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Kontaminasi Sumber Air oleh Cacing Usus dan Higiene Sanitasi sebagai Faktor Risiko Infeksi Helminthiasis pada Petani Yunita Armiyanti; Brillian Adexa Yudinda; Heni Fatmawati; Bagus Hermansyah; Wiwien Sugih Utami
Jurnal Kesehatan Lingkungan Indonesia Vol 22, No 1 (2023): Februari 2023
Publisher : Master Program of Environmental Health, Faculty of Public Health, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkli.22.1.60-68

Abstract

Latar belakang: Kabupaten Jember khususnya Kecamatan Jenggawah merupakan wilayah agraris dengan pekerjaan utama sebagai petani dan hampir separuh penduduknya tidak mempunyai fasilitas jamban untuk buang air besar. Kebiasaan defekasi yang tidak sehat masih banyak dilakukan oleh masyarakat petani, sehingga bisa mencemari sumber air dengan telur dan larva cacing usus yang berakibat terjadinya penularan helminthiasis. Penelitian ini bertujuan untuk mengetahui adanya kontaminasi sumber air (air sumur dan sungai) oleh cacing usus, faktor risiko higiene sanitasi dan hubungannya dengan prevalensi helminthiasis pada petani di Kecamatan Jenggawah, Kabupaten Jember.Metode: Penelitian ini dilakukan di Kecamatan Jenggawah yang sebagian besar masyarakatnya adalah petani. Jumlah petani yang bersedia terlibat dalam penelitian adalah 56, sehingga jumlah sampel feses dan air sumur yang diperoleh juga sebanyak 56. Sampel air sungai juga diambil untuk diperiksa. Sampel feses dan air diperiksa menggunakan metode sedimentasi dan flotasi untuk identifikasi telur dan larva cacing usus. Faktor-faktor risiko higiene dan sanitasi sebagai variabel bebas didapatkan dari kuesioner dan dianalisis dengan univariat, bivariat dan multivariat.Hasil: Karakteristik responden menunjukkan sebagian besar adalah laki-laki (53,51%), berusia 18-60 tahun (75%), berpendidikan rendah (53,57%), dan berpenghasilan rendah (71,43%). Sampel air sumur yang terkontaminasi telur dan larva cacing usus sebesar 22,2%, sedangkan sampel air sungai semuanya terkontaminasi. Prevalensi helminthiasis pada petani adalah 7,1%. Jenis cacing usus yang banyak ditemukan adalah Hookworm. Hasil uji bivariate (chi-square) menunjukkan kontaminasi sumber air oleh cacing usus berhubungan dengan prevalensi helminthiasis (ρ<0,05). Faktor risiko sanitasi yaitu jenis sumber air (air sungai atau air tanah) dan faktor risiko higiene kebiasaan mandi,cuci dan kakus (MCK) di sungai berhubungan dengan prevalensi helminthiasis (ρ<0,05).Simpulan: Sumber air yang terkontaminasi oleh telur dan larva cacing usus dan penggunaannya untuk kegiatan MCK dapat menjadi sumber transmisi infeksi helminthiasis pada masyarakat petani. ABSTRACTTitle: Contamination of Water Sources by Intestinal Worms and Sanitary Hygiene as Risk Factors for Helminths Infection in Farmers.Background: Jember Regency, especially Jenggawah Subdistrict, is an agricultural area with the main occupation as farmers and almost half of the population does not have latrine facilities for defecation. Unhealthy defecation habits are still widely practiced by farming communities, so that they can contaminate water sources with eggs and larvae of intestinal worms which result in helminthiasis transmission. This study aims to determine the contamination of water sources (well and river water) by intestinal worms, sanitation hygiene risk factors and their association with the prevalence of helminthiasis in farmers in Jenggawah District, Jember Regency.Method: This research was conducted in Jenggawah District where most of the people are farmers. The number of farmers who were willing to be involved in the study was 56, so the number of faecal and well water samples obtained was also 56. River water samples were also taken for examination. Stool and water samples were examined using sedimentation and flotation methods to identify eggs and larvae of intestinal worms. Hygiene and sanitation risk factors as independent variables were obtained from the questionnaire and analyzed using univariate, bivariate and multivariate analysis.Result: The characteristics of the respondents showed that most of them were male (53.51%), aged 18-60 years (75%), low educated (53.57%), and low income (71.43%). Well water samples were contaminated with eggs and larvae of intestinal worms by 22.2%, while river water samples were all contaminated. The prevalence of helminthiasis is 7.1%. The most common type of intestinal worm is Hookworm. The results of the chi-square test showed that contamination of water sources by intestinal worms was associated with the prevalence of helminthiasis (ρ<0.05). Sanitation risk factors, namely the kind of water sources (river water or ground water) and hygiene risk factors, i.e bathing, washing and latrine activities (MCK) habits in rivers are associated with the prevalence of helminthiasis (ρ<0.05).Conclusion: Water sources contaminated by eggs and larvae of intestinal worms and the habit of bathing, washing and latrine activities (MCK) in the river can be a source of transmission of helminthiasis infection in farming communities.
Hygiene and Sanitation Factors Affecting Contamination of Soil-Transmitted Helminths in Household Water Sources in Jember, Indonesia Epafroditus Sanjaya Adiguna; Wiwien Sugih Utami; Ika Rahmawati Sutejo; Bagus Hermansyah; Yunita Armiyanti
Althea Medical Journal Vol 9, No 3 (2022)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v9n3.2587

Abstract

Background: Water sources used by households are at risk of contamination by soil-transmitted helminths (STH). Hygiene and sanitation have an essential role in the transmission and contamination of STH. The objective of this study was to analyze whether there was an association between hygiene and sanitation factors with STH contamination in household water sources in Jember Regency, East Java Province, Indonesia.Methods: This cross-sectional analytic study was carried out from December 2020 to March 2021, including 46 samples from 23 respondents in Sukowono District and 23 respondents in Sukorambi District, Jember Regency, selected using a purposive sampling technique. Primary data on hygiene and sanitation risk factors were obtained from questionnaire interviews. The STH contamination in household water sources was examined by sedimentation and flotation methods. Data were analyzed using Fisher Exact test.Results: The identification of STH contamination in household water sources using microscope examination found hookworm species contaminated 8.7% (n=4) of water sources. There was a significant association between the type of human sewage disposal with STH contamination in household water sources (p=0.037). There was no association between hygiene and other sanitation factors with STH contamination in household water sources (p>0.05).Conclusions: Human sewage disposal that goes directly to sewers and rivers can cause contamination of water sources by STH. Therefore, it is important to dispose of human waste in septic tanks that comply with health requirements to prevent the transmission of STH to humans through water sources.
Increased of IL-4 and Ig E Level in Infection Hookworm in Plantation Workers in Jember Bagus Hermansyah; Yunita Armiyanti; Wiwien Sugih Utami
Journal of Agromedicine and Medical Sciences Vol 9 No 1 (2023)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v9i1.37301

Abstract

Hookworm infection is a disease that is included in the neglected tropical disease. Hookworm infection is suffered by many plantation workers who are closely related to the soil in daily life. Immune response in hookworm-infected individuals will experience disorders, especially in eosinophilia, mastocytosis, and IgE stimulation. It is therefore important to know the immune response to hookworm infections in plantation workers. In this study, eosinophilia was calculated on peripheral blood smears and counts of IL-4 and Ig-E levels by ELISA. The results showed an increase in eosinophil levels from blood samples of patients and a significant increase in IL-4 and IgE levels based on the results of statistical analysis. Keywords: hookworm, plantation worker, IL-4, and IgE
Knowledge and Behavior as Risk Factors for Intestinal Worm Contamination on Raw Vegetables Food Traders in Pakusari District Jember Regency Yoga Setyo; Wiwien Sugih Utami; Heni Fatmawati; Yunita Armiyanti; Bagus Hermansyah
Jurnal Ilmiah Kesehatan Vol 16 No 01 (2023): Jurnal Ilmiah Kesehatan (Journal of Health Science) 
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/jhs.v16i01.3192

Abstract

Intestinal parasites are one of the leading public health problems worldwide, with an incidence of 24% worldwide and 45-65% in Indonesia. Intestinal parasites, especially intestinal worms, or soil-transmitted helminths (STH), significantly contribute to gastrointestinal diseases worldwide. Intestinal worm infections occur in rural and urban populations, especially in people who have the habit of eating with dirty hands, using unhygienic toilets, and consuming contaminated food, water, or soil. Fresh vegetables are a means that support parasitic intestinal infections, especially for traders who must be able to prevent intestinal worm contamination in fresh vegetables. Consumption of raw vegetables can be a source of intestinal worm infection, thereby increasing the prevalence of foodborne diseases. This type of research is analytic observational with a cross-sectional study design. Samples in the form of fresh vegetables were taken from fresh vegetable traders in Pakusari District, Jember Regency and then processed by the sedimentation method and then observed using a microscope to identify intestinal protozoa. Food safety knowledge was assessed using a questionnaire, and the behavior of food traders was assessed by researchers using observation sheets. The bivariate analysis showed that proper handwashing behavior was related to intestinal worms in fresh vegetables (p<0,05). Other knowledge and behavior variables were unrelated to intestinal worms' presence. The intestinal worm species found is a type of Hookworm.
Karakterisasi Peptida Nterm-34 kDa Protein Saliva Nyamuk Aedes aegypti sebagai Kandidat Antigen Biomarker Risiko Transmisi Virus Dengue Secara In Silico Yunita Armiyanti; Nizar Fiska Bayu Agustian; Sheilla Rachmania
Jurnal Ilmu Kedokteran Vol 17, No 1 (2023): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26891/JIK.v17i1.2023.32-40

Abstract

Recently, a new biomarker of mosquito bite has been developed using human antibody response to mosquito salivary protein. One of the proteins that has been tested is salivary protein 34-kDa which has been refined to Nterm-34 kDa peptide. This study aimed to characterize Nterm-34 kDa salivary peptide as a potential antigen biomarker to asses Dengue virus transmission risk. The sequence Nterm-34 kDa was analyzed using several software to predict the characteristic of the peptide. BLASTP from Vectorbase and NCBI wa used to check the specificity of the peptide. IBIVU Praline was used to do multiple sequence alignment. IEDB (Bepipred and Kolaskar & Tongaonkar antigenicity prediction) was used to predict epitopes and antigenic properties, and lastly, ProtParam was used to predict physicochemical properties. The result showed that Nterm-34 kDa salivary peptide is specific to the  Aedes aegypti mosquito at the species level and has low conservation compared to other proteins in Aedes aegypti 34-kDa salivary protein family. Nterm-34 kDa is predicted to be antigenic and one of the epitopes. Nterm-34 kDa salivary peptide is predicted to have a molecular weight of 2,092 kDa, unstable, and hydrophilic. In conclusion, Nterm-34 kDa salivary peptide is predicted to have potential as an antigen biomarker for Dengue virus transmission.
Environmental Sanitation as Risk Factors for Intestinal Protozoa Infection among Stunted Children in Sugerkidul Village, Indonesia Hanu Neda Septian; Erma Sulistyaningsih; Angga Mardro Raharjo; Bagus Hermansyah; Wiwien Sugih Utami; Yunita Armiyanti
Al-Sihah : The Public Health Science Journal Volume 15, Nomor 1, January-June 2023
Publisher : Program Studi Kesehatan Masyarakat UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-sihah.v15i1.35378

Abstract

Stunting, a prevalent health issue in Indonesia, has been found to have a significant impact on the overall well-being of children, with a prevalence rate of 24.4%. This condition not only affects physical growth but also puts children at a heightened risk of various infections, including those caused by intestinal protozoa. Therefore, this study aimed to determine the relationship between personal hygiene parenting style and environmental sanitation with intestinal protozoa infection among stunted children in Sugerkidul Village, Jember District, Indonesia. The participants included were 30 stunted children. Questionnaires were used to collect data on personal hygiene parenting style and environmental sanitation, while microscopic stool examination was conducted to identify intestinal protozoa infection. The results showed that 26.7% of stunted children were infected with intestinal protozoa such as Giardia lamblia (13.3%), Blastocystis hominins (3.3%), and Cryptosporidium parvum (10%). Statistical analysis using Fisher's exact test showed a significant association between environmental sanitation and intestinal protozoa infection. However, there was no association between personal hygiene parenting style and this infection among stunted children in Sugerkidul Village. Therefore, to prevent intestinal protozoa infection, it is necessary to improve environmental sanitation.
Analysis of Milking Hygiene and Its Association to Staphylococcus Aureus Contamination in Fresh Cow Milk Nurus Saffana Yulianto; Yunita Armiyanti; Dini Agustina; Bagus Hermansyah; Wiwien Sugih Utami
JURNAL KESEHATAN LINGKUNGAN Vol. 15 No. 4 (2023): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v15i4.2023.275-282

Abstract

Introduction: Staphylococcus aureus foodborne disease is caused by the consumption of food contaminated with Staphylococcus aureus enterotoxins. Milk is a type of food that contains many nutrients but is susceptible to contamination by Staphylococcus aureus. Jember is one of the districts in East Java that produces cow milk. Previous research showed that the level of milk consumption in the community in 2018 was 3.1 kg/capita/year and 42% consumed pasteurized milk, which has lower quality and may still be contaminated with bacteria. The purpose of this study was to determine the relationship between milking hygiene and Staphylococcus aureus contamination. Methods: A cross-sectional study was conducted involving 36 cow milkers with traditional milking, selected by total sampling. The independent variable was milking hygiene and the dependent variable was Staphylococcus aureus contamination. Milking hygiene practice data were collected through direct observation using a questionnaire. Staphylococcus aureus contamination data were tested using Total Plate Count (TPC) and identified using Gram staining and catalase tests. Results and Discussion: The TPC test results showed that 61.1% of cow's milk fulfilled the Indonesian National Standard for Staphylococcus aureus contamination. There was a relationship between milking hygiene and Staphylococcus aureus contamination. The most influential milking hygiene was cage, udder, and teat hygiene. Conclusion: Staphylococcus aureus contamination did not meet the standards. Therefore, improving the sanitation and hygiene of cages, as well as udder and teat hygiene by dairy farmers, is necessary.
Spatial Map of Geohelminths Infection in Agricultural Communities and its Contamination in Soil of Jatian Village, Jember Regency Yunita Armiyanti; Nanda Nuramna; Muhammad Afiful Jauhani; Bagus Hermansyah; Wiwien Sugih Utami
JURNAL KESEHATAN LINGKUNGAN Vol. 15 No. 4 (2023): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v15i4.2023.309-316

Abstract

Introduction: Geohelminth infections are a neglected global health problem. Data from the World Health Organization (WHO) in 2020 show that more than 24% of the world population is infected by geohelminths. The condition of the agricultural soil, which tends to be loose and moist, supports the development of the infective form of the geohelminth. This makes agricultural communities susceptible to geohelminth infections. This study aimed to determine a spatial map of geohelminth infection in agricultural communities and its contamination in the soil of Jatian Village, Jember Regency. Methods: This study used an observational analytical design and a spatial analysis approach. Sampling was performed using a random sampling method that included 43 samples. Data were obtained by examining soil and stool samples and the coordinates of the sampling locations. Data were analyzed using spatial analysis. Results and Discussion: The results of this study indicated that the prevalence of geohelminth infection in agricultural communities was 23.3%, and its contamination in the soil was 6.98%. Hookworms caused infections among respondents, and contamination in the ground was caused by Hookworms and Strongyloides stercoralis. Clustering analysis results showed that geohelminth infection formed two secondary clusters. Spatial autocorrelation and buffer analysis showed clustering of geohelminth infections within the buffer range (<100 m), indicating that the infection spreads more easily within the cluster. Conclusion: The spatial map showed the distribution pattern of clustered geohelminth infection cases and their contamination in soil within proximity, thereby increasing the risk of geohelminth transmission.
Hubungan perilaku hidup bersih dan sehat dengan kejadian infestasi Soil–transmitted Helminths pada pekerja perkebunan kopi Sumber Wadung Kabupaten Jember Ahmad Hasbi Al-Muzaky; Bagus Hermansyah; Enny Suswati; Yunita Armiyanti; Yudha Nurdian
Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Vol. 6 No. 1 (2019): Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universi
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/jkk.v6i1.109

Abstract

Infestasi Soil - Transmitted Helminths (STH) merupakan masalah kesehatan yang masih sering ditemukan di negara beriklim tropis, salah satunya di Indonesia. Hal ini dikarenakan perkembangan STH membutuhkan kondisi lingkungan yang hangat dan lembab, serta memerlukan media tanah untuk menjadi fase infektif yang disebut soil - transmitted helminth.Kondisi ini dapat ditemukan di lingkungan perkebunan yang sangat menguntungkan bagi perkembangan STH. Hal ini menyebabkan para pekerja perkebunan sangat beresiko terinfestasi oleh STH. Tujuan penelitian ini adalah mengetahui adanya hubungan perilaku hidup bersih dan sehat (PHBS) dengan kejadian infestasi STH pada pekerja perkebunan kopi. Jenis penelitian ini adalah penelitian analisis deskriptif dengan desain cross-sectional yang dilaksanakan pada bulan Desember 2018 hingga Januari 2019. Perkebunan yang dipilih adalah Perkebunan Kopi Sumber Wadung di Kabupaten Jember dengan jumlah sampel sebanyak 100 orang responden dan diberikan kuisioer PHBS. Pemeriksaan infestasi STH dilakukan dengan dua metode yaitu flotasi dan sedimentasi. Dari 100 responden, 28 pekerja (28%) terinfestasi oleh STH. Kemudian dilakukan uji analisis chi-square antara PHBS dengan kejadian infestasi STH dan didapatkan adanya hubungan antara PHBS dengan kejadian infestasi STH pada pekerja (<0,05).
Correlation of Soil Contamination and Incident of STH Infection in Sukokerto Village Sukowono District Jember Regency Bagus Hermansyah; Yunita Armiyanti; Wiwien Sugih Utami; Aulya Rahmi; Ayu Munawaroh Aziz
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 8 No 02 (2024): Qanun Medika Vol 08 No 02 2024
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v8i02.18731

Abstract

The most common worm infections are those caused by soil-transmitted helminths (STH) infection. Soil contamination is one of the causes of helminth transmission to humans. Poor environmental sanitation and personal hygiene facilitate the spread and transmission of STH. Jember is a district where the majority of the population lives in rural areas with poor sanitation access. This study aims to determine the correlation between soil contamination and incident of STH infection in Sukokerto Village, Sukowono District, Jember Regency. This cross-sectional analytic observational study was conducted from August 2022 to February 2023. The total of samples in this study was 44 fecal and soil around the house. The data obtained from the laboratory examination showed that the total fecal positive of STH was 11 or 25%, and soil was 13 or 29.5%. The results of the Chi-Square test analysis obtained ρ<0.05 (ρ=0.036, OR=4,457). The results showed a significant relationship between soil contamination and the incidence of STH infection in Sukokerto Village, Sukowo District, Jember Regency
Co-Authors Achmad Maududie Adinda Putri Yusri Amrina Adrial Adrial Ahmad Hasbi Al-Muzaky Ahmad Hasbi Al-Muzaky Ahmad, Shabrina Izzata Afiyati Ali Machrus Amrina, Adinda Putri Yusri Andriani, Made Prasanti Angga Mardro Raharjo Anisah Ardiana Anthia Ayu Nandira Ardyansyah, Rizky Robeth Aulya Rahmi Ayundasari, Asti Ayundasari, Asti Azham Purwandhono Azham Purwandhono Aziz , Ayu Munawaroh Aziz, Ayu Munawaroh Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Brillian Adexa Yudinda Cholis Abrori, Cholis Cicih Komariyah Desi Dwi Cahyani Diana Chusna Mufida Dicky Setiawan Dimas Aji Bimantara Dina Helianti Dini Agustina Drajad, Rahadinda Mutia Dhamar Dwita Aryadina Rachmawati Edi Widjajanto Elly Nurus Sakinah Elvia Rahmi Marga Putri Emilia Angelica Enny Suswanti ENNY SUSWATI Eny Nurmaida Epafroditus Sanjaya Adiguna Erfan Efendi Erma Sulistyaningsih Erma Sulistyaningsih Evi Umayah Ulfa Febrianti, Zahrah Fitri, Nadya Eka Fitriyah, Ainun Nadiratul Ghaiska Najma Amnur Hanu Neda Septian Heni Fatmawati Hermansyah, Bagus Hidayat Teguh Wiyono Himma, Alyatul Ida Srisurani Wiji Astuti Ika Rahmawati Sutejo Imtiyaz, Andi Hilman Irawan Fajar Kusuma Ivan Iqbal Baidowi Jauhani, M. Afiful Jauhar Firdaus Kartika Senjarini Kholifaturrohmah, Sakinatus Sariroh Kholifaturrohmy, Muhammad Rizqi Komang Yunita Wiryaning Putri Kristianningrum Dian Sofiana Laily Nurhayati Laksmi Indreswari Leersia Yusi Ratnawati Loeki Enggar Fitri M. Ali Shodikin Mash’ab Zirrun Al-Qomah Mei Diastutik, Vike Mirawati Moh Mirza Nuryady, Moh Mirza Muhammad Afiful Jauhani Muhammad Afiful Jauhani Muhammad Alif Taryafi Nabil Athoillah Nabila Putri Anissa Nanda Nuramna Nidya Husna Kholidah Nihayah Lukman Nisrina Salsabila Firmansyah Nita Alfianti Nizar Fiska Bayu Agustian Nuri Nurmaida, Eny Nurmaida, Eny Nurus Saffana Yulianto Pangestu, Ahmad Yudho Hadi Primadana, Aditya Rachmawati, Dwita Aryadina Rahardi, Verdian Rahardjo, Angga Mardro Rahmadhan, Adiz Dwiputra Rahmawati, Iis Rahmi, Aulya Ramadanti, Novi Awalia Ramadanti, Novi Awalia Ramadhani, Rafindra Ratnawati, Leersia Yusi Rezza Putri Mahartika Rini Riyanti Risyanto, Chusnul Aulia Arisny Putri Rohmah, Etik Ainun Rosita Dewi Sa'bani, Begawan Saiful Akhyar Lubis Salsabilla, Safira Putri Nur Saputra, Antonius Dwi Sheilla Rachmania, Sheilla Sugeng Setyo Utomo, Sugeng Setyo Sulistyaningsih, Erma Syamsuddin, Alif Fazduani Teguh Wahju Sardjono Teguh Wahju Sardjono Tohari, Achmad Ilham Widhiasari Normaningtyas Wiwien Sugih Utami Wulandari, Lindi Priyantika Tri Yoga Setyo Yudha Nurdian Yudha Nurdian Yudi Wicaksono Zahrah Febianti Zulaikha Rizqina Rahmawati