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All Journal Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Microbiology Indonesia Media Kedokteran Hewan Jurnal Medik Veteriner Journal of Applied Veterinary Science and Technology Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences Buletin Veteriner Udayana
Ida Bagus Oka Winaya
Faculty Of Veterinary Medicine, Udayana University
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Tracking Antigen Expression of Toxoplasma gondii in Mice by Immuno (Cyto) Histochemistry Ida Ayu Pasti Apsari; Ida Bagus Oka Winaya; Tjokorda Sari Nindhia; Ida Bagus Ngurah Swacita
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p08

Abstract

Mouse has been an animal model that is very sensitive towards the Toxoplasma gondii infection. This research infects 30 mice with free-range chicken heart inoculate and the detection of T. gondiiby immunohistochemistry on the infected mice. The purpose of this study is to track T.gondii antigen expression by immune(cyto)histochemistry in mice. Observation over mice was conducted over 10 weeks. After 10 weeks, the mice are examined for its peritoneal fluid by microscopic and immunohistochemistry examination. Continued by the examination of internal organs to track the expression of T.gondii antigens by immunohistochemistry. The study result showed microscopic examinations on peritoneal fluid did not found any cyst or tachyzoites form of T.gondii. Examination of peritoneal fluid by immunohistochemistry was found to detect expressions of T.gondii antigen. Examinations of the mice’s internal organs detected expressions of T.gondii antigen only in the brain by immunohistochemistry examinations.
Mammary Tumors in Dogs, Recent Perspectives and Antiangiogenesis as a Therapeutic Strategy: Literature Study Sewoyo, Palagan Senopati; Adi, Anak Agung Ayu Mirah; Winaya, Ida Bagus Oka; Wirata, I Wayan
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.271-287

Abstract

A particular type of tumor that is frequently detected in female dogs who are sexually active is a mammary tumor. Neoplasia results from DNA-based alterations in cell cycle regulating genes. The mammary gland is prone to the formation of tumors due to its dynamic structure. The development of this tumor is supported by numerous variables. It has been recently discovered that there is substantial evidence linking the BRCA2 gene to the process of cancer. Standard examination techniques, such as fine needle aspiration, histopathology, and immunohistochemistry, are used along with ancillary tests to determine the tumor type and degree of malignancy. The primary treatment option for malignant tumors is surgical resection followed by adjuvant chemotherapy; benign tumors necessitate surgical resection as well. Adjuvant therapy options include hormone therapy and non-steroidal anti-inflammatory medications. Tumor tissue undergoes angiogenesis as it grows and develops to accommodate the abundant supply of nutrients. Therefore, angiogenesis-inhibiting therapies can be utilized to halt the growth of tumor cells. A number of antiangiogenic medications are now being studied in clinical settings on humans, and several more are undergoing trials on animals. In addition to pharmaceuticals, viruses may be used as a therapeutic to block tumor angiogenesis.
Sebaceous Adenoma in a Geriatric Poodle Dog: A Case Report Winaya, Ida Bagus Oka; Adi, Anak Agung Ayu Mirah; Sudimartini, Luh Made; Merdana, I Made; Sudipa, Putu Henrywaesa; Pemayun, I Gusti Agung Gde Putra; Sewoyo, Palagan Senopati
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.413-419

Abstract

A 14-year-old black male Poodle was brought to the Animal Teaching Hospital, Udayana University by its owner with clinical signs of frequent licking of its left front paw. Upon examination, red bumps were observed on the left front leg, accompanied by small, round black spots scattered on the dorsal side of the body. Additionally, black nodules were present on the lower eyelids and hind limbs. Surgical intervention was undertaken to excise the tumor mass, with the animal under anesthesia induced by ketamine at 5 mg/kg BW intravenously. The reddish nodule was excised by performing an elliptical incision at the base of tumor. Postoperatively, the animal received an antibacterial injection comprising ceftriaxone and tazobactam at 25 mg/kg BW intramuscularly and antiseptic wound dressing for supportive care. Microscopic examination revealed neoplastic cells arranged into lobules of varying sizes and shapes within the tumor mass. These lobules consisted of differentiated sebocytes and basaloid cells. At the periphery of the neoplastic lobules, the basaloid cells displayed several layers and exhibited invasion with moderate anisocytosis. The mitotic index was no more than ten cells in one field of view. Based on these histopathological features, the tumor was confirmed to be a sebaceous adenoma. After a 10-month follow-up period, there were no signs of tumor recurrence observed.
Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models Marson, Fransiska Gratia Sonita; Sewoyo, Palagan Senopati; Astawa, I Nyoman Mantik; Adi, Anak Agung Ayu Mirah; Suardana, Ida Bagus Kade; Winaya, Ida Bagus Oka; Berata, I Ketut
Media Kedokteran Hewan Vol. 36 No. 1 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i1.2025.13-20

Abstract

c-Jun is often found to be overexpressed in various cancers, so this gene might be a target for cancer therapy. Newcastle disease virus (NDV) is recognized for its oncolytic properties and potential as a cancer virotherapy agent, with various mechanisms reported to trigger cancer cell death. This study aimed to assess the c-Jun expression in rat mammary cancer models. Rat mammary cancer models were categorized into two treatment groups: the control group (C) and the virotherapy group (V). Group C was administered with 0.5 cc of sterile PBS, while group V received 7 log 2 HAU per 0.5 cc of the Indonesian NDV field isolate Tabanan-1/ARP/2017 intratumorally. The treatment was carried out for four days in a row. Two weeks after treatment, all rats were humanely euthanized, and mammary cancer tissues were excised for further examination. Mammary cancer tissues were examined histopathologically and analyzed using immunohistochemistry to determine intranuclear c-Jun expression, quantified by the H-Score. The results demonstrated that NDV significantly reduced c-Jun expression. It can be inferred that NDV Tabanan-1/ARP/2017 holds potential as a mammary cancer therapy agent by reducing c-Jun expression. This finding is considered novel, as there have been no previous reports of decreased c-Jun expression following virotherapy with NDV.
PERUBAHAN HISTOLOGIS DAN RESPONS IMUNITAS SAPI BALI YANG DIBERIKAN PAKAN CAMPURAN KONSENTRAT Berata, I ketut; Winaya, Ida Bagus Oka; Kardena, I Made
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.307

Abstract

Penelitian ini bertujuan mengetahui perubahan histologis dan respons kekebalan sapi bali yang diberikan pakan campuran konsentrat. Sebanyak 12 ekor sapi bali betina berumur 2 tahun, dibagi secara acak atas 3 kelompok perlakuan yaitu kelompok I diberi pakan rumput, kelompok II diberi pakan campuran 2 bagian rumput dan 1 bagian konsentrat, dan kelompok III diberi pakan campuran 1 bagian rumput dan 1 bagian konsentrat. Sebelum diberi perlakuan, dilakukan uji respons kekebalan seluler dengan teknik uji methylthiazol tetrazolium (MTT). Uji respons kekebalan dilakukan kembali pada bulan ke-3 dan sesaat sebelum dilakukan nekropsi. Pada bulan ke-10 dilakukan nekrosi terhadap 2 ekor sapi dari masing-masing kelompok perlakuan. Sisa sapi dari masing-masing kelompok perlakuan dilanjutkan diberi perlakuan untuk penelitian lanjutan. Sapi yang dinekropsi diambil jaringan pencernaan yaitu usus, untuk selanjutnya diproses dalam pembuatan sediaan histologis. Sediaan histologis diwarnai dengan hematoksilin eosin (HE). Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan struktur histologis usus antara ketiga kelompok perlakuan sedangkan respons kekebalan seluler tertinggi pada kelompok yang diberi pakan konsentrat yang lebih banyak.
Landrace Swine Mortality Due to Colibacillosis in Conventional Farming Yasa, Gede Sastra Darma; Tono PG, I Ketut; Winaya, Ida Bagus Oka; Apsari, Ida Ayu Pasti; Sari, Tri Komala
Journal of Applied Veterinary Science And Technology Vol. 6 No. 2 (2025): October 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V6.I2.2025.178-184

Abstract

Background: Colibacillosis, caused by Escherichia coli infection, is a major health issue in pig farming, leading to high morbidity and mortality rates. This report describes a case that occurred on a conventional pig farm in Puhu Village, Gianyar District, Bali. Purpose: This study aimed to determine the cause of death in a 43 day old Landrace pig exhibiting clinical signs including brown watery diarrhea, dehydration, and weight loss. Case(s): A necropsy was conducted at the Veterinary Pathology Laboratory, Faculty of Veterinary Medicine, Udayana University. Bacteriological examination was performed by culturing organ samples on Nutrient Agar and Eosin Methylene Blue Agar, followed by biochemical testing using Triple Sugar Iron Agar and Sulfide Indole Motility media, among others. Parasitological examination was carried out using the fecal float concentration method. Case Management: Epidemiological assessment revealed morbidity, mortality, and case fatality rates of 1.89%, 0.63%, and 33.33%, respectively. Gross pathological findings included intestinal and gastric dilation and hemorrhage, intestinal edema, and lesions in the brain, trachea, lungs, heart, liver, and kidneys. Histopathological examination revealed localized intestinal congestion, generalized cardiac congestion, and inflammatory cell infiltration in the intestinal mucosa. Bacteriological analysis confirmed Escherichia coli with alpha-hemolytic activity on blood agar. Eimeria spp. oocysts were not detected in the fecal samples. Conclusion:  The pig’s death was attributed to colibacillosis caused by Escherichia coli infection. This case highlights the importance of early detection, strict biosecurity measures, and proper sanitation practices to prevent disease outbreaks in conventional pig farming.
CASE OF AVIAN INFLUENZA IN BROILER CHICKEN AT JATILUWIH VILLAGE Alya Nita Shena Gayanti; I Gusti Ngurah Kade Mahardika; Ida Bagus Oka Winaya; I Gusti Ketut Suarjana; Ida Ayu Pasti Apsari
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p07

Abstract

Avian Influenza (AI) is still endemic in Bali. This disease is very dangerous and deadly, is zoonotic in birds and humans and causes high economic losses. AI disease in poultry is caused by the Influenza virus type A. The aim of the examination is to identify the agent that caused the death of the chicken in the case to determine a definite diagnosis. The case chicken sample was a 29 day old broiler chicken from a farm in Jatiluwih Village, Tabanan Regency, Bali. Clinical symptoms of chickens include: weak chickens, shaking, shortness of breath, no appetite for eating or drinking, dull feathers, pale bluish combs, runny discharge from the beak, and white-brown watery feces. Chicken death occurred 2 days after clinical symptoms appeared. After the chicken dies, a necropsy is carried out and the samples are examined in the histopathology, virology, bacteriology and parasitology laboratories to determine the agent that caused the death. Histopathological examination showed that all organs had lesions. The results of the HA/HI test showed that the chicken was a positive case of being infected with the Avian Influenza virus. Bacterial infection testing in the media test, selective test, primary test, secondary test and confectionery test identified the presence of Staphylococcus sp bacteria. in the liver and lungs. The results of fecal examination during parasite examination using native and concentration methods did not reveal any worm eggs or protozoa. It was concluded that the case chicken was infected with Avian Influenza with secondary bacterial infection, namely Staphylococcus sp. It is recommended that breeders improve biosecurity and carry out routine and appropriate vaccinations to prevent Avian Influenza disease.
SUSPECTED OF TRANSMISSIBLE GASTROENTERITIS AND COLIBACILLOSIS IN PIGLET AT TEGAL SUCI VILLAGE FARM, SEBATU DISTRICT, GIANYAR REGENCY, BALI Shafira Laili Aulia; I Ketut Suarjana; Ida Bagus Oka Winaya; Gusti Ayu Yuniati Kencana; I Made Dwinata
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p17

Abstract

The case pig originated from a farm in Tegal Suci Village, Sebatu District, Gianyar Regency, Bali. This case study review aims to establish the diagnosis of death in a 45-day-old female pig using anamnesis, epidemiological history, clinical symptoms, anatomical pathology examination, histopathology, and laboratory tests. The observed symptoms in the case pig included anorexia, weakness, and diarrhea. Necropsy results showed hemorrhaging in almost all organs of the case pig. Histopathological examination revealed lesions in the intestine, suggesting a Transmissible Gastroenteritis Virus (TGEV) infection, specifically intestinal villous atrophy. Bacteriological examination successfully isolated Escherichia coli bacteria in the lungs, liver, and heart. Fecal examination in the parasitology laboratory showed no evidence of worm eggs or protozoan infections. Based on a series of tests conducted, it can be concluded that the case pig is suspected to have been infected with TGEV and E. coli. Further testing is needed to investigate the co-infection of Transmissible Gastroenteritis Virus and E. coli in pigs to determine the appropriate treatment for severe diarrhea in pigs.
AFRICAN SWINE FEVER, STREPTOCOCCOSIS AND COCCIDIOSIS IN PIG AT PUHU VILLAGE, PAYANGAN, GIANYAR Yeyen Agustianingsi; I Nyoman Mantik Astawa; Hapsari Mahatmi; Ida Bagus Oka Winaya; I Putu Cahyadi Putra
Buletin Veteriner Udayana Bul. Vet. Udayana. October 2024 Vol. 16 No. 5
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i05.p10

Abstract

This case study aims to discuss the anatomical pathology, histopathology, and laboratory examination results to obtain a definitive diagnosis of the disease affecting 4 month old pigs from Puhu Village, Gianyar. Data collection in the form of indications, anamnesis, epidemiological and laboratory examinations for further descriptive qualitative analysis. Clinical signs of pig include anorexia, weakness, diarrhea, fever (39.7oC), multifocal cyanosis of skin, severe neurological symptoms. Epidemiological data shows morbidity reaching 100%, mortality 78.94%, and case fatality rate 78.94%. An anatomical pathology examination was carried out through necropsy procedure and found hydropericardium, hemorrhage in the heart, brain, intestines, stomach, lungs, liver, kidneys had pthechie, while spleen changed color to dark and swollen, organ samples were collected for histopathology examination. Histopathological examination begins with histological preparations using Hematoxylin-Eosin (HE) staining. Examination were carried out by looking at the changes descriptively using binocular light microscope with 100x, 400x magnification. Histopathological examination showed that the brain (hemorrhagic meningitis), lungs (hemorrhagic interstitial pneumonia), heart (myocardial edematous), spleen (hemorrhagic spleen) and small intestine (necrotizing enteritis), large intestine (hemorrhagic et necrotizing colitis). In histology preparations, inflammation was found and dominated by lymphocyte cells and a few neutrophil cells. In bacteriological examination, Samples were isolated using Nutrient Agar, Blood Agar media. Bacterial identification then carried out using the Gram stain test, catalase test, Triple Sugar Iron Agar, Sulfide Indole Motility, Methyl Red Voges Proskauer, Simmon Citrate Agar, glucose test. Bacteriological examination confirmed the presence of Streptococcus suis bacterial infection in the brain, lungs, spleen. Parasitological examination of feces was carried out using native, floating and sedimentation methods, Eimeria spp. oocysts were found and calculated using quantitative methods with McMaster technique as 30,200 oocysts/gram. Based on all data and laboratory examination results, it was concluded that the pig was infected with African swine fever, Streptococcus suis and Eimeria spp. In pig farming management, strict biosecurity and sanitation must be implemented to minimize the risk of contamination by disease agents.
PATHOGENIC ESCHERICHIA COLI BACTERIA INFECTION IN LANDRACE PIGLETS IN ANTIGA KELOD, MANGGIS, KARANGASEM Yolla Noviolita; I Gusti Ketut Suarjana; Ida Bagus Oka Winaya; Ida Bagus Made Oka; Ida Bagus Kade Suardana
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p03

Abstract

Colibacillosis is a disease caused by pathogenic Escherichia coli bacteria. Infected piglets experience weight loss, stunted growth and if not treated immediately will cause death. A 16-day-old Landrace piglet died with clinical signs of weakness and yellowish white diarrhoea for 4 days. The farmer's population was 25 pigs. Epidemiology showed morbidity of 8%, mortality of 4%, and case fatality rate of 50%. Anatomical pathology examination revealed distended intestines with bleeding and hyperaemic lungs. Bacterial culture of lung and intestinal organs showed positive results of gram-negative Escherichia coli bacteria. The results of parasitological examination of native, sedimentation and floating methods were negative for protozoan and helminth infections. Histopathology showed congestion, haemorrhage and neutrophil-dominated inflammatory cell infiltration in the intestines and lungs, while the brain, heart and liver were congested. Based on all laboratory examination results, the case pig was diagnosed with colibacillosis. Improved sanitation and biosecurity of the barn and barn environment needs to be routinely carried out.
Co-Authors Achoiro Wati Rasid Aida Lousie Tenden Rompis Alya Nita Shena Gayanti Anak Agung Ayu Mirah Adi Anak Agung Ayu Mirah Adi Anak Agung Bagus Bramardipa Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Anak Agung Sagung Kendran Aras, Fatmawati Astuti, Ni Made Widy Matalia Baiq Renny Kamaliani BERATA BERATA Bire, Ienoliski Rohi Brigietta Vincencia Simanihuruk Cahyani, Ni Kadek Devi Desak Wiga Puspita Dewi Dewa Odiec Purnawan Djara, Devita Vanessa Sukmawati DWI SURYANTO Dzikri Nurma'rifah Takariyanti Elyda . Fatmawati Aras Fauzia Hadista Anjani Febrianty, Ni Made Dhea Guru, Yohana Cendyka Kartika Dewi Gusti Agung Ayu Putu Adriyati, Gusti Agung Ayu Gusti Ayu Yuniati Kencana Gusti Ayu Yuniati Kencana Hapsari Mahatmi Husain, Anugrah Fitria I Dewa Made Adhiwitana I Gede Made Andy Pratama I Gede Yoga Sukya Sumantara I Gusti Agung Arta Putra I Gusti Agung Gede Putra Pemayun I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Kade Mahardika I Gusti Ngurah Narendra Putra1, I Kadek Parnayasa I Ketur Berata I Ketut Berata I Ketut Eli Supartika I Ketut Eli Supartika I Ketut Eli Supartika I Ketut Suarjana I Made Damriyasa I Made Dwinata I Made Kardena I Made Merdana I Made Merdana I Made Merdana I Made Sukada i Nengah Wandia I Nyoman Mantik Astawa I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Arya Adikara I Putu Cahyadi Putra I Putu Cahyadi Putra, I Putu Cahyadi I Wayan Gorda I Wayan Gorda I Wayan Sudarmayasa I Wayan Sudira I Wayan Wirata I.K. Berata IB. KADE SUARDANA Ida Ayu Adhistania Pidada Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Sri Chandra Dewi Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Windia Adnyana Iwan Harjono Utama Iwan Haryono Utama Kadek Karang Agustina Komang Andika Purnama Komang, Nadia Eprillia Sary Darma Ni Kurnia, Ida Ayu Gintan Aristi Luh Made Sudimartini LUH PUTU AGUSTINI Luh Putu Ratna Suhita Lutviana, An'nisafitri M. Farhan Al Ma'arif Marissa Divia Dayanti Marissa Divia Dayanti Marson, Fransiska Gratia Sonita MAS DJOKO RUDYANTO Mia Monica Ni Kadek Eka Widiadnyani Ni Kadek Nining Laksmi Dewi Ni Kadek Puspa Dewi Ni Komang Wijayani Ni Luh Aricahyani Ni Luh Eka Setiasih Ni Luh Putu Ratna Suhita Ni Nyoman Werdi Susari Ni Putu Trisna Asih NINING HARTANINGSIH Nur Baiti Palagan Senopati Sewoyo Palagan Senopati Sewoyo Pinem, Nuh Lasjuardi Purnama, Komang Andika Purnasari, Maria Elisabeth Putra, I Putu Werdikta Jayantika Putri, Dilyanti Maya Putu Agus Trisna Kusuma Antara Putu Henrywaesa Sudipa Putu Intan Kusuma Wardani Rahmat Grahadi RAHMI MUSTABA Ryan Helmi Habibi Samsuri Samsuri Samsuri Sartika, Nola Alfien Sartika, Nola Alfieni Sewoyo, Palagan Senopati Shafira Laili Aulia Shafira Laili Aulia Sri Kayati Widyastuti Sugiyarto - Tampubolon, Yandri Putra Lumatas Theresia Ene Tjok Gde Oka Pemayun Tjokorda Sari Nindhia Tono PG, I Ketut TRI KOMALA SARI Tyas Pandieka Yoga Velia Chyntia Victoria Widyasanti, Ni Wayan Helpina Wijaya, Anak Agung Gede Oka Wira, Amar Wuriandari, Paula Bintang Yasa, Gede Sastra Darma Yeni Ratna Sari Yeyen Agustianingsi Yolla Noviolita Yoshihiro Hayashi YUNNY DAMAYANTI Yustisia, Anggia