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Biotherapeutics and Antibiofilm Properties of Sponge-Associated Bacteria from Tanjung Tiram Mangroves, Southeast Sulawesi Mamangkey, Jendri; Sunarto, Sunarto; Fadjri, Corrina Lailatul; Irawan, Herman; Huda, Muhammad Komarul; Wibowo, Des Saputro; Mutiara, Ilma
BIOVALENTIA: Biological Research Journal Vol. 11 No. 2 (2025)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.11.2.2025.504

Abstract

 The search for new therapeutic agents has increasingly focused on bioactive compounds from sponge-associated bacteria. This study investigated bacterial symbionts of marine sponges collected from the mangrove ecosystem of Tanjung Tiram, Southeast Sulawesi, which have not been previously explored for their bioactive potential. Thirty bacterial isolates were screened in triplicate for antibacterial, antioxidant, antidiabetic, and antibiofilm activities. Antibacterial activity was evaluated using the disc diffusion method, antioxidant capacity by DPPH radical scavenging assay, α-glucosidase inhibition for antidiabetic potential, and antibiofilm activity by the crystal violet binding method. Several isolates showed notable bioactivities: strain Sp.8 produced the largest inhibition zone against Escherichia coli (15 mm), and Sp.14 showed the strongest inhibition against Staphylococcus aureus (23 mm). The most active antioxidant strains (Sp.14, Sp.15, Sp.13, and Sp.6) exhibited radical scavenging activity above 80%, while Sp.13, Sp.15, Sp.31, and Sp.37 showed high α-glucosidase inhibition (>74%). Strains Sp.38 and Sp.12 significantly reduced biofilm formation by E. coli and S. aureus, respectively. These findings reveal the untapped potential of sponge-associated marine bacteria from Tanjung Tiram as promising sources of bioactive metabolites for future drug discovery.
Effects of Augmented Reality Learning Media on Students’ Cognitive Learning Outcomes in Virus Topics Joice Elma Miranda Gulo; Wahyuningtyas, Riska Septia; Jendri Mamangkey
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 10 No 4 (2026): April
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v10i4.8090

Abstract

This study examines the effect of Augmented Reality (AR) media on students' cognitive learning outcomes on virus material in class X of SMA Negeri 51 Jakarta. The method used is a quasi-experimental method with a non-equivalent control group design, involving an experimental class with AR media and a control class with conventional media. Data were collected through pretests, posttests, and observation sheets to measure the implementation of learning and learning outcomes. The results of the analysis showed that AR media was implemented very well, with an average implementation of 97% during four meetings. The use of AR significantly improved students' cognitive learning outcomes in the domains of C1 (remembering), C2 (understanding), and C3 (applying) compared to conventional media. The results of the t-test showed a p value = 0.018 (p <0.05), with an average posttest of the experimental class of 73.08, higher than the control class of 65.63. AR media is effective in visualizing the abstract concept of viruses in improving students' understanding more deeply. The cognitive learning outcomes of students showed C1 = 82.14%, C2 = 81%, and C3 = 60%, higher than the control class. These results indicate that there is an influence of the use of Augmented reality media on students' cognitive learning outcomes on virus material at SMA Negeri 51 Jakarta.
Preliminary Study on Potential Bacterial Species Introducing Antibacterial Agent from Sponge in Unggeh Island Central Tapanuli, Indonesia Latifah Ningrum Abdillah; Dwi Suryanto; Kiki Nurtjahja; Jendri Mamangkey
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.8264

Abstract

Sponges have the potential to symbiont with bacteria that produce antibacterial compounds. This study was conducted to isolate and test the antibacterial activity of sponge symbiont bacteria. Bacterial isolates obtained were tested for antibacterial activity against Staphylococcus aureus, Bacillus subtilis and Escherichia coli using the Kirby-Bauer method. The identification results of both sponges have similarities with Clathria sp. and Hyrtios sp. symbiont bacteria obtained from both sponges as many as 16 isolates. Antibacterial activity testing had a weak inhibitory effect on isolates Sp1A, Sp2C, Sp2D and Sp2F, while Sp2J showed moderate inhibition against gram-negative and positive bacteria. The antibacterial activity of the whole supernatant of symbiont bacteria obtained the diameter of the inhibition zone with weak, medium and strong categories. Sp1A isolate supernatant was obtained, potentially able to fight Escherichia coli bacteria with a strong category as a representation of gram-negative bacteria and against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus) classified into the medium category. In conclusion, 16 symbiont bacterial supernatants have antibacterial activity mostly active against gram-negative bacteria Escherichia coli and gram-positive bacteria Bacillus subtilis. There are 6 isolates (Sp1A, Sp1B, Sp1E, Sp1F, Sp2D, Sp2F) active as antibacterial against Staphylococcus aureus with weak and moderate categories.
PELATIHAN PEMBUATAN TEMPE UNTUK PENGEMBANGAN LIFE SKILLS DAN KEMANDIRIAN PANGAN RSCA MAKASSAR Mamangkey, Jendri; Akbar, Muh. Syaiful; Wafaretta, Erona; Takdir, Nur Aisyah; Palennari, Muhiddin; Satirah, Siti Rana; Marsela, Alvioni; Amalia, Nurul Iffah; Annisa, Nur; Al Banna, Hasan
Jurnal Abdi Negeriku Vol 5, No 1 (2026): Jurnal Abdi Negeriku
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/jan.v5i1.84731

Abstract

Kesadaran masyarakat terhadap pangan fungsional mendorong perlunya edukasi mengenai teknologi pengolahan makanan yang bergizi dan higienis. Pengabdian kepada Masyarakat (PkM) ini bertujuan untuk mentransfer pengetahuan dan keterampilan teknis pembuatan tempe yang memenuhi standar keamanan pangan kepada para santri. Kegiatan dilaksanakan di Rumah Sehat Cancer Al-Qur’an (RSCA) Kota Makassar dengan melibatkan 12 santri sebagai peserta utama. Metode pelaksanaan dilakukan melalui pendekatan partisipatif dan praktik langsung yang meliputi sosialisasi teori, demonstrasi praktis, pendampingan langsung, serta evaluasi melalui kuesioner. Nilai rata-rata pre-test 49,00 dan nilai rata-rata post-test 89,25 dengan rata-rata peningkatan sebesar 40,25 dan persentase kelulusan sebesar 100%. Hasil pengukuran kepuasan peserta sebesar 66,7% menilai materi sangat baik dan 55,5% menyatakan cara penyampaian materi sangat jelas dan mudah dipahami. Para santri berhasil mempraktikkan tahapan pembuatan tempe secara mandiri, mulai dari pemilihan biji kedelai hingga proses fermentasi menjadi produk tempe. Kegiatan ini berhasil mewujudkan pengembangan kecakapan hidup (life skills) santri dalam bidang bioteknologi pangan sederhana. Melalui program ini, diharapkan para santri mampu mencapai kemandirian pangan internal serta memiliki jiwa kewirausahaan berbasis potensi lokal di lingkungan pesantren. Sinergi antara tim pengabdi dan lembaga mitra diharapkan dapat terus berkesinambungan melalui diseminasi materi biologi yang lebih variatif di masa depan.
Co-Authors Adinugraha, Fajar Adisti Ratnapuri Ai Hertati, Ai Akbar, Muh. Syaiful Akmaliyah, Rizna Al Banna, Hasan Amalia, Nurul Iffah Amani, Febriyanti Nur Andika, Vrista Andry Pratama, Andry Angela, Ezra Anggi Izdihar Mahaswari Apon Zaenal Mustopa Ario Betha Juanssilfero Aritonang, Netty Arman Harahap Aryanti Pada Soa, Christina Asri Razad Aulia Septiani Baehaqi Bethaniel Roy Matthew Cengiz Kayacilar Chairunnisa, Sheila Chihombori, Tatenda Calvin Dana Briggs DWI SURYANTO Dwi Suryanto Ekawati, Nurlaili Elida Octavia Evi Yulinda Tpoenifu Fadjri, Corrina Lailatul Fatimah Frans Grovy Naibaho Gulo, Joice Elma Miranda Gusniarty Simanjuntak Gusti Ayu Kade Sutariati Hibatullah, Alnadia Yusriya Huda Shalahudin Darusman Huda, Muhammad Komarul Hutabarat, Anne Serafin I wayan Teguh Wibawan Irawan, Herman Ivan Julius Rajagukguk Jajang Gumilar Janed Lauren Soplantila Janed Lauren Soplantila Jefrino Reis Pereira Joice Elma Miranda Gulo Joice Elma Miranda Gulo Joko Pamungkas Joko Wahyono, Daniel Kartika Sari Dewi KIKI NURTJAHJA Latifah Ningrum Abdillah Lilis Suryaningsih Lomo, Jhoni Yakobus Lucas William Mendes Lukas Pardosi Mada Triandala Sibero Manguntungi, Baso Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Marsela, Alvioni Medyawanti Pane Muhiddin Palennari Mustopa, Apon Mutiara, Ilma Muttaqin, Salwa Zainum Napitupulu, Agnes Ester Napitupulu, Alexis Rachel Maria Rosauli Nelius Harefa Nitte, Tri Maharani Permata Nive Winda Sari Lumban Tungkup Novianti, Ela Nowlinda Kamuihkar Nur Amani, Febriyanti Nur Annisa Nurfatwa, Maritsa Octavia, Elida Pereira, Jefrino Reis Pilidai Endorina Nadapdap Purba, Dian Pirma Natasari Putranto, Wendry Raden Mohamad Herdian Bhakti RATIH ASMANA NINGRUM Ratnapuri, Adisti Refli, Redoyan Ria Simanjuntak, Desy Riana, Febi Rifqiyah Nur Umami, Rifqiyah Nur Rika Indri Astuti Ripaldo Sihombing Riska Septia Wahyunigtyas Riska Septia Wahyuningtyas Rivaldi Sitinjak Rosdiana Virginia Rosenta Purba, Ika Sakka, Deni Frans Salwa Zainum Muttaqin Santhya Anaomi Tamansa Sari, Isti Kartika Satirah, Siti Rana Sembiring, Geskia Avikasari Septiani, Aulia Serafin, Anne Setiyadi Putranto, Wendry Siahaan, Frisca Olivia Sitinjak, Rivaldi Situmorang, Agnes Feronika Sri Swasthikawati, Sri SUNARTO Sunarto Sunarto Sunarto Sunarto Takdir, Nur Aisyah Talitha Amalia Tesalonika Febriani Zebua Tpoenifu, Evi Yulinda Tresjia C Rakian Tri Maharani Permata Nitte Trinugroho, Joko P Wafaretta, Erona Wahyuningtyas, Riska Septia Wibowo, Des Saputro Wijaya, Hans Yudi Purnomo Zaenal, Apon Zefanya Sipahelut