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All Journal Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Microbiology Indonesia Media Kedokteran Hewan Jurnal Medik Veteriner Journal of Applied Veterinary Science and Technology Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences Buletin Veteriner Udayana
Ida Bagus Oka Winaya
Faculty Of Veterinary Medicine, Udayana University
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Gambaran Sitologi Cairan Peritoneal dan Sinovial Itik Bali (CYTOLOGIC FIGURES OF PERITONEAL AND SYNOVIAL FLUIDS IN BALI DUCKS) Iwan Harjono Utama; Sri Kayati Widyastuti; Anak Agung Ayu Mirah Adi; I Gusti Made Krisna Erawan; Ida Bagus Oka Winaya; Ida Bagus Komang Ardana; Achoiro Wati Rasid; Tyas Pandieka Yoga
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aim was to observe cellular composition and morphology in peritoneal and synovial fluids ofbali ducks. This study used 50 ducks, which were composed of 21 males and 29 females aged 8 to 12 weeks.After being declared clinically healthy, the sample ducks were slaughtered and peritoneal fluid specimenfrom the abdominal cavity and the synovial fluid of joints tibiotarsal were collected. Then, in preparationfor the specimen, each of fluid was smeared and stained with Giemsa. Observations were made under amicroscope with a magnification of 1000 times and observations expressed in the average number of cellsper field of view observation. The results showed (duck population that most of the observed sample): inthe peritoneal fluid there was 36% eosinophils observed and 38% had heterophile cells with 0-3 cells perfield of magnification 1000x view. Similarly, 28% ducks had lymphocytes with 13-16 cells, and as much as28% of ducks had macrophage cells with 5- 8 cells in observation . In the synovial fluid, however, 64%sample ducks had monocytes, while 72% having lymphocytes with 1-18 cells each of view field withmagnification 1000x. In addition, in the synovial fluid, as much as 62% of ducks also observed had 1 to 3cells heterophiles and 60% ducks had eosinophils (polimorphonuclear cells) per view field observation.Macrophage cells that were found in the peritoneal fluid were mainly hyper granulated. It can be concludedthat the amount of mononuclear cells in both types of body fluids is likely more dominant compared to thenumber of polymorphonuclear cells.
Kadar Perasan Kunyit yang Efektif Memperbaiki Kerusakan Hati Mencit yang Dipicu Karbon Tetrachlorida (THE EFFICACY OF TURMERIC JUICE ON PATHOLOGICAL CHANGES OF THE MICE LIVER BY INDUCED CARBON TETRACHLORIDE) I Made Kardena; Ida Bagus Oka Winaya
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study on effect of various concentrations of turmeric juice on pathological changes mice liver hasbeen carried out. As many as 25 mice were used in this study and they were divided into 5 groups, i.e.: groupK-: 0.2 ml vegetable oil without CCl4; group K+: CCl4 without turmeric extract; group T1: CCl4 with 10%turmeric extract; group T2: CCl4 with 20% turmeric extract and group T3: CCl4 with 30% turmeric extract.All mice, except the K- group, were treated with 0.2 ml CCl4 orally for 7 days. On day-8 to day-29, the CCl4treated mice were given various concentrations of turmeric extract, i.e.: 10%, 20% and 30%; whereas, the KandK+ groups were given drinking water adlibitum. On day-30, all mice were sacrificed and their livertissues were collected and then processed to histopathological staining with haematoxylin-eosin. Theresult showed a clear evidence to suggest that turmeric extract can induce a better recovery compared tonon-treated mice. The improvement of pathological condition was characterized by reduction of pathologicalchanges found in the liver cells of mice, such as: hidrophic degeneration. fatty degeneration, and necrosis.More importantly, treatment with 30% of turmeric juice induced a better recovery than those with 10%and 20%.
Pelacakan Ekspresi Antigen Toxoplasma gondii secara Imuno(sito)histokimia (TRACKING EXPRESSION OF TOXOPLASMA GONDII ANTIGENS USING IMMUNO(CYTO)HISTOCHEMISTRY METHOD) Ida Ayu Pasti Apsari; Ida Bagus Oka Winaya; Tjokorda Sari Nindhia; Ida Bagus Ngurah Swacita
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.568 KB) | DOI: 10.19087/jveteriner.2017.18.4.535

Abstract

The research objective want to track the expression of Toxoplasma gondii antigenes in the heart of the free-range chicken with immuno(cyto)histochemistry technique. In the present study were examined three methods to track antigen T.gondii, first method direct microscopic examination of the heart digests, the second method to detect antigen of T.gondii with immunocytochemistry technique of the chicken heart digests and the third immunohistochemistry examination of the heart free-range chicken. The number of material samples examined were 100 heart free-range chicken. Direct microscopic examination of the heart digests free-range chicken to track the bradyzoite form (inside cyst). Examination by immuno(cyto)histochemistry technique keep track T.gondii an antigen expression on cells and heart muscle tissue. The results showed that the direct microscopic examination on the heart tissue unable to detect cyst and antigen T.gondii. Immunohistochemical examination successfully detected the expression of antigenes T.gondii and was found 2% (2/100) positive. It can be concluded that T.gondii antigen expression in the heart of range chicken could be detected by immunohistochemistry.
Establishment and Characterization of Benzo(a)pyrene-Induced Skin Tumor in Rats Palagan Senopati Sewoyo; Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Marissa Divia Dayanti; Anak Agung Bagus Bramardipa; I Ketut Eli Supartika
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.46 KB) | DOI: 10.19087/jveteriner.2022.23.1.1

Abstract

Quite a number of research on cancer therapy strongly require an animal model of cancer. One of the chemicals commonly used to induce cancer in animal models is benzo(a)pyrene due to its carcinogenic effects. This study aims were to describe the gross pathology of the tumor-induced by benzo(a)pyrene in an olive oil solution (w/v), identify the type of tumor histopathologically, and finally, determine the correlation between the duration of the rats experiencing tumor and it’s grade score. Tumor grade score is important to assess in order to determine tumor malignancy. This study consisted of 10 white rats (Rattus norvegicus) were given two treatments: a negative control treatment (K-) was injected with 0.1 mL of olive oil and a positive control treatment (K+) was injected with 0.1 mL of 0.3 % (w/v) benzo(a)pyrene in olive oil solution. Each treatment rats was kept in a cage and monitored regularly. When the tumors macroscopically appeared in the interscapular area and were observed until reached 4 cm in size, the rats were then sacrificed and necropsied. Tumors were observed for the gross pathology to examine the shape and color of them, then routinely processed for histopathological evaluation. The results showed that the tumors’ cells appeared to be round (1/5), irregular (2/5), and multilobular (2/5). Based on histopathological observation, the types of tumors observed were classical fibrosarcoma (2/5) and pleomorphic fibrosarcoma (3/5). There is a significant association between the duration of the rats experiencing tumors and the tumor grade. The longer the rats have tumors, the tumors tend to be more aggressive.
Pelacakan Secara Imunohistokimiawi Antigen Ekskretori-Sekretori pada Sapi Bali yang Terinfeksi Fasciola gigantica (IMMUNOHISTOCHEMICAL DETECTION OF EXCRET0RY-SECRETORY ANTIGENS IN BALI CATLLE INFECTED BY FASCIOLA GIGANTICA) Ida Bagus Oka Winaya; I Nyoman Mantik Astawa; I Made Damriyasa; Nyoman Sadra Dharmawan; I Ketut Berata
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In order to study the distribution of excretory-secretory (ES) F. gigantica in liver tissue of infected balicattle a research was establisihed using monoclonal antibodies (mAbs) againts ES antigens. Immortalmouse myeloma cells were fused with the lymphocytes derived from the spleen of mice that immunizedwith ES antigen. The mAbs were tested for their specificity by using enzyme linked immunosorbent assay(ELISA). Five specific mAbs againts ES antigens were isolated and two mAbs were used for immunodetectionof ES antigens in liver tissue of bali cattle. Immunohistochemical ES antigens were not detected in paraffinembeded tissue of negative confirmed fasciolosis samples. ES antigens was detected in hepatocytes andcytoplasm of bile duct epithelims in the bali cattle that infected with fasciolosis in moderate intensity.Therfore indicated that mAbs produced in this study are applicable for detecting ES antigens in bali cattleinfected by F. gigantica.
Immunological Detection of Avian Influenza Virus in Infected Ducks by Monoclonal Antibodies Against AIV-H5N1 NYOMAN MANTIK ASTAWA; IDA BAGUS OKA WINAYA; LUH PUTU AGUSTINI; NINING HARTANINGSIH
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.217 KB) | DOI: 10.5454/mi.1.3.5

Abstract

In order to establish a detection method for avian influenza virus (AIV) infection in ducks, monoclonal antibodies (MAbs) against the virus were produced. The virus used for the production of the monoclonal antibodies was AIV-H5N1 of Indonesian origin. Immortal mouse myeloma were fused with the lymphocytes derived from the spleen of mice immunized with the virus. The MAbs were tested for their specificity by enzyme linked immunosorbent assay (ELISA) and western blotting using formaldehyde inactivated virus and normal allantoic fluid as a negative control. Twelve MAbs which were specific against AIV were isolated and 8 of them were used for detecting of AIV antigen in duck’s tissues. AIV antigen was detected in paraffin embedded tissues of AIV-infected ducks by immunohistochemistry using MAbs. AIV antigen was not detected in ducks, which were confirmed to be AIV negative. In the infected ducks, high intensity of AIV infection was detected in proventricle gland and small intestine. The AIV antigen with a lesser intensity was also detected in lungs, spleen, and bursa of Fabricius, but hardly detected in muscle, brain, and several other issues. This study shows a clear evidence that MAbs produced in this study are applicable for use in immunological detection of AIV in infected duck tissues.
Dermatitis Suppurative Mengikuti Infeksi Tungau Demodeks dan Sarcoptes pada Anjing Kampung di Jalanan Kota Denpasar Ida Bagus Oka Winaya; Ida Bagus Windia Adnyana; I Ketut Berata; Ida Ayu Pasti Apsari
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.328

Abstract

Tujuan penelitian ini adalah untuk mengetahui penyebab dermatitis dan kerontokan rambut pada anjing jalanan di Kota Denpasar. Dilakukan pencatatan terhadap identitas 75 ekor anjing yang meliputi umur, ras, tipe rambut dan lokasi lesi pada permukaan tubuh. Sampel biopsi kulit dengan ukuran 6 mm diambil dari lesi kulit yang paling parah dengan tanda klinis hiperemia, gatal, adanya krusta, bersisik/scaling, kebotakan/alopecia. Pengambilan sampel biopsi dilakukan dengan terlebih dulu memberikan injeksi anestetik lokal dan dilanjutkan dengan pemberian spraying antiseptik setelah pengangkatan jaringan biopsi. Sampel jaringan kulit kemudian difiksasi dalam larutan neutral buffered formalin 10%. Setelah 24-48 jam dalam cairan NBF jaringan diembedding dalam blok paraffin dengan metode standar. Sedian dengan ketebalan 5 mikron selanjutnya diwarnai dengan hematoksilin-eosin (HE). Pemeriksaan histopatologi terhadap tujuh ekor anjing penderita dermatitis dan kerontokan rambut umur di bawah satu tahun ditemukan kumpulan tungau demodek di beberapa folikel bulu. Eksudat radang netrofil dan eritrosit juga ditemukan pada permukaan epidermis yang nekrosis. Pada pemeriksaan terhadap 26 ekor anjing penderita dermatitis dan kerontokan bulu umur di atas satu tahun ditemukan 24 ekor anjing murni terinfeksi tungau demodeks dan dua ekor terinfeksi tungau demodeks dan sarcoptes. Ditemukan juga adanya larva tungau demodeks di luar folikel rambut. Larva tungau perifolikel terlihat dikelilingi oleh netrofil pada area dermis nekrosis, sedangkan pada anjing yang terinfeksi oleh dua jenis tungau hanya ditemukan respons radang ringan di sekitar folikel rambut.
ASPEK PATOLOGIS INFEKSI PARVOVIRUS PADA ANAK ANJING DI KOTA DENPASAR Ida Bagus Oka Winaya; I Ketut Berata; AAA Mirah Adi; I Made Kardena
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.083 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2619

Abstract

Telah dilakukan penelitian mengenai aspek patologis infeksi parvovirus pada anak anjing di Denpasar. Sebanyak 80 ekor anak anjing telah diperiksa pada Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Udayana periode tahun 2011- 2012. Enam belas ekor diantaranya menunjukkan gejala klinis berak darah, depresi, anoreksia, dan dehidrasi. Sinyalemen mengenai umur, jenis kelamin, dan ras juga dicatat. Perubahan patologi anatomi secara signifikan berupa enteritis haemorrhagis et necrotican dapat ditemukan pada semua anak anjing penderita. Kongesti dan nekrosis ditemukan pada epikardium. Gambaran yang bersifat anemia ditemukan pada jaringan limpa, ginjal, hati, sedangkan paruparu mengalami hiperemia. Secara mikroskopis, pada usus halus mengalami hiperemia disertai dengan infiltrasi limfosit, villi terlihat atropi dan nekrosis pada kripta Lieberkuhn. Nekrosis limfosit (limfositolisis) ditemukan pada folikel limpa. Kongesti dan nekrosis pada otot jantung namun intranuclear inclusion bodies hanya ditemukan pada satu anak anjing penderita. Penebalan ditemukan pada septa alveoli sedangkan pada hati dan ginjal hanya ditemukan peradangan ringan. Secara klinis bentuk enteritis hemorhagis et necrotican selalu ditemukan pada anak anjing terinfeksi parvovirus di Kota Denpasar. ________________________
PERUBAHAN HISTOLOGIS DAN RESPONS IMUNITAS SAPI BALI YANG DIBERIKAN PAKAN CAMPURAN KONSENTRAT I ketut Berata; Ida Bagus Oka Winaya; I Made Kardena
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.275 KB) | DOI: 10.21157/j.ked.hewan.v6i2.307

Abstract

Penelitian ini bertujuan mengetahui perubahan histologis dan respons kekebalan sapi bali yang diberikan pakan campuran konsentrat. Sebanyak 12 ekor sapi bali betina berumur 2 tahun, dibagi secara acak atas 3 kelompok perlakuan yaitu kelompok I diberi pakan rumput, kelompok II diberi pakan campuran 2 bagian rumput dan 1 bagian konsentrat, dan kelompok III diberi pakan campuran 1 bagian rumput dan 1 bagian konsentrat. Sebelum diberi perlakuan, dilakukan uji respons kekebalan seluler dengan teknik uji methylthiazol tetrazolium (MTT). Uji respons kekebalan dilakukan kembali pada bulan ke-3 dan sesaat sebelum dilakukan nekropsi. Pada bulan ke-10 dilakukan nekrosi terhadap 2 ekor sapi dari masing-masing kelompok perlakuan. Sisa sapi dari masing-masing kelompok perlakuan dilanjutkan diberi perlakuan untuk penelitian lanjutan. Sapi yang dinekropsi diambil jaringan pencernaan yaitu usus, untuk selanjutnya diproses dalam pembuatan sediaan histologis. Sediaan histologis diwarnai dengan hematoksilin eosin (HE). Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan struktur histologis usus antara ketiga kelompok perlakuan sedangkan respons kekebalan seluler tertinggi pada kelompok yang diberi pakan konsentrat yang lebih banyak.
Patological Changes in Liver and Gall Bladder Of Bali Cattle Infected by Fasciolosis I Made Kardena; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; Ida Bagus Windia Adnyana; I Made Sukada; Kadek Karang Agustina; Putu Agus Trisna Kusuma Antara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p02

Abstract

Fascioliosis is a parasitic disease that infects ruminants and the disease is widely spread in the world. Fascioliosis caused by Fasciola hepatica and Fasciola gigantica that can cause macroscopic and microscopic lesions in the liver and gall bladder of bali cattle. Samples of bali cattle in Pesanggaran slaughter house that infected with fasciolosis were used in this study. The pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The parasite found in the liver and gall bladder, thickening of bile duct mucous were observed on macroscopic examination. However, in microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy and hyperplasia of the bladder epithelium were occurred.
Co-Authors Achoiro Wati Rasid Aida Lousie Tenden Rompis Alya Nita Shena Gayanti Anak Agung Ayu Mirah Adi Anak Agung Ayu Mirah Adi Anak Agung Bagus Bramardipa Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Anak Agung Sagung Kendran Aras, Fatmawati Astuti, Ni Made Widy Matalia Baiq Renny Kamaliani BERATA BERATA Bire, Ienoliski Rohi Brigietta Vincencia Simanihuruk Cahyani, Ni Kadek Devi Desak Wiga Puspita Dewi Dewa Odiec Purnawan Djara, Devita Vanessa Sukmawati DWI SURYANTO Dzikri Nurma'rifah Takariyanti Elyda . Fatmawati Aras Fauzia Hadista Anjani Febrianty, Ni Made Dhea Guru, Yohana Cendyka Kartika Dewi Gusti Agung Ayu Putu Adriyati, Gusti Agung Ayu Gusti Ayu Yuniati Kencana Gusti Ayu Yuniati Kencana Hapsari Mahatmi Husain, Anugrah Fitria I Dewa Made Adhiwitana I Gede Made Andy Pratama I Gede Yoga Sukya Sumantara I Gusti Agung Arta Putra I Gusti Agung Gede Putra Pemayun I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Kade Mahardika I Gusti Ngurah Narendra Putra1, I Kadek Parnayasa I Ketur Berata I Ketut Berata I Ketut Eli Supartika I Ketut Eli Supartika I Ketut Eli Supartika I Ketut Suarjana I Made Damriyasa I Made Dwinata I Made Kardena I Made Merdana I Made Merdana I Made Merdana I Made Sukada i Nengah Wandia I Nyoman Mantik Astawa I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Arya Adikara I Putu Cahyadi Putra I Putu Cahyadi Putra, I Putu Cahyadi I Wayan Gorda I Wayan Gorda I Wayan Sudarmayasa I Wayan Sudira I Wayan Wirata I.K. Berata IB. KADE SUARDANA Ida Ayu Adhistania Pidada Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Sri Chandra Dewi Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Windia Adnyana Iwan Harjono Utama Iwan Haryono Utama Kadek Karang Agustina Komang Andika Purnama Komang, Nadia Eprillia Sary Darma Ni Kurnia, Ida Ayu Gintan Aristi Luh Made Sudimartini LUH PUTU AGUSTINI Luh Putu Ratna Suhita Lutviana, An'nisafitri M. Farhan Al Ma'arif Marissa Divia Dayanti Marissa Divia Dayanti Marson, Fransiska Gratia Sonita MAS DJOKO RUDYANTO Mia Monica Ni Kadek Eka Widiadnyani Ni Kadek Nining Laksmi Dewi Ni Kadek Puspa Dewi Ni Komang Wijayani Ni Luh Aricahyani Ni Luh Eka Setiasih Ni Luh Putu Ratna Suhita Ni Nyoman Werdi Susari Ni Putu Trisna Asih NINING HARTANINGSIH Nur Baiti Palagan Senopati Sewoyo Palagan Senopati Sewoyo Pinem, Nuh Lasjuardi Purnama, Komang Andika Purnasari, Maria Elisabeth Putra, I Putu Werdikta Jayantika Putri, Dilyanti Maya Putu Agus Trisna Kusuma Antara Putu Henrywaesa Sudipa Putu Intan Kusuma Wardani Rahmat Grahadi RAHMI MUSTABA Ryan Helmi Habibi Samsuri Samsuri Samsuri Sartika, Nola Alfien Sartika, Nola Alfieni Sewoyo, Palagan Senopati Shafira Laili Aulia Shafira Laili Aulia Sri Kayati Widyastuti Sugiyarto - Tampubolon, Yandri Putra Lumatas Theresia Ene Tjok Gde Oka Pemayun Tjokorda Sari Nindhia Tono PG, I Ketut TRI KOMALA SARI Tyas Pandieka Yoga Velia Chyntia Victoria Widyasanti, Ni Wayan Helpina Wijaya, Anak Agung Gede Oka Wira, Amar Wuriandari, Paula Bintang Yasa, Gede Sastra Darma Yeni Ratna Sari Yeyen Agustianingsi Yolla Noviolita Yoshihiro Hayashi YUNNY DAMAYANTI Yustisia, Anggia