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All Journal AKULTURASI (JURNAL ILMIAH AGROBISNIS PERIKANAN) AQUATIC SCIENCE & MANAGEMENT e-Journal BUDIDAYA PERAIRAN JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX JURNAL PERIKANAN DAN KELAUTAN TROPIS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Jurnal Akuatika Indonesia Media Akuakultur
Henneke Pangkey
Sam Ratulangi University, Manado
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering

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Peningkatan kepadatan Alona sp. pada jerami padi yang difermentasi Utami, Tiara; Pangkey, Henneke; Lantu, Sartje; Lumenta, Cyska; Salindeho, Indra R. N.; Kemer, Kurniati
e-Journal BUDIDAYA PERAIRAN Vol 10, No 1 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.1.2022.35499

Abstract

The objective of this study was to determine the difference in density of Alona sp in fermented rice straw and unfermented rice straw media. This research was conducted at the Aquaculture Technology Laboratory and the Fish Health, Environment and Toxicology Laboratory, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University, Manado, from March to June 2021.The experiment was based on four treatments, namely fermented rice straw media with Effective Microorganisms-4 (EM-4) solution, unfermented rice straw media, horse manure media and water media. The experimental design used was a completely randomized design (CRD) with 4 treatments, each treatment was repeated 3 times, so there were 12 experimental units. The results obtained are the density of Alona sp. cultured on fermented rice straw media were not significantly different from those cultured on unfermented rice straw media but significantly different from horse manure and water media. There was no significant difference between fermented straw media and unfermented straw media on the increase in density of Alona sp., apparently caused by the fermentation time of EM-4 solution of rice straw which was not long enough.
Kultur pakan alami Alona sp. dalam media daun eceng gondok yang difermentasi dengan larutan Effective Microorganisme-4 Sumah, Maria; Solang, Jhonly; Pangkey, Henneke; Mokolensang, Jeffrie F.; Lumenta, Cyska; Sinjal, Hengky Sinjal; Rumampuk, Natalie D. C.
e-Journal BUDIDAYA PERAIRAN Vol 9, No 2 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.9.2.2021.34826

Abstract

The purpose of this study was to examine the density of life feed Alona sp. on the media of water hyacinth leaf fermented with effective microorganism-4 (EM-4) solution. The method used was an experimental, using one-way ANOVA, with four treatments, namely fermented water hyacinth leaf media, unfermented water hyacinth leaf media, horse manure media, and water, each with three replications. The parameter observed was the density of Alona sp. The result showed that the density of Alona sp. cultured using fermented water hyacinth leaf media was significantly different from those cultured on unfermented water hyacinth leaf media.  It had also been found that the density of Alona sp. high in horse manure media. However, it is recommended to use fermented water hyacinth leaf media, because it is more hygienic. In addition, the availability of horse manure is increasingly rare
Pertumbuhan dan kelangsungan hidup ikan nila Oreochromis niloticus yang dikultur pada sistem akuaponik dengan kepadatan biofilter kangkung yang berbeda Gerung, Pramulya R.A; Mudeng, Joppy D.; Salindeho, Indra R.N.; Longdong, Sammy N.J.; Pangkey, Henneke; Rumengan, Inneke F.M.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.38562

Abstract

This research was conducted to determine the optimal density of kale plants as biofilter in an aquaponic system that can trigger the highest growth and survival of tilapia. The experiment was designed in a completely randomized design (CRD) with 3 treatments and 3 replications so that there were 9 experimental units. The factor tested was the different density of kale plants as biofilter in the aquaponics system, where treatment A: 6 kale plants/0,24m2, B: 12 kale plants/0,24m2, and  K: without kale plants (as control). This study was conducted for 28 days.  Collected data  included weight, length and number of fish, which were then converted to  absolute growth rate (weight and length) , daily growth rate, survival rate and kale plant growth. Data  were analyzed using ANOVA  and then Least Significance Differences (LSD) for  further analysis.   The results  showed that the different density of  kale plants had no significant effect on absolute growth rate (weight and  length)  and daily growth rate, but had a significant effect on the survival rate of fish. The LSD results  showed that density of 6 kale plants/0,24m2 (A) were not significantly different from that of 12 individuals kale plants/0,24m2 (B), but both treatments were significantly different from the control (K).  The range of ammonia  was between 0.29 – 0.91 mg/l, nitrite 0.55 – 1.06 mg/l, and nitrate 10 - 47 mg/l.
Pertumbuhan benih ikan gabus (Channa striata) pada media pemeliharaan akuaponik menggunakan pakan komersil dengan komposisi protein berbeda Niti, Eukharistian; Mudeng, Joppy D.; Ngangi, Edwin L. A.; Pangkey, Henneke; Monijung, Revol D.; Longdong, Sammy N.J.
e-Journal BUDIDAYA PERAIRAN Vol. 12 No. 1 (2024): Januari - Juni
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v12i1.53959

Abstract

This research aimed to determine the growth of snakehead fries t reared in aquaponic media and fed commercial fish food. The treatments consisted of treatment A with a protein content of 40%, B with a protein content of 35%, and C with a protein content of 30%, each with three replications. The research was conducted for 32 days. The research found the absolute growth of treatment a was 1.34 g, treatment b was 0.775 g, and treatment C was 0.605 g. The daily growth rate for treatment a was 4.18%, treatment b 2.42%, and treatment C 1.89%. Linear regression analysis between protein content in food and growth weight of snakehead fish fry had a strong relationship to both absolute growth and specific growth rate. The higher the protein content, the higher the weight growth of snakehead fry. Keywords: snakehead fish, aquaponic, commercial food, protein
Penggunaan tepung daun kelor (Moringa oleifera) berbeda komposisi dalam formulasi pakan bagi pertumbuhan ikan nila (Oreochromis niloticus) Pamula, Doni F.; Lumenta, Cyska; Monijung, Revol D.; Mokolensang, Jeffrie F.; Pangkey, Henneke; Sambali, Hariyani
e-Journal BUDIDAYA PERAIRAN Vol. 12 No. 2 (2024): Juli - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v12i2.57387

Abstract

Several previous studies regarding the use of Moringa leaf flour on fish had been carried out fish consumption including catfish, tilapia, and gourami. The objectives of this research were to determine the effect of substitution of Moringa leaf flour at different doses in feed formulation on the relative growth of tilapia and determine which treatment had the highest feed efficiency value. The results showed relative weight growth value for tilapia were obtained in treatment C (83.82%),  daily growth (C) 2.03%, food conversion ratio (FCR) 1.29, and Feed Efficiency 77.01%. Keywords: relative growth, daily growth, feed conversion ratio, feed efficiency value.
Studi kandungan senyawa tributiltin dalam air pada area budidaya di Selat Lembeh Bitung Simanjorang, Daniel; Undap, Suzanne L.; Tumembouw, Sipriana S.; Pangemanan, Pankie N.L; Pangkey, Henneke; Mudeng, Joppy D.
e-Journal BUDIDAYA PERAIRAN Vol. 13 No. 1 (2025): Jan - Juni
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v13i1.59059

Abstract

Tributiltin (TBT) is an organotin compound that is still widely used as a toxic biocidal additive in antifouling paints. These compounds are classified as pollutants that are very difficult to decompose in waters. The test of the concentration of TBT in seawater was carried out at the WLN Laboratory (Water Laboratory Nusantara) Manado. Nitrate, nitrite and ammonia concentration tests were carried out at the Laboratory of the Manado Industrial Service and Standardization Center (BSPJI). Measurement of the physical and chemical parameters of seawater used: Temperature, Brightness, pH, Dissolved Oxygen (DO), Salinity and Turbidity in situ. Analysis of the concentration of Tributiltin (TBT) compounds in seawater in the waters of the Lembeh Strait in the cultivation areas of Point 1 (Tanjung Rarandam), Point 2 (Kalapa Dua), Point 3 (Papusungan) in the Lembeh Strait showed a concentration of <0.01 μg/L, which is still in the safe range below the standard limit of seawater quality for biota (0.01 μg/L). Measuring water quality in the Lembeh Strait aquaculture area, the DO value ranges from 7.83-8.34 ppm. Salinity values ranged from 30.99-31.24 ppt. The pH value ranges from 9.37-9.42. The temperature value ranges from 27.87-27.69 °C. The brightness value ranges from 3.12 m-3.39 m. The turbidity value ranged from 0.63-1.22. The nitrate value of 1.98-2.19 mg/L, far exceeding the limit set (0.06 mg/L) for marine life, indicates a potential risk to the ecosystem. The concentration of Nitrite in the waters is still in accordance with the general water standards, ranging from <0.005 mg/L to 0.02 mg/L. The concentration of Ammonia is below the water quality standard threshold at all points of 0.3 mg/L, which is still safe for marine life and aquaculture activities in these waters in accordance with the seawater quality standards in Government Regulation No. 22 of 2021 Attachment VIII concerning the Implementation of Environmental Protection and Management. Keywords: TBT Compounds, Concentration, Water Quality, Quality Standards, Straits
Induction Time And Sedative (Recovery) Time In Closed System Transportation Of Tawes Fish (Barbonymus gonionotus) Anesthetized With Clove Oil At Different Temperatures Saragih, Debby Dyanessa; Ngangi, Edwin L.A.; Pangkey, Henneke; Sumilat, Deiske Adeliene; Salindeho, Indra R.N.; Kreckhoff, Reni Lusia; Kusen, Diane Joula
Jurnal Ilmiah Platax Vol. 13 No. 1 (2025): ISSUE JANUARY-JUNE 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i1.60961

Abstract

The conservation of tawes fish (Barbonymus gonionotus) is carried out through aquaculture and restocking, where seed transportation is a crucial factor affecting survival rates. Mishandling during transport can increase stress, metabolism, and mortality risk. Reducing stress during transport can be achieved by using natural anesthetic agents such as clove oil (Eugenia aromatica) and low-temperature regulation. This study aims to analyze the effects of clove oil dosage and temperature on the induction time and recovery of tawes fish seeds. The experiment was designed using a completely randomized design (CRD) with two factors: clove oil dosage (0 ppm, 5 ppm, 10 ppm) and temperature (16–19°C and 24–27°C), with 18 experimental units (3 replications). Data were analyzed using ANOVA (JMP-SAS). The results showed that combining clove oil and temperature significantly affected induction and recovery times. The 10 ppm clove oil treatment at 16–19°C resulted in the fastest induction time, while the 0 ppm treatment at 24–27°C led to the fastest recovery. The interaction between these factors demonstrated that clove oil significantly influenced the fish's response to temperature changes. Keywords: tawes fish seed, transportation, temperature, clove oil, induction, sedative.   Abstrak   Pelestarian ikan tawes (Barbonymus gonionotus) dilakukan melalui budidaya dan restocking, di mana transportasi benih menjadi faktor krusial yang memengaruhi tingkat kelangsungan hidupnya. Kesalahan dalam penanganan dapat meningkatkan stres, metabolisme, dan risiko kematian benih. Upaya menekan stres selama transportasi dilakukan dengan penggunaan agen pembius alami, seperti minyak cengkih (Eugenia aromatica) dan pengaturan suhu rendah.Penelitian ini bertujuan menganalisis pengaruh dan dosis minyak cengkih serta suhu terhadap waktu induksi dan pemulihan (recovery) benih ikan tawes. Percobaan menggunakan rancangan acak lengkap (RAL) dengan dua faktor: dosis minyak cengkih (0 ppm, 5 ppm, 10 ppm) dan suhu (16–19°C dan 24–27°C), dengan 18 unit percobaan (3 ulangan). Data dianalisis menggunakan ANOVA (JMP-SAS).Hasil penelitian menunjukkan bahwa kombinasi minyak cengkih dan suhu berpengaruh signifikan terhadap waktu induksi dan pemulihan benih. Perlakuan 10 ppm minyak cengkih pada suhu 16–19°C menghasilkan waktu induksi tercepat, sedangkan tanpa minyak cengkih pada suhu 24–27°C mempercepat pemulihan. Interaksi kedua faktor ini menunjukkan bahwa minyak cengkih secara nyata mempengaruhi respons ikan terhadap perubahan suhu. Kata kunci: benih tawes, transportasi, suhu, minyak cengkih, induksi, sedatif.
Optimisation Of Papain Enzyme Use As An Effort To Increase The Hatching Degree Of Mutiara Catfish (Clarias gariepinus) Eggs Simangunsong, Martua Pinondang; Sambali, M.Sc, Hariyani; Pangkey, Henneke; Mokolensang, Jeffrie Fredrik; Pangemanan, Novie Pankie Lukas; Sumilat, Deiske Adeliene
Jurnal Ilmiah Platax Vol. 13 No. 1 (2025): ISSUE JANUARY-JUNE 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i1.61097

Abstract

This study aims to analyze the effect of commercial papain enzyme with different concentrations and duration of soaking on increasing the degree of hatching of pearl catfish (Clarias gariepinus) eggs. The study used a Randomised Group Design (RAK) with variations in papain enzyme concentration (0, 2, 4, 6, and 8 ppm) and soaking duration (5, 10, and 15 minutes). The results showed that papain enzyme concentration and soaking duration significantly affected the degree of hatching (p < 0,001). The optimal concentration of papain enzyme was 6 ppm with a soaking duration of 10 minutes, resulting in the highest hatching rate of 70,52%±2,92. The lowest egg adhesion was produced at a concentration of 8 ppm with a duration of 15 minutes (32%±4,00), while the highest degree of fertilization was directed at a concentration of 6 ppm with a duration of 10 minutes (79%±3,61). The measurement results of water quality parameters are: temperature 25,6-26,4°C, pH 7,12-7,47, and dissolved oxygen 4,06-4,41 ppm. These results indicate that the papain enzyme effectively reduces egg adhesion and increases the hatching rate of pearl catfish eggs.  Keywords: mutiara catfish; papain enzyme; hatching rate; concentration; immersion duration.   Abstrak Penelitian ini bertujuan untuk menganalisis pengaruh enzim papain komersial dengan konsentrasi dan durasi perendaman yang berbeda terhadap peningkatan derajat penetasan telur ikan lele mutiara (Clarias gariepinus). Penelitian menggunakan Rancangan Acak Kelompok (RAK) dengan variasi konsentrasi enzim papain (0, 2, 4, 6 dan 8 ppm) dan durasi perendaman (5, 10 dan 15 menit). Hasil penelitian menunjukkan bahwa konsentrasi enzim papain dan durasi perendaman berpengaruh signifikan (p <0,001) terhadap derajat penetasan. Konsentrasi optimal enzim papain adalah 6 ppm dengan durasi perendaman 10 menit, menghasilkan derajat penetasan tertinggi sebesar 70,52%±2,92. Daya rekat telur terendah dihasilkan pada konsentrasi 8 ppm dengan durasi 15 menit (32%±4,00), sedangkan derajat pembuahan tertinggi dihasilkan pada konsentrasi 6 ppm dengan durasi 10 menit (79%±3,61). Hasil pengukuran parameter kualitas air yaitu: suhu 25,6–26,4°C, pH 7,12–7,47 dan oksigen terlarut 4,06–4,41 ppm. Hasil ini menunjukkan bahwa enzim papain efektif mengurangi daya rekat telur dan meningkatkan derajat penetasan telur ikan lele mutiara.  Kata kunci: ikan lele mutiara; enzim papain; derajat penetasan; konsentrasi; durasi perendaman
Pengaruh Minyak Cengkih Terhadap Glukosa Darah dan Sintasan Ikan Tawes (Barbonymus gonionotus) pada Transportasi Sistem Tertutup Saragih, Debby Dyanessa; Ngangi, Edwin Leonardo Apolonio; Pangkey, Henneke; Kusuma, Ni Putu Dian; Sumilat, Deiske Adeliene; Salindeho, Indra R. N.; Kreckhoff, Reni Lucia; Joula Kusen, Diane
Akuatika Indonesia Vol 10, No 2 (2025): Jurnal Akuatika Indonesia (JAkI)
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan Universitas Padjadjaran, Grha. Kandaga (P

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jaki.v10i2.62511

Abstract

Pengangkutan benih ikan sering kali menyebabkan stres fisiologis yang berdampak negatif terhadap kesehatan dan kelangsungan hidup ikan, terutama akibat perubahan lingkungan seperti suhu dan perlakuan anestetik. Penelitian ini dilakukan untuk mengkaji pengaruh dosis minyak cengkeh dan suhu sebagai anestetik terhadap glukosa darah dan sintasan benih ikan Tawes. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) Faktorial, dengan dua faktor yakni faktor dosis minyak cengkih (A) dengan 3 taraf yaitu A1 (0 ppm), A2 (5 ppm), dan A3 (10 ppm), dan faktor suhu (B) terdiri dari 2 taraf yaitu B1 (16-19 °C) dan B2 sebagai kontrol (24-27 °C), dengan tiga kali ulangan. Penelitian ini terdiri atas 18 unit percobaan, di mana setiap unit diisi dengan 50 ekor benih ikan Tawes sehingga total ikan yang digunakan sebanyak 900 ekor. Benih dimasukkan ke dalam kantong berisi 5 L air (1/3 dari volume kantong), ditambahkan oksigen sebanyak 2/3 volume kantong. Selanjutnya dilakukan uji transportasi selama 12 jam, setelah proses pengangkutan, ikan dipelihara di akuarium berukuran 60 x 40 x 40 cm, dengan volume 96 L. Data dianalisis menggunakan analisis sidik ragam (ANOVA) pada program statistik JMP Pro 16 (SAS 9.4). Perlakuan terbaik ditemui pada perlakuan dosis minyak cengkih 10 ppm + suhu 24-27°C dengan kadar glukosa darah setelah transportasi dan setelah pemeliharaan masing-masing sebesar 119,9±6,80 mg/dL dan 86,56±5,01 mg/dL. Sintasan setelah transportasi dan setelah pemeliharaan masing-masing sebesar 98,7±1,16 % dan 96,7±1,16 %. Interaksi faktor minyak cengkih dan suhu menunjukkan bahwa faktor minyak cengkih tidak memberikan pengaruh secara nyata terhadap glukosa darah dan sintasan.
KARAKTERISTIK MORFOMETRIK DAN POLA PERTUMBUHAN IKAN NILA (Oreochromis niloticus) YANG DIBERI PAKAN MIKROKAPSUL INKLUSI BERBEDA Fintarji, Rian; Pangkey, Henneke; Mokolensang, Jeffrie Fredrik; Sambali, Hariyani; Kalesaran, Ockstan Jurike; Pangemanan, Novie Pankie Lukas; Sumilat, Deiske Adeliene
Media Akuakultur Vol 20, No 1 (2025): Juni 2025
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.20.1.2025.35-44

Abstract

Produksi perikanan budidaya dapat ditingkatkan melalui penyediaan pakan yang sesuai dengan kebutuhan ikan, mencakup aspek jenis, ukuran, maupun jumlahnya. Pakan mikrokapsul adalah jenis pakan buatan berukuran sangat kecil (mikron) yang dirancang khusus untuk memenuhi kebutuhan nutrisi ikan pada tahap larva dan benih.Tujuan penelitian ini adalah untuk menganalisis karakteristik morfometrik dan pola pertumbuhan ikan yang diberi pakan mikrokapsul inklusi berbeda. Penelitian ini menggunakan 1.080 ekor benih ikan nila merah berukuran 1-3 cm pada wadah akuarium. Akuarium yang digunakan berukuran 60x40x40 cm yang kemudian diisi air sebanyak 72 L dan ditebar sebanyak 1 ekor L-1. Penelitian ini didesain dengan menggunakan rancangan acak lengkap dengan lima perlakuan dengan masing-masing tiga ulangan. Perlakuan satu yaitu pemberian pakan komersil (kontrol), perlakuan dua yaitu pemberian pakan mikrokapsul tepung Tubifex sp., perlakuan tiga yaitu pemberian pakan mikrokapsul tepung Maggot, perlakuan empat yaitu pemberian pakan mikrokapsul tepung Spirulina sp., dan perlakuan lima yaitu pemberian pakan mikrokapsul tepung Chlorella sp.. Hasil penelitian menunjukkan bahwa karakteristik morfometrik ikan nila berbeda pada setiap perlakuan. Hasil uji regresi linear sederhana terhadap parameter pola pertumbuhan ikan nila pada semua perlakuan bersifat alometrik negatif, yakni pertumbuhan panjang lebih cepat daripada pertumbuhan beratnya. Hasil pengukuran kualitas air pada semua perlakuan menunjukkan hasil yang sesuai dengan baku mutu untuk pemeliharaan ikan nila dengan kisaran nilai suhu berkisar 25,1-29,6 o C, oksigen terlarut berkisar 5,1-6,8 mg L-1, pH berkisar 7,42-8,14, dan amonia berkisar 0,01-0,17 mg L-1.Aquaculture production can be increased by providing feed that is appropriate to the need of fish, both in terms of type, size, and quantity. Microcapsule feed is a type of artificial feed with very small sizes (microns) specifically designed to fulfil the nutritional need at the larval and fry fish stages. This study aimed to analyze the morphometric characteristics and growth patterns of fed microcapsules with different inclusions. The study was conducted using 1.080 tilapia with size of 1-3 cm in aquariums. The aquarium used was 60x40x40 cm and was then filled with 72 L of water with a stocking density of 1 fish L-1. This study used a completely randomized design with five treatments and three replications each. Treatment one is the provision of commercial feed (control), treatment two is the provision of microcapsule feed with Tubifex sp flour, treatment three is the provision of microcapsule feed with Maggot flour, treatment four the provision of microcapsule feed with Spirulina sp flour, and treatment five is the provision of microcapsule feed with Chlorella sp flour. The results of the study showed that the morphometric characteristics of tilapia were different in each treatment. The results of the simple linear regression test on the parameters of tilapia growth patterns in all treatments were negative allometric, namely the growth in length was faster than the growth in weight. The results of water quality measurements in all treatments showed results that were in accordance with quality standards for tilapia with a temperature range of 25,1-29,6 oC, dissolved oxygen range of 5,1-6,8 mg L-1, pH range of 7,42-8,14, and ammonia range of 0,01-0,17 mg L-1.
Co-Authors Adnan Wantasen Alex D. Kambey Ali Djamhuri Arne A Ratulangi Calvyn F. A. Sondak, Calvyn F. A. Cyska Lumenta Deiske Adeliene Sumilat, Deiske Adeliene Denovis Sambode Desmianti Babo Durand, Swenekhe S. Edwin D Ngangi Erly Kaligis Fanny Silooy Fintarji, Rian Frengky Ibo Gerung, Pramulya R.A Ginting, Elvie L. Grace O. Tambani Hengky J Sinjal Hengky J. Sinjal, Hengky J. Hengky Manoppo Hengky Sinjal Henky Manoppo Hety B Lahope Indra R. N. Salindeho Indra R.N. Salindeho Indra RN Salindeho Inneke F. M Rumengan Iroth, Revelino A. Jhonly Solang Joice R.T.S.L Rimper Joppy Mudeng Joula Kusen, Diane Juliaan Ch. Watung Julius Sampekalo Kalesaran, Ockstan Jurike Kreckhoff, Reni L. Kreckhoff, Reni Lucia Kurniati Kemer Kusen, Diane Joula Kusuma, Ni Putu Dian Lexy K. Rarung Mantiri, Rose Markus T. Lasut Mingkit, Winda M. Mokolensang, Jeffrie F. Mokolensang, Jeffrie F. F. Mokolensang, Jeffrie Fredrik Monijung, Revol D. Mudeng, Nico E.G. Nanariain, Rian M. Natalie D Rumampuk Ngangi, Edwin Leonardo Apolonio Ngatung, Jefrikardus E. E. Niti, Eukharistian Nowin, Edgar Ockstan J. Kalesaran Pamula, Doni F. Pangandaheng, Agreystin Pangemanan, Novie P.L. Pangemanan, Novie Pankie Lukas Pangemanan1, Novie P. L. Pankie N.L Pangemanan Paulus, James Petrus P Letsoin Reiny A. Tumbol Reiny Tumbol Sambali, Hariyani Sambali, Haryani Sambali, M.Sc, Hariyani Sammy N.J. Longdong Sanggaria, Ayu W. Saragih, Debby Dyanessa Sartje Lantu Simangunsong, Martua Pinondang Simanjorang, Daniel Simanjuntak, Erika E. L. Sinjal, Hengky Sinjal Sipriana S. Tumembouw Soputan, Stevaldo J.M. Stenly Wullur Sumah, Maria Suzanne L Undap Tamba, Ermaulina Tununu, Riky R. Undi, Chintia S. Utami, Tiara Veibe Warouw Victoria E.N. Manoppo, Victoria E.N. Wenda, Detiben Winda M. Mingkid, Winda M. Wulur, Stenly