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Determinant of Water Source Contamination by Soil Transmitted Helminths in Rural Areas of Jember District Armiyanti, Yunita; Mash’ab Zirrun Al-Qomah; Angga Mardro Raharjo; Bagus Hermansyah; Wiwien Sugih Utami
BKM Public Health and Community Medicine The 12th UGM Public Health Symposium
Publisher : Universitas Gadjah Mada

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Abstract

Purpose: This study aims to identify STH eggs and larvae that contaminate water sources in rural areas of Jember and their risk factors. Method: This type of study is an observational analytic study with a cross-sectional approach. Samples from water sources in Mumbulsari and Sukowono districts were taken on inclusion criteria under flotation and sedimentation methods and totaled 56 samples. Respondents were interviewed on account of risk factors related to the presence of STH in water sources using a questionnaire and a total of 56 respondents. Result: The results showed that the common type of STH found was Hookworm (92.31%). The most polluted water source is river (53.84%). Based on the characteristics, the majority of respondents are female (67.86%), aged over 40 years (58.93%), have low education (57.15%), and have no job (51.79%). Found a relationship using chi-square between bowel habits and the presence of STH eggs and larvae (ρ > 0.05). Conclusion: This study proved that STH eggs and larvae can contaminate water sources such as rivers and wells The contamination is related to the habit of defecating not in latrines, so the important prevention for STH infection in water sources is to change the habit of defecation into a healthy habit.
Potensi Antimalaria Fraksi-Fraksi Daun Kembang Bulan (Tithonia diversifolia [Hemsley] A. Gray) Nuri; Wicaksono, Yudi; Rahardi, Verdian; Utami, Wiwien Sugih; Armiyanti, Yunita
Journal of Agropharmacy Vol. 1 No. 1 (2024)
Publisher : Faculty of Pharmacy, University of Jember

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Abstract

Kembang bulan (Tithonia diversifolia [Hemsley] A. Gray) has been used in traditional medicine to treat malaria. Previous research results showed that the ethanol extract of kembang bulan leaves had antimalarial activity in vivo with an ED50 of 113.39 mg/kgBW. Until now, the compounds with antimalarial activity in vivo are unknown. The initial stage that can be done to separate the compounds in the extract is fractionation. This study aims to separate the extract into fractions and test its antimalarial activity. Fractionation was carried out using a vacuum liquid chromatography method using a stationaryphase of silica gel GF254 and a mobile phase of n-hexane, chloroform and methanol in a gradient. In vivo antimalarial activity was tested using the Peter test method. Fractionation produces 5 fractions, each yield is 1.9; 2.1; 17.6; 60.8, and 17.6% respectively for fractions 1-5. The results of the antimalarial activity test for fractions 1-5 with a dose of 2 mg/kgBW showed inhibition of the growth of Plasmodium berghei respectively at 52.3 ± 1.4; 83.5±1.4; 74.6±1.2; 69.5±0.9; 44.8 ± 0.3%. Fraction 2 with the greatest resistance was tested further and showed an ED50 value of 0.52 mg/kgBW.
B-cell epitope prediction of MPB83 protein as a candidate for serodiagnostic antigen of bovine tuberculosis in human: In silico study Ahmad, Shabrina Izzata Afiyati; Aziz , Ayu Munawaroh; Armiyanti, Yunita; Mufida, Diana Chusna
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 15, No 2, (2024)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol15.Iss2.art4

Abstract

Background: Bovine tuberculosis (bTB) can be transmitted to humans by inhalation or consumption of incomplete pasteurized milk and dairy products derived from infected cows. Most cases of Mycobacterium tuberculosis (M. bovis) infection are resistant to tuberculosis (TB) drugs. The risk of death during treatment for bTB has been reported to be 2.55 times higher than for TB. However, the quality of diagnostic methods for bTB remains relatively low.Objective: We aim to evaluate the potential of the B-cell epitope of the MPB83 protein as a candidate bTB serodiagnostic antigen using an in silico approach. Methods: This study was a computer-based descriptive study using secondary data from the National Center for Biotechnology Information (NCBI) protein database. The MPB83 protein sequence was obtained from M. tuberculosis variant bovis AF2122/97 from the United Kingdom. We described the characteristics of the linear epitope of the M. bovis B-cell protein MPB83 by measuring antigenicity, molecular weight, instability index, and Grand Average of Hydropathy (GRAVY) score. The tools used in this study were IBIVU PRALINE, VaxiJen v2.0, IEDB, ExPASy ProtParam, Cluspro, and the PyMOL application.Results: We found an epitope that could be used for bTB serodiagnostic antigen with low conservation, the 106KLNPDVNLVDTLN118 epitope. It has the molecular weight, instability index, and GRAVY score of 1638.76 Da, -28.44, and -0.300, respectively. Epitopes with the best criteria were simulated by docking to human major histocompatibility complex (MHC) class II. Docking results showed that the lowest binding energy was -644.8 kcal/mol. Further analysis using the PyMOL application obtained 14 hydrogen bonds with bond distances ranging from 1.7 Å to 2.2 Å, all of which showed strong hydrogen bonds.Conclusion: The B-cell epitope of MPB83 protein sequence 106KLNPDVNLVDTLN118 has a potential serodiagnostic antigen candidate for human bTB.
Spatial analysis of tuberculosis cases among stunted toddlers in Rambipuji District, Jember Regency Utami, Wiwien Sugih; Amrina, Adinda Putri Yusri; Armiyanti, Yunita; Maududie, Achmad
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 15, No 2, (2024)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol15.Iss2.art8

Abstract

Background: The ongoing prevalence of tuberculosis (TB) and stunting presents significant health challenges, frequently localized within specific regions of Indonesia. Spatial analysis is essential in controlling infectious diseases like TB, as it identifies disease clusters and patterns of local TB spread within an area. Objective: This study aimed to analyze the distribution of TB cases among stunted children through spatial analysis. Methods: We used a cross-sectional analytical descriptive study. We interviewed parents of stunted children using a questionnaire. The diagnosis of TB was made based on the pediatric TB scoring table. Coordinate data of sample sites were obtained using a Geographic Information System (GIS), supported by risk factor analysis of TB. We then created a disease distribution map using the spatial analysis by Moran's Index and Nearest Neighbor Index (NNI) methods. Results: There were 15 childhood TB cases (8.2%) among stunted children in Rambipuji District. The spatial autocorrelation test using Moran's index showed that TB cases were clustered in Rambigundam village (Moran’s index 0.2364, p-value <0.05 and Z-score >1.96). The results of the NNI analysis showed dispersed results (p-value=0.000) in all villages. Conclusion: The distribution of childhood TB cases among stunted children in Rambipuji District is primarily random, except for Rambigundam Village, which shows a clustering of cases. According to the NNI methods, childhood TB cases among stunted children are spreading throughout all villages. These results underscore the need for initiatives to curb TB transmission, especially among stunted children, which should be targeted at all villages.
The Relationship between Sanitation and Personal Hygiene with Seroprevalence of Toxoplasmosis in Pregnant Women at Kaliwates and Banjarsengon Community Health Centers Nabila Putri Anissa; Yunita Armiyanti; Eny Nurmaida; Bagus Hermansyah; Erma Sulistyaningsih; Wiwien Sugih Utami
Journal of Agromedicine and Medical Sciences Vol. 11 No. 3 (2025)
Publisher : Faculty of Medicine, Universitas Jember

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Abstract

Toxoplasmosis is an infectious disease in animals that can be transmitted to humans. Sanitation and personal hygiene are risk factors for toxoplasmosis. Toxoplasmosis can be transmitted from pregnant women to their fetuses. Toxoplasmosis in pregnancy can cause pregnancy, premature birth, and congenital toxoplasmosis. According to the Jember District Health Office in 2023, access to proper sanitation and good hygienic behavior is still low. The purpose of this study was to analyze the relationship between sanitation and individual hygiene with toxoplasmosis seroprevalence in pregnant women at the Kaliwates and Banjarsengon Health Centers. This study used a crosssectional study type. The subjects of the study were 65 pregnant women at the Kaliwates and Banjarsengon Health Centers who were taken using the accidental sampling technique. The serological examination used the Toxoplasma IgM and IgG ELISA kit from Calbiotech®. The results of this study showed that 77% of pregnantwomen were detected positive for toxoplasmosis. The majority of pregnant women have good sanitation and personal hygiene categories of 87.7% and 90.8%, respectively. Based on the results of Fisher's exact test, sanitation and personal hygiene are not related to toxoplasmosis seroprevalence (p>0.05). This study concludes that sanitation and personal hygiene of pregnant women at the Kaliwates and Banjarsengon Health Centers are not significantly related to toxoplasmosis seroprevalence.  
Gangguan Pertumbuhan Janin sebagai Dampak Menurunnya Kesehatan Gigi dan Mulut Ibu Hamil Mei Diastutik, Vike; Rahmawati, Iis; Armiyanti, Yunita
JI-KES (Jurnal Ilmu Kesehatan) Vol. 7 No. 2 (2024): JI-KES (Jurnal Ilmu Kesehatan)
Publisher : LPPM Universitas Hafshawaty Zainul Hasan Probolinggo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33006/jikes.v7i2.769

Abstract

Abstrak Angka kejadian karies gigi dan gingivitis sering terjadi pada wanita hamil. Sekitar 60 hingga 75% wanita hamil menderita gingivitis, mengabaikan kesehatan mulut dan gigi selama kehamilan, sehingga menyebabkan kerusakan gigi, radang gusi, plak gigi, dan periodontitis. Kondisi tersebut memiliki efek buruk pada ibu hamil dan bayinya. Tujuan penelitian untuk mengetahui pengaruh kesehatan gigi dan mulut ibu hamil terhadap pertumbuhan janin. Penelitian dilakukan di Kabupaten Probolinggo pada bulan September 2023. Penelitian ini merupakan penelitian observasional analitik dengan desain penelitian cross-sectional. Teknik pengumpulan data menggunakan kuesioner dan rekam medis/buku KIA dari Januari sampai Mei 2023. Variabel independent adalah kesehatan gigi dan mulut ibu hamil. Variabel dependent adalah pertumbuhan janin. Populasi yaitu semua ibu hamil sebanyak 21.212 orang. Sampling menggunakan simple random sampling. Sampel yaitu Sebagian ibu hamil sebanyak 96 orang. Analisis bivariat menggunakan chi-square. Hasil penelitian menunjukkan sebagian besar status kesehatan gigi dan mulut ibu hamil sehat sejumlah 67 ibu hamil (70%). Sebagian besar pertumbuhan janin normal yaitu 54 ibu hamil (56%). Ada pengaruh kesehatan gigi dan mulut ibu hamil terhadap pertumbuhan janin.Upaya yang dapat dilakukan yaitu tenaga kesehatan memberikan penyuluhan kepada ibu hamil tentang kesehatan gigi dan mulut yang juga dapat berpengaruh terhadap pertumbuhan janin. Kata kunci: kesehatan gigi dan mulut, kehamilan, pertumbuhan janin, tinggi fundus uteri (TFU)   Abstract Pregnant women are frequently at risk for gingivitis and dental cavities.  About 60 to 75% pregnant women suffer gingivitis, neglecting oral and dental health during pregnancy, resulting in tooth decay, gingivitis, dental plaque, and periodontitis. This condition has bad effects on expectant mother and babies. The aim research is determine effect expectant mother’s dental and oral health fetal growth. The research was conducted in Probolinggo Regency in September 2023. This research was analytical observational study with cross-sectional design. Data collection techniques used questionnaires and medical records/KIA books from January to May 2023. The independent variable was dental and oral health pregnant. The dependent variable fetal growth. There are 21,212 expectant mothers in the population. Basic random sampling is used in sampling. The sample consisted of 96 expectant mother. Bivariate analysis using chi-square.The majority of the study's participants, 67 expectant mother (70%), had good dental and oral health. Of the 54 expectant mother (56%), the majority had normal fetal growth. The oral and dental health of expectant mother affects the growth of the fetus. Health professionals might try to inform expectant mother about the relationship between dental and oral health and fetal development. Keywords: dental and oral health, pregnant, fetal growth, fundus uteri height (TFU)
High Toxoplasmosis Seroprevalence and Anemia Among Early-Mid Pregnant Women in Jember, Indonesia Wulandari, Lindi Priyantika Tri; Armiyanti, Yunita; Aziz, Ayu Munawaroh; Hermansyah, Bagus; Sulistyaningsih, Erma; Utami, Wiwien Sugih
Journal of Community Medicine and Public Health Research Vol. 6 No. 2 (2025): Journal Community Medicine and Public Health Research
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jcmphr.v6i2.68484

Abstract

Toxoplasmosis is an infection caused by the parasite Toxoplasma gondii, which can be transmitted through various routes. Toxoplasmosis in pregnant women may cause abortion and fetal disabilities. Decreased body immunity due to anemia can increase the risk of toxoplasmosis during pregnancy. The purpose of this study was to identify the characteristics of pregnant women and their association with anemia, determine the prevalence of anemia and toxoplasmosis infection in pregnant women, and investigate whether anemia is a risk factor for toxoplasmosis in pregnant women. This research is an analytical, observational study using a cross-sectional laboratory examination. The sample in this study consisted of blood collected from first- and second-trimester pregnant women at the Kaliwates and Banjarsengon Public Health Centers for hemoglobin analysis and toxoplasmosis serology. Accidental sampling was conducted from July to December 2024. The research data were presented in tabular form and analyzed using the Chi-square test. This study was conducted on 65 pregnant women. The number of pregnant women with anemia was 43% and the number of pregnant women who were positive for toxoplasmosis was 66%. A significant association exists between gestational age and anemia (p-value = 0,041). Bivariate analysis between anemia and T. gondii infection showed no significant association (p-value = 0.182). This study concludes that anemia is not proven to be a risk factor for toxoplasmosis infection in pregnant women. However, the cases of anemia and toxoplasmosis were high and require specific attention.
The Association between Environmental Sanitation and Soil Transmitted Helminths (STH) Coinfection of Tuberculosis Patients in Panti District, Jember Regency Nita Alfianti; Yunita Armiyanti; Bagus Hermansyah; Enny Suswati; Dini Agustina; Diana Chusna Mufida
Journal of Ners and Midwifery Vol 7 No 3 (2020)
Publisher : STIKes Patria Husada Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26699/jnk.v7i3.ART.p354-361

Abstract

Tuberculosis (TB) is a contagious infectious disease caused by the bacteria Mycobacterium tuberculosis. More than 80% of TB cases attack the pulmonary organs and the rest are extra-pulmonary TB. Indonesia is one of the countries with the high number of tuberculosis cases besides India, China, the Philippines, and Pakistan. Aside from being a country with a high TB incidence, Indonesia is also an endemic country for helminth infections, especially Soil-Transmitted Helminths (STH). The high prevalence of STH infections in Indonesia allows STH coinfection in TB patients to cause decreasing immunity, thus affecting the outcome of TB infection. STH infection is very closely related to environmental sanitation. The purpose of this study was to identify and determine the association of environmental sanitation risk factors to the coinfection of Soil-Transmitted Helminths (STH) in tuberculosis patients in Panti District, Jember Regency. This type of research was observational research with cross-sectional analytic design. Of the 49 TB patients who were undergoing treatment at the Panti Health Center, 32 people were willing to be respondents, but only 25 people collected stool samples. Fisher's test results showed that the incidence of intestinal co-infection in TB patients in Panti District, Jember Regency was 12% and was caused by two STH species, namely A. lumbricoides and Hookworm. Environmental sanitation in TB patients was mostly good, e.g 54.5% of respondents had good environmental sanitation, but there was no significant association between environmental sanitation and the incidence of intestinal co-infection in TB patients in Panti District, Jember Regency (p> 0.05).
Effect of micronutrient deficiency on protozoal infection in stunting toddler Utami, Wiwien Sugih; Ramadanti, Novi Awalia; Rachmawati, Dwita Aryadina; Ratnawati, Leersia Yusi; Armiyanti, Yunita; Hermansyah, Bagus
Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 12 ISSUE 4, 2024
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/ijnd.2024.12(4).233-241

Abstract

Background: Stunting is a developmental disorder caused by chronic inadequate nutritional intake in toddlers, leading to deficiencies in various nutrients, including iron and zinc. This can weaken toddlers' immunity, making them more vulnerable to infectious diseases, such as intestinal protozoa. An infected digestive tract can interfere with absorbing nutrients and reduce appetite, causing the infection to persist and become chronic. Chronic infection triggers the formation of pro-inflammatory cytokines that affect bone growth, hindering growth and causing stunting.Objectives: This study confidently investigates the correlation between iron and zinc micronutrient intake and intestinal protozoan infection in stunting toddlers in Jember District.Methods: The research is observational and cross-sectional, with 568 stunting toddlers aged 0-59 months in Jember District, East Java as study subjects. Iron and zinc intake were collected through 2x24-hour food-recall questionnaire interview and processed using the Nutrisurvey software. Protozoan infection status in toddler feces samples was identified using direct smear and modified Ziehl-Neelsen methods. The data analysis method chosen was the Spearman correlation test.Results: The study found that stunted toddlers in Jember District had mostly deficient micronutrient intake. The average iron intake is 2.56±3.06 mg and the average zinc intake is 1.8±1.47 mg. Protozoan infection was found in this study has a prevalence of 15.7%, with identified species including Entamoeba histolytica (3.3%), Giardia lamblia (2.6%), Cryptosporidium parvum (3.2%), Blastocystis hominis (5.3%), and mixed infections (1.2%). The statistical analysis confirms that there is no significant correlation between the intake of micronutrients iron (p=0.91;r=0.005) and zinc (p=0.76;r=0.013) and intestinal protozoan infection in stunting toddlers in Jember District.Conclusions: Based on the data, protozoan infection may be caused by multiple factors, such as parenting practices and family socioeconomics. However, improving nutrition by education and giving additional supplementation are crucial to reduce the prevalence of malnutrition in toddlers.
Spatial Map Of Hygiene Risk And Infection Of Soil-Transmitted Helminths In Stunting Toddlers In Kaliwates Yunita Armiyanti; Dimas Aji Bimantara; Azham Purwandhono; Achmad Maududie; Wiwien Sugih Utami; Bagus Hermansyah
Window of Health : Jurnal Kesehatan Vol 9 No 1 (January 2026)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/woh.vi.2595

Abstract

Soil-transmitted helminths remain major neglected tropical infections affecting approximately 24% of the global population, particularly in areas with poor sanitation and hygiene. Chronic infection may contribute to malnutrition and stunting in children. This study aimed to analyze the spatial distribution of soil-transmitted helminth infections and hygiene risk among stunted toddlers in Kaliwates District. A cross-sectional study was conducted from August 2023 to May 2024 involving 103 stunted toddlers. Stool samples were examined using sedimentation and flotation methods, and hygiene data were collected through caregiver interviews. Fisher’s exact test and Moran’s Index were applied. The infection prevalence was 8.7%, predominantly hookworm. No significant association was found between hygiene factors and infection (p>0.05). Spatial analysis indicated a random distribution pattern. These findings suggest that deworming programs and hygiene promotion should be maintained, and spatial mapping may support targeted interventions.
Co-Authors Achmad Maududie Adinda Putri Yusri Amrina Adrial Adrial Ahmad Hasbi Al-Muzaky Ahmad Hasbi Al-Muzaky Ahmad, Shabrina Izzata Afiyati Ali Machrus Amrina, Adinda Putri Yusri Angga Mardro Raharjo Anisah Ardiana Anthia Ayu Nandira Ardyansyah, Rizky Robeth Aulya Rahmi Ayundasari, Asti Ayundasari, Asti Azham Purwandhono Azham Purwandhono Aziz , Ayu Munawaroh Aziz, Ayu Munawaroh Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Brillian Adexa Yudinda Cholis Abrori, Cholis Cicih Komariyah Desi Dwi Cahyani Diana Chusna Mufida Dicky Setiawan Dimas Aji Bimantara Dina Helianti Dini Agustina Drajad, Rahadinda Mutia Dhamar Dwita Aryadina Rachmawati Edi Widjajanto Elly Nurus Sakinah Elvia Rahmi Marga Putri Emilia Angelica Enny Suswanti ENNY SUSWATI Eny Nurmaida Epafroditus Sanjaya Adiguna Erfan Efendi Erma Sulistyaningsih Erma Sulistyaningsih Erma Sulistyaningsih Evi Umayah Ulfa Febrianti, Zahrah Fitri, Nadya Eka Ghaiska Najma Amnur Hanu Neda Septian Heni Fatmawati Hermansyah, Bagus Hidayat Teguh Wiyono Himma, Alyatul Ida Srisurani Wiji Astuti Ika Rahmawati Sutejo Imtiyaz, Andi Hilman Irawan Fajar Kusuma Irawan Fajar Kusuma Ivan Iqbal Baidowi Jauhani, M. Afiful Jauhar Firdaus Kartika Senjarini Komang Yunita Wiryaning Putri Kristianningrum Dian Sofiana Laily Nurhayati Laksmi Indreswari Leersia Yusi Ratnawati Loeki Enggar Fitri M. Ali Shodikin Made Prasanti Andriani Mash’ab Zirrun Al-Qomah Mei Diastutik, Vike Mirawati Moh Mirza Nuryady, Moh Mirza Muhammad Afiful Jauhani Muhammad Afiful Jauhani Muhammad Alif Taryafi Muhammad Rizqi Kholifaturrohmy Nabil Athoillah Nabila Putri Anissa Nanda Nuramna Nidya Husna Kholidah Nihayah Lukman Nisrina Salsabila Firmansyah Nita Alfianti Nizar Fiska Bayu Agustian Nuri Nurmaida, Eny Nurmaida, Eny Nurus Saffana Yulianto Pangestu, Ahmad Yudho Hadi Primadana, Aditya Rachmawati, Dwita Aryadina Rahardi, Verdian Rahardjo, Angga Mardro Rahmadhan, Adiz Dwiputra Rahmawati, Iis Rahmi, Aulya Ramadanti, Novi Awalia Ramadanti, Novi Awalia Ramadhani, Rafindra Ratnawati, Leersia Yusi Rezza Putri Mahartika Rini Riyanti Risyanto, Chusnul Aulia Arisny Putri Rohmah, Etik Ainun Rosita Dewi Rosita Dewi Sa'bani, Begawan Saiful Akhyar Lubis Sakinatus Sariroh Kholifaturrohmah Salsabilla, Safira Putri Nur Saputra, Antonius Dwi Sheilla Rachmania Sheilla Rachmania, Sheilla Sugeng Setyo Utomo, Sugeng Setyo Sulistyaningsih, Erma Syamsuddin, Alif Fazduani Teguh Wahju Sardjono Teguh Wahju Sardjono Tohari, Achmad Ilham Widhiasari Normaningtyas Wiwien Sugih Utami Wulandari, Lindi Priyantika Tri Yoga Setyo Yudha Nurdian Yudha Nurdian Yudi Wicaksono Zahrah Febianti Zulaikha Rizqina Rahmawati