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All Journal HAYATI Journal of Biosciences Pro-Life Jurnal Penelitian Pendidikan IPA (JPPIPA) Bio-Lectura Reswara: Jurnal Pengabdian Kepada Masyarakat Spizaetus: Jurnal Biologi dan Pendidikan Biologi Indigenous Biologi International Journal Of Science, Technology & Management (IJSTM) Bioeduca : Journal of Biology Education Inteligensi : Jurnal Ilmu Pendidikan Jurnal ComunitA Servizio Bioscientist : Jurnal Ilmiah Biologi Oryza : Jurnal Pendidikan Biologi Journal of Natural Sciences BIOVALENTIA: Biological Research Journal International Journal of Health and Pharmaceutical (IJHP) Cocos Bio Jurnal Pengabdian Masyarakat SAPANGAMBEI MANOKTOK HITEI
Jendri Mamangkey
Universitas Kristen Indonesia
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Transportation Other

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EDUKASI DAN PELATIHAN PEMBUATAN PRODUK FRUIT YOGHURT BAGI SISWA SMA DHARMA SUCI DI JAKARTA UTARA Jendri Mamangkey; Marina Silalahi; Sunarto Sunarto; Riska Septia Wahyuningtyas; Fajar Adinugraha; Adisti Ratnapuri; Aulia Septiani; Joice Elma Miranda Gulo; Elida Octavia; Rivaldi Sitinjak; Tri Maharani Permata Nitte; Salwa Zainum Muttaqin; Jefrino Reis Pereira
RESWARA: Jurnal Pengabdian Kepada Masyarakat Vol 5, No 1 (2024)
Publisher : Universitas Dharmawangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46576/rjpkm.v5i1.3910

Abstract

Umumnya bakteri probiotik yang digunakan dalam fermentasi adalah Lactobacillus spp. dan Bifidobacterium spp.  Secara khusus, bakteri probiotik mampu mengasamkan bahan baku dengan cepat dengan memproduksi asam laktat dan beberapa senyawa penting lainnya. Teknologi ini penting diperkenalkan pada peserta didik, khususnya siswa/i di SMA Dharma Suci Jakarta, kegiatan pengabdian kepada masyarakat bertujuan untuk mengedukasi dan melatih para siswa membuat produk fruit yoghurt yang dapat menumbuhkan kreativitas baru terhadap pembuatan produk probiotik berbahan dasar wortel dan buah naga. Kegiatan pembuatan produk fruit yoghurt sebelumnya belum pernah dilakukan di SMA Dharma Suci Jakarta, pada akhirnya kegiatan PKM ini menjadi penting untuk mengenalkan bioteknologi sederhana produksi fruit yoghurt melibatkan bakteri probiotik yang multifungsi bagi kesehatan. Metode pelaksanaan PKM ini meliputi tahapan persiapan, tahapan pelatihan dan tahapan evaluasi. Hasil kegiatan PKM ini menambah informasi baru bagi siswa terutama pengetahuan yang berhubungan dengan aktivitas probiotik, prebiotik dan sinbiotik pada produk fruit yoghurt. Hasil kuesioner sebelum mengikuti edukasi/sosialisasi persentase siswa yang memahami probiotik hanya 57,1%, setelah mengikuti sosialisasi meningkat hingga 100%. Pemahaman ini didukung hasil kuesioner mengenai pertanyaan proses yang terjadi selama produksi fruit yoghurt, hasilnya 71,4% respon siswa menjawab fermentasi. Hasil pembuatan fruit yoghurt setelah fermentasi 24 jam berhasil terbentuk produk, berdasarkan hasil survei menurut siswa fruit yoghurt didominasi rasa manis dan asam. Ciri ini mengindikasikan produk fruit yoghurt berbahan dasar buah naga dan wortel dikategorikan baik
Pengaruh Limbah Organik Terhadap Pertumbuhan Kangkung Darat (Ipomoea reptans Poir) Dan Pemanfaatannya Sebagai Bahan Ajar Rosdiana Virginia; Riska Septia Wahyunigtyas; Marina Silalahi; Jendri Mamangkey
ORYZA ( JURNAL PENDIDIKAN BIOLOGI ) Vol 12 No 2 (2023): ORYZA: Jurnal Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi STKIP Bima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33627/oz.v2i2.1337

Abstract

Penelitian tahap pertama dilakukan untuk mengetahui pengaruh pupuk campuran limbah air beras dan kulit wortel terhadap pertumbuhan panjang batang, jumlah daun dan panjang akar tanaman kangkung darat. Metode yang digunakan yaitu eksperimen dengan Rancangan Acak Lengkap (RAL) yang terdiri atas 4 perlakuan dengan 6 kali ulangan. Adapun hasil penelitian ini menunjukkan bahwa pupuk limbah campuran air beras dan kulit wortel memberikan pengaruh terhadap pertumbuhan jumlah daun kangkung darat karena nilai sig. < 0,05, sedangkan yang lainnya tidak ada pengaruh karena nilai sig. > 0,05. Penelitian tahap kedua dilakukan untuk mengetahui bahan ajar yang dihasilkan. Metode yang digunakan yaitu pengembangan dengan model ADDIE yang terdiri atas 5 tahapan, yaitu analisis, perancangan, pengembangan, implementasi dan evaluasi. Namun dalam penelitian ini, hanya sampai tahap pengembangan. Adapun hasil penelitian ini menunjukkan bahwa LKPD yang dikembangkan sudah layak untuk digunakan oleh siswa/i kelas 12 SMA pada materi pertumbuhan dan perkembangan tumbuhan karena persentase skornya berada diantara 62,50% - 81,25%, baik dari segi materi, bahasa maupun media.
Purification and Characterization of Rennin Like Protease from Enterococcus faecium 1.15 Isolated from Indonesia Fermented Bakasam Putranto, Wendry; Suryaningsih, Lilis; Zaenal, Apon; Mamangkey, Jendri
International Journal of Science, Technology & Management Vol. 4 No. 4 (2023): July 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i4.900

Abstract

The protease enzyme produced by Lactic Acid Bacteria (LAB) with characteristics similar to rennin is called Rennin Like Protease (RLP). The purpose of the study was to obtain information on the biochemical characteristics of RLP produced by Enterococcus faecium 1.15 as a basis for the utilization of the enzyme. The first stage of the study was observation of the growth curves and the potential of the isolates to produce RLP, biochemical characterization. Rennin Like Protease (RLP) produced by Enterococcus faecium 1.15 which has been purified using Sephadex G-50 has a molecular weight of 42 kDa with biochemical characters, with an optimum temperature of 40 ? and pH of 4.5. The comparison between MCA/PA with a value of 3.74 which is close to the MCA value of Calf Rennet is 1.
Production Of Antimicrobial And Bioactive Peptides From Bakasam Using Enterococcus Faecium 1.15 As A Starter Setiyadi Putranto, Wendry; Gumilar, Jajang; Wulandari, Eka; Pratama, Andry; Mamangkey, Jendri
International Journal of Science, Technology & Management Vol. 4 No. 6 (2023): November 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i6.982

Abstract

The Development Of Traditional Fermented Food Products Into Functional Food, Utilizing Indigenous Microorganisms, Represents A Novel Innovation. Lactic Acid Bacteria (Lab), Which Are Abundantly And Readily Isolated From Native Fermented Foods, Hold Promise For The Exploration Of Local Lab Strains – A Strategic Step Towards The Development Of ProbioticBased Foods In Indonesia. Enterococcus Faecium 1.15 Isolated From Bakasam Beef, Has Demonstrated The Capability To Produce Extracellular Proteases (4.83 U/Mg) And Displayed Inhibitory Effects Against Pathogenic Bacteria. The Production Of Bioactive Peptides, With A Size Less Than 30 Kda, Could Be Achieved Through Bakasam Fermentation Using E. Faecium 1.15 As A Starter For A Period Of 7 Days. This Study Promotes An Opportunity To Harness Local Microorganisms, Particularly Lab Strains In The Utilization Of Bioactive Peptides As Well As Enhancing The Nutritional Value Of Traditional Fermented Foods.
Development of Student’s Worksheet using Project-Based Learning (PjBL) on Ecosystem Concept for Class X SMA Riana, Febi; Sunarto, Sunarto; Mamangkey, Jendri
Bioeduca : Journal of Biology Education Vol 6, No 1 (2024): Bioeduca : Journal of Biology Education
Publisher : Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/bioeduca.v6i1.17275

Abstract

The students' learning interest is decreasing due to the lack of interactive, enjoyable, and supportive instructional materials to help them achieve good learning outcomes. Therefore, researchers innovated by developing Learner Worksheets (LKPD) based on Project-Based Learning (PjBL). The research aims to assess the feasibility of PjBL-based LKPD through evaluations from experts (in content, media, and language) and students' responses. The research type used is Research and Development (RD). The assessment results from content experts showed a score of 82.03% "Very Feasible," media experts rated it 86.5% "Very Feasible," and language experts rated it 83% "Very Feasible," while students' response was 53% "Fairly Positive." Thus, it can be concluded that PjBL-based LKPD is feasible and can be used in education, especially in the field of biology
IDENTIFIKASI TUMBUHAN ANGIOSPERMS DI SMA NEGERI 42 JAKARTA SEBAGAI KONTEN MEDIA KOMIK PEMBELAJARAN MATERI BIODIVERSITAS Napitupulu, Agnes Ester; Adinugraha, Fajar; Mamangkey, Jendri
Cocos Bio Vol 8 No 2 (2023): Cocos Bio
Publisher : UNIVERSITAS HAMZANWADI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/cob.v8i2.24272

Abstract

Pengembangan komik tentang keanekaragaman tumbuhan dilakukan sebagai upaya untuk mengatasi rendahnya pengetahuan biodiversitas pada siswa. Tujuan penelitian ini untuk mengidentifikasi keanekaragaman tumbuhan Angiosperms di SMA Negeri 42 Jakarta. Selanjutnya, untuk mendeskripsikan komik yang sudah dibuat termasuk validasi dan persepsi siswa terhadap komik. Metode penelitian adalah eksplorasi lapangan dan pengembangan. Identifikasi dilakukan terdapat sembilan species yang dipilih untuk dijadikan konten komik pembelajaran. Hasil identifikasi menunjukkan bahwa terdapat delapan species dari Clade Eudicots dan satu species dari Clade Monocots. Komik yang dikembangkan diunggah di ResearchGate yaitu https://bit.ly/komikagnesetal. Komik pembelajaran yang dibuat memiliki kelebihan karena dibuat berdasarkan hasil penelitian identifikasi biodiversitas sekitar sekolah. Komik ini menunjukkan bahwa berdasarkan teori Contextual Teaching and Learning. Komik tersebut sudah divalidasi oleh tiga validator dan dinyatakan layak setelah revisi. Selanjutnya, siswa memberikan respon positif terhadap komik pembelajaran. Hadirnya komik pembelajaran keanekaragaman tumbuhan Angiosperms diharapkan dapat menuntaskan permasalahan rendahnya pengetahuan tumbuhan.
Pengembangan Modul Keanekaragaman Hayati Berbasis Pangan Tradisional Nusa Tenggara Timur Evi Yulinda Tpoenifu; Jendri Mamangkey; Marina Silalahi
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8370

Abstract

This study aimed to develop a module for biology based on the local wisdom of East Nusa Tenggara (NTT) traditional cuisine for high school students, particularly for the 10th grade, focusing on the topic of biodiversity. This also focused on assessing the feasibility of the module and evaluating students' responses to its development.The development model used in this study was research and development (R&D), involving several stages of potentials and problems, data collection, product design, design validation, and design revision followed by collecting students’ responses to the module.The validation results from content experts, media experts, and language experts indicated that the local wisdom-based biology module was deemed valid. The average scores obtained from each expert's validation are as follows, content validation with a score of 0.81 in the "strongly valid" category as well as media validation with a score of 0.80 and language validation with a score of 0.79 in the "valid" category.The result related to students’ responseswas also highly positive with an average percentage of 88,8% in the "excellent" category. This study is expected to serve as a learning resource for students, helping them understand the biodiversity material that is correlated with local wisdom. It aims to provide students with values of local wisdom and contribute to the preservation of environmental traditions.
Potential Probiotic Yeasts of the Pichia Genus Isolated from ‘Dadih’, a Traditional Fermented Food of West Sumatra, Indonesia Chihombori, Tatenda Calvin; Mustopa, Apon Zaenal; Astuti, Rika Indri; Mutiara, Ilma; Refli, Redoyan; Umami, Rifqiyah Nur; Fatimah; Irawan, Herman; Ekawati, Nurlaili; Trinugroho, Joko P; Akmaliyah, Rizna; Chairunnisa, Sheila; Amani, Febriyanti Nur; Manguntungi, Baso; Hertati, Ai; Mamangkey, Jendri
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.320-340

Abstract

Fermented buffalo milk, known as dadih, serves as a reservoir of potential probiotic yeasts. Over the past two decades, probiotic yeasts have gained increasing attention in both basic and clinical sciences due to their health benefits. This study aimed to isolate and characterize probiotic yeasts from dadih. Yeasts were isolated using yeast Extract, peptone, and dextrose (YPD) medium, and molecularly identified through 18S-rRNA sequencing. Probiotic potential was assessed by evaluating resistance to acidic pH, bile salts, proteolytic, lipolytic, and hemolytic activities. Secondary metabolites produced during fermentation were tested for antimicrobial properties. GBT30 and GBT37 isolates were selected based on their superior performance in probiotic property assays for further analysis. Molecular identification revealed these isolates as Pichia occidentalis (GBT30) and Pichia kudriavzevii (GBT37). Both strains demonstrated in vitro survivability under simulated gastrointestinal conditions and exhibited antimicrobial activity. Whole-genome sequencing of P. kudriavzevii GBT37 identified a genome size of 10,906,850 base pairs, distributed across four chromosomes with a GC content of 38.26%. Notably, secondary metabolite biosynthesis genes were located on contig 7. In addition, 26 probiotic-related genes, including GSY1, HSC82, HSP104, TPS1, ARN1, FLO1, ALA1, SIR2, and others, were identified in P. kudriavzevii GBT37, indicating its potential as a probiotic yeast. The traditional fermentation process of dadih offers probiotic yeasts with promising health benefits, supporting its potential as a functional food.
Peningkatan Keterampilan Berbasis Produk Lokal Terfermentasi Melalui Pembuatan Tempe Koro Benguk bagi Siswa SMA Ratnapuri, Adisti; Mamangkey, Jendri; Sunarto, Sunarto; Silalahi, Marina; Adinugraha, Fajar; Wahyuningtyas, Riska Septia; Septiani, Aulia; Lomo, Jhoni Yakobus; Sembiring, Geskia Avikasari; Andika, Vrista; Angela, Ezra; Serafin, Anne
Reswara: Jurnal Pengabdian Kepada Masyarakat Vol 6, No 1 (2025)
Publisher : Universitas Dharmawangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46576/rjpkm.v6i1.5195

Abstract

Pendekatan pengetahuan lokal pada produk terfermentasi (etnomikrobiologi) bertujuan menggabungkan kaijan produk lokal terfermentasi dengan hasil refleksi pengetahuan lokal. Etnomikrobiologi dapat menunjang keterampilan para siswa untuk mengolah produk lokal yang melibatkan aktivitas, salah satunya tempe koro benguk (Mucuna pruriens) yang memiliki nilai nutrisi tidak kalah dari tempe pada umumnya. Oleh karena itu, urgensi edukasi dan sosialisasi proses pembuatan perlu dilaksanakan untuk menunjang pengetahuan produk-produk bagi para siswa, serta dapat meningkatkan keterampilan mereka setelah memahami teori produk lokal terfermentasi. Tujuan kegiatan pengabdian ini yaitu mentransfer pengetahuan melalui sosialisasi teori dan praktik pembuatan tempe koro benguk kepada para siswa dan Guru di SMA Yadika 8 Jatimulya. Pelatihan ini dilaksanakan di SMA Yadika 8 Jatimulya, Kota Bekasi, Jawa Barat. Kegiatan terlaksana pada bulan Juli 2024. Pelaksanaan pengabdian kepada masyarakat (PKM) dilaksanakan dengan dua bagian utama. Bagian pertama yaitu sosialisasi dalam bentuk pemaparan materi kegiatan PKM. Bagian kedua yaitu pelatihan langsung pembuatan tempe berbahan dasar kacang koro benguk. Kegiatan PKM yang dilaksanakan di SMA Yadika 8 Jatimulya pada para siswa dapat memberikan pengetahuan dan pemahaman tentang integrasi kearifan lokal kacang koro benguk dan pemanfaatannya sebagai produk pangan tempe koro benguk. Hasil evaluasinya 64% siswa menyatakan kegiatan PKM sangat bermanfaat, sebanyak 70% siswa tertarik untuk membuat tempe koro benguk dan sebanyak 76% siswa berpendapat bahwa kegiatan PKM pembuatan tempe koro benguk sesuai dengan kebutuhan siswa dalam menunjang pembelajaran materi bioteknologi disekolah. Praktik pembuatan tempe koro benguk menjadi bagian penting pada pengembangan pengetahuan dan keterampilan siswa yang terintegrasi langsung dengan kearifan lokal serta memberikan pemahaman siswa pentingnya menjaga lingkungan untuk keberlangsungan hidup keanekaragaman hayati sebagai penunjang pembelajaran siswa
Medium Optimization for Recombinant Human Papillomavirus Type 52 L1 Protein Production in Pichia pastoris GS115 Platform on Bioreactor Scale Mustopa, Apon Zaenal; Nur Amani, Febriyanti; Irawan, Herman; Novianti, Ela; Swasthikawati, Sri; Ekawati, Nurlaili; Nurfatwa, Maritsa; Joko Wahyono, Daniel; Juanssilfero, Ario Betha; Mamangkey, Jendri; Purnomo, Yudi; Hertati, Ai; Wijaya, Hans; Dewi, Kartika Sari; Ningrum, Ratih Asmana
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1283-1294

Abstract

Human papillomavirus (HPV) stands as the primary etiological agent in the development of invasive cervical cancer worldwide. The L1 protein is a pivotal constituent of prophylactic HPV vaccines. Notably, HPV type 52 is one of the most prevalent genotypes found in squamous cell carcinoma cases in Indonesia. This research endeavor aims to enhance the productivity of recombinant HPV-52 L1 protein by optimizing the culture conditions of P. pastoris GS115 cells. In this study, we conducted trials employing 17 different media variants to optimize the expression of recombinant HPV-52 L1 protein. The results from small-scale experiments revealed three media, namely SYN6.10, BMMY, and SYN6.1, which exhibited promising yields of recombinant HPV-52 L1 protein as assessed through ELISA or immunoassay analysis. We succeeded in refining the SYN6.10 derivative, denoted as SYN6.10b, specifically designed for use in 1-L and 5-L bioreactors. This achievement was realized by adjusting Trace Element Solution (TES) and Vitamin Solution (VS) concentrations and implementing a methanol fed-batch phase with the addition of 0.3% methanol after 24 and 48 hours of fermentation in the P. pastoris medium. Further visualizations through SDS-PAGE and western blot analysis confirmed the protein after 72 hours of fermentation in a 1-L bioreactor using the SYN6.10b medium. In conclusion, the SYN6.10b medium required a 72 hours fermentation period to successfully express recombinant HPV-52 L1 protein in the P. pastoris platform.
Co-Authors Abdillah, Latifah Ningrum Adinugraha, Fajar Adisti Ratnapuri Adrian Hartanto, Adrian Ai Hertati, Ai Akmaliyah, Rizna Amani, Febriyanti Nur Andika, Vrista Andry Pratama, Andry Angela, Ezra Anggi Izdihar Mahaswari Apon Zaenal Mustopa Arfa Yanti, Nur Ario Betha Juanssilfero Aritonang, Netty Arman Harahap Aryanti Pada Soa, Christina Asri Razad Aulia Septiani Baehaqi Bethaniel Roy Matthew Cengiz Kayacilar Chairunnisa, Sheila Chihombori, Tatenda Calvin Dana Briggs Dian Winarty Budadana, Christyani DWI SURYANTO Ekawati, Nurlaili Elida Octavia Evi Yulinda Tpoenifu Fadjri, Corrina Lailatul Fatimah Frans Grovy Naibaho Gulo, Joice Elma Miranda Gusniarty Simanjuntak Gusti Ayu Kade Sutariati Hibatullah, Alnadia Yusriya Huda Shalahudin Darusman Huda, Muhammad Komarul Hutabarat, Anne Serafin I wayan Teguh Wibawan Irawan, Herman Ivan Julius Rajagukguk Jajang Gumilar Janed Lauren Soplantila Janed Lauren Soplantila Jefrino Reis Pereira Joice Elma Miranda Gulo Joko Pamungkas Joko Wahyono, Daniel Kartika Sari Dewi KIKI NURTJAHJA Lilis Suryaningsih Lomo, Jhoni Yakobus Lucas William Mendes Lukas Pardosi Mada Triandala Sibero Manguntungi, Baso Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Medyawanti Pane Mustopa, Apon Mutiara, Ilma Muttaqin, Salwa Zainum Napitupulu, Agnes Ester Napitupulu, Alexis Rachel Maria Rosauli Nelius Harefa Nitte, Tri Maharani Permata Nive Winda Sari Lumban Tungkup Novianti, Ela Nowlinda Kamuihkar Nur Amani, Febriyanti Nurfatwa, Maritsa Octavia, Elida Ode Adi Parman Rudia, La Ode Muhamad Iman Sulaiman, La Pereira, Jefrino Reis Pilidai Endorina Nadapdap Purba, Dian Pirma Natasari Putranto, Wendry Raden Mohamad Herdian Bhakti RATIH ASMANA NINGRUM Ratnapuri, Adisti Refli, Redoyan Ria Simanjuntak, Desy Riana, Febi Rifqiyah Nur Umami, Rifqiyah Nur Rika Indri Astuti Ripaldo Sihombing Riska Septia Wahyunigtyas Riska Septia Wahyuningtyas Rivaldi Sitinjak Rosdiana Virginia Rosenta Purba, Ika Sakka, Deni Frans Salwa Zainum Muttaqin Santhya Anaomi Tamansa Sari, Isti Kartika Sembiring, Geskia Avikasari Septiani, Aulia Serafin, Anne Setiyadi Putranto, Wendry Siahaan, Frisca Olivia Sitinjak, Rivaldi Situmorang, Agnes Feronika Sri Swasthikawati, Sri SUNARTO Sunarto Sunarto Sunarto Sunarto Talitha Amalia Tesalonika Febriani Zebua Tpoenifu, Evi Yulinda Tresjia C Rakian Tri Maharani Permata Nitte Trinugroho, Joko P Wafaretta, Erona Wahyuningtyas, Riska Septia Wibowo, Des Saputro Wijaya, Hans Yudi Purnomo Zaenal, Apon Zefanya Sipahelut