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All Journal Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Microbiology Indonesia Media Kedokteran Hewan Jurnal Medik Veteriner Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences
Ida Bagus Oka Winaya
Faculty Of Veterinary Medicine, Udayana University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Gambaran Histopatologi Hati dan Ginjal Babi Landrace yang Diberi Pakan Eceng Gondok dari Perairan Tercemar Timbal Ni Kadek Nining Laksmi Dewi; Ida Bagus Oka Winaya; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (823.61 KB) | DOI: 10.24843/bulvet.2017.v09.i01.p01

Abstract

The aim of this study is to determine the effect of water hyacinth (Eichornia crassipes) collected from Lead (Pb) polluted water in feed to histopathological changes in pig’s liver and kidney. A total of eight Landrace pigs were used in this study, consisted of four groups: pigs were fed without hyacinth (A), pigs were fed with 2.5% hyacinth (B), pigs were fed with 5.0% hyacinth (C), and pigs were fed with 7.5% hyacinth (D). The sample of dead pigs were dissected at the end of study to examined the liver and kidney. Both organs sample were immersed in a fixative solution of 10% neutral buffered formalin. The samples were then processed for tissue processing by using Hematoxillin-Eosin method before performing histophatological examination by using 100x, 200x and 400x magnificence under a microscope. Data were then analyzed descriptively by comparing the pathological changes in the liver and kidneys between groups. The results showed that the administration of water hyacinth (Eichornia crassipes) collected from Lead (Pb) polluted water causes a histopathological changes in the liver and kidneys of Landrace pigs. The changes include degeneration, necrosis and inflammation.
Histopatologi Ginjal Tikus Putih Akibat Pemberian Ekstrak Pegagan (Centella asiatica) Peroral Luh Putu Ratna Suhita; I Wayan Sudira; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 5 No.1 Pebruari 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.135 KB)

Abstract

Pegagan merupakan tanaman herbal  yang sering dimanfaatkan oleh masyarakat Indonesia sebagai obat alternatif untuk mengobati berbagai macam penyakit. Penelitian tentang toksisitas (studi histopatologi) tanaman pegagan pada ginjal belum pernah dilakukan. Adapun tujuan dari penelitian ini adalah untuk mengetahui adanya perubahan histopaotogi pada ginjal tikus putih setelah pemberian ekstrak pegagan (Centella asiatica) peroral. Tikus putih (Rattus norvegicus) dibagi secara acak menjadi lima kelompok, masing-masing kelompok berjumlah 6 ekor. Kelompok A sebagai control (placebo) yang diberi aquades peroral; kelompok B yang diberikan ekstrak pegagan 100 mg/kg bb (0,2 ml/ekor); kelompok C yang diberikan ekstrak pegagan dosis 200 mg/kg bb (0,4 ml/ekor); kelompok D yang diberikan 300 mg/kg bb (0,6 ml/ekor); dan kelompok E yang diberikan ekstrak pegagan dosis 400 mg/kg bb (0,8 ml/ekor). Nekropsi untuk pengambilan organ ginjal dilakukan pada hari ke-9. Jaringan ginjal selanjutnya diproses untuk pembuatan preparat histopatologi dengan pewarnaan Hemaktosilin Eosin (HE). Hasil pemeriksaan histopatologi pada ginjal tikus putih yang diberikan ekstrak pegagan, tidak ditemukan adanya degenerasi melemak, degenerasi hidrofik, dan nekrosis baik pada control (placebo) maupun pemberian dosis 0,2  ml; 0,4 ml; 0,6 ml; 0,8 ml. Hasil ini menunjukkan pemberian ekstrak pegagan (Centella asiatica) dengan rentang dosis 100 mg/kg bb sampai dengan dosis 400 mg/kg bb selama 9 hari, tidak menyebabkan gangguan histopatologi pada organ ginjal tikus putih (Rattus novegicus).
Perubahan Mikroskopis Dermatitis Kompleks pada Anjing yang Diberikan Terapi Minyak Nimba Dewa Odiec Purnawan; I Nyoman Suartha; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 14 No. 2 April 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1042.95 KB) | DOI: 10.24843/bulvet.2022.v14.i02.p01

Abstract

Complex dermatitis is inflammation of the skin caused by various types of agents such as fungi, ectoparasites, bacteria and congenital diseases that occur simultaneously so that the clinical signs shown are in the form of clinical combination of primary lesions and secondary lesions. The aim of the study was to observe changes in the microscopic skin of dogs with complex dermatitis patients after topical extract of neem oil. The sample consisted of 3 dogs that had moderate complex dermatitis. The three dogs were smeared with herbal extracts to the external part of the skin with lesions. Herbal extracts are given topically 2 times a day, morning and evening. On days 0, 7 and 15 a biopsy was performed on all three samples. Histopathological observations were carried out using a 100X and 400X binocular microscope. Histopathological examination shows changes in the form of hyperkeratosis, inflammatory cell infiltration in the form of basophils and neutrophils, ththosis, and ulcers. The results showed that administration of neural extracts affected dogs suffering from complex dermatitis or the administration of neem extracts can accelerate recovery in dogs suffering from complex dermatitis.
Daun Kelor Memperbaiki Histopatologi Hati Tikus Putih yang Mengalami Diabetes Melitus Ida Ayu Adhistania Pidada; Ni Luh Eka Setiasih; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (842.591 KB) | DOI: 10.24843/bulvet.2018.v10.i01.p08

Abstract

This study aims was to investigate the improvement of hepar histopathology experimental diabetic rats given moringa oleifera leaves ethanolic extract. 24 male white rats induced by a single dose STZ of 45mg/kg intraperitoneal to cause diabetes mellitus. After declared with diabetes disease, rats were divided into 6 groups consist of 1 a control group and a treatment group of 5 who were given ethanol extracts of leaves of the kelor, with a dose of 100, 200, 300, 400, and 500mg/kg, for 5 weeks. Rats then sacrificed and the hepar were taken for the histopathology preparations with Hematoxylin-Eosin staining (HE). Each preparation histopathology examined under a microscope in five visual fields at 400x magnification. The processed data is then analyzed statistically by test Non parametric Kruskal Wallis followed by Mann-Whitney test, when there is a significant difference (P<0.05). The results showed that the hepar has improved a result of the damage diabetes mellitus after given Moringa oleifera leaves ethanolic extract with a dose of 400 mg/kg.
Histopatologi Bronkiolus dan Pembuluh Darah Paru Mencit Jantan Pasca Terpapar Asap Rokok Elektrik Mia Monica; Anak Agung Ayu Mirah Adi; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.988 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p08

Abstract

This study aims to determine the histopathological changes of bronchioles and blood vessel of mice (Mus musculus) lung post exposed of electric cigarette smoke (vaping). This study was used 24 mice that were divided into two treatment groups. Group I (P0) as a control without electronic cigarette smoke exposured and group II (P1) consistied of 12 mice as a treatment group, were exprosure with electric cigarette smoke. This study used a complete randomized design (RAL) split in time pattern with three 1st, 2nd and 3rd post-treatment sampling times. Four mice of each group were taken at each observation time. Lung tissue was taken to make preparations and stained with Hematoxylin-eosin (HE) staining. The results showed that there were significant differences (P <0.05) between the treatment group and the control group, but the duration of exposure did not have an effect. Mucosal thickness in the treatment group was higher than in the control group (P <0.05). Vessel lung endothelins and thrombosis. It can be concluded, exposure to electric cigarette smoke can cause degeneration, cell necrosis and increase the mucosal thickness of the bronchioles. The vascular system experiences endotheliosis, endothelial cell necrosis and thrombosis
STUDI HISTOPATOLOGI HATI TIKUS PUTIH (Rattus norvegicus) YANG DIBERI EKSTRAK ETANOL DAUN KEDONDONG (Spondias dulcis G.Forst) SECARA ORAL I Putu Arya Adikara; Ida Bagus Oka Winaya; I Wayan Sudira
Buletin Veteriner Udayana Vol. 5 No. 2 Agustus 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.855 KB)

Abstract

Tanaman kedondong sering dimanfaatkan oleh masyarakat sebagai obat alternatif  untuk mengobati berbagai macam penyakit. Sedangkan penelitian tentang toksisitas daun kedondong pada hati belum pernah dilakukan. Dalam penelitian ini tikus putih (Rattus norvegicus) dibagi secara acak menjadi lima kelompok, masing-masing kelompok berjumlah 5 ekor. Kelompok A sebagai kontrol (placebo) yang diberi aquades peroral; kelompok B diberikan ekstrak daun kedondong 100 mg/kg bb (0,2 ml/ekor); kelompok C diberikan ekstrak daun kedondong 200 mg/kg bb (0,4 ml/ekor); kelompok D diberikan ekstrak daun kedondong 300 mg/kg bb (0,6 ml/ekor); kelompok E diberikan ekstrak daun kedondong 400 mg/kg bb (0,8 ml/ekor). Pemberian ekstrak daun kedondong dilakukan secara oral. Dilanjutkan dengan nekropsi pada hari ke-15 untuk pengambilan organ hati yang nantinya akan dibuat preparat hitopatologi.Hasil pemeriksaan histopatologi pada hati  tikus putih(Rattus norvegicus) yang diberikan ekstrak etanol daun kedondong, tidak ditemukan adanya perubahan seperti adanya infiltrasi sel radang, degenerasi melemak, degenerasi hidrofik dan nekrosis pada kelompok perlakuan K1, K2, K3, tetapididapatkan hasil peradangan ringan pada kelompok perlakuan K4 dan K5 pada 1 ekor tikus putih (Rattus norvegicus) dari 5 ekor yang diberi perlakuan pada tikus putih (Rattus norvegicus).
PENYULUHAN DAN PELAYANAN KESEHATAN TERNAK SAPI DI DESA KEMENUH GIANYAR I.K. Berata; I.M. Kardena; I.B.O. Winaya; N.N.W. Susari; I.W. Sudira
Buletin Udayana Mengabdi Vol 18 No 2 (2019): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.264 KB) | DOI: 10.24843/BUM.2019.v18.i02.p18

Abstract

Desa Kemenuh secara geografis sangat strategis sebagai kawasan agrowisata. Sektor peternakan, sebagai bagian dari program agrowisata, membutuhkan perhatian dan bimbingan berkelanjutan. Peternak sapi di desa Kemenuh masih rendah pengetahuan dan keterampilannya dalam memelihara ternak. Program ini bertujuan untuk menambah pengetahuan dan keterampilan petani. Dalam program penyuluhan disediakan waktu untuk diskusi antara tim penyuluh dan peternak. Setelah penyuluhan, dilanjutkan dengan pelayanan kesehatan berupa pengobatan ternak jika ada yang sakit, dan disertai dengan penjelasan tentang cara penanganan penyakit pada sapi. Sebanyak 52 ternak diperiksa dan diberi pengobatan gratis. Hasil pemeriksaan kesehatannya ditemukan sebanyak 10 ternak (19,23%) anemia, 4 (7,69%) kekurusan dan 1 (1,92%) menunjukkan demam. Gejala anemia cukup tinggi, mungkin karena infestasi cacing atau kekurangan protein dalam pakannya. Dapat disimpulkan bahwa peternak masih membutuhkan bimbingan dalam upaya meningkatkan pengetahuan dan keterampilan mereka sehingga nilai jual ternak mereka lebih menguntungkan. Perlu dilakukan tindak lanjut untuk memeriksa darah dan feses ternak untuk mencari penyebab anemia dan kekurusan. Kata kunci : Penyuluhan, pelayanan kesehatan, sapi, anemia, kekurusan
PEMBERANTASAN PENYAKIT DAN VAKSINASI HOG CHOLERA PADA TERNAK BABI DI DESA KELATING TABANAN BERATA BERATA; IB. OKA WINAYA; IGK. SUARJANA; IB. KADE SUARDANA
Buletin Udayana Mengabdi Vol 7 No 2 (2008): Volume 7 No.2 – September 2008
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

ABSTRACT Swines are the most important livestock to Balinese people because raising the swines could analogized with save the money.. Beside that balines people considering swine livestock also have economically value for religious ceremonial. Field observation indicated that some disease can threating the swine, one of these is Hog cholera. Hog cholera is a highly contagious viral disease of swine that occur in acute, a subacute, a chronic or a persistent form. In Order to protect the Bali cattle against this disease need conducting regularly vaccination. By vaccination can improvement cattle immunity against this disease. The objective this services is reducing economic loss due to Hog cholera infection and also increased farmes an understanding to this disease. .
Karakteristik Molekuler Virus Avian Orthoavulavirus 1 Genotipe VII yang Diisolasi dari Tabanan Bali (MOLECULAR CHARACTERISTIC OF AVIAN ORTHOAVULAVIRUS 1 GENOTYPE VII ISOLATED FROM TABANAN BALI) Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Nengah Wandia; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Anak Agung Keswari Krisnandika; Anak Agung Oka Wijaya
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.964 KB) | DOI: 10.19087/jveteriner.2019.20.4.593

Abstract

Newcastle disease (ND) is a very harmful avian disease, endemic in Indonesia and various parts of the world. The causative agent is ND virus or Avian orthoavulavirus 1 (AOAV-1). This virus is an RNA virus with wide genetic variation. Based on the genome length, it can be classified into AOAV-1 Class I and II. Class I are generally avirulent whereas Class II are consist of both virulent and avirulent viruses, currently there are 18 genotypes of the class II. To find out the molecular characteristics of AOAV-1 currently circulating in the field, isolation and identification of viruses from laying hens that was suspected ND from Tabanan Bali in 2017, was performed. The isolated viruses hereafter named as Tabanan1/ARP / 2017. A one-step RT-PCR reaction was carried out to amplify NP, F and HN gene fragments from the virus using three specific pairs of AOAV-1 primers. The obtained nucleotide sequences are then used in phylogenetic analysis. For phylogenetic analysis several strains of AOAV-1 from class II representing genotype I-VII as well as one strain from Class I were accessed from GenBank. From the analysis of the F gene nucleotide sequences, it was found that Tabanan 1 / ARP / 2017 is a genotype VII virus with an amino acid sequence at the F protein cleavage site is 112 R-R-Q-K-R-F117, a typical virulent strain. Phylogenetic analysis using nucleotide sequences NP and HN genes also positioned this isolate in genotype VII. At the nucleotide level, genetic distance with virulent isolates that was isolated in 2007 and 2010 were 8.26% and 1.08% while at the amino acid level were 5.26% and 0.64%. There were found mutations in amino acids at positions 107 and 108 of F protein.
Identifikasi Ekspresi Glikokonjugat pada Jaringan Limfonodus Sapi Bali yang Terinfeksi Virus Penyakit Jembrana Secara Eksperimental Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study is to compare glycoconjugate expression in the lymphonodes between bali cattleexperimentally infected with jembrana virus disease and non infected cattle. The biotinylated lectinsused in this study were Concavalia ensiformis (ConA), Arachis hypogaea (WGA), Triticum vulgaris (PNA),Ulex europeaus (UEA-1), and Sambucus nigra (SNA), respectively. The expression of lectin bindingglycoconjugate exhibit differences between the lymphonodes of infected and non infected cattle. Con A,WGA, and SNA were found be bound to lymphocytes whereas in infected cattle it was less intensive,repectively. More over, PNA and UEA were found strongly bound to the either lymphocytes of eitherinfected and non infected cattle.
Co-Authors Achoiro Wati Rasid Aida Lousie Tenden Rompis Anak Agung Ayu Mirah Adi Anak Agung Ayu Mirah Adi Anak Agung Bagus Bramardipa Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Anak Agung Sagung Kendran Aras, Fatmawati Astuti, Ni Made Widy Matalia Baiq Renny Kamaliani BERATA BERATA Bire, Ienoliski Rohi Brigietta Vincencia Simanihuruk Cahyani, Ni Kadek Devi Desak Wiga Puspita Dewi Dewa Odiec Purnawan Djara, Devita Vanessa Sukmawati DWI SURYANTO Dzikri Nurma'rifah Takariyanti Elyda . Fatmawati Aras Febrianty, Ni Made Dhea Guru, Yohana Cendyka Kartika Dewi Gusti Agung Ayu Putu Adriyati, Gusti Agung Ayu Gusti Ayu Yuniati Kencana Husain, Anugrah Fitria I Dewa Made Adhiwitana I Gede Made Andy Pratama I Gusti Agung Arta Putra I Gusti Agung Gede Putra Pemayun I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Narendra Putra1, I Kadek Parnayasa I Ketur Berata I Ketut Berata I Ketut Eli Supartika I Ketut Eli Supartika I Ketut Eli Supartika I Made Damriyasa I Made Kardena I Made Merdana I Made Merdana I Made Sukada i Nengah Wandia I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Arya Adikara I Wayan Gorda I Wayan Sudarmayasa I Wayan Sudira I Wayan Wirata I.K. Berata IB. KADE SUARDANA Ida Ayu Adhistania Pidada Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Sri Chandra Dewi Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Windia Adnyana Iwan Harjono Utama Iwan Haryono Utama Kadek Karang Agustina Komang Andika Purnama Komang, Nadia Eprillia Sary Darma Ni Kurnia, Ida Ayu Gintan Aristi Luh Made Sudimartini LUH PUTU AGUSTINI Luh Putu Ratna Suhita Lutviana, An'nisafitri M. Farhan Al Ma'arif Marissa Divia Dayanti Marissa Divia Dayanti Marson, Fransiska Gratia Sonita MAS DJOKO RUDYANTO Mia Monica Ni Kadek Eka Widiadnyani Ni Kadek Nining Laksmi Dewi Ni Komang Wijayani Ni Luh Aricahyani Ni Luh Eka Setiasih Ni Luh Putu Ratna Suhita Ni Nyoman Werdi Susari Ni Putu Trisna Asih NINING HARTANINGSIH Nur Baiti Palagan Senopati Sewoyo Palagan Senopati Sewoyo Pinem, Nuh Lasjuardi Purnama, Komang Andika Purnasari, Maria Elisabeth Putra, I Putu Werdikta Jayantika Putu Agus Trisna Kusuma Antara Putu Henrywaesa Sudipa Putu Intan Kusuma Wardani Rahmat Grahadi RAHMI MUSTABA Ryan Helmi Habibi Samsuri Samsuri Sartika, Nola Alfien Sartika, Nola Alfieni Sewoyo, Palagan Senopati Shafira Laili Aulia Sri Kayati Widyastuti Sugiyarto - Tampubolon, Yandri Putra Lumatas Theresia Ene Tjok Gde Oka Pemayun Tjokorda Sari Nindhia TRI KOMALA SARI Tyas Pandieka Yoga Velia Chyntia Victoria Wijaya, Anak Agung Gede Oka Wira, Amar Yeni Ratna Sari Yoshihiro Hayashi YUNNY DAMAYANTI Yustisia, Anggia