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<![CDATA[PERUBAHAN HISTOLOGIS DAN RESPONS IMUNITAS SAPI BALI YANG DIBERIKAN PAKAN CAMPURAN KONSENTRAT ]]>
<![CDATA[I ketut Berata]]>;
<![CDATA[Ida Bagus Oka Winaya]]>;
<![CDATA[I Made Kardena]]>
<![CDATA[ Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September ]]>
Publisher : <![CDATA[Universitas Syiah Kuala ]]>
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DOI: 10.21157/j.ked.hewan.v6i2.307
<![CDATA[Penelitian ini bertujuan mengetahui perubahan histologis dan respons kekebalan sapi bali yang diberikan pakan campuran konsentrat. Sebanyak 12 ekor sapi bali betina berumur 2 tahun, dibagi secara acak atas 3 kelompok perlakuan yaitu kelompok I diberi pakan rumput, kelompok II diberi pakan campuran 2 bagian rumput dan 1 bagian konsentrat, dan kelompok III diberi pakan campuran 1 bagian rumput dan 1 bagian konsentrat. Sebelum diberi perlakuan, dilakukan uji respons kekebalan seluler dengan teknik uji methylthiazol tetrazolium (MTT). Uji respons kekebalan dilakukan kembali pada bulan ke-3 dan sesaat sebelum dilakukan nekropsi. Pada bulan ke-10 dilakukan nekrosi terhadap 2 ekor sapi dari masing-masing kelompok perlakuan. Sisa sapi dari masing-masing kelompok perlakuan dilanjutkan diberi perlakuan untuk penelitian lanjutan. Sapi yang dinekropsi diambil jaringan pencernaan yaitu usus, untuk selanjutnya diproses dalam pembuatan sediaan histologis. Sediaan histologis diwarnai dengan hematoksilin eosin (HE). Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan struktur histologis usus antara ketiga kelompok perlakuan sedangkan respons kekebalan seluler tertinggi pada kelompok yang diberi pakan konsentrat yang lebih banyak.]]>
<![CDATA[Gambaran Histopatologi Pulmo Ayam Broiler yang Diberi Infusa Daun Dadap dan Mengalami Stres Pengangkutan ]]>
<![CDATA[Ryan Helmi Habibi]]>;
<![CDATA[Ida Bagus Oka Winaya]]>;
<![CDATA[I Made Merdana]]>
<![CDATA[ Buletin Veteriner Udayana Vol. 15 No. 5 October 2023 ]]>
Publisher : <![CDATA[The Faculty of Veterinary Medicine, Udayana University ]]>
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DOI: 10.24843/bulvet.2023.v15.i05.p02
<![CDATA[To get to the hands of consumers, it is necessary to transport broiler chickens from the warehouse to the marketing place. The transportation process often causes stress to broiler chickens, this is due to the long distance traveled when transportation is inadequate. This situation causes changes in organs, one of which is the lungs. Due to the potential of Dadap leaf as an antistress, this study aims to determine the effect of Dadap leaf infusion (Erythrina subumbrans) on pulmonary histopathology (Pulmonary ventilation) of broiler chickens experiencing transportation stress. A total of 30 male broiler chickens aged 4 weeks were taken as samples in this study. This study used a completely randomized design (CRD) with a factorial 3×2×5 pattern with three replications and the number of samples used were 30 broiler chickens which were divided into 5 treatment groups, namely negative control (P0), vitamin C 2 grams/L (P1), 1000 ppm Dadap leaf infusion (P2), 2000 ppm Dadap leaf infusion (P3) and 3000 ppm Dadap leaf infusion (P4). The data obtained were analyzed using the Kruskal-Wallis test. If there is a difference, it is continued with the Mann-Whitney test. performed using a scoring method on five visual fields, with a score of one meaning mild/focal, two to three meaning moderate/multifocal, more than three meaning severe/diffusion. The results showed that the mean of pulmonary histopathological damage with accurate variables was significantly different. The data obtained were the highest damage occurred in the P0 group (negative control) and the lowest damage occurred in the P3 group (3000 ppm dadap leaf infusion). The conclusion of this study is that 10% Dadap leaf infusion can reduce pulmonary damage and can be used as an antioxidant.]]>
<![CDATA[Patological Changes in Liver and Gall Bladder Of Bali Cattle Infected by Fasciolosis ]]>
<![CDATA[I Made Kardena]]>;
<![CDATA[Ida Bagus Oka Winaya]]>;
<![CDATA[Anak Agung Ayu Mirah Adi]]>;
<![CDATA[I Ketut Berata]]>;
<![CDATA[Ida Bagus Windia Adnyana]]>;
<![CDATA[I Made Sukada]]>;
<![CDATA[Kadek Karang Agustina]]>;
<![CDATA[Putu Agus Trisna Kusuma Antara]]>
<![CDATA[ Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017) ]]>
Publisher : <![CDATA[Institute for Research and Community Service, Udayana University ]]>
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DOI: 10.24843/JVAS.2017.v01.i01.p02
<![CDATA[Fascioliosis is a parasitic disease that infects ruminants and the disease is widely spread in the world. Fascioliosis caused by Fasciola hepatica and Fasciola gigantica that can cause macroscopic and microscopic lesions in the liver and gall bladder of bali cattle. Samples of bali cattle in Pesanggaran slaughter house that infected with fasciolosis were used in this study. The pathological macroscopic and microscopic changes of the liver and gall bladder were observed. The parasite found in the liver and gall bladder, thickening of bile duct mucous were observed on macroscopic examination. However, in microscopic observation found infiltration of inflammatory cells, fibrosis, necrosis, and degeneration of hepatocytes. In the gall bladder, necrosis was found in epithelial mucosal bile duct, infiltration of collagen fibers, inflammatory cells, hypertrophy and hyperplasia of the bladder epithelium were occurred.]]>
<![CDATA[Tracking Antigen Expression of Toxoplasma gondii in Mice by Immuno (Cyto) Histochemistry ]]>
<![CDATA[Ida Ayu Pasti Apsari]]>;
<![CDATA[Ida Bagus Oka Winaya]]>;
<![CDATA[Tjokorda Sari Nindhia]]>;
<![CDATA[Ida Bagus Ngurah Swacita]]>
<![CDATA[ Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017) ]]>
Publisher : <![CDATA[Institute for Research and Community Service, Udayana University ]]>
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DOI: 10.24843/JVAS.2017.v01.i01.p08
<![CDATA[Mouse has been an animal model that is very sensitive towards the Toxoplasma gondii infection. This research infects 30 mice with free-range chicken heart inoculate and the detection of T. gondiiby immunohistochemistry on the infected mice. The purpose of this study is to track T.gondii antigen expression by immune(cyto)histochemistry in mice. Observation over mice was conducted over 10 weeks. After 10 weeks, the mice are examined for its peritoneal fluid by microscopic and immunohistochemistry examination. Continued by the examination of internal organs to track the expression of T.gondii antigens by immunohistochemistry. The study result showed microscopic examinations on peritoneal fluid did not found any cyst or tachyzoites form of T.gondii. Examination of peritoneal fluid by immunohistochemistry was found to detect expressions of T.gondii antigen. Examinations of the mice’s internal organs detected expressions of T.gondii antigen only in the brain by immunohistochemistry examinations.]]>
<![CDATA[Gambaran Histopatologi Jantung Tikus Putih Dengan Fibrosarcoma Pasca Viroterapi Virus Newcastle Disease Isolat Gianyar-1/AK/2014 ]]>
<![CDATA[Brigietta Vincencia Simanihuruk]]>;
<![CDATA[Ida Bagus Oka Winaya]]>;
<![CDATA[Anak Agung Ayu Mirah Adi]]>
<![CDATA[ Buletin Veteriner Udayana Vol. 15 No. 6 December 2023 ]]>
Publisher : <![CDATA[The Faculty of Veterinary Medicine, Udayana University ]]>
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DOI: 10.24843/bulvet.2023.v15.i06.p31
<![CDATA[Virotherapy is a type of cancer therapy that utilizes the properties of viruses to destroy cancer cells. This therapy uses an oncolytic virus can destroys cancer cells. In the study, the oncolytic virus used was the virusNewcastle Disease. Virus Newcastle Disease known to be non-pathogenic in mammals so that this virus can be used as a cancer treatment agent. This study aims to determine the histopathological description of Newcastle Disease virus virotherapy on the cardiac histology of white rats with fibrosarcoma. Mice were grouped into two treatment groups, namely P0 (treatment 0) with fibrosarcoma treated with PBS and P1 (treatment 1) with fibrosarcomas treated with a virus Newcastle Disease at a dose of 0,5 ml/28 HA units. Histopathological view of the heart of P1 white rats shows hemorrhagic lesions, congestion, and edema. The results of the microscopic examination were then quantified by scoring and analyzed using non-parametric tests. The results of the observation showed that Newcastle Disease virotherapy did not give significant changes in hemorrhagic lesions, congestion and edema (P>0,05).]]>
<![CDATA[Gambaran Histopatologi Hepar Tikus Putih Penderita Fibrosarkoma Pasca Viroterapi Newcastle Disease Isolat Virulen ]]>
<![CDATA[Ni Luh Aricahyani]]>;
<![CDATA[Anak Agung Ayu Mirah Adi]]>;
<![CDATA[Ida Bagus Oka Winaya]]>
<![CDATA[ Buletin Veteriner Udayana Vol. 15 No. 6 December 2023 ]]>
Publisher : <![CDATA[The Faculty of Veterinary Medicine, Udayana University ]]>
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DOI: 10.24843/bulvet.2023.v15.i06.p06
<![CDATA[Newcastle Disease virus as the cause of tetelo disease in Indonesia or avian paramyxovirus 1 (APMV-1) is a type of oncolytic virus used as a virotherapy agent, due to its ability to lyse cancer cells in mammals including fibrosarcoma. As is known, the liver is an organ that functions in the detoxification of toxic and foreign materials that enter the body. The purpose of this study was to determine the side effects of the virulent isolate Newcastle Disease virus Gianyar-1/AK/2014 as a virotherapy agent on the histopathological features of the liver of white rats with fibrosarcoma. This study used 6 rats model of fibrosarcoma induced by carcinogens and then divided into two treatment groups consisting of three rats as replicates. Treatment 0 (P0) was injected with phosphate buffer saline (PBS) and Treatment 1 (P1) was injected with Newcastle Disease virus isolate Gianyar virulent-1/AK/2014 at a dose of 0.5 mL / 28 HA units. The histopathological features observed at 400X magnification were in the form of degenerative lesions and necrosis of hepatocyte cells and were then given a score according to their severity. The results of the Mann-Whitney U statistical test showed that there was no significant difference in the score of degenerative hepatocyte lesions between the two treatments (P>0.05). Meanwhile, necrotic lesions at P1 were lower when compared to necrotic lesions at treatment P0 (P<0.05). Based on these results it can be concluded that this virotherapy does not damage liver cells and intratumoral injection of the virus causes a better histopathological overview of the liver compared to those not given virotherapy. However, further research is still needed regarding the effect of giving the virus to liver cells in order to see the effectiveness of fibrosarcoma cancer treatment using the Newcastle Disease virus.]]>
<![CDATA[Efek Viroterapi Virus Newcastle Disease Isolat Gianyar-1/Ak/2014 pada Histopatologi Limpa Tikus Putih Model Fibrosarkoma ]]>
<![CDATA[Shafira Laili Aulia]]>;
<![CDATA[Ida Bagus Oka Winaya]]>;
<![CDATA[Anak Agung Ayu Mirah Adi]]>
<![CDATA[ Buletin Veteriner Udayana Vol. 15 No. 6 December 2023 ]]>
Publisher : <![CDATA[The Faculty of Veterinary Medicine, Udayana University ]]>
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DOI: 10.24843/bulvet.2023.v15.i06.p22
<![CDATA[Fibrosarcoma is cancer that originates from fibroblast cells that grow uncontrollably and indefinitely. Surgery, chemotherapy, and radiotherapy are commonly used anticancer therapies, but still have a negative effect on normal body cells, so alternative therapies are carried out using replication of oncolytic viruses, namely Newcastle disease virus. The purpose of this study was to determine the damage or negative effects on the spleen organ after virotherapy using Newcastle disease virus by observing the histopathological picture. This study used 12 rats that had been induced benzo(a)pyrene, after macroscopically observed cancer with a diameter of 3-4 mm, 6 tails with almost uniform cancer size. Grouped into two treatments, namely the control group (P0) injected with Phosphate Buffer Saline (PBS) amounting to 0.5 mL and the treatment group (P1) injected with Newcastle disease virus at a dose of 0.5 mL/28 HA units. The variables observed were congestion lesions, hemorrhage, and necrosis. The results of histopathological observations from both groups showed no specific damage to the spleen organ, but lymphocyte proliferation was observed. Based on these results, it can be concluded that virotherapy with Newcastle disease virus isolate Gianyar-1/AK/2014 has no effect on the histopathology of the spleen of white rats with fibrosarcoma.]]>
<![CDATA[Gambaran Histopatologi Ginjal Tikus Putih Penderita Fibrosarcoma Pasca Viroterapi Virus Newcastle Disease ]]>
<![CDATA[Ni Komang Wijayani]]>;
<![CDATA[Anak Agung Ayu Mirah Adi]]>;
<![CDATA[Ida Bagus Oka Winaya]]>
<![CDATA[ Buletin Veteriner Udayana Vol. 15 No. 6 December 2023 ]]>
Publisher : <![CDATA[The Faculty of Veterinary Medicine, Udayana University ]]>
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DOI: 10.24843/bulvet.2023.v15.i06.p10
<![CDATA[Newcastle disease virus is an oncolytic virus that has great potential as a cancer virotherapy because it can selectively infect and replicate in tumor cells. Virotherapy with ND virus can also stimulate the immune system, and lyse tumor cells with limited or non-pathogenic pathogenicity against mammals. The purpose of this study was to determine the side effects of virotherapy of the Newcastle disease virus, the virulent isolate Gianyar-1/AK/2014 on the kidneys by observing its histopathological picture. This study used a model of six fibrosarcoma rats which were randomly divided into two treatment groups where each treatment consisted of 3 rats as replicates. The non-therapeutic treatment (P0) was injected with Phosphate Buffer Saline (PBS) and the therapeutic treatment (P1) was injected with the Newcastle Disease virus isolate Gianyar-1/AK/2014 virulent at a dose of 0.5 ml 28 units of HA. Renal histopathology at P0 and P1 showed necrotic, hemorrhagic, and congestion lesions. The severity of the kidney lesions was scored and then the data obtained were analyzed using the non-parametric Mann-Whitney U test. The results of this study found that necrotic lesions were milder in P1 rats compared to P0. As for hemorrhage and congestion, there was no significant difference between treated and untreated rats. Based on the results of this study, it can be interpreted that virotherapy of the Newcastle disease virus virulent isolate Gianyar-1/AK/2014 reduced necrotic lesions (p<0.05), while for hemorrhagic and congestive lesions did not increase the severity of the lesions (p>0.05) so that This shows that virotherapy with Newcastle disease virulent isolate Gianyar-1/ AK/ 2014 does not cause any adverse effects on the kidneys.]]>
<![CDATA[Mammary Tumors in Dogs, Recent Perspectives and Antiangiogenesis as a Therapeutic Strategy: Literature Study ]]>
<![CDATA[Sewoyo, Palagan Senopati]]>;
<![CDATA[Adi, Anak Agung Ayu Mirah]]>;
<![CDATA[Winaya, Ida Bagus Oka]]>;
<![CDATA[Wirata, I Wayan]]>
<![CDATA[ Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
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DOI: 10.20473/jmv.vol6.iss2.2023.271-287
<![CDATA[A particular type of tumor that is frequently detected in female dogs who are sexually active is a mammary tumor. Neoplasia results from DNA-based alterations in cell cycle regulating genes. The mammary gland is prone to the formation of tumors due to its dynamic structure. The development of this tumor is supported by numerous variables. It has been recently discovered that there is substantial evidence linking the BRCA2 gene to the process of cancer. Standard examination techniques, such as fine needle aspiration, histopathology, and immunohistochemistry, are used along with ancillary tests to determine the tumor type and degree of malignancy. The primary treatment option for malignant tumors is surgical resection followed by adjuvant chemotherapy; benign tumors necessitate surgical resection as well. Adjuvant therapy options include hormone therapy and non-steroidal anti-inflammatory medications. Tumor tissue undergoes angiogenesis as it grows and develops to accommodate the abundant supply of nutrients. Therefore, angiogenesis-inhibiting therapies can be utilized to halt the growth of tumor cells. A number of antiangiogenic medications are now being studied in clinical settings on humans, and several more are undergoing trials on animals. In addition to pharmaceuticals, viruses may be used as a therapeutic to block tumor angiogenesis.]]>
<![CDATA[Sebaceous Adenoma in a Geriatric Poodle Dog: A Case Report ]]>
<![CDATA[Winaya, Ida Bagus Oka]]>;
<![CDATA[Adi, Anak Agung Ayu Mirah]]>;
<![CDATA[Sudimartini, Luh Made]]>;
<![CDATA[Merdana, I Made]]>;
<![CDATA[Sudipa, Putu Henrywaesa]]>;
<![CDATA[Pemayun, I Gusti Agung Gde Putra]]>;
<![CDATA[Sewoyo, Palagan Senopati]]>
<![CDATA[ Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
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DOI: 10.20473/jmv.vol7.iss2.2024.413-419
<![CDATA[A 14-year-old black male Poodle was brought to the Animal Teaching Hospital, Udayana University by its owner with clinical signs of frequent licking of its left front paw. Upon examination, red bumps were observed on the left front leg, accompanied by small, round black spots scattered on the dorsal side of the body. Additionally, black nodules were present on the lower eyelids and hind limbs. Surgical intervention was undertaken to excise the tumor mass, with the animal under anesthesia induced by ketamine at 5 mg/kg BW intravenously. The reddish nodule was excised by performing an elliptical incision at the base of tumor. Postoperatively, the animal received an antibacterial injection comprising ceftriaxone and tazobactam at 25 mg/kg BW intramuscularly and antiseptic wound dressing for supportive care. Microscopic examination revealed neoplastic cells arranged into lobules of varying sizes and shapes within the tumor mass. These lobules consisted of differentiated sebocytes and basaloid cells. At the periphery of the neoplastic lobules, the basaloid cells displayed several layers and exhibited invasion with moderate anisocytosis. The mitotic index was no more than ten cells in one field of view. Based on these histopathological features, the tumor was confirmed to be a sebaceous adenoma. After a 10-month follow-up period, there were no signs of tumor recurrence observed.]]>
