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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Indonesian Journal of Human Nutrition Majalah Kesehatan FKUB Jurnal Respirasi (JR) Jurnal Ners CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL CRJIM (Clinical and Research Journal in Internal Medicine) Narra J Caring : Jurnal Pengabdian Masyarakat Cermin Dunia Kedokteran COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Asian Journal of Health Research Jurnal Kedokteran Komunitas (Journal of Community Medicine) The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Jurnal Penyakit Dalam Indonesia Journal of Law, Education and Business Indonesian Journal of Biomedicine and Clinical Sciences Deka in Medicine
Syifa Mustika
Brawijaya University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model: High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels Syifa Mustika; Nabila Ramadhani; Novita Apramadha Kartika Sari; Dian Handayani; Dewi Santosaningsih
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.08

Abstract

Dysbiosis, marked by a decline in Firmicutes (such as Clostridium spp.) and a rise in Proteobacteria (such as Escherichia coli), is recognized as a pathway linked to the progression of non-alcoholic fatty liver disease (NAFLD). High-fat food may influence the development of NAFLD. This study investigates the effect of different high-fat diets on gut microbiota composition and butyrate levels in a rat model. Four groups of Rattus norvegicus strain Wistar (n=27) were fed specific diets for 12 weeks: normal diet (ND, n=7), high-fat diet (HFD, n=6), western diet (WD, n=7), and high-fat-high-fructose diet (HFHFD, n=7). Stools were cultured on Eosin Methylene Blue (EMB) for Escherichia coli and Forget Fredette Agar (FFA) for Clostridium spp. Colonies were counted using Total Plate Count (TPC), and butyrate levels were measured with gas chromatography. Liver histology was assessed with the NAFLD activity score (NAS). Gut microbiota were analyzed using Crosstab and Chi-Square tests, and butyrate levels with the Kruskal-Wallis test. Butyrate levels decreased in NAFLD-inducing diet groups (HFD, WD, HFHFD) compared to ND (p=0.021). Escherichia coli colony counts were significantly higher in NAFLD-inducing diet groups (p=0.048), while Clostridium spp. counts were higher in ND (p=0.001). Liver histological changes, particularly in the WD group, resembled NAFLD. These findings imply that different high-fat diets can alter the gut microbiome, potentially influencing NAFLD development.
Effect of Multiple Types of High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels on NAFLD Rat Model: High-fat Diet Induction on Gut Microbiota Profile and Butyrate Levels Syifa Mustika; Nabila Ramadhani; Novita Apramadha Kartika Sari; Dian Handayani; Dewi Santosaningsih
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.08

Abstract

Dysbiosis, marked by a decline in Firmicutes (such as Clostridium spp.) and a rise in Proteobacteria (such as Escherichia coli), is recognized as a pathway linked to the progression of non-alcoholic fatty liver disease (NAFLD). High-fat food may influence the development of NAFLD. This study investigates the effect of different high-fat diets on gut microbiota composition and butyrate levels in a rat model. Four groups of Rattus norvegicus strain Wistar (n=27) were fed specific diets for 12 weeks: normal diet (ND, n=7), high-fat diet (HFD, n=6), western diet (WD, n=7), and high-fat-high-fructose diet (HFHFD, n=7). Stools were cultured on Eosin Methylene Blue (EMB) for Escherichia coli and Forget Fredette Agar (FFA) for Clostridium spp. Colonies were counted using Total Plate Count (TPC), and butyrate levels were measured with gas chromatography. Liver histology was assessed with the NAFLD activity score (NAS). Gut microbiota were analyzed using Crosstab and Chi-Square tests, and butyrate levels with the Kruskal-Wallis test. Butyrate levels decreased in NAFLD-inducing diet groups (HFD, WD, HFHFD) compared to ND (p=0.021). Escherichia coli colony counts were significantly higher in NAFLD-inducing diet groups (p=0.048), while Clostridium spp. counts were higher in ND (p=0.001). Liver histological changes, particularly in the WD group, resembled NAFLD. These findings imply that different high-fat diets can alter the gut microbiome, potentially influencing NAFLD development.
Co-Authors A.A. Ketut Agung Cahyawan W Achmad Rudijanto Adam Adam Ade Rahmawati Affa Kiysa Waafi Amalia, Dinda Amanda C Wowor Anton Komala Arifah, Nina Nur Bayu Eka Nugraha Bogi Pratomo Bogi Pratomo Bogi Pratomo Bogi Pratomo Bogi Pratomo Bogi Pratomo Brahmantyo, Herwindo Pudjo Budi Herlianto Budi Herlianto Budiarto, Andika Agus Caesar Ahmad Tidel Camoya Gersom Charisma Dian Simatupang Daryadijaya, Daya Daya Daryadijaya Dewi Santosaningsih Dewi Santosaningsih Dian Handayani Dian Handayani Dian Hasanah Diana Jeni H Diana Jeni H, Diana Jeni edy susanto Endang S. Rahayu Erlina B Fachrureza, Mochamad Fachrurrezza, Mochammad Faridah, Amirah Fathoni, Alfan Fiqih Faruz Gatya, Mifta Harijono Achmad Harijono Achmad Harijono Achmad Harijono Ahmad Hartaya, Arsy Hanandya Imroatussholihah, Al Indriyaningrum, Nurria Betty Indriyaningrum, Nurria Betty Jonny K. Fajar Kenty Wantri Anita, Kenty Wantri Komala, Anton Kongkam, Pradermchai Larasati, Putu Anissa Lia Sasmithae Lutfiyanto Nurhidayat M. Farid Rakhman Mariyatun Mariyatun, Mariyatun Marzuki, Mochammad Jalalul Michaela, Cleine Muli Yaman Muthiah, Mark Nabila Ramadhani Nabila, Salsala Sifa Nanik Triana Nasution, Muhammad Imanuddin Nina Nur Arifah Novita Apramadha Kartika Sari Nugraha, Bayu Eka Nugroho, Rama Tiar Nurlaili, Retti Nurmalita, Shafira Nurria Betty Indriyaningrum Pandu Tridana Sakti, Pandu Tridana Pramesi, Putrika C. Pratista Adi Krisna Pratomo , Bogi Prayitno, Tia Rahmi Priyanto, Tia Rahmi Putri, Dinda Amalia Eka Putri, Fildzah Karunia Rakhmadhan, Iraky Ramadi Satryo Wicaksono Ratnaningtyas, Yosefin RC Tarigan Renny Tandya Retti Nurlaili Reyhan , Muhammad Rosita, Firdha Ruliana Ruliana, Ruliana Rulli Rosandi Safira, Zahra Sagala, Ida Samichah Samichah Sasmithae, Lia Satiti, Ika Sembiring, Frinny Sholihah, Al Imroatus Sigit Triyus Priyantoro Sigit Triyus Priyantoro, Sigit Triyus Sopacua , Andre Supriono Supriono ., Supriono Supriono Supriono Supriono Supriono Supriono Supriono Susanti, Lia Susanto, Jefri P. Suyoso Suyoso Tarigan, RC Tiar Oktavian Effendi Triana, Nanik Vianty , Liku Wibowo, Bogi Pratomo Wijayanti Saputri, Nur Estu Yuni Rahmawati