanti Nur Andika, Vrista Andry Pratama, Andry Angela, Ezra Anggi Izdihar Mahaswari Apon Zaenal Mustopa Arfa Yanti, Nur Ario Betha Juanssilfero Aritonang, Netty Arman Harahap Aryanti Pada Soa, Christina Asri Razad Aulia Septiani Baehaqi Bethaniel Roy Matthew Cengiz Kayacilar Chairunnisa, Sheila Chihombori, Tatenda Calvin Dana Briggs Dian Winarty Budadana, Christyani DWI SURYANTO Ekawati, Nurlaili Elida Octavia Evi Yulinda Tpoenifu Fatimah Frans Grovy Naibaho Gulo, Joice Elma Miranda Gusniarty Simanjuntak Gusti Ayu Kade Sutariati Hibatullah, Alnadia Yusriya Hutabarat, Anne Serafin Irawan, Herman Ivan Julius Rajagukguk Jajang Gumilar Janed Lauren Soplantila Janed Lauren Soplantila Jefrino Reis Pereira Joice Elma Miranda Gulo Joko Wahyono, Daniel Kartika Sari Dewi KIKI NURTJAHJA Lilis Suryaningsih Lomo, Jhoni Yakobus Lucas William Mendes Lukas Pardosi Mada Triandala Sibero Manguntungi, Baso Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Medyawanti Pane Mustopa, Apon Mutiara, Ilma Muttaqin, Salwa Zainum Napitupulu, Agnes Ester Napitupulu, Alexis Rachel Maria Rosauli Nelius Harefa Ni Made Yuliana Nitte, Tri Maharani Permata Nive Winda Sari Lumban Tungkup Novianti, Ela Nowlinda Kamuihkar Nur Amani, Febriyanti Nurfatwa, Maritsa Octavia, Elida Ode Adi Parman Rudia, La Ode Muhamad Iman Sulaiman, La Pereira, Jefrino Reis Pilidai Endorina Nadapdap Purba, Dian Pirma Natasari Putranto, Wendry Raden Mohamad Herdian Bhakti RATIH ASMANA NINGRUM Ratnapuri, Adisti Refli, Redoyan Ria Simanjuntak, Desy Riana, Febi Rifqiyah Nur Umami, Rifqiyah Nur Rika Indri Astuti Ripaldo Sihombing Riska Septia Wahyunigtyas Riska Septia Wahyuningtyas Rivaldi Sitinjak Rosdiana Virginia Rosenta Purba, Ika Sakka, Deni Frans Salwa Zainum Muttaqin Santhya Anaomi Tamansa Sekar Restuning Sembiring, Geskia Avikasari Septiani, Aulia Serafin, Anne Setiyadi Putranto, Wendry Siahaan, Frisca Olivia Sitinjak, Rivaldi Situmorang, Agnes Feronika Sri Swasthikawati, Sri SUNARTO Sunarto Sunarto Sunarto Sunarto Talitha Amalia Tesalonika Febriani Zebua Tpoenifu, Evi Yulinda Tresjia C Rakian Tri Maharani Permata Nitte Trinugroho, Joko P Wafaretta, Erona Wahyuningtyas, Riska Septia Wijaya, Hans Yenny Lisbeth Siahaan Yudi Purnomo Zaenal, Apon Zefanya Sipahelut