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SEDIMENTASI DAN SEBARAN MAKROZOOBENTOS DI KAWASAN LAGUNA SEGARA ANAKAN NUSAKAMBANGAN, CILACAP Okliandi Saputra; Yudi Nurul Ihsan; Lintang Permata Sari Yuliandi; Yeni Mulyani
Jurnal Perikanan Kelautan Vol 8, No 1 (2017): Jurnal Perikanan dan Kelautan Unpad
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran

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Abstract

Penelitian ini dilaksanakan pada bulan Februari 2016 di Laguna Segara Anakan dengan tujuan untuk mengetahui laju sedimentasi dan sebaran makrozoobentos di Laguna Segara Anakan. Metode penelitian yang digunakan adalah metode survey Pengambilan Sampel makrozoobentos dan sedimen pada setiap stasiun dilakukan dengan menggunakan Grab Sampler dan sampel sedimen terakumulasi diambil menggunakan Sedimen trap. Metode analisis data yang digunakan adalah metode deskriptif. Laju sedimentasi di Laguna Segara Anakan sangat tinggi. Laju sedimentasi yang paling tinggi terdapat di Stasiun 2 (muara sungai Citanduy) yaitu 1,88 ml/m3/hari. Hal ini disebabkan karena pada stasiun ini terjadi perubahan kecepatan arus yang melambat cukup tinggi sehingga banyak sedimen terakumulasi di daerah ini. Kondisi lingkungan Laguna Segara Anakan masih baik untuk kelangsungan hidup makrozoobentos, kecuali pada stasiun 4 (daerah yang berhubungan langsung dengan laut). Namun, dilihat dari nilai indeks keanekaragaman beberapa stasiun yang mendekati kriteria keanekaragaman rendah (H’<1) dan indeks dominansi yang mendekati kriteria ada jenis yang dominansi (C<1) menunjukkan bahwa perairan Segara Anakan sudah mulai mengalami degradasi lingkungan.
Aktivitas Antioksidan Dan Antibakteri Ekstrak Jeroan Teripang Holothuria atra Dari Perairan Pulau Biawak Kabupaten Indramayu Dewi Oktaviani; Yeni Mulyani; Emma Rochima
Jurnal Perikanan Kelautan Vol 6, No 2(1) (2015): Jurnal Perikanan dan Kelautan
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran

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Abstract

Penelitian ini dilakukan untuk mengetahui kandungan metabolit sekunder pada jeroan teripang Holothuria atra dan potensinya sebagai antioksidan dan antibakteri. Penelitian ini dilaksanakan pada bulan November 2014 s/d Mei 2015 di Laboratorium Bioteknologi Kelautan Fakultas Perikanan dan Ilmu Kelautan, Universitas Padjadjaran. Penelitian ini menggunakan metode eksperimental di laboratorium yang terbagi menjadi dua tahap. Tahap pertama ekstraksi dengan metode maserasi dan identifikasi metabolit sekunder dilanjutkan tahap kedua uji antioksidan menggunakan metode DPPH dan uji antibakteri menggunakan metode difusi agar. Kesimpulan dari penelitian ini bahwa ekstrak jeroan teripang H.atra memiliki nilai rendemen 4,177% dan mengandung senyawa alkaloid, triterpenoid dan saponin. Nilai IC50 ekstrak jeroan teripang H.atra 126,19 ppm termasuk antioksidan kategori sedang dan diameter zona hambat ekstrak jeroan teripang H.atra 7,79 mm pada konsentrasi 10.000 ppm sehingga termasuk antibakteri kategori sedang.Kata Kunci: Antioksidan, Antibakteri, Holothuria atra, Metabolit Sekunder 
EFEKTIVITAS JAMUR Marasmius sp UNTUK MENDEGRADASI LIMBAH PENGOLAHAN AGAR (Gracilaria sp) SEBAGAI BAHAN BAKU BIOETANOL Rudy Kurniadi; Zahidah Hasan; Yeni Mulyani
Jurnal Perikanan Kelautan Vol 3, No 4 (2012)
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran

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Abstract

Penelitian ini bertujuan mengetahui efektifitas dosis inokulum dan lama waktu fermentasi jamur Marasmius sp untuk mendegradasi limbah industri pengolahan agar Gracillaria sp sebagai bahan baku pembuatan bioetanol. Metode yang digunakan dalam penelitian ini adalah metode eksperimen. Percobaan menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 3x3 faktor pertama adalah dosis inokulum yaitu D1=5%, D2=7,5%, D3=10%, sedangkan faktor yang kedua adalah lamanya fermentasi yaitu W1= 2 minggu, W2= 3 minggu, W3= 4 minggu. Data hasil penelitian berupa kadar lignoselulosa limbah agar, jumlah glukosa (mg/mL) yang dihasilkan dalam proses hidrolisis, dan jumlah bioetanol (%). Hasil pengujian memperlihatkan bahwa dosis 10 % dengan lama waktu fermentasi 3 minggu merupakan kombinasi yang terbaik untuk menghasilkan kadar glukosa tertinggi yaitu sebesar 0,072 mg/mL
Penentuan Tipe Pantai di Kawasan Pantai Wisata Batu Karas, Kabupaten Pangandaran, Jawa Barat Annisa Putri Fitrian; Ankiq Taofiqurohman; Yeni Mulyani; Wahyuniar Pamungkas
Buletin Oseanografi Marina Vol 10, No 3 (2021): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v10i3.31990

Abstract

Pantai merupakan salah satu objek wisata alam yang paling diminati pengunjung, namun memiliki risiko bahaya tersendiri yang patut diwaspadai. Risiko bahaya yang melekat dimiliki oleh setiap pantai adalah bahaya akibat karakteristik fisik pantai. Pantai Wisata Batu Karas merupakan salah satu objek wisata pantai terfavorit di Jawa Barat dengan peningkatan jumlah pengunjung setiap tahunnya. Selain memiliki berbagai daya tarik wisata, Pantai Batu Karas memiliki risiko bahaya fisik. Risiko bahaya fisik yang dominan di pesisir selatan Jawa Barat, termasuk Pantai Wisata Batu Karas, adalah gelombang pecah karena berbatasan langsung dengan Samudera Hindia yang memicu risiko gelombang tinggi. Keselamatan wisata merupakan hal yang harus diutamakan untuk mencegah kerugian yang mungkin terjadi akibat risiko bahaya. Hal ini dapat dilakukan dengan melakukan penilaian bahaya fisik pantai untuk selanjutnya dapat dijadikan bahan pertimbangan dalam pengelolaan wisata pantai. Riset ini bertujuan untuk menentukan tipe pantai Batu Karas sebagai langkah awal penilaian bahaya fisik pantai akibat gelombang pecah di Pantai Wisata Batu Karas. Riset ini dilakukan pada bulan Januari – Mei 2020, dengan area yang diteliti adalah sepanjang Pantai Wisata Batu Karas. Metode yang digunakan dalam riset ini yaitu metode kuantitatif, selanjutnya dilakukan penentuan tipe pantai yang mengacu pada metode yang dikembangkan oleh Short (1996). Parameter yang digunakan dalam riset ini adalah tinggi dan periode gelombang pecah, kecepatan jatuh sedimen, dan nilai tunggang pasut. Hasil yang didapatkan dari riset ini adalah Pantai Batu Karas memiliki tipe pantai longshore bar and trough, yang selanjutnya dapat digunakan untuk penilaian tingkat bahaya fisik di Pantai Wisata Batu Karas. Beach is one of the most popular natural attractions for visitors, but has its own dangers that need to be watched out for. The inherent hazard risk possessed by each beach is the danger due to the physical characteristics of the beach. Batu Karas Tourism Beach is one of the most favorite beach attractions in West Java with an increasing number of visitors every year. Apart from having various tourist attractions, Batu Karas Tourism Beach also has a risk of physical danger. The dominant physical hazard risk on the southern coast of West Java, including Batu Karas Tourism Beach, is the breaking waves because it is directly adjacent to the Indian Ocean which triggers a high risk of waves. Tourism safety is a matter that must be prioritized to prevent losses that may occur due to hazard risks. This can be done by assessing the physical hazard of the beach which can then be used as a consideration in the management of beach tourism. This research aims to determine the type of beach as an initial step to assess the physical beach hazards in Batu Karas Beach, Pangandaran Regency, West Java. This research was conducted from January – May 2020 and focused on the Batu Karas shoreline area. Quantitative method was used to determine the beach type by referring to the method developed by Short (1996). The parameters used in this research are height and period of the breaker wave, sediment fall velocity, and tide range. The result shows that Batu Karas Beach has longshore bar and trough type, which can then be used for assessing the level of physical hazards at Batu Karas Tourism Beach.
Zooplankton sebagai Bioindikator Kesuburan Perairan di Hutan Mangrove Teluk Ciletuh, Kabupaten Sukabumi Indah Riyantini; Mochamad Rudyansyah Ismail; Yeni Mulyani; Gustiani -
Akuatika Indonesia Vol 5, No 2 (2020): Jurnal Akuatika Indonesia (JAkI)
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan Universitas Padjadjaran, Grha. Kandaga (P

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jaki.v5i2.29021

Abstract

Kawasan mangrove di Teluk Ciletuh dijadikan sebagai kawasan ekowisata mangrove. Kegiatan wisata ini menyebabkan terjadinya perubahan lingkungan di kawasan mangrove. Zooplankton dapat digunakan sebagai pengukur tingkat produktivitas suatu perairan. Tujuan dari penelitian ini untuk mengidentifikasi dan menghitung keanekaragaman serta kelimpahan zooplankton di Hutan mangrove perairan Teluk Ciletuh, sebagai bioindikator kesuburan perairan. Metode pengambilan data dilakukan secara purposive sampling. Pengambilan data dilakukan pada 3 stasiun penelitian berdasarkan komposisi mangrove yang berbeda dengan dua kali pengulangan. Terdapat 41 genus zooplankton yang didominansi oleh genus Cyclops sp. dengan nilai keanekaragaman sebesar 0,47 - 1,42. Kualitas air di perairan mangrove Teluk Ciletuh berada pada kondisi yang kurang produktif karena memiliki nilai pH, salinitas, dan DO dibawah baku mutu perairan. Perairan tersebut memiliki status mesotrofik, dengan kelimpahan 7,39 – 72,06 ind/L. Berdasarkan kelimpahan zooplankton dan kualitas perairan, maka kesuburan perairan di Hutan Mangrove Teluk Ciletuh termasuk kategori cukup subur atau tingkat kesuburan sedang.
In Vivo Test of Rhizophora mucronata Mangrove Extract From Pangandaran Coast Towards Nile Tilapia Oreochromis niloticus infected by Vibrio harveyi Yeni Mulyani; Kiki Haetami; Lesta Krismawati Baeha; Sulastri Arsad; Fiddy Semba Prasetiya
Journal of Aquaculture and Fish Health Vol. 9 No. 2 (2020): JAFH Vol. 9 No. 2 June 2020
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v9i2.16211

Abstract

This study aims to determine the effectiveness of Rhizophora Mucronata’s bark extract in curing Vibrio harveyi infection in nile tilapia fish by challenge testing (in vivo test). It was conducted in October 2018-May 2019 at the Central Laboratory of Universitas Padjadjaran and Building-4 of Aquaculture and Hatchery Laboratory in the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. This research used microdilution method to determine the Minimum Inhibitory Concentration (MIC) value of R.mucronata’s bark extracts and laboratory experimental method with a Completely Randomized Design (CRD) model for challenge testing (in vivo) which consisted of 5 treatments and 3 replicates where the given treatment varied in concentrations. These various concentrations that were given started from A (0 ppm / control), B (16 ppm), C (32 ppm), D (48 ppm), and E (64 ppm). The observed parameters were inhibitory antibacterial activity for MIC and survival rate, as well as clinical symptoms and water quality for the challenge test. Survival Rate data of nile tilapia seedlings was analyzed by regression analysis. The observation results of MIC, MBC values, clinical symptoms and water quality were analyzed descriptively. The results showed the Minimum Inhibitory Concentration value of R.mucronata bark extract at a concentration of 6,250 ppm while its Minimum Bacteriocidal Concentration value at 50,000 ppm. The fish tolerance test results towards the extract (LC50 test) is 64 ppm. Differences in treatment produced results that have significant effects on survival rate. The use of Rhizophora Mucronata bark extract at a dose of 64 ppm resulted in the highest survival rate of Nile tilapia fish as high as 76.66%.
Study of Phytochemistry and Potential of Endophyte Fungi Extract in Avicennia marina Roots as Antioxidants Inhibiting Early Aging Muhammad Andhika Pratama; Mochammad Agung Seno Pambudi; Eri Bachtiar; Mochamad Rudyansyah Ismail; Yeni Mulyani; Sulastri Arsad; Fiddy Semba Prasetiya
Journal of Aquaculture and Fish Health Vol. 11 No. 1 (2022): JAFH Vol. 11 No. 1 February 2022
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v11i1.24502

Abstract

The mangrove plants has long been utilized as traditional medicinal ingredients for treatments of various diseases in favour of bioactive components from its secondary metabolites. Secondary metabolites from mangroves could act as an antioxidant to prevent oxidative stress. Mangrove may contain endophyte fungi in its tissues that are capable of producing secondary metabolites. In theory, endophyte fungi isolated from a plant can produce secondary metabolites similar to those of the original plants or even in relatively high numbers. In this research, mangrove species Avicennia marina was sampled from Blanakan, Subang District, West Java. Endophyte fungi were isolated from the mangrove roots that had been obtained. The experimental method was used to test antioxidant activitiy using DPPH (1,1-diphenyl-2-picrylhidrazyl) method and phytochemical content, with three replicates for each tests. Fungal isolates were coded according to the distance of the roots from the main mangrove tree, namely STAD and STAJ that represents “close” and “far” roots. Our results showed that the enviromental parameters, namely salinity, dissolved oxygen, and temperature meet the quality standards and mangrove life in the tropics. The A. marina type of substrate muddy soil and has a pH value, which did not meet the quality standards for mangrove waters. As for antioxidant activity, both extracts could reduce DPPH free radicals. Further determination of the IC50 value of the two extracts showed that the endophytic fungi STAD and STAJ had IC50 values of 97.8 ppm (classified as strong) and 822.56 ppm (classified as very weak) in the DPPH radical immersion method. Phytochemical test revealed that the two extracts of fungal isolates contain bioactive compounds, where flavonoids and alkaloids were identified in isolate STAD, whereas STAJ was positive for tannins and alkaloids content.
Microplastic tracking from Pacific garbage to Northern Indonesia Sea Agung Ramos; Noir P Purba; Ibnu Faizal; Yeni Mulyani; Mega L. Syamsuddin
Jurnal Perspektif Pembiayaan dan Pembangunan Daerah Vol. 6 No. 1 (2018): Jurnal Perspektif Pembiayaan dan Pembangunan Daerah
Publisher : Program Magister Ilmu Ekonomi Pascasarjana Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (906.91 KB) | DOI: 10.22437/ppd.v6i1.5178

Abstract

This study aims to examine the pattern of microplastic waste movement of Pacific Garbage Patch (PGP) that has the potential to enter the north of Indonesia sea. This study very important because microplastic affected the regional tourism activities, water pollution, and economics of fisherman and local society. The impact is very widerange to local and national economics. The method used is a simulation and descriptive analysis. The simulation method used is hydrodynamic simulation and trajectory of particles conducted for 365 days in 2016. The study areas in this study include eastern Indonesia, the Philippines, and the Pacific Ocean. The data used are wind, tidal, bathymetry, microplastic weight, and microplastic flux. The simulation results show the movement of microplastic at point source 1 tends to the northeast with a clockwise circular motion. The simulation results at source point 2 indicate that the microplastic movement tends to revolve around the source point in a clockwise circular motion. The result of simulation of source point 3 shows that the microplastic movement tends to the north with the circular motion and left-right movement. The displacement of microplastic particles within a year is only 0.11 - 8.94 km from the source point. This study showed that within a year, microplastic waste from PGP does not have the potential to enter the waters of Indonesia.
Arohynapene A Produced by Penicillium steckii JB-NW-2-1 Isolated from Avicennia marina (Forssk.) Vierh and Its Cytotoxic Activities Yeni Mulyani; Wahyu Syafriansyah; Asri Peni Wulandari; Azmi Azhari; Sari Purbaya; Aprilia Permata Sari; Galih Bayu Pratama; Fajar Fauzi Abdullah; Kindi Farabi; Unang Supratman; Yoshihito Shiono
Indonesian Journal of Chemistry Vol 24, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.83982

Abstract

Mangrove-associated endophytic fungi are producers of secondary metabolites in unique and diverse structures with interesting biological activities such as antiviral, antifungal, antibacterial, anti-inflammatory, and cytotoxic agents. Endophytes play an important role in the physiological activities of the host plants, influencing the improvement of resistance to stress, insects, nematodes, and diseases. In this study, arohynapene A, a polyketide compound, was successfully isolated from the mangrove-derived fungus Penicillium steckii JB-NW-2-1 obtained from mangrove plant Avicennia marina (Forssk) Vierh. The structure was determined by a spectroscopic method including IR, MS, 1D-, and 2D-NMR techniques. This compound was evaluated for cytotoxic activities using resazurin assay against four cancer cells, HeLa cervical, MCF-7 breast cancer, B16-F10 melanoma, and A549 lung adenocarcinoma. The results showed no significant activities against all cancer cells tested (IC50 > 500 µM).
Diketopiperazine Cyclo-(S-Pro-R-Leu) Produced by Periconia pseudobyssoides K5 Isolated from Toona sureni (Meliaceae) and its Heme Polymerization Inhibition Activity Azhari, Azmi; Harneti, Desi; Wulandari, Asri Peni; Mulyani, Yeni; Purbaya, Sari; Sari, Aprilia Permata; Pratama, Galih Bayu; Supratman, Unang; Shiono, Yoshihito
Makara Journal of Science Vol. 27, No. 1
Publisher : UI Scholars Hub

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Abstract

Fungi endophytes are living microorganisms colonizing inside the internal tissue of a plant and provide ecological benefits for their host. Endophytes provide various metabolites for plant adaptation toward biotic and abiotic stresses and have tremendous pharmacological activities. Toona sureni (T. sureni) (Meliaceae) belongs to the Toona genera and is reported to have antimalarial activity. To determine the compounds produced by the endophytic fungus from this plant, we isolated a compound from Periconia pseudobyssoides (P. pseudobyssoides) K5 endophytes from the stem bark of T. sureni. Diketopiperazine cyclo-(S-Pro-R-Leu), a non-ribosomal peptide, was isolated from brown rice fermented at 28 ± 2 ℃ for 30 days. The structure was determined by spectroscopic methods including fourier-transform infrared spectroscopy, mass spectrometry, and 1D and 2D nuclear magnetic resonance techniques. This compound was evaluated for heme polymerization inhibition activity (HPIA) with an IC50 value of 9.89 ± 0.24 mmol/L compared with positive control chloroquine phosphate with an IC50 value of 3.08 ± 0.58 mmol/L. This compound has been categorized as having low activity three times lower than positive control chloroquine phosphate. This information provides new leads about the compound diketopiperazine cyclo-(S-Pro-R-Leu) produced by P. pseudobyssoides K5 endophytes having low activity in inhibiting heme polymerization. In the future, to explore the potency of this compound as antimalarial agent, the other antimalarial test such as lactate dehydrogenase assay might be useful.
Co-Authors Agung Ramos Agung, M. Untung Kurnia Aisyah Aisyah alfiah alfiah Ani Mardiastuti Ankiq Taofiqurohman Annisa Putri Fitrian Aprilia Permata Sari Ardhiansyah, Rasendriya Naufal Arief, Mochamad Candra Wirawan Asri Peni Wulandari Asri Peni Wulandari, Asri Peni Azmi Azhari Azmi Azhari, Azmi Bai'un, Nurul Hanifah Chandra, Vinoba Dede Supriatna Desi Harneti Putri Huspa Dewi Oktaviani Dhian Ririn Lestari, Dhian Ririn Donny Juliandri Prihadi Emma Rochima Eri Bachtiar Evi Liviawaty Fahrian, Hijrah Fajar Fauzi Abdullah Fiddy Semba Prasetiya Fiddy Semba Prasetiya Galih Bayu Pratama Gustiani - Husnul Khotimah Ibnu Faizal Iis Rostini Iklima Hasanah Indah Riyantini Indah Riyantini Indah Riyantini Jannah, Rodiatul Julaehah, Julaehah Kalysta Fellatami Karimah Syakirotin Kiki Haetami Kindi Farabi Lesta Krismawati Baeha Lestari, Indri Liawati, Liawati Lintang Permata Sari Yuliandi Madaniah AS, Ida Mahdalena Mahdalena Mala Septiani Manshur Awalludin Mega L. Syamsuddin Mochamad Rudyansyah Ismail Mochamad Untung Kurnia Agung Mochamad Untung Kurnia Agung Mochammad Agung Seno Pambudi Muhammad Andhika Pratama Muhammad Untung Kurnia Agung Muhammad Untung Kurnia Agung Mutaqin, Moh Fikri Tanzil Noir P Purba Noir Primadona Purba Norsyehan Nuraini, Eviatun Nurfitriani, Evi Okliandi Saputra Pamungkas, Wahyuniar Pramunandya, Raka Aditya Pratama, Galih Bayu Purbaya, Sari Rega Permana Rizany, Ichsan Rohyati, Rohyati Rudy Kurniadi Saptari, Muhammad Khairan Sari Purbaya Sari, Aprilia Permata Satrio Hadisnugroho Shiono, Yoshihito Solihat, Anisa Sulastri Arsad Sulehah, Sulehah sumarni, nini sunarto sunarto Syawaludin Alisyahbana Harahap Tri Dewi K Pribadi Unang Supratman Vega K. Nurulita Wahyu Syafriansyah Wahyuniar Pamungkas Widyasthana, Ferdiansyah Naufal Yoshihito Shiono Yudi Nurul Ihsan Yuniar Mulyani Zahidah - Zallesa, Sheila Zendina, Luthfia