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Journal : Sains dan Matematika

ANTIOXIDANT ACTIVITY OF JUICE OF LOCAL CULTIVAR SWEET POTATOES AS FREE RADICAL SCAVENGERS OF 1,1-DIPHENYL-2-PICRYLHYDRAZYL (DPPH) Palupi, Endah Sri; Mulyati-Sarto, Mulyati-Sarto; Pratiwi, Rarastoeti
Sains & Matematika Vol 1, No 1 (2012): Oktober, Sains & Matematika
Publisher : Sains & Matematika

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Abstract

Sweet potatoes (Ipomoea batatas) are tuber that have high antioxidant activity. The determination of antioxidant activity based on as percentage of inhibition of free radical DPPH. The purpose of this research was to observe the activity of three local cultivars of sweet potatoes by using DPPH method and by using vitamine C as compparison. The measurement of antioxidant activity which performed on sweet potatoes juice and vitamine C with concentration 625 ppm, 1250 ppm, 2500 ppm, 5000 ppm and 10000 ppm by using free radical of DPPH 0,004% that was soluble in ethanol pa and then was measure by using spectrofotometer. Data were analyzed by using ANOVA and LSD (? = 0.05). The result of this research showed that the three cultivar of sweet potatoes juice have antioxidant activity and the purple sweet potatoes cultivar Biru Mangsi had the highest antioxidant activity, although lower than vitamine C. The highest of antioxidant activity were vitamine C, followed by purple sweet potatoe, yellow sweet potatoes (cultivar Kuning Madu) and white sweet potatoes (cultivar Kapasan). 
KEKAYAAN JENIS LANDAK LAUT (ECHINOIDEA) FAMILI DIADEMATIDE DI PANTAI SELATAN KABUPATEN GUNUNG KIDUL Purnami, S.E.; Trijoko, Trijoko; Rarastoeti, Pratiwi
Sains & Matematika Vol 1, No 1 (2012): Oktober, Sains & Matematika
Publisher : Sains & Matematika

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Abstract

Diadematidae is one of sea urchin families belongs to Class Echinoidea that can be found in intertidal zone untill deep sea. These sea urchins have long spines, and some of them are toxic spines. However, they have important ecological roles. This research aimed to identify the species richness of sea urchins that belong to Family Diadematidae in Pantai Selatan Gunung Kidul as well as describe their taxonomic characters. Specimens were collected from five beaches, namely Krakal, Kukup, Drini, Sepanjang, and Wediombo. Collected specimens were relaxated by using MgCl272% in sea water and the fixed by using 10% formalin in sea water. Finally, they were preserved in 70% Ethanol. Some morphological characters were observed carefully, namely shape and colour of spines, shape of test, shape of genital plate, the existance of orange ring, white spot and iridipore of apical system, interambulacral region, and number of pores. The results showed that there were five species of Diadematid sea urchis in Pantai Selatan Yogyakarta; they belong to two genus, namely genus Diadema dan genus Echinotrix. Genus Echinotrix and Diadema can be fount in Kukup, Wediombo, and Sepanjang, but can not be found in two others beach. 
Antioxidant Activity of Juice of Local Cultivar Sweet Potatoes as Free Radical Scavengers of 1,1-diphenyl-2-picrylhydrazyl (DPPH) Endah Sri Palupi; Mulyati-Sarto Mulyati-Sarto; Rarastoeti Pratiwi
Sains dan Matematika Vol. 1 No. 1 (2012): Oktober, Sains & Matematika
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sweet potatoes (Ipomoea batatas) are tuber that have high antioxidant activity. The determination of antioxidant activity based on as percentage of inhibition of free radical DPPH. The purpose of this research was to observe the activity of three local cultivars of sweet potatoes by using DPPH method and by using vitamine C as compparison. The measurement of antioxidant activity which performed on sweet potatoes juice and vitamine C with concentration 625 ppm, 1250 ppm, 2500 ppm, 5000 ppm and 10000 ppm by using free radical of DPPH 0,004% that was soluble in ethanol pa and then was measure by using spectrofotometer. Data were analyzed by using ANOVA and LSD (α = 0.05). The result of this research showed that the three cultivar of sweet potatoes juice have antioxidant activity and the purple sweet potatoes cultivar Biru Mangsi had the highest antioxidant activity, although lower than vitamine C. The highest of antioxidant activity were vitamine C, followed by purple sweet potatoe, yellow sweet potatoes (cultivar Kuning Madu) and white sweet potatoes (cultivar Kapasan).