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All Journal Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Microbiology Indonesia Media Kedokteran Hewan Jurnal Medik Veteriner Journal of Applied Veterinary Science and Technology Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences

Ida Bagus Oka Winaya

Faculty Of Veterinary Medicine, Udayana University

Author-ID : 336764

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Kejadian Balantidiosis pada Babi Landrace (A CASE STUDY OF BALANTIDIOSIS IN LANDRACE SWINE ) Ida Bagus Oka Winaya; I Ketut Berata; Ida Ayu Pasti Apsari
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of the study was to identify the incidence of balantidiosis in landrace pigs. A total of 60 pigswere examined at Faculty of veterinary medicine, Udayana University between January 2007 and January2008. Seven out of go pigs showed cahexia and diarrhoea . Macroscopic changes were observed, such as: thecolon was fully distended with gas and slight peritonitis,whereas microscopic examination revealed thepresence of Balantidium coli trophozoites and cysta within the intestinal mucosa. Additionally, enteritiskatarrhalis, slight hemorrhagis, erosin and pseudomembranous inflammation with lymphocytes andpolymorphonuclear cells were also noted.

BLOOD SMEAR EVALUATION OF BALI DUCKS SAMPLED FROM TRADITIONAL FARMING SYSTEMS IN BALI Iwan Haryono Utama; Sugiyarto -; Anak Agung Sagung Kendran; Ida Ayu Pasti Apsari; I Nyoman Suarsana; I Gusti Made Krisna Erawan; Anak Agung Ayu Mirah Adi; Ida Bagus Oka Winaya; Yoshihiro Hayashi
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Research to confirm the abnormalities of blood corpuscles in Bali ducks based on blood smear examination has been conducted. The research samples consisted of 105 ducks from various regencies in Bali. The blood smear examination was conducted by the method described in this literature and the data was collected and tabulated by means of the descriptive method. Erythrocyte abnormalities were: polychromasia (61.9%), anisocytosis (17.1%), poikilocytosis (21.0%). Abnormalities associated with leukocyte composition were: Lymphopenic (46.7%); Heterophylic (44.8%); Heteropenic (3.8%), also Thrombocyto penia (2.9%) was present in 105 ducks. In conclusion 61,9% ducks examined deal with regenerative anaemia. They also have some blood abnormalities such as basophylia, eosinophylia, heterophylia, heteropenia, lymphopenia, and monocytosis.

Gambaran Sitologi Cairan Peritoneal dan Sinovial Itik Bali (CYTOLOGIC FIGURES OF PERITONEAL AND SYNOVIAL FLUIDS IN BALI DUCKS) Iwan Harjono Utama; Sri Kayati Widyastuti; Anak Agung Ayu Mirah Adi; I Gusti Made Krisna Erawan; Ida Bagus Oka Winaya; Ida Bagus Komang Ardana; Achoiro Wati Rasid; Tyas Pandieka Yoga
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study aim was to observe cellular composition and morphology in peritoneal and synovial fluids ofbali ducks. This study used 50 ducks, which were composed of 21 males and 29 females aged 8 to 12 weeks.After being declared clinically healthy, the sample ducks were slaughtered and peritoneal fluid specimenfrom the abdominal cavity and the synovial fluid of joints tibiotarsal were collected. Then, in preparationfor the specimen, each of fluid was smeared and stained with Giemsa. Observations were made under amicroscope with a magnification of 1000 times and observations expressed in the average number of cellsper field of view observation. The results showed (duck population that most of the observed sample): inthe peritoneal fluid there was 36% eosinophils observed and 38% had heterophile cells with 0-3 cells perfield of magnification 1000x view. Similarly, 28% ducks had lymphocytes with 13-16 cells, and as much as28% of ducks had macrophage cells with 5- 8 cells in observation . In the synovial fluid, however, 64%sample ducks had monocytes, while 72% having lymphocytes with 1-18 cells each of view field withmagnification 1000x. In addition, in the synovial fluid, as much as 62% of ducks also observed had 1 to 3cells heterophiles and 60% ducks had eosinophils (polimorphonuclear cells) per view field observation.Macrophage cells that were found in the peritoneal fluid were mainly hyper granulated. It can be concludedthat the amount of mononuclear cells in both types of body fluids is likely more dominant compared to thenumber of polymorphonuclear cells.

Kadar Perasan Kunyit yang Efektif Memperbaiki Kerusakan Hati Mencit yang Dipicu Karbon Tetrachlorida (THE EFFICACY OF TURMERIC JUICE ON PATHOLOGICAL CHANGES OF THE MICE LIVER BY INDUCED CARBON TETRACHLORIDE) I Made Kardena; Ida Bagus Oka Winaya
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study on effect of various concentrations of turmeric juice on pathological changes mice liver hasbeen carried out. As many as 25 mice were used in this study and they were divided into 5 groups, i.e.: groupK-: 0.2 ml vegetable oil without CCl4; group K+: CCl4 without turmeric extract; group T1: CCl4 with 10%turmeric extract; group T2: CCl4 with 20% turmeric extract and group T3: CCl4 with 30% turmeric extract.All mice, except the K- group, were treated with 0.2 ml CCl4 orally for 7 days. On day-8 to day-29, the CCl4treated mice were given various concentrations of turmeric extract, i.e.: 10%, 20% and 30%; whereas, the KandK+ groups were given drinking water adlibitum. On day-30, all mice were sacrificed and their livertissues were collected and then processed to histopathological staining with haematoxylin-eosin. Theresult showed a clear evidence to suggest that turmeric extract can induce a better recovery compared tonon-treated mice. The improvement of pathological condition was characterized by reduction of pathologicalchanges found in the liver cells of mice, such as: hidrophic degeneration. fatty degeneration, and necrosis.More importantly, treatment with 30% of turmeric juice induced a better recovery than those with 10%and 20%.

Pelacakan Ekspresi Antigen Toxoplasma gondii secara Imuno(sito)histokimia (TRACKING EXPRESSION OF TOXOPLASMA GONDII ANTIGENS USING IMMUNO(CYTO)HISTOCHEMISTRY METHOD) Ida Ayu Pasti Apsari; Ida Bagus Oka Winaya; Tjokorda Sari Nindhia; Ida Bagus Ngurah Swacita
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The research objective want to track the expression of Toxoplasma gondii antigenes in the heart of the free-range chicken with immuno(cyto)histochemistry technique. In the present study were examined three methods to track antigen T.gondii, first method direct microscopic examination of the heart digests, the second method to detect antigen of T.gondii with immunocytochemistry technique of the chicken heart digests and the third immunohistochemistry examination of the heart free-range chicken. The number of material samples examined were 100 heart free-range chicken. Direct microscopic examination of the heart digests free-range chicken to track the bradyzoite form (inside cyst). Examination by immuno(cyto)histochemistry technique keep track T.gondii an antigen expression on cells and heart muscle tissue. The results showed that the direct microscopic examination on the heart tissue unable to detect cyst and antigen T.gondii. Immunohistochemical examination successfully detected the expression of antigenes T.gondii and was found 2% (2/100) positive. It can be concluded that T.gondii antigen expression in the heart of range chicken could be detected by immunohistochemistry.

Establishment and Characterization of Benzo(a)pyrene-Induced Skin Tumor in Rats Palagan Senopati Sewoyo; Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Marissa Divia Dayanti; Anak Agung Bagus Bramardipa; I Ketut Eli Supartika
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Quite a number of research on cancer therapy strongly require an animal model of cancer. One of the chemicals commonly used to induce cancer in animal models is benzo(a)pyrene due to its carcinogenic effects. This study aims were to describe the gross pathology of the tumor-induced by benzo(a)pyrene in an olive oil solution (w/v), identify the type of tumor histopathologically, and finally, determine the correlation between the duration of the rats experiencing tumor and it’s grade score. Tumor grade score is important to assess in order to determine tumor malignancy. This study consisted of 10 white rats (Rattus norvegicus) were given two treatments: a negative control treatment (K-) was injected with 0.1 mL of olive oil and a positive control treatment (K+) was injected with 0.1 mL of 0.3 % (w/v) benzo(a)pyrene in olive oil solution. Each treatment rats was kept in a cage and monitored regularly. When the tumors macroscopically appeared in the interscapular area and were observed until reached 4 cm in size, the rats were then sacrificed and necropsied. Tumors were observed for the gross pathology to examine the shape and color of them, then routinely processed for histopathological evaluation. The results showed that the tumors’ cells appeared to be round (1/5), irregular (2/5), and multilobular (2/5). Based on histopathological observation, the types of tumors observed were classical fibrosarcoma (2/5) and pleomorphic fibrosarcoma (3/5). There is a significant association between the duration of the rats experiencing tumors and the tumor grade. The longer the rats have tumors, the tumors tend to be more aggressive.

Pelacakan Secara Imunohistokimiawi Antigen Ekskretori-Sekretori pada Sapi Bali yang Terinfeksi Fasciola gigantica (IMMUNOHISTOCHEMICAL DETECTION OF EXCRET0RY-SECRETORY ANTIGENS IN BALI CATLLE INFECTED BY FASCIOLA GIGANTICA) Ida Bagus Oka Winaya; I Nyoman Mantik Astawa; I Made Damriyasa; Nyoman Sadra Dharmawan; I Ketut Berata
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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In order to study the distribution of excretory-secretory (ES) F. gigantica in liver tissue of infected balicattle a research was establisihed using monoclonal antibodies (mAbs) againts ES antigens. Immortalmouse myeloma cells were fused with the lymphocytes derived from the spleen of mice that immunizedwith ES antigen. The mAbs were tested for their specificity by using enzyme linked immunosorbent assay(ELISA). Five specific mAbs againts ES antigens were isolated and two mAbs were used for immunodetectionof ES antigens in liver tissue of bali cattle. Immunohistochemical ES antigens were not detected in paraffinembeded tissue of negative confirmed fasciolosis samples. ES antigens was detected in hepatocytes andcytoplasm of bile duct epithelims in the bali cattle that infected with fasciolosis in moderate intensity.Therfore indicated that mAbs produced in this study are applicable for detecting ES antigens in bali cattleinfected by F. gigantica.

Immunological Detection of Avian Influenza Virus in Infected Ducks by Monoclonal Antibodies Against AIV-H5N1 NYOMAN MANTIK ASTAWA; IDA BAGUS OKA WINAYA; LUH PUTU AGUSTINI; NINING HARTANINGSIH
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

In order to establish a detection method for avian influenza virus (AIV) infection in ducks, monoclonal antibodies (MAbs) against the virus were produced. The virus used for the production of the monoclonal antibodies was AIV-H5N1 of Indonesian origin. Immortal mouse myeloma were fused with the lymphocytes derived from the spleen of mice immunized with the virus. The MAbs were tested for their specificity by enzyme linked immunosorbent assay (ELISA) and western blotting using formaldehyde inactivated virus and normal allantoic fluid as a negative control. Twelve MAbs which were specific against AIV were isolated and 8 of them were used for detecting of AIV antigen in duck’s tissues. AIV antigen was detected in paraffin embedded tissues of AIV-infected ducks by immunohistochemistry using MAbs. AIV antigen was not detected in ducks, which were confirmed to be AIV negative. In the infected ducks, high intensity of AIV infection was detected in proventricle gland and small intestine. The AIV antigen with a lesser intensity was also detected in lungs, spleen, and bursa of Fabricius, but hardly detected in muscle, brain, and several other issues. This study shows a clear evidence that MAbs produced in this study are applicable for use in immunological detection of AIV in infected duck tissues.

Perubahan Histopatologi Hati Ayam Kampung yang Diberikan Jamu Daun Ashitaba dan Divaksin Avian Influenza I Gede Made Andy Pratama; I Wayan Sudira; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p11

Ashitaba plant (Angelica keiskei) is one of the original herbal plants from Japan which physically resembles celery leaves and can treat various diseases. This study aims to see the effect of giving ashitaba leaf herbal medicine on the histopathological picture of native chicken liver (Gallus domesticus) as seen from the proliferation of Kupffer cells in sinusoids vaccinated with Avian influenza (AI) vaccine. This study used a completely randomized design and used 25 native chickens. The study consisted of a treatment group, namely giving Ashitaba leaf herbal medicine (control), as well as giving Ashitaba leaf herbal medicine with doses of 50, 100, 200, and 400 mg / head / day respectively for 14 days by mixing it in drinking water until the volume was 100 ml. On the 21st day, all groups of chickens were vaccinated with the Avian Influenza vaccine. Liver from samples taken on day 42, to be made preparations and stained with Hematoxylin-Eosin staining. Sample under a microscope at 400x magnification. The variable observed was the proliferation of kupffer cells in the sinusoids with the histopathological change scoring scale. The research data were analyzed non-parametrically using the Kruskal-Wallis test. The results showed that giving the herb Ashitaba leaves with a dose range of 50 mg / head / day to 400 mg / head / day for 14 days, did not cause significant changes in the histopathology of native chickens when seen from the proliferation of Kupffer cells in the sinusoids that had been induced by avian influenza vaccine.

Perubahan Histopatologi Limpa Ayam Kampung yang Diberikan Jamu Daun Ashitaba dan Divaksin Avian Influenza Velia Chyntia Victoria; I Wayan Sudira; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p07

This study aims to determine the effect of giving ashitaba leaf herbal medicine as seen from the histopathological picture of native chicken spleen vaccinated against avian influenza disease vaccine. This research uses a complete random design. The treatment consisted of five treatment groups namely without ashitaba (control), with an ashitaba dose of 50; 100; 200, and 400 mg / head were dissolved with water until the volume is 100 ml and given treatment for 14 days. On the 21st day, all groups of native chickens were vaccinated against the avian influenza vaccine. On the 42nd day, native chickens were euthanasia by cervical dislocation, then the spleen was taken to see changes in the histopathological structure. Then it continued with Hematoxylin-Eosin (HE) staining and was tested under a microscope at 400x magnification. Variables that are seen from the proliferation of lymphoid follicles on white pulp by using a scaling scale for histopathological changes. The research data were analyzed by analysis non parametric Kruskal-Wallis. The results showed that there was a significant change in spleen histopathology between treatment P0 (control) and treatment given the herbal ashitaba leaf. Giving the herbal ashitaba leaf at a dose of 400 mg/head/day improves the body defense system of native chickens vaccinated with avian influenza.

Co-Authors Achoiro Wati Rasid Aida Lousie Tenden Rompis Anak Agung Ayu Mirah Adi Anak Agung Ayu Mirah Adi Anak Agung Bagus Bramardipa Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Anak Agung Sagung Kendran Aras, Fatmawati Astuti, Ni Made Widy Matalia Baiq Renny Kamaliani BERATA BERATA Bire, Ienoliski Rohi Brigietta Vincencia Simanihuruk Cahyani, Ni Kadek Devi Desak Wiga Puspita Dewi Dewa Odiec Purnawan Djara, Devita Vanessa Sukmawati DWI SURYANTO Dzikri Nurma'rifah Takariyanti Elyda . Fatmawati Aras Febrianty, Ni Made Dhea Guru, Yohana Cendyka Kartika Dewi Gusti Agung Ayu Putu Adriyati, Gusti Agung Ayu Gusti Ayu Yuniati Kencana Husain, Anugrah Fitria I Dewa Made Adhiwitana I Gede Made Andy Pratama I Gusti Agung Arta Putra I Gusti Agung Gede Putra Pemayun I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Narendra Putra1, I Kadek Parnayasa I Ketur Berata I Ketut Berata I Ketut Eli Supartika I Ketut Eli Supartika I Ketut Eli Supartika I Made Damriyasa I Made Kardena I Made Merdana I Made Merdana I Made Sukada i Nengah Wandia I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Arya Adikara I Wayan Gorda I Wayan Sudarmayasa I Wayan Sudira I Wayan Wirata I.K. Berata IB. KADE SUARDANA Ida Ayu Adhistania Pidada Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Sri Chandra Dewi Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Windia Adnyana Iwan Harjono Utama Iwan Haryono Utama Kadek Karang Agustina Komang Andika Purnama Komang, Nadia Eprillia Sary Darma Ni Kurnia, Ida Ayu Gintan Aristi Luh Made Sudimartini LUH PUTU AGUSTINI Luh Putu Ratna Suhita Lutviana, An'nisafitri M. Farhan Al Ma'arif Marissa Divia Dayanti Marissa Divia Dayanti Marson, Fransiska Gratia Sonita MAS DJOKO RUDYANTO Mia Monica Ni Kadek Eka Widiadnyani Ni Kadek Nining Laksmi Dewi Ni Komang Wijayani Ni Luh Aricahyani Ni Luh Eka Setiasih Ni Luh Putu Ratna Suhita Ni Nyoman Werdi Susari Ni Putu Trisna Asih NINING HARTANINGSIH Nur Baiti Palagan Senopati Sewoyo Palagan Senopati Sewoyo Pinem, Nuh Lasjuardi Purnama, Komang Andika Purnasari, Maria Elisabeth Putra, I Putu Werdikta Jayantika Putu Agus Trisna Kusuma Antara Putu Henrywaesa Sudipa Putu Intan Kusuma Wardani Rahmat Grahadi RAHMI MUSTABA Ryan Helmi Habibi Samsuri Samsuri Sartika, Nola Alfien Sartika, Nola Alfieni Sewoyo, Palagan Senopati Shafira Laili Aulia Sri Kayati Widyastuti Sugiyarto - Tampubolon, Yandri Putra Lumatas Theresia Ene Tjok Gde Oka Pemayun Tjokorda Sari Nindhia Tono PG, I Ketut TRI KOMALA SARI Tyas Pandieka Yoga Velia Chyntia Victoria Wijaya, Anak Agung Gede Oka Wira, Amar Yasa, Gede Sastra Darma Yeni Ratna Sari Yoshihiro Hayashi YUNNY DAMAYANTI Yustisia, Anggia

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